scholarly journals Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection

2020 ◽  
Vol 41 (1) ◽  
pp. 199
Author(s):  
Taiane Acunha Escobar ◽  
Gabriela Döwich ◽  
Letícia Carvalho Cantele ◽  
Geórgia Camargo Góss ◽  
Marcelo Lameiro Porciúncula ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Leishmania Species ◽  
Leishmania Infection ◽  
Chain Reaction ◽  
Endemic Region ◽  
Gene Markers ◽  
Horse Blood ◽  
Polymerase Chain

Leishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.

scholarly journals USE OF THE POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF ASYMPTOMATIC Leishmania INFECTION IN A VISCERAL LEISHMANIASIS-ENDEMIC AREA

2013 ◽  
Vol 55 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Luciana Almeida Silva ◽  
Héctor Dardo Romero ◽  
Aline Fagundes ◽  
Nédia Nehme ◽  
Otávio Fernandes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Endemic Area ◽  
Asymptomatic Infection ◽  
Control Measures ◽  
Skin Tests ◽  
Leishmania Infection ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain

The diagnosis of asymptomatic infection with Leishmania (Leishmania) chagasi has become more important over recent years. Expansion of visceral leishmaniasis might be associated with other routes of transmission such as transfusion, congenital or even vector transmission, and subjects with asymptomatic infection are potential reservoirs. Moreover, the identification of infection may contribute to the management of patients with immunosuppressive conditions (HIV, transplants, use of immunomodulators) and to the assessment of the effectiveness of control measures. In this study, 149 subjects living in a visceral leishmaniasis endemic area were evaluated clinically and submitted to genus-specific polymerase chain reaction (PCR), serological testing, and the Montenegro skin test. Forty-nine (32.9%) of the subjects had a positive PCR result and none of them developed the disease within a follow-up period of three years. No association was observed between the results of PCR, serological and skin tests. A positive PCR result in subjects from the endemic area did not indicate a risk of progression to visceral leishmaniasis and was not associated with a positive result in the serological tests.

scholarly journals Validity of the polymerase chain reaction in the diagnosis of clinically suspected cases of American visceral leishmaniasis

2013 ◽  
Vol 17 (3) ◽  
pp. 319-323
Author(s):  
Celia Maria Silva Pedrosa ◽  
Ricardo Arraes de Alencar Ximenes ◽  
Wendell Alexandre Pinheiro de Almeida ◽  
Eliana Maria Mauricio da Rocha
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Chain Reaction ◽  
Polymerase Chain

Case Report: Progressive Dysphonia and Dysphagia due to Laryngeal Leishmaniasis

2021 ◽  
Author(s):  
Laura Renard ◽  
Adrien Lemaignen ◽  
Guillaume Desoubeaux ◽  
David Bakhos
Keyword(s):  
Polymerase Chain Reaction ◽  
Weight Loss ◽  
Case Report ◽  
Amphotericin B ◽  
Laryngeal Cancer ◽  
Complete Resolution ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Histopathology Examination

Laryngeal leishmaniasis is an unusual form of the disease. We report the case of a patient who consulted for dysphonia and dysphagia in a context of asthenia and weight loss. The patient had lesions that were suggestive of laryngeal cancer but were revealed to be leishmaniasis by histopathology examination and polymerase chain reaction. Treatment with amphotericin B and miltefosine permitted complete resolution of the lesions and no recurrence during the 18-month follow-up period.

Polymerase chain reaction-based identification of New World Leishmania species complexes by specific kDNA probes

Acta Tropica ◽  
1999 ◽  
Vol 73 (3) ◽  
pp. 283-293 ◽  
Author(s):  
S.F Brenière ◽  
J Telleria ◽  
M.F Bosseno ◽  
R Buitrago ◽  
B Bastrenta ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
New World ◽  
Leishmania Species ◽  
Chain Reaction ◽  
Species Complexes ◽  
Polymerase Chain
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