scholarly journals Features of sodium magnetic resonance spectroscopy and its application in neurology

2021 ◽  
Vol 15 (3) ◽  
pp. 72-79
Author(s):  
Viktoriya V. Sinkova ◽  
Irina A. Krotenkova ◽  
Alina A. Lyaskovik ◽  
Rodion N. Konovalov ◽  
Marina V. Krotenkova
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Treatment Efficacy ◽  
Ion Homeostasis ◽  
Resonance Spectroscopy ◽  
Degenerative Diseases ◽  
Non Invasive ◽  
Central Nervous System Disorders ◽  
Invasive Method

Magnetic resonance spectroscopy is an important non-invasive method that measures concentration and spatial distribution of certain biochemically significant tissue metabolites. This relatively new method has now evolved from a research tool to an independent diagnostic neuroimaging method, which provides answers to a number of important clinical and diagnostic questions at the early stages of the disease, and allows evaluation of treatment efficacy and determination of clinical outcome. The article provides a review of data on sodium magnetic resonance spectroscopy, which is a very sensitive method for assessing cell viability and ion homeostasis. It can be used to measure early biochemical disturbances in the tissues in various degenerative diseases. We describe pathophysiology and technology underlying sodium magnetic resonance spectroscopy, as well as the most promising points of application of this method in central nervous system disorders seen by radiologists and neurologists in their clinical practice.

scholarly journals An Early Detection of Acute Compartment Syndrome in Fastened Zip-Tie Rat Model Using Dynamic Phosphorous-31 Magnetic Resonance Spectroscopy at 9.4 Tesla

2021 ◽  
Author(s):  
Hiroki Ohta ◽  
Nhat-Minh Van Vo ◽  
Junichi Hata ◽  
Koshiro Terawaki ◽  
Takako Shirakawa ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Early Detection ◽  
Magnetic Resonance Spectroscopy ◽  
Compartment Syndrome ◽  
Rat Model ◽  
Resonance Spectroscopy ◽  
Non Invasive ◽  
Arterial Ph ◽  
Invasive Method ◽  
Muscular Damage

Abstract IntroductionAcute compartment syndrome (ACS) leads to a series of health problems, limb salvage, disability, and even death. In vivo phosphorus-31 magnetic resonance spectroscopy (31P-MRS) provides a unique non-invasive method to assess skeletal muscle metabolisms such as inorganic phosphate (Pi), phosphocreatine (PCr), and adenosine triphosphate (ATP). The study aims to assess the ability of dynamic 31P-MRS in the early detection of muscular damage in ACS.Materials & MethodsThe study induced the fastened zip-tie model of ACS on normotensive Sprague-Dawley rats (n = 6). The spectra were acquired in Bruker 9.4-Tesla preclinical scanner using 1H/31P surface coil. 31P-MRS spectra and blood samples were obtained at time 0 (pre-ischemic phase) and every 15 minutes during the compression (120 minutes) and the reperfusion phase (90 minutes). 31P-MRS spectra findings were compared with plasma creatine phosphokinase (CPK).ResultsPCr/(Pi + PCr) ratio significantly decreased after muscle was compressed (P < 0.05). In contrast to this, CPK did not change significantly (P > 0.05). Both intracellular pH and arterial pH decreased over time. However, intracellular declined significantly (P < 0.05) at 60 minutes of ischemic state, and at 5 minutes and 60 minutes of reperfusion, while arterial pH slightly changed. After 30 minutes of ischemic, phosphomonoesters (PME) peak was detected, which was not seen at the pre-ischemic phase. It gradually increased and reached its highest peak at 120 minutes. At reperfusion state, 31P-MRS spectra and pH did not fully recover to their pre-ischemic state, and PME peak disappeared. There was a correlation between T2-weighted images and CPK from blood tests (R2 = 0.1996, P < 0.05).ConclusionsDynamic 31P-MRS technique is more clearly and rapidly detect the bioenergetic and mitochondrial functions change than blood test in a fastened zip-tie rat model of ACS. This technique is a promising non-invasive method to detect the early ischemic muscular damage in ACS.

scholarly journals Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Vinicius da Eira Silva ◽  
Vitor de Salles Painelli ◽  
Samuel Katsuyuki Shinjo ◽  
Wagner Ribeiro Pereira ◽  
Eduardo Maffud Cilli ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Muscle Carnosine ◽  
Non Invasive ◽  
Validity Assessment ◽  
Invasive Method

scholarly journals BIMG-08. DEUTERIUM MAGNETIC RESONANCE SPECTROSCOPY USING 2H-PYRUVATE ALLOWS NON-INVASIVE IN VIVO IMAGING OF TERT EXPRESSION IN BRAIN TUMORS

2021 ◽  
Vol 3 (Supplement_1) ◽  
pp. i2-i2
Author(s):  
Georgios Batsios ◽  
Celine Taglang ◽  
Meryssa Tran ◽  
Anne Marie Gillespie ◽  
Joseph Costello ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
In Vivo Imaging ◽  
Lactate Production ◽  
Telomere Maintenance ◽  
Resonance Spectroscopy ◽  
Low Grade ◽  
Non Invasive ◽  
Tert Expression

Abstract Telomere shortening constitutes a natural barrier to uncontrolled proliferation and all tumors must find a mechanism of maintaining telomere length. Most human tumors, including high-grade primary glioblastomas (GBMs) and low-grade oligodendrogliomas (LGOGs) achieve telomere maintenance via reactivation of the expression of telomerase reverse transcriptase (TERT), which is silenced in normal somatic cells. TERT expression is, therefore, a driver of tumor proliferation and, due to this essential role, TERT is also a therapeutic target. However, non-invasive methods of imaging TERT are lacking. The goal of this study was to identify magnetic resonance spectroscopy (MRS)-detectable metabolic biomarkers of TERT expression that will enable non-invasive visualization of tumor burden in LGOGs and GBMs. First, we silenced TERT expression by RNA interference in patient-derived LGOG (SF10417, BT88) and GBM (GS2) models. Our results linked TERT silencing to significant reductions in steady-state levels of NADH in all models. NADH is essential for the conversion of pyruvate to lactate, suggesting that measuring pyruvate flux to lactate could be useful for imaging TERT status. Recently, deuterium (2H)-MRS has emerged as a novel, clinically translatable method of monitoring metabolic fluxes in vivo. However, to date, studies have solely examined 2H-glucose and the use of [U-2H]pyruvate for non-invasive 2H-MRS has not been tested. Following intravenous injection of a bolus of [U-2H]pyruvate, lactate production was higher in mice bearing orthotopic LGOG (BT88 and SF10417) and GBM (GS2) tumor xenografts relative to tumor-free mice, suggesting that [U-2H]pyruvate has the potential to monitor TERT expression in vivo. In summary, our study, for the first time, shows the feasibility and utility of [U-2H]pyruvate for in vivo imaging. Importantly, since 2H-MRS can be implemented on clinical scanners, our results provide a novel, non-invasive method of integrating information regarding a fundamental cancer hallmark, i.e. TERT, into glioma patient management.

Sign in / Sign up

Export Citation Format

Share Document