gd3 synthase
Recently Published Documents


TOTAL DOCUMENTS

84
(FIVE YEARS 7)

H-INDEX

22
(FIVE YEARS 1)

2021 ◽  
Author(s):  
Yuki Ohkawa ◽  
Pu Zhang ◽  
Hiroyuki Momota ◽  
Akira Kato ◽  
Noboru Hashimoto ◽  
...  

2021 ◽  
Author(s):  
Carla Andreia Abreu ◽  
Leandro Coelho Teixeira‐Pinheiro ◽  
Rafael Lani‐Louzada ◽  
Almir Jordão da Silva‐Junior ◽  
Juliana F. Vasques ◽  
...  

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rika Takeuchi ◽  
Mariko Kambe ◽  
Maiko Miyata ◽  
Upul Jeyadevan ◽  
Orie Tajima ◽  
...  

Abstract Analyses of expression and regulation of ganglioside synthases in melanocytes are important to understand roles of gangliosides in melanomagenesis. In this study, we analyzed the expression and regulatory mechanisms of glycosyltransferase genes responsible for ganglioside synthesis in normal melanocytes. We reported previously that culture supernatants of UVB-irradiated keratinocytes induced upregulation of ganglioside GD3 synthase gene in melanocytes, and mainly TNFα was responsible for it. Then, we found that elimination of dibutyryl cyclic AMP and IBMX from the medium also resulted in upregulation of the GD3 synthase gene. The addition of α-melanocyte-stimulating hormone which increases cAMP, to the medium led to a significant reduction in the GD3 synthase gene expression level, and a PKA inhibitor enhanced the GD3 synthase gene level. These results suggest that signals mediated via TNFα and cAMP oppositely regulate GD3 synthase gene expression in melanocytes. The results of an IKK inhibitor indicate the possibility that TNFα induces GD3 synthase gene expression via NF-κB signaling in melanocytes. When melanoma cells were treated by these factors, no fluctuation in the GD3 synthase gene expression level was observed, although an IKK inhibitor significantly suppressed it, suggesting that ganglioside synthase genes are regulated in distinct manners between melanocytes and melanomas.


2019 ◽  
Author(s):  
Appalaraju Jappupilli ◽  
Khoa Nguyen ◽  
Stanley Ly ◽  
Michael Andreeff ◽  
Prashen Chelikani ◽  
...  

2019 ◽  
Vol 20 (11) ◽  
pp. 2825 ◽  
Author(s):  
Shoyoku Yo ◽  
Kazunori Hamamura ◽  
Yoshitaka Mishima ◽  
Kosuke Hamajima ◽  
Hironori Mori ◽  
...  

Gangliosides are widely expressed in almost all tissues and cells and are also considered to be essential in the development and maintenance of various organs and tissues. However, little is known about their roles in bone metabolism. In this study, we investigated the effects of genetic deletion of ganglioside D3 (GD3) synthase, which is responsible for the generation of all b-series gangliosides, on bone metabolism. Although b-series gangliosides were not expressed in osteoblasts, these gangliosides were expressed in pre-osteoclasts. However, the expression of these gangliosides was decreased after induction of osteoclastogenesis by receptor activator of nuclear factor kappa-B ligand (RANKL). Three-dimensional micro-computed tomography (3D-μCT) analysis revealed that femoral cancellous bone mass in GD3 synthase-knockout (GD3S KO) mice was higher than that in wild type (WT) mice at the age of 40 weeks, although there were no differences in that between GD3S KO and WT mice at 15 weeks old. Whereas bone formation parameters (osteoblast numbers/bone surface and osteoblast surface/bone surface) in GD3S KO mice did not differ from WT mice, bone resorption parameters (osteoclast numbers/bone surface and osteoclast surface/bone surface) in GD3S KO mice became significantly lower than those in WT mice at 40 weeks of age. Collectively, this study demonstrates that deletion of GD3 synthase attenuates bone loss that emerges with aging.


2019 ◽  
Vol 692 ◽  
pp. 53-63 ◽  
Author(s):  
Anandh Dhanushkodi ◽  
Yi Xue ◽  
Emily E. Roguski ◽  
Yun Ding ◽  
Shannon G. Matta ◽  
...  

2018 ◽  
Vol 8 (5) ◽  
pp. 713-720 ◽  
Author(s):  
Jinyi Liu ◽  
Xiangjin Zheng ◽  
Xiaocong Pang ◽  
Li Li ◽  
Jinhua Wang ◽  
...  

Author(s):  
Miri Lee ◽  
Kyoung-Sook Kim ◽  
Dong-Hyun Kim ◽  
Cheorl-Ho Kim ◽  
Young-Choon Lee

Curcumin, a natural polyphenolic compound isolated from the plant Curcuma longa, is known to induce autophagy in various cancer cells, including lung cancer. In the present study, we also confirmed by LC3 immunofluorescence and immunoblotting analyses that curcumin triggers autophagy in human lung adenocarcinoma A549 cell line. In parallel with autophagy induction, gene expression of human GD3 synthase (hST8Sia I) responsible for ganglioside GD3 synthesis was markedly elevated in response to curcumin in A549 cells. To investigate transcriptional activation of hST8Sia I associated with autophagy formation in curcumin-treated A549 cells, functional characterization of the 5’-flanking region of the hST8Sia I gene was carried out using luciferase reporter assay system. Deletion analysis demonstrated that the -1146 to -646 region, which includes putative c-Ets-1, CREB, AP-1 and NF-κB binding sites, functions as the curcumin-responsive promoter of hST8Sia I in A549 cells. Site-directed mutagenesis and chromatin immunoprecipitation assay demonstrated that the NF-κB binding site at -731 to -722 was indispensable for the curcumin-induced hST8Sia I gene expression in A549 cells. Moreover, the transcriptional activation of hST8Sia I by curcumin A549 cells was strongly inhibited by compound C, an inhibitor of AMP-activated protein kinase (AMPK). These results suggest that curcumin controls hST8Sia I gene expression via AMPK signal pathway in A549 cells.


Sign in / Sign up

Export Citation Format

Share Document