scholarly journals Nitric Oxide as an Attenuator of Ecophysiological Changes in Corn (Zea mays L.) Plants Submitted to Copper Toxicity

2021 ◽  
Vol 8 (4) ◽  
pp. 735
Author(s):  
Ana Ecidia de Araújo Brito ◽  
Jessica Suellen Silva Teixeira ◽  
Diana Jhulia Palheta de Sousa ◽  
Evelyn Fátima Lima de Souza ◽  
Gabriel Ito dos Santos Teixeira ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Zea Mays ◽  
Sodium Nitroprusside ◽  
Zea Mays L ◽  
Copper Toxicity ◽  
Maximum Activity ◽  
Nitric Oxide Donor ◽  
Water Control ◽  
Ps Ii ◽  
Corn Plants

The aim of this work was to evaluate the attenuating effect of the nitric oxide donor (sodium nitroprusside-NPS) on the ecophysiological responses of corn plants (Zea mays L.) submitted to copper toxicity. The corn seeds of the K9606 VIP3 variety were soaked for 48 hours in Germitest with solution containing treatment with sodium nitroprusside Na2 [Fe (CN) 5 NO] 2H2O (0, 200 and 300µM), sodium ferrocyanide Na4Fe (CN) 6 ( 300, 100 and 0 µM) respectively and deionized water (control), sown in buckets with 15 kg of soil incubated for 50 days containing copper concentrations CuSO4.5H2O (0, 60 and 200 mg kg-1). The design consisted of randomized blocks with 12 treatments and 4 repetitions, making a total of 48 plants. in ecophysiological variables: height, leaf area and number of leaves, stem diameter increased by 32, 66% and 11.29% in the treatments with 60 mg kg-1 of copper and 200 mg kg-1 of copper, respectively. There was no effect of treatments on the chlorophyll content measured by the SPAD index and gas exchange. The chlorophyll a fluorescence variables indicate that the concentration of 200 mg kg-1 of copper caused damage to the structure of the PS II reaction center complexes and indicate a slightly protective effect of nitric oxide-NO present in sodium nitroprusside and cyanide present in sodium ferrocyanide, reflecting a smooth functioning of the maximum activity of photosystem II and the electron transport chain.

Effects of the Nitric Oxide Donor Sodium Nitroprusside on Intracellular pH and Contraction in Hypertrophied Myocytes

Circulation ◽  
1997 ◽  
Vol 95 (9) ◽  
pp. 2303-2311 ◽  
Author(s):  
Nobuhiko Ito ◽  
Josef Bartunek ◽  
Kenneth W. Spitzer ◽  
Beverly H. Lorell
Keyword(s):  
Nitric Oxide ◽  
Sodium Nitroprusside ◽  
Intracellular Ph ◽  
Nitric Oxide Donor

scholarly journals Exogenous Nitric Oxide Confers Tolerance to Cr(VI) in Maize (Zea mays L.) Seedlings by Modulating Endogenous Oxido-Nitrosative Events

2021 ◽  
Author(s):  
Oussama Kharbech ◽  
Marouane Ben Massoud ◽  
Abdelilah Chaoui ◽  
Luis Alejandro Jose Mur ◽  
Wahbi Djebali
Keyword(s):  
Nitric Oxide ◽  
Zea Mays ◽  
Zea Mays L ◽  
Exogenous Nitric Oxide

The effect of the nitric oxide donor sodium nitroprusside on glucose uptake in human primary skeletal muscle cells

Nitric Oxide ◽  
2009 ◽  
Vol 21 (2) ◽  
pp. 126-131 ◽  
Author(s):  
Darren C. Henstridge ◽  
Brian G. Drew ◽  
Melissa F. Formosa ◽  
Alaina K. Natoli ◽  
David Cameron-Smith ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Sodium Nitroprusside ◽  
Glucose Uptake ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Nitric Oxide Donor

The Nitric Oxide Donor Sodium Nitroprusside Inhibits Mineralization in ATDC5 Cells

2006 ◽  
Vol 78 (3) ◽  
pp. 171-177 ◽  
Author(s):  
L. F. A. Huitema ◽  
A. B. Vaandrager ◽  
P. R. van Weeren ◽  
A. Barneveld ◽  
J. B. Helms ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Sodium Nitroprusside ◽  
Nitric Oxide Donor

Mechanisms underlying induction of heme oxygenase-1 by nitric oxide in renal tubular epithelial cells

2000 ◽  
Vol 279 (4) ◽  
pp. F728-F735 ◽  
Author(s):  
Mingyu Liang ◽  
Anthony J. Croatt ◽  
Karl A. Nath
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Sodium Nitroprusside ◽  
Nitric Oxide Donor ◽  
Heme Oxygenase 1 ◽  
Tubular Epithelial Cells ◽  
Cytotoxic Effects ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Renal Proximal Tubular

We examined whether nitric oxide-generating agents influence expression of heme oxygenase-1 (HO-1) in renal proximal tubular epithelial cells, LLC-PK1 cells, and the mechanisms underlying any such effects. In sublytic amounts, the nitric oxide donor sodium nitroprusside induced HO-1 mRNA and protein and HO activity in a dose-dependent and time-dependent fashion; this induction was specific for nitric oxide since the nitric oxide scavenger carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide significantly reduced such induction. The induction of HO activity by sodium nitroprusside, or by another nitric oxide donor, spermine NONOate, was markedly reduced by the iron chelator deferoxamine. Two different thiol-containing agents, N-acetylcysteine and dithiothreitol, blunted such induction of HO by nitric oxide. Downstream products of nitric oxide, such as peroxynitrite or cGMP, were not involved in inducing HO. In higher concentrations (millimolar amounts), sodium nitroprusside induced appreciable cytotoxicity as assessed by lactate dehydrogenase (LDH) release and lipid peroxidation, and both of these effects were markedly reduced by deferoxamine. Inhibition of HO did not affect the cytotoxic effects (measured by LDH release) of sodium nitroprusside. We thus provide the novel description of the induction of HO-1 in renal proximal tubular epithelial cells exposed to nitric oxide donors and provide the first demonstration in kidney-derived cells for the involvement of a redox-based mechanism in such expression. We also demonstrate that, in LLC-PK1 cells exposed to nitric oxide donors, chelatable iron is involved in eliciting the HO-1 response observed at lower concentrations of these donors, and in mediating the cytotoxic effects of these donors when present in higher concentrations.

scholarly journals SKRINNING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN DAN TABIR SURYA PADA TANAMAN JAGUNG (Zea mays L.)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 194
Author(s):  
David Albert Pangemanan ◽  
Edi Suryanto ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Zea Mays ◽  
Zea Mays L ◽  
Total Phenolic ◽  
Flavonoid Compounds ◽  
A Value ◽  
Maximum Protection ◽  
Area North ◽  
Corn Plants ◽  
Corn Kernels

ABSTRACT Corn (Zea Mays L.) is widely developed in Indonesia. Corn kernels showed total phenolic and antioxidant activity. Antioxidants have the potential as photoprotectors, therefore, plants containing phenolic compounds can be used in the prevention of free radicals. For this reason, researcher is interested in further researching the phytochemical screening, antioxidant activity and sunscreen testing from extracts of stems, leaves, hair and leaf wrapping from corn (Zea mays L.). The samples used were corn plants (corn silk, corn leaves, corn stalks and leaf wrapping corn) originating from Kauditan area, North Minahasa. The results of the study showed that the leaf contained saponin and flavonoid compounds, the stem contained alkaloid and saponin compounds, the hair contained alkaloid and flavonoid compounds, and the leaf wrapping only contained alkaloid compounds. The highest to lowest total phenolic content values starting from the stem were 46.93 μg / mL, the leaf wrapping 37.76 μg / mL, the leaf 26.63 μg / mL and hair 14.49 μg / mL. The leaf has the highest antioxidant activity with a value of 72.81% followed by 62.87% stem then wrapping leaf 43.13%  and finally hair 29.14%. The SPF value of the stem was included in the ultra protection with a value of 16.117 followed by the leaves included in the maximum protection with an SPF value of 10.902. While the hair with SPF value of 0.6 and wrapping leaf with SPF value of 0.222 are included in the minimum protection. Keywords: Corn (Zea Mays L.), Sunscreen, Antioxidants, Phenol ABSTRAK Jagung (Zea Mays L.) banyak dikembangkan di Indonesia. Biji jagung menunjukkan kadar total fenolik dan aktivitas antioksidan. Antioksidan memiliki potensi sebagai fotoprotektor, oleh karena itu, tanaman yang mengandung senyawa fenolik dapat digunakan dalam pencegahan radikal bebas. Untuk itu peneliti tertarik untuk meneliti lebih lanjut tentang skrining fitokimia, uji aktivitas antioksidan dan tabir surya dari ekstrak batang, daun, rambut, dan daun pembungkus dari jagung (Zea mays L.). Sampel yang digunakan ialah tanaman jagung (rambut jagung, daun jagung, batang jagung dan daun pembungkus jagung) yang berasal dari daerah Kauditan, Minahasa Utara. Hasil dari penelitian menunjukkan daun mengandung senyawa saponin dan flavonoid, batang mengandung senyawa alkaloid dan saponin, rambut mengandung senyawa alkaloid dan flavonoid, dan daun pembungkus hanya mengandung senyawa alkaloid. Nilai kandungan total fenolik  yang paling tinggi sampai paling rendah dimulai dari batang sebesar 46.93 μg/mL, daun pembungkus 37.76 μg/mL, daun 26.63 μg/mL dan rambut 14.49 μg/mL. Daun memiliki aktivitas antioksidan yang paling tinggi dengan nilai 72.81% diikuti oleh batang 62.87% kemudian daun pembungkus 43.13% dan yang terakhir adalah rambut 29.14%. Nilai SPF batang termasuk dalam proteksi ultra dengan nilai 16,117 diikuti oleh daun yang termasuk dalam proteksi maksimal dengan nilai SPF 10,902. Sedangkan rambut dengan nilai SPF  0,6 dan daun pembungkus dengan nilai SPF 0,222 termasuk dalam proteksi minimal. Kata Kunci : Jagung (Zea Mays L.), Tabir Surya, Antioksidan, Fenol

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