scholarly journals Characterization of Petroleum Hydrocarbon Decomposing Fungi Isolated from Mangrove Rhizosphere

2013 ◽  
Vol 16 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Nuni Gofar
Keyword(s):  
Petroleum Hydrocarbon ◽  
Soil Samples ◽  
Petroleum Residue ◽  
Total Petroleum Hydrocarbon ◽  
Aspergillus Fumigates ◽  
Polycyclic Aromatic ◽  
Chrysonilia Sitophila ◽  
Soil Borne Fungi

The research was done to obtain the isolates of soil borne fungi isolated from mangrove rhizosphere which were capable of degrading petroleum hydrocarbon compounds. The soil samples were collected from South Sumatra mangrove forest which was contaminated by petroleum. The isolates obtained were selected based on their ability to survive, to grow and to degrade polycyclic aromatic hydrocarbons in medium containing petroleum residue. There were 3 isolates of soil borne hydrocarbonoclastic fungi which were able to degrade petroleum in vitro. The 3 isolates were identified as Aspergillus fumigates, A. parasiticus, and Chrysonilia sitophila. C. sitophila was the best isolate to decrease total petroleum hydrocarbon (TPH) from medium containing 5-20% petroleum residue.Keywords: Hydrocarbonoclastic fungi, hydrocarbon compounds, mangrove rhizosphere

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

scholarly journals Isolation and Characterization of Carbonoclastic Bacteria Diversity in Oil-contaminated Soil in Cape Coast Metropolis, Ghana

2020 ◽  
pp. 62-74
Author(s):  
H. D. Nyarko ◽  
G. C. Okpokwasili ◽  
O. F. Joel ◽  
I. A. K. Galyuon
Keyword(s):  
Petroleum Hydrocarbon ◽  
Heterotrophic Bacteria ◽  
Soil Samples ◽  
Cape Coast ◽  
Bacterial Populations ◽  
Isolation And Characterization ◽  
Sampling Locations ◽  
Degrading Bacteria ◽  
Polluted Sites

Aims: The study aimed at the quantification, isolation and characterization of hydrocarbon degrading bacteria in oil-contaminated and pristine soils. Methodology: Soil samples from petroleum hydrocarbon polluted sites at auto-mechanic workshops, a mechanic village, as well as pristine (control) soils, comprising of 14 sampling locations within Cape Coast Metropolis in the Central Region of Ghana were collected using standard sampling techniques. Collected soil samples were treated and cultured while enumerations, isolations and characterization of carbonoclastic bacteria associated were evaluated. Results: Bacterial populations isolated from hydrocarbon-polluted sites had higher aerobic counts ranging from 7.24-8.02 log10 cfu/g of soil when compared with the pristine sites (from 6.79-7.61 log10 cfu/g of soil). Also, soil samples from the mechanic village (8.76 to 7.48 log10 cfu/g of soil) recorded more bacterial counts than those from the mechanic garages (8.02 to 7.24 log10 cfu/g of soil). The calculated percentage profiles of all the hydrocarbon utilizing bacteria in the total culturable heterotrophic bacteria were low throughout the study, even though the percentage scores were all above 50%. A total of 19 hydrocarbon degraders were isolated. The isolates identified belong to the genera Pseudomonas, Proteus, Bacillus and Enterobacter. Conclusion: The outcome of the study based on the bacteria populations, identification profiles, coupled with their survival and multiplications in designated medium amended with crude oil as the carbon and energy sources, suggest their petroleum hydrocarbon degrading capabilities, hence may be used in bioremediation applications.

scholarly journals Factorial design study of total petroleum contaminated soil treatment using land farming technique

2020 ◽  
Author(s):  
Ehizonomhen Solomon Okonofua ◽  
KAYODE HASSAN LASISI ◽  
Eguakhide Atikpo
Keyword(s):  
Moisture Content ◽  
Factorial Design ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbon ◽  
Optimum Point ◽  
Input Parameters ◽  
Substrate Moisture ◽  
Land Farming

Abstract Land farming technique was used to treat hydrocarbon contaminated soil collected from a crude oil spill sites in Edo State, Nigeria. Calibrated standard auger was used to collect soil samples from the site at depth below 30 cm. The samples were characterized and classified. Cow dung and NPK fertilizer were added as additives to complement the nutriments of the soil samples before total petroleum hydrocarbon quantification and remediation procedures. Factorial design was applied to vary the input parameters such as pH, mass of substrate, moisture content and turning times of land farming so to ascertain the optimal conditions for the procedure. The result revealed that the in-situ total petroleum hydrocarbon (TPH) value was 5,000 mg kg-1 on the average and after 90 days of treatment, TPH reduced to 645.907 mg kg-1. The turning rate, pH, moisture content and mass of substrate had 82.79%, 4.36%, 0.48% and 0.046% contributions respectively to the degradation process using land farming treatment. Numerical optimization techniques applied in the optimum point for land farming input parameters to achieve predicted maximum removal of 98.60% were evaluated as pH, mass of substrate, moisture content and turning rate to be 6.01, 1 kg, 10% and 5 times in a week respectively. TPH removed at this optimum point was 97.83% reducing from 5,000 to 635.907 mg kg-1. The high coefficient of determination (r2 = 0.9865) as observed in the closeness of predicted and experimental values reflects the reliability of the model and hence, land farming practice with close attention on turning rate as revealed by this study, is recommended for TPH contaminated soil remediation.

scholarly journals Bioremediation of Soil Contaminated with Spent Engine Oil using Pig Dung

2021 ◽  
pp. 68-78
Author(s):  
A. A. Ibiene ◽  
O. Aleruchi ◽  
L.C. Nnodim ◽  
R. U. Ihunwo
Keyword(s):  
Contaminated Soil ◽  
Physicochemical Parameters ◽  
Petroleum Hydrocarbon ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbon ◽  
Engine Oil ◽  
Microbiological Analysis ◽  
Ph Values ◽  
Port Harcourt ◽  
Spent Engine Oil

Studies were carried out to investigate the bioremediation potential of pig dung in a soil contaminated with spent engine oil. Soil samples were obtained from the Ofrima complex, University of Port Harcourt. The soil samples were contaminated with various concentrations (50 ml and 100 ml) of spent engine oil and allowed for 21 days for proper exposure, mimicking natural spill. This was followed by the addition of the pig dung. The experimental setup was labeled sample A (1 kg soil + 100 g pig dung + 50 ml spent engine oil) and sample B (1 kg soil + 100 g pig dung + 100 ml spent engine oil). The physicochemical parameters and the microbiological analysis were done using standard methods. The total petroleum hydrocarbon was analyzed using gas chromatographic methods. Analyses were carried out at 14 days intervals for 28 days. The physicochemical parameter results showed a reduction in pH values in the contaminated soil samples, ranging from 6.21 - 6.65 in sample A and 6.57 - 6.87 in sample B. Temperature values were constant at 230C from day 1 to day 14 in sample A and increased at day 28 to 24 0C, also for sample B, the temperature was constant at 230C from day 1 to day14 and increased at day 28 to 26 0C. The amount of heavy metal (Lead) content decreased from 4.3645 - 1.93676 (mg/kg) and 6.18361 - 3.89654 (mg/kg) for samples A and B, respectively. There was also a significant reduction in the amount of Total Petroleum Hydrocarbon, from 16631.86 - 3280.83 mg/kg for sample A and 18464.73 - 6784.60 mg/kg for sample B. The THB counts for samples A and B ranged from 7.73 - 7.91 and 7.05-8.20 (Log cfu/g), respectively. The fungal counts ranged from 3.99–4.58 and 5.12 - 7.93 (Log cfu/g) for samples A and B respectively. HUB counts ranged from 4.52–5.09 and 4.93- 5.55 (Log cfu/g) for samples A and B, respectively. The HUF counts ranged from 4.12 - 5.49 and 4.13 - 4.70 (Log cfu/g) for samples A and B, respectively. The results clearly showed that microorganisms capable of utilizing total petroleum hydrocarbon were present, also the pig dung showed both bio-stimulation and bio-augmentation tendency to attract high microbial load which supported the bioremediation of the spent engine oil contaminated soil.

scholarly journals Biotreatment of Crude Oil Contaminated Soil

2019 ◽  
pp. 1-9 ◽  
Author(s):  
B. M. Popoola ◽  
A. A. Olanbiwonninu
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbon ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbon ◽  
Available Phosphorus ◽  
Subsequent Increase ◽  
A Value ◽  
Treatment Measures

Biodegradation of hydrocarbons by microorganisms represents one of the primary mechanisms by which petroleum and other hydrogen pollutants are eliminated from the environment. This work was carried out on the effect of microorganisms on the biotreatment of oil in crude oil contaminated soil. Microorganisms were isolated from two experimental soil samples contaminated with Bonny Crude and normal uncontaminated soil as a control over a period of seven months. The microbial as well as the physico-chemical parameters of the soil samples were all analyzed using standard methods. Changes in total petroleum hydrocarbon level were measured appropriately. Treatments used were the microbial isolates. Forty-four microorganisms were isolated from the contaminated soils and identified as species of Pseudomonas (7), Flavobacterium (6), Bacillus (8), Proteus (4), Klebsiella (1), Pencillium (5), Aspergillus (7), Fusarium (3), Trichypton (2) and Neurospora (1). Ten of the forty-four isolates had ability to degrade crude oil in the laboratory. On contamination a value of 1.0X105 cfu/g in microbial counts were obtained followed by a subsequent increase in population levels after a period of 2months with a value of 1.0X106 cfu/g. Oil application to the soil resulted in an increase in total petroleum hydrocarbon from 0.31 ppm to 5.53 ppm; organic matter from 0.41% to 7.34%; available phosphorus from 1.75 ppm to 2.84 ppm. The treatment measures all showed progressive decrease in oil concentration in the soil. Mixture of bacterial and fungal isolates as a treatment measure proved to be more favourable above all others, it brought the concentration from 5.53 ppm to 0.31 ppm after a period of 5 weeks of treatment, which is same value with the normal soil (uncontaminated). Species of Pseudomonas, Bacillus, Flavobacterium, Proteus, Klebsiella, Penicillium, Aspergillus, Fusarium, Trichyphyton and Neurospora had potential for the degradation of bonny crude oil. They could therefore be employed in environmental cleanup of petroleum spill site.

scholarly journals Factorial design study of total petroleum contaminated soil treatment using land farming technique

2021 ◽  
Author(s):  
Ehizonomhen Solomon Okonofua ◽  
Kayode H. Lasisi ◽  
Eguakhide Atikpo
Keyword(s):  
Moisture Content ◽  
Factorial Design ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbon ◽  
Optimum Point ◽  
Input Parameters ◽  
Substrate Moisture ◽  
Land Farming

Abstract Land farming technique was used to treat hydrocarbon contaminated soil collected from a crude oil spill sites in Edo State, Nigeria. Calibrated standard auger was used to collect soil samples from the site at depth below 30 cm. The samples were characterized and classified. Cow dung and NPK fertilizer were added as additives to complement the nutriments of the soil samples before total petroleum hydrocarbon quantification and remediation procedures. Factorial design was applied to vary the input parameters such as pH, mass of substrate, moisture content and turning times of land farming so to ascertain the optimal conditions for the procedure. The result revealed that the in-situ total petroleum hydrocarbon (TPH) value was 5,000 mg kg -1 on the average and after 90 days of treatment, TPH reduced to 645.907 mg kg -1 . The turning rate, pH, moisture content and mass of substrate had 82.79%, 4.36%, 0.48% and 0.046% contributions respectively to the degradation process using land farming treatment. Numerical optimization techniques applied in the optimum point for land farming input parameters to achieve predicted maximum removal of 98.60% were evaluated as pH, mass of substrate, moisture content and turning rate to be 6.01, 1 kg, 10% and 5 times in a week respectively. TPH removed at this optimum point was 97.83% reducing from 5,000 to 635.907 mg kg -1 . The high coefficient of determination (r 2 = 0.9865) as observed in the closeness of predicted and experimental values reflects the reliability of the model and hence, land farming practice with close attention on turning rate as revealed by this study, is recommended for TPH contaminated soil remediation.

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