LIPID NANOPARTICLES FOR TRANSDERMAL DELIVERY OF CELECOXIB: AN IN VITRO AND IN VIVO INVESTIGATION

In the present research work, celecoxib (CXB) loaded solid lipid nanoparticles (SLNs) were prepared using the probe sonication method, wherein Glyceryl monostearate and Tween 80 were used as solid lipid and surfactant, respectively. To obtain the statistically optimized batch, 32 factorial design was applied. The optimized batch was characterized physicochemically and evaluated through DSC, SEM and XRD studies. The mean particle size of the optimized batch was found to be 135.41± 0.24 nm with a mean % entrapment efficiency of 80 ± 1.69%. The optimized batch was further lyophilized and dispersed into 1% w/v Carbopol 934P to form a gel. Prepared gel was further evaluated for in vitro drug release, occlusivity, ex vivo permeability, local toxicity, in vivo anti-inflammatory activity and accelerated stability study. The study resulted in stable, safe and prolonged anti-inflammatory activity with quick onset of action. Hence, celecoxib loaded solid lipid nanoparticles can be considered as promising alternative to conventional topical systems.

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Solid lipid nanoparticles (SLN) as carriers for the topical delivery of econazole nitrate: in-vitro characterization, ex-vivo and in-vivo studies

Journal of Pharmacy and Pharmacology ◽

10.1211/jpp.59.8.0002 ◽

2007 ◽

Vol 59 (8) ◽

pp. 1057-1064 ◽

Cited By ~ 73

Author(s):

Vanna Sanna ◽

Elisabetta Gavini ◽

Massimo Cossu ◽

Giovanna Rassu ◽

Paolo Giunchedi

Keyword(s):

Solid Lipid Nanoparticles ◽

Ex Vivo ◽

Topical Delivery ◽

Lipid Nanoparticles ◽

In Vivo Studies ◽

Solid Lipid ◽

In Vitro Characterization ◽

Econazole Nitrate

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Erratum to "Nanotechnology in Wound Healing; Semisolid Dosage Forms Containing Curcumin-Ampicillin Solid Lipid Nanoparticles, in Vitro, Ex Vivo and in Vivo Characteristics"

Advanced Pharmaceutical Bulletin ◽

10.34172/apb.2021.088 ◽

2020 ◽

Vol 11 (1) ◽

pp. 204-204

Author(s):

Solmaz Ghaffari ◽

Faezeh Alihosseini ◽

Seyed Mahdi Rezayat Sorkhabadi ◽

Sepideh Arbabi Bidgoli ◽

Seyyedeh Elaheh Mousavi ◽

...

Keyword(s):

Wound Healing ◽

Solid Lipid Nanoparticles ◽

Ex Vivo ◽

Lipid Nanoparticles ◽

Dosage Forms ◽

Solid Lipid

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Preparation, characterization, and optimization of auraptene-loaded solid lipid nanoparticles as a natural anti-inflammatory agent: In vivo and in vitro evaluations

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2018.01.054 ◽

2018 ◽

Vol 164 ◽

pp. 332-339 ◽

Cited By ~ 8

Author(s):

Sara Daneshmand ◽

Mahmoud Reza Jaafari ◽

Jebrail Movaffagh ◽

Bizhan Malaekeh-Nikouei ◽

Mehrdad Iranshahi ◽

...

Keyword(s):

Solid Lipid Nanoparticles ◽

Lipid Nanoparticles ◽

Solid Lipid ◽

Inflammatory Agent ◽

Anti Inflammatory

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TOPICAL SOLID LIPID NANOPARTICLES BASED GEL OF LAVENDER ESSENTIAL OIL FOR ANTI‑INFLAMMATORY ACTIVITY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i11.35459 ◽

2019 ◽

pp. 175-182

Author(s):

PALLAVI M CHAUDHARI ◽

VAISHNAVI M BIND

Keyword(s):

Particle Size ◽

Drug Release ◽

Essential Oil ◽

Solid Lipid Nanoparticles ◽

Ex Vivo ◽

Entrapment Efficiency ◽

Lipid Nanoparticles ◽

Inflammatory Activity ◽

Solid Lipid ◽

Anti Inflammatory

Objective: The main objective of the study was to formulate and evaluate and perform an optimization study of lavender essential oil loaded solid lipid nanoparticles (SLNs) based gel. Materials and Methods: SLNs were prepared by the hot homogenization technique. A total of eight formulations were formulated as per 23 factorial design by design expert 11 software. The formulated SLNs were further evaluated for particle size, entrapment efficiency, drug release profile. After evaluation, the optimized batch was further used for formulating gel. The formulated gel was further subjected to ex vivo studies. Results: After the evaluation of all the parameters, batch 7 was found to be optimized. Batch 7 was found to have the lowest particle size of 30.91±0.30, higher entrapment efficiency of 89.99±0.87, and higher drug release of 90.41±0.55. It was further used for formulating gel which was found to be consistent, homogenous, smooth, and spreadable. The % inhibition of the formulated SLN based gel was found to be 28±0.1%. Conclusion: The SLNs were prepared and were formulated into the gel. The gel showed anti-inflammatory activity.

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Solid Lipid Nanoparticles of Guggul Lipid as Drug Carrier for Transdermal Drug Delivery

BioMed Research International ◽

10.1155/2013/750690 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 18

Author(s):

Praveen Kumar Gaur ◽

Shikha Mishra ◽

Suresh Purohit

Keyword(s):

Drug Release ◽

Solid Lipid Nanoparticles ◽

Lipid Nanoparticles ◽

Inflammatory Activity ◽

Physical Parameters ◽

In Vitro Drug Release ◽

Solid Lipid ◽

Lipid Component ◽

Anti Inflammatory

Diclofenac sodium loaded solid lipid nanoparticles (SLNs) were formulated using guggul lipid as major lipid component and analyzed for physical parameters, permeation profile, and anti-inflammatory activity. The SLNs were prepared using melt-emulsion sonication/low temperature-solidification method and characterized for physical parameters, in vitro drug release, and accelerated stability studies, and formulated into gel. Respective gels were compared with a commercial emulgel (CEG) and plain carbopol gel containing drug (CG) for ex vivo and in vivo drug permeation and anti-inflammatory activity. The SLNs were stable with optimum physical parameters. GMS nanoparticle 1 (GMN-1) and stearic acid nanoparticle 1 (SAN-1) gave the highest in vitro drug release. Guggul lipid nanoparticle gel 3 (GLNG-3) showed 104.68 times higher drug content than CEG in receptor fluid. The enhancement ratio of GLNG-3 was 39.43 with respect to CG. GLNG-3 showed almost 8.12 times higherCmaxthan CEG at 4 hours. The AUC value of GLNG-3 was 15.28 times higher than the AUC of CEG. GLNG-3 showed edema inhibition up to 69.47% in the first hour. Physicochemical properties of major lipid component govern the properties of SLN. SLN made up of guggul lipid showed good physical properties with acceptable stability. Furthermore, it showed a controlled drug release profile along with a promising permeation profile.

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In Vitro, Ex Vivo, Penetration (EpiDermTM) and in Vivo Dermatokinetics of Ketoconazole Loaded Solid Lipid Nanoparticles for Topical Delivery

10.21203/rs.3.rs-408418/v1 ◽

2021 ◽

Author(s):

Mohhammad Ramzan ◽

Samuel Gourion-Arsiquaud ◽

Afzal Hussain ◽

Jaspreet Singh Gulati ◽

Qihong Zhang ◽

...

Keyword(s):

Solid Lipid Nanoparticles ◽

Ex Vivo ◽

Lipid Nanoparticles ◽

Laser Scanning Microscopy ◽

Pharmacokinetic Parameters ◽

Rat Skin ◽

Solid Lipid ◽

Ex Vivo Permeation

Abstract The study focused to optimize, evaluate and investigate mechanistic perspective of ketoconazole (KTZ) loaded solid lipid nanoparticles (KTZ-SLNs) for enhanced permeation across rat skin. KTZ-SLNs were evaluated for size, distribution, zeta potential (ZP), percent entrapment efficiency (%EE), drug release, morphology, thermal behavior (DSC), compatibility (FTIR) and solid state characterization (X-ray diffraction, XRD). Moreover, ex-vivo permeation and drug deposition into rat skin were conducted using Franz diffusion cell. Mechanistic evaluations were confirmed using confocal laser scanning microscopy and vibrational ATR methods using EpiDermTM model. An in vivo dermatokinetics study was performed to ensure KTZ access to the dermal region. Accelerated and photostability studies were conducted at different temperatures (0, 30, and 40 °C) for 12 months. The spherical optimized KOF1 showed optimal particle size (291 nm), and high negative ZP (-27.7 mV). Results of FTIR, DSC, and XRD confirmed compatibility of KTZ with excipients, purity of KTZ & dissolved KTZ in lipid matrix, and amorphous nature of KTZ-SLNs. In-vitro release was found to be slow and sustained whereas ex vivo permeation parameters were significantly high in KTZ-SLNs as compared to drug suspension and marketed product. Drug retention was 10- and -5 fold higher than KTZ-SUS and marketed product, respectively. Pharmacokinetic parameters were improved by SLNs formulation. Confocal raman spectroscopy experiment showed that KTZ-SLNs could penetrate beyond the human stratum corneum into viable epidermis. Fluorescent microscopy confirmed improved penetration of KTZ-SLNs was through human follicular pathway. KTZ-SLNs stable over 12 months under set conditions.

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