The importance of C-reactive protein levels for surgical decision-making and determination of response to conservative therapy in patients with acute sciatica

C-reactive protein (CRP), a humoral component of the innate immune system with important functions in host-defense, is extensively used as a sensitive biomarker of systemic inflammation. During inflammation, hepatocyte-derived CRP rises dramatically in the blood due to increased interleukin-6 (IL-6) levels. Reliable detection of CRP in saliva, instead of blood, would offer advantages regarding sampling procedure and availability but using saliva as a diagnostic body fluid comes with challenges. The aims of this study were to evaluate associations between salivary CRP, total protein levels in saliva and serum CRP. Furthermore, we examined associations with plasma IL-6, body mass index (BMI), tobacco smoking and age. Salivary CRP was investigated by ELISA in 107 middle-aged participants from the general population. We employed spectrophotometric determination of total protein levels. Correlation analyses were used for associations of salivary CRP with serum CRP (turbidimetry), plasma IL-6 (Luminex®), BMI and smoking habits. Salivary median CRP was 68% higher (p=0.009), and total protein levels were 167% higher (p<0.0001), in morning compared to evening saliva. The correlation coefficients between serum and salivary CRP were low to moderate, but stronger for evening than morning saliva. Plasma IL-6 correlated significantly with serum CRP (rs=0.41, p<0.01), but not with morning or evening salivary CRP. Non-smokers showed 103% higher salivary CRP levels (p=0.015), whereas serum CRP was independent of smoking status. As opposed to CRP in serum, salivary CRP was not associated with BMI. Salivary CRP was 90% higher among the age interval 60–69 years compared to subjects aged 45–59 (p=0.02) while serum CRP levels did not differ between the age groups. In conclusion, CRP in saliva did not straightforwardly reflect serum concentrations. This raises questions regarding adequate reflection of biological events. The pronounced diurnal salivary CRP pattern accentuates the importance of standardizing the time-point of sampling.

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Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study

Bioanalysis ◽

10.4155/bio.14.12 ◽

2014 ◽

Vol 6 (11) ◽

pp. 1459-1470 ◽

Cited By ~ 12

Author(s):

Celine IL Justino ◽

Kátia Duarte ◽

Susana Lucas ◽

Paulo Chaves ◽

Paulo Bettencourt ◽

...

Keyword(s):

Cardiovascular Disease ◽

Pilot Study ◽

Disease Risk ◽

Cardiovascular Disease Risk ◽

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein

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Surgeons’ Re-Operative Valve Replacement Practices with Patients with Endocarditis Due to Drug Use

10.20944/preprints202008.0531.v1 ◽

2020 ◽

Author(s):

Julie Aultman ◽

Oliwier Dziadkowiec ◽

Dianne McCallister ◽

Michael Firstenberg

Keyword(s):

Decision Making ◽

Drug Use ◽

Valve Replacement ◽

Heart Valves ◽

Intravenous Drug Use ◽

Surgical Decision ◽

Attitudes And Practices ◽

Survey Results ◽

Surgical Decision Making

Background: This study discerns surgeons’ attitudes and practices in the determination of heart valve replacement for patients with endocarditis due to intravenous drug use (IVDU-IE). We aimed to identify factors contributing to surgeons’ decision-making process for initial and recurrent surgical heart valves, and the availability of institutional guidance. Methods: An IRB approved, anonymous mixed-methods survey instrument was designed and validated with 24 questions. Cardiothoracic surgeons in the U.S. and globally were recruited with a total of 220 enrolling in the study with 176 completing every question on the survey. Results: A cluster analysis revealed that although surgeons can be divided into sub-groups based on their previous experience with valve replacements, these groups are not perfectly homogenous, and the number of identified clusters is dependent on technique used. ANOVA analysis revealed that the variables that most clearly divided the surgeons into subgroups were, in order of importance, years of practice, number of valve replacements, and geography. Conclusions: Our analysis showed heterogeneity among cardiothoracic surgeons regarding how they make clinical decisions regarding re-operative valve replacement related to IVDU-IE Therefore, an opportunity exists for an interprofessional team to develop guidelines to decrease variability in surgical decision-making regarding valve replacement associated with IVDU-IE

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Immunoassay methods for determination of serum C-reactive protein levels

La Ricerca in Clinica e in Laboratorio ◽

10.1007/bf02904892 ◽

1984 ◽

Vol 14 (3) ◽

pp. 581-585

Author(s):

Giampaolo Merlini ◽

Franco Pavesi ◽

Cesare Bergonzi ◽

Vittorio Bellotti ◽

Irene Zorzoli ◽

...

Keyword(s):

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein

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1228: Assessing the Surgical Decision-Making Ability of Novice and Proficient Urologists

The Journal of Urology ◽

10.1016/s0022-5347(18)31442-3 ◽

2007 ◽

Vol 177 (4S) ◽

pp. 405-405

Author(s):

Suman Chatterjee ◽

Jonathon Ng ◽

Edward D. Matsumoto

Keyword(s):

Decision Making ◽

Surgical Decision ◽

Surgical Decision Making

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Positive correlation between neovascularization degree of carotid atherosclerosis determined by contrast-enhanced ultrasound and level of serum C-reactive protein

VASA ◽

10.1024/0301-1526/a000429 ◽

2015 ◽

Vol 44 (3) ◽

pp. 0187-0194 ◽

Cited By ~ 6

Author(s):

Xiaoni Chang ◽

Jun Feng ◽

Litao Ruan ◽

Jing Shang ◽

Yanqiu Yang ◽

...

Keyword(s):

Contrast Enhancement ◽

Rank Correlation ◽

Artery Stenosis ◽

Contrast Enhanced Ultrasound ◽

C Reactive Protein ◽

Protein Levels ◽

Reactive Protein ◽

Contrast Enhanced ◽

Grade 3 ◽

Axis View

Background: Neovascularization is one of the most important risk factors for unstable plaque. This study was designed to correlate plaque thickness, artery stenosis and levels of serum C-reactive protein with the degree of intraplaque enhancement determined by contrast-enhanced ultrasound. Patients and methods: Contrast-enhanced ultrasound was performed on 72 carotid atherosclerotic plaques in 48 patients. Contrast enhancement within the plaque was categorized as grade 1, 2 or 3. Maximum plaque thickness was measured in short-axis view. Carotid artery stenosis was categorized as mild, moderate or severe. Results: Plaque contrast enhancement was not associated with the degree of artery stenosis or with plaque thickness. Serum C-reactive protein levels were positively correlated with the number of new vessels in the plaque. C-reactive protein levels increased in the three groups(Grade 1: 3.72±1.79mg/L; Grade 2: 7.88±4.24 mg/L; Grade 3: 11.02±3.52 mg/L), with significant differences among them (F=10.14, P<0.01), and significant differences between each two groups (P<0.05). Spearmans rank correlation analysis showed that serum C-reactive protein levels were positively correlated with the degree of carotid plaque enhancement (Rs =0.69, P<0.01). Conclusions: The combination of C-reactive protein levels and intraplaque neovascularization detected by contrast-enhanced ultrasound may allow more accurate evaluation of plaque stability.

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