In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates

The chemical composition of the essential oils of Achillea holosericea, Achillea taygetea, Achillea fraasii was determined by GC/MS analysis. Among the ninety-five assayed constituents, camphor, borneol and 1,8-cineol were found to be the major components. The in-vitro antimicrobial activity of these essential oils was evaluated against six bacteria indicating that the first is totally inactive, while the other two possess moderate to strong activities mainly against the Gram negative strains. The essential oil of A. fraasii was also active against the tested pathogenic fungi

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In-vitro antimicrobial activity of HSR-903, a new fluoroquinolone, against clinical isolates of Neisseria gonorrhoeae with quinolone resistance-associated alterations in GyrA and ParC

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/40.3.437 ◽

1997 ◽

Vol 40 (3) ◽

pp. 437-439 ◽

Cited By ~ 5

Author(s):

T Deguchi

Keyword(s):

Antimicrobial Activity ◽

Neisseria Gonorrhoeae ◽

Quinolone Resistance ◽

Clinical Isolates ◽

In Vitro Antimicrobial Activity

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In vitro Antimicrobial Activity of Gatifloxacin Compared with Other Quinolones against Clinical Isolates from Cancer Patients

Chemotherapy ◽

10.1159/000081708 ◽

2004 ◽

Vol 50 (5) ◽

pp. 214-220 ◽

Cited By ~ 10

Author(s):

Kenneth V.I. Rolston ◽

Irfan Vaziri ◽

Susan Frisbee-Hume ◽

Harriet Streeter ◽

Barbara LeBlanc

Keyword(s):

Antimicrobial Activity ◽

Cancer Patients ◽

Clinical Isolates ◽

In Vitro Antimicrobial Activity

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Volatile Oils ofNepeta tenuifolia(Jing Jie) as an Alternative Medicine against Multidrug-Resistant Pathogenic Microbes

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2018/8347403 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Yi-Hsuan Lee ◽

Chao-Min Wang ◽

Po-Yu Liu ◽

Ching-Chang Cheng ◽

Zong-Yen Wu ◽

...

Keyword(s):

Essential Oils ◽

In Vitro Study ◽

Multidrug Resistant ◽

Clinical Isolates ◽

Gram Negative ◽

E Coli ◽

Wide Range ◽

Main Component ◽

Reference Strains

Essential oils from the dried spikes ofNepeta tenuifolia(Benth) are obtained by steam distillation. Pulegone was identified as the main component in the spikes ofN. tenuifoliathrough analysis, with greater than 85% purity obtained in this study. The essential oils are extremely active against all Gram-positive and some Gram-negative reference bacteria, particularlySalmonella enterica,Citrobacter freundii, andEscherichia coli. The minimum inhibitory concentration was found to be between 0.08 and 0.78% (againstS. enterica), 0.39 and 0.78% (againstC. freundii), and 0.097 and 0.39% (againstE. coli), whereas the minimum bactericidal concentration varied in range from 0.097% to 1.04%. In general, the essential oils show a strong inhibitory action against all tested reference strains and clinical isolates. However, the antibacterial activity of EOs against bothPseudomonas aeruginosareference strains and clinical isolates was relatively lower than other Gram-negative pathogens. The essential oils ofN. tenuifoliaalso displayed bactericidal activities (MBC/MIC < 4) in this study. These findings reflect the bactericidal activity of the essential oils against a wide range of multidrug-resistant clinical pathogens in an in vitro study. In addition, we propose the fragmentation pathways of pulegone and its derivatives by LC-ESI-MS/MS in this study.

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Chemical Composition and in vitro Antimicrobial Activity of the Essential Oils of Two Helichrysum Species from Tanzania

Zeitschrift für Naturforschung C ◽

10.1515/znc-2004-5-614 ◽

2004 ◽

Vol 59 (5-6) ◽

pp. 368-372 ◽

Cited By ~ 52

Author(s):

Christos Bougatsos ◽

Olipa Ngassapa ◽

Deborah K. B. Runyoro ◽

Ioanna B. Chinou

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Pathogenic Fungi ◽

Caryophyllene Oxide ◽

In Vitro Antimicrobial Activity ◽

Aerial Parts ◽

Main Components

The chemical composition of the essential oils obtained from the aerial parts of Helichrysum cymosum and H. fulgidum, from Tanzania, were analyzed by GC and GC/MS. A total of sixty-five compounds, representing 92.4% and 88.2% of the two oils, respectively, were identified. trans-Caryophyllene, caryophyllene oxide, β-pinene, p-cymene, spathulenol and β- bourbonene were found to be the main components. Furthermore, the oils were tested against six gram (±) bacteria and three pathogenic fungi. It was found that the oil of H. fulgidum exhibited significant antimicrobial activity, while the oil of H. cymosum was not active at all.

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