Transcriptional Regulation of Human GD3 Synthase (hST8Sia I) by Fenretinide in Human Neuroblastoma SH-SY-5Y Cells

The human neuroblastoma cell line CHP100 provides a useful model system in which to study the molecular mechanisms of transcriptional regulation of the low-affinity nerve growth factor receptor (NGFR) gene during neuronal development. Basic fibroblast growth factor (bFGF) induced morphological changes in CHP100 cells, including flattening of cell bodies and neurite outgrowth. bFGF also increased p75NGFR immunoreactivity, as assessed by immunocytochemistry, and increased p75NGFR mRNA levels, as assessed by Northern (RNA) blot analysis. A chimeric gene consisting of 6.7 kb of the 5'-flanking region of the human NGFR gene linked to the chloramphenicol acetyltransferase gene was constructed. In stable transformants of CHP100 cells, 10 ng of bFGF per ml induced an eightfold increase in chloramphenicol acetyltransferase activity. These results indicate that upstream elements of the NGFR gene mediate transcriptional regulation by bFGF.

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Genomic Organization and Transcriptional Regulation of the Mouse GD3 Synthase Gene (ST8Sia I): Comparison of Genomic Organization of the Mouse Sialyltransferase Genes

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a022831 ◽

2000 ◽

Vol 128 (6) ◽

pp. 1033-1043 ◽

Cited By ~ 20

Author(s):

S. Takashima ◽

M. Kono ◽

N. Kurosawa ◽

Y. Yoshida ◽

Y. Tachida ◽

...

Keyword(s):

Transcriptional Regulation ◽

Genomic Organization ◽

Gd3 Synthase

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Valproic acid induces transcriptional activation of human GD3 synthase (hST8Sia I) in SK-N-BE(2)-C human neuroblastoma cells

Molecules and Cells ◽

10.1007/s10059-009-0012-4 ◽

2008 ◽

Vol 27 (1) ◽

pp. 113-118 ◽

Cited By ~ 13

Author(s):

Haw-Young Kwon ◽

Hyun-Mi Dae ◽

Na-Ri Song ◽

Kyoung-Sook Kim ◽

Cheorl-Ho Kim ◽

...

Keyword(s):

Valproic Acid ◽

Transcriptional Activation ◽

Neuroblastoma Cells ◽

Human Neuroblastoma Cells ◽

Human Neuroblastoma ◽

Gd3 Synthase

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PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells

Laboratory Investigation ◽

10.1038/s41374-020-0462-z ◽

2020 ◽

Vol 100 (12) ◽

pp. 1551-1563

Author(s):

Ikumi Kitazono ◽

Taiji Hamada ◽

Takuya Yoshimura ◽

Mari Kirishima ◽

Seiya Yokoyama ◽

...

Keyword(s):

Transcriptional Regulation ◽

Neurite Outgrowth ◽

Human Neuroblastoma

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Basic fibroblast growth factor enhances nerve growth factor receptor gene promoter activity in human neuroblastoma cell line CHP100.

Molecular and Cellular Biology ◽

10.1128/mcb.12.5.2193 ◽

1992 ◽

Vol 12 (5) ◽

pp. 2193-2202 ◽

Cited By ~ 11

Author(s):

M Taiji ◽

K Taiji ◽

K L Deyerle ◽

M Bothwell

Keyword(s):

Transcriptional Regulation ◽

Nerve Growth Factor ◽

Growth Factor ◽

Neuroblastoma Cell ◽

Growth Factor Receptor ◽

Neuroblastoma Cell Line ◽

Human Neuroblastoma Cell ◽

Fibroblast Growth ◽

Human Neuroblastoma ◽

Human Neuroblastoma Cell Line

The human neuroblastoma cell line CHP100 provides a useful model system in which to study the molecular mechanisms of transcriptional regulation of the low-affinity nerve growth factor receptor (NGFR) gene during neuronal development. Basic fibroblast growth factor (bFGF) induced morphological changes in CHP100 cells, including flattening of cell bodies and neurite outgrowth. bFGF also increased p75NGFR immunoreactivity, as assessed by immunocytochemistry, and increased p75NGFR mRNA levels, as assessed by Northern (RNA) blot analysis. A chimeric gene consisting of 6.7 kb of the 5'-flanking region of the human NGFR gene linked to the chloramphenicol acetyltransferase gene was constructed. In stable transformants of CHP100 cells, 10 ng of bFGF per ml induced an eightfold increase in chloramphenicol acetyltransferase activity. These results indicate that upstream elements of the NGFR gene mediate transcriptional regulation by bFGF.

Download Full-text