Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system

Animal waste is widely used in organic production systems. However, these residues can increase antimicrobial determinants in the soil. In this perspective, this study was developed to evaluate the presence of sulfonamide resistance genes in soils from an organic production system that received animal waste as organic fertilizer. Soil samples were collected from four properties with different management practices to increase soil fertility. Three properties use the animal waste from the conventional system and the other use plant residues as soil cover and a legal reserve. The extraction of total DNA from soil was carried out followed by the amplification of genes encoding sulfonamide resistance (sul1 and sul2) by the PCR (polymerase chain reaction) technique. The sul1 and sul2 genes were detected only in soils treated with animal waste. The genes were not detected in soils from the legal reserve and the property that used plant residues as soil cover. These results indicate that the use of animal waste as agricultural fertilizer can increase genes for resistance to antimicrobials in the soil and the composting process may not be enough to eliminate them. This information reiterates the need to implement standards that establish quality parameters for animal waste, considering resistance to antimicrobials, as well as the development of management strategies that reduce the risk of spreading resistance to antimicrobials when these residues are applied to soils.

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Distribution of Sulfonamide Resistance Genes in Escherichia coli and Salmonella Isolates from Swine and Chickens at Abattoirs in Ontario and Québec, Canada

Applied and Environmental Microbiology ◽

10.1128/aem.02844-08 ◽

2009 ◽

Vol 75 (18) ◽

pp. 5999-6001 ◽

Cited By ~ 34

Author(s):

Gosia K. Kozak ◽

David L. Pearl ◽

Julia Parkman ◽

Richard J. Reid-Smith ◽

Anne Deckert ◽

...

Keyword(s):

Escherichia Coli ◽

Resistance Genes ◽

E Coli ◽

Significant Difference ◽

Sulfonamide Resistance ◽

Sulfonamide Resistance Genes

ABSTRACT Sulfonamide-resistant Escherichia coli and Salmonella isolates from pigs and chickens in Ontario and Québec were screened for sul1, sul2, and sul3 by PCR. Each sul gene was distributed differently across populations, with a significant difference between distribution in commensal E. coli and Salmonella isolates and sul3 restricted mainly to porcine E. coli isolates.

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Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs, pig carcasses and human

Acta Veterinaria Scandinavica ◽

10.1186/1751-0147-52-47 ◽

2010 ◽

Vol 52 (1) ◽

pp. 47 ◽

Cited By ~ 50

Author(s):

Shuyu Wu ◽

Anders Dalsgaard ◽

Anette M Hammerum ◽

Lone J Porsbo ◽

Lars B Jensen

Keyword(s):

Escherichia Coli ◽

Resistance Genes ◽

Sulfonamide Resistance ◽

Pig Carcasses ◽

Sulfonamide Resistance Genes

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Economics of Organic Vs Inorganic Carrot Production in Nepal

Journal of Agriculture and Environment ◽

10.3126/aej.v10i0.2127 ◽

2009 ◽

Vol 10 ◽

pp. 27-33 ◽

Cited By ~ 5

Author(s):

Raj Kumar Adhikari

Keyword(s):

Production System ◽

Organic Fertilizer ◽

Unit Cost ◽

Organic Production ◽

Cost Ratio ◽

Gross Margin ◽

Benefit Cost Ratio ◽

Tillage Operation ◽

The Cost ◽

Benefit Cost

During February-April 2008, a study was conducted to compare the yield and economics of organic and inorganic carrot production and its profit volume in Chitwan district of Nepal. Face to face interview method was used to collect the primary information from randomly selected organic and inorganic carrot producers. Among the cost components, per unit cost on female labor and organic fertilizer were found to be higher in organic production system where as higher per unit cost on seed, tillage operation and male labor were found in inorganic production system. Higher cost and higher revenue was found in inorganic production system but higher benefit cost ratio was found in organic production system. This revealed that adoption of organic carrot production system was economically profitable than inorganic production system. Key words: Benefit cost ratio; Carrot; Cost; Gross margin; Organic agricultureThe Journal of Agriculture and Environment Vol:10, Jun.2009 Page: 27-33

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Occurrence of selected PPCPs and sulfonamide resistance genes associated with heavy metals pollution in surface sediments from Chao Lake, China

Environmental Earth Sciences ◽

10.1007/s12665-015-4838-0 ◽

2015 ◽

Vol 75 (1) ◽

Cited By ~ 8

Author(s):

Yang Wu ◽

Chang-ping Yu ◽

Mei Yue ◽

Sheng-ping Liu ◽

Xiao-yong Yang

Keyword(s):

Heavy Metals ◽

Resistance Genes ◽

Surface Sediments ◽

Metals Pollution ◽

Sulfonamide Resistance ◽

Heavy Metals Pollution ◽

Sulfonamide Resistance Genes

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Mobilome-driven segregation of the resistome in biological wastewater treatment

10.1101/2021.11.15.468621 ◽

2021 ◽

Author(s):

Laura de Nies ◽

Susheel Bhanu Busi ◽

Benoit Josef Kunath ◽

Patrick May ◽

Paul Wilmes

Keyword(s):

Wastewater Treatment ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Essential Elements ◽

Biological Wastewater Treatment ◽

Longitudinal Assessment ◽

Aminoglycoside Resistance ◽

Antimicrobial Resistance Genes ◽

Sulfonamide Resistance ◽

Sulfonamide Resistance Genes

Biological wastewater treatment plants (BWWTP) are considered to be hotspots of evolution and subsequent spread of antimicrobial resistance (AMR). Mobile genetic elements (MGEs) promote the mobilization and dissemination of antimicrobial resistance genes (ARGs) and are thereby critical mediators of AMR within the BWWTP microbial community. At present, it is unclear whether specific AMR categories are differentially disseminated via bacteriophages (phages) or plasmids. To understand the segregation of AMR in relation to MGEs, we analyzed meta-omic (metagenomic, metatranscriptomic and metaproteomic) data systematically collected over 1.5 years from a BWWTP. Our results showed a core group of fifteen AMR categories which were found across all timepoints. Some of these AMR categories were disseminated exclusively (bacitracin) or primarily (aminoglycoside, MLS, sulfonamide) via plasmids or phages (fosfomycin and peptide), whereas others were disseminated equally by both MGEs. Subsequent expression- and protein-level analyses further demonstrated that aminoglycoside, bacitracin and sulfonamide resistance genes were expressed more by plasmids, in contrast to fosfomycin and peptide AMR expression by phages, thereby validating our genomic findings. Longitudinal assessment further underlined these findings whereby the log2-fold changes of aminoglycoside, bacitracin and sulfonamide resistance genes were increased in plasmids, while fosfomycin and peptide resistance showed similar trends in phages. In the analyzed communities, the dominant taxon Candidatus Microthrix parvicella was a major contributor to several AMR categories whereby its plasmids primarily mediated aminoglycoside resistance. Importantly, we also found AMR associated with ESKAPEE pathogens within the BWWTP, for which MGEs also contributed differentially to the dissemination of ARGs. Collectively our findings pave the way towards understanding the segmentation of AMR within MGEs, thereby shedding new light on resistome populations and their mediators, essential elements that are of immediate relevance to human health.

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