Study on the mechanism of microporous sheep ADM combined with hUCMSC in promoting wound healing

Objective: To explore the therapeutic mechanism of microporous sheep acellular dermal matrix (ADM) combined with human umbilical cord mesenchymal stem cell (hUCMSC) to promote the healing of full-thickness skin defect.Methods: hUCMSC was co-cultured on microporous sheep ADM to form composite biological dressings. Seventy-two nude mice were selected to make full-thickness skin injury models and randomly divided into 3 groups (hUCMSC + microporous sheep ADM group, sheep ADM group and iodophor gauze group), with 24 mice in each group. The wound healing rate of each group was detected at 14 d, 21 d and 28 d after operation, qRT-PCR technique was used to detect the expression of Bax and Bcl-2 in the wound tissues, and the immunohistochemical staining technique was used to detect the expression of Collagen I and vascular endothelial growth factor (VEGF). Data were analyzed with one-way ANOVA and t test.Results: At 14 d after operation, the wound healing rate of the hUCMSC + microporous sheep ADM group was (65.34 ± 14.72)%, which was significantly higher than that of the iodophor gauze group [(37.54 ± 10.21)%], and higher than that of the sheep ADM group [(49.08 ± 11.16)%], the differences were statistically significant (t = 19.52, 14.72; p < .05). With the gradual healing of the wound, at 28 d after operation, the wound healing rate of the hUCMSC + microporous sheep ADM group was (98.63 ± 15.41)%, which was significantly higher than that of the iodophor gauze group [(81.74 ± 16.27)%], and higher than that of the sheep ADM group [(63.47 ± 14.80)%], the differences were statistically significant (t = -16.42, 20.35; p < .05). The expression of Bax in the wound tissues of the hUCMSC + microporous sheep ADM group was significantly reduced, especially at 21 d after operation, the expression level was 0.25 ± 0.06, which was significantly lower than the iodophor gauze group (0.53 ± 0.16) and the sheep ADM group (0.41 ± 0.12), the differences were statistically significant (t = 3.52, -2.83; p < .05). The expression of Bcl-2 in the wound tissues of the hUCMSC + microporous sheep ADM group was significantly higher than those ofthe other two groups, especially at 21 d after operation, the expression level was 0.63 ± 0.19, which was significantly higher than the iodophor gauze group (0.34 ± 0.09) and the sheep ADM group (0.46 ± 0.13), the differences were statistically significant (t = 5.31, -6.07; p < .05). Immunohistochemical detection showed that the expression of Collagen I and VEGF in the hUCMSC + microporous sheep ADM group was slightly more than that of the sheep ADM group and the iodophor gauze group, but the effect was not remarkable.Conclusions: hUCMSC + microporous sheep ADM composite dressing can promote the healing of the full-thickness skin injury and reduce the production of apoptotic cells by carrying hUCMSC.

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One-step approach for full-thickness skin defect reconstruction in rats using minced split-thickness skin grafts with Pelnac overlay

Burns & Trauma ◽

10.1186/s41038-019-0157-0 ◽

2019 ◽

Vol 7 ◽

Cited By ~ 2

Author(s):

Tong Liu ◽

Chao Qiu ◽

Chi Ben ◽

Haihang Li ◽

Shihui Zhu

Keyword(s):

Wound Healing ◽

Healing Rate ◽

Skin Grafting ◽

Skin Defect ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin ◽

Split Thickness Skin Grafting

Abstract Background Split-thickness skin grafting is the current gold standard for the treatment of traumatic skin loss. However, for patients with extensive burns, split-thickness skin grafting is limited by donor skin availability. Grafting split-thickness skin minced into micrografts increases the expansion ratio but may reduce wound repair quality. Dermal substitutes such as Pelnac can enhance the healing of full-thickness skin wounds, but their application currently requires two surgeries. The present study investigated whether it is possible to repair full-thickness skin defects and improve wound healing quality in a single surgery using Pelnac as an overlay of minced split-thickness skin grafts in a rat model. Methods A full-thickness skin defect model was established using male Sprague-Dawley rats of 10 weeks old. The animals were randomly divided into control and experimental groups in which Vaseline gauze and Pelnac, respectively, were overlaid on minced split-thickness skin grafts to repair the defects. Wound healing rate and quality were compared between the two groups. For better illustration of the quality of wound healing, some results were compared with those obtained for normal skin of rats. Results We found that using Pelnac as an overlay for minced split-thickness skin grafts accelerated wound closure and stimulated cell proliferation and tissue angiogenesis. In addition, this approach enhanced collagen synthesis and increased the formation of basement membrane and dermis as well as the expression of growth factors related to wound healing while reducing scar formation. Conclusions Using minced split-thickness skin grafts overlaid with Pelnac enables the reconstruction of full-thickness skin defects in a single step and can increase the healing rate while improving the quality of wound healing.

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Sericin hydrogels promote skin wound healing with effective regeneration of hair follicles and sebaceous glands after complete loss of epidermis and dermis

Biomaterials Science ◽

10.1039/c8bm00934a ◽

2018 ◽

Vol 6 (11) ◽

pp. 2859-2870 ◽

Cited By ~ 24

Author(s):

Chao Qi ◽

Luming Xu ◽

Yan Deng ◽

Guobin Wang ◽

Zheng Wang ◽

...

Keyword(s):

Wound Healing ◽

Skin Wound ◽

Hair Follicles ◽

Complete Loss ◽

Full Thickness ◽

Sebaceous Glands ◽

Skin Injury ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin

Treating full-thickness skin injury with photo-crosslinkable sericin hydrogel for scarless regeneration with effective restoration of skin appendages.

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Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full‐Thickness Skin Regeneration During Wound Healing

Small ◽

10.1002/smll.201900046 ◽

2019 ◽

Vol 15 (12) ◽

pp. 1900046 ◽

Cited By ~ 198

Author(s):

Yongping Liang ◽

Xin Zhao ◽

Tianli Hu ◽

Baojun Chen ◽

Zhanhai Yin ◽

...

Keyword(s):

Wound Healing ◽

Antibacterial Activity ◽

Drug Release ◽

Skin Regeneration ◽

Full Thickness ◽

Sustained Drug Release ◽

Composite Hydrogels ◽

Full Thickness Skin ◽

Thickness Skin

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Assessment of wound healing activity of ethanolic extracts of Pistacia lentiscus L. leaves and Quercus ilex L. bark in full thickness skin excision in rats

Advances in Traditional Medicine ◽

10.1007/s13596-021-00557-8 ◽

2021 ◽

Author(s):

Houari Hemida ◽

Koula Doukani ◽

Abdelkader Zitouni ◽

Brahim Miloud ◽

Houcine Beggar ◽

...

Keyword(s):

Wound Healing ◽

Quercus Ilex ◽

Pistacia Lentiscus ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin ◽

Ethanolic Extracts ◽

Skin Excision ◽

Wound Healing Activity

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The effect of Korean Red Ginseng on full-thickness skin wound healing in rats

Journal of Ginseng Research ◽

10.1016/j.jgr.2017.12.006 ◽

2019 ◽

Vol 43 (2) ◽

pp. 226-235 ◽

Cited By ~ 6

Author(s):

Ki-Soo Park ◽

Dae-Hwan Park

Keyword(s):

Wound Healing ◽

Skin Wound ◽

Full Thickness ◽

Red Ginseng ◽

Korean Red Ginseng ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin

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Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

Cell Transplantation ◽

10.1177/0963689718797570 ◽

2018 ◽

Vol 27 (10) ◽

pp. 1535-1547 ◽

Cited By ~ 7

Author(s):

Niann-Tzyy Dai ◽

Wen-Shyan Huang ◽

Fang-Wei Chang ◽

Lin-Gwei Wei ◽

Tai-Chun Huang ◽

...

Keyword(s):

Wound Healing ◽

Human Plasma ◽

Three Dimensional ◽

Skin Substitute ◽

Strength Analysis ◽

Full Thickness ◽

Skin Substitutes ◽

Full Thickness Skin ◽

Post Surgery ◽

Thickness Skin

Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay. Experimental studies in mouse full-thickness skin wound models showed that the pre-vascularized gel scaffold significantly accelerated wound healing 7 days after surgery, and resembled normal skin structures after 14 days post-surgery. Histological analysis revealed that pre-vascularized gel scaffolds were well integrated in the host skin, resulting in the vascularization of both the epidermis and dermis in the wound area. Moreover, mechanical strength analysis demonstrated that the healed wound following the implantation of the pre-vascularized gel scaffolds exhibited good tensile strength. Taken together, this novel pre-vascularized human plasma gel scaffold has great potential in skin tissue engineering.

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