scholarly journals Karakteristik Fitokimia dan Aktivitas Antioksidan Ekstrak Pupa Black Soldier Fly (BSF)

WARTA AKAB ◽  
2021 ◽  
Vol 45 (2) ◽  
Author(s):  
Tri Sutanti Budikania ◽  
Herawati Herawati ◽  
Amalia Fitrian Nasution
Keyword(s):  
Radical Scavenger ◽  
Black Soldier Fly

The Black Soldier Fly (BSF) merupakan spesies lalat daerah tropis yang dapat menguraikan senyawa organik, sehingga banyak dimanfaatkan untuk mengatasi limbah organic di perkotaan. Larva BSF memiliki kemampuan mengkonsumsi limbah organik dalam jumlah besar dan cepat karena bagian mulut dan enzim pencernaan yang aktif. Larva BSF juga dimanfaatkan sebagai pakan ternak karena memiliki kadar protein dan lemak yang tinggi. Pupa BSF kaya akan senyawa kitin dan digunakan untuk bahan baku kitosan. Pupa BSF diduga mengandung senyawa metabolit sekunder yang memiliki sifat antioksidan. Tujuan penelitian adalah untuk mengetahui kandungan senyawa metabolit sekunder serta sifat aktivitas antioksidannya. Pengujian senyawa metabolit sekunder menggunakan skrining fitokimia sedangkan aktivitas antioksidan menggunakan metode radical scavenger. Hasil penelitian menunjukkan bahwa ekstrak heksana mengandung alkaloid, flavonoid, saponin, terpenoid dan triterpenoid. Ekstrak etil asetat mengandung alkaloid, saponin dan terpenoid, sedangkan  ekstrak metanol mengandung alkaloid, saponin dan terpenoid. Nilai IC50 pada larutan ekstrak n-heksan, etil asetat dan metanol berturut-turut adalah 41,18; 36,92 dan 17,45 mg/L. Nilai IC50 ini menunjukkan bahwa pupa BSF memiliki sifat antioksidan sangat kuat. Kata Kunci : BSF; fitokimia; antioksidan

Melatonin reduces high levels of lipid peroxidation induced by potassium iodate in porcine thyroid

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Paulina Iwan ◽  
Jan Stepniak ◽  
Malgorzata Karbownik-Lewinska
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Membrane Lipids ◽  
Chemical Properties ◽  
Iodine Concentration ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Protective Effects ◽  
Potassium Iodate ◽  
Hormone Synthesis

Abstract. Iodine is essential for thyroid hormone synthesis. Under normal iodine supply, calculated physiological iodine concentration in the thyroid is approx. 9 mM. Either potassium iodide (KI) or potassium iodate (KIO3) are used in iodine prophylaxis. KI is confirmed as absolutely safe. KIO3 possesses chemical properties suggesting its potential toxicity. Melatonin (N-acetyl-5-methoxytryptamine) is an effective antioxidant and free radical scavenger. Study aims: to evaluate potential protective effects of melatonin against oxidative damage to membrane lipids (lipid peroxidation, LPO) induced by KI or KIO3 in porcine thyroid. Homogenates of twenty four (24) thyroids were incubated in presence of either KI or KIO3 without/with melatonin (5 mM). As melatonin was not effective against KI-induced LPO, in the next step only KIO3 was used. Homogenates were incubated in presence of KIO3 (200; 100; 50; 25; 20; 15; 10; 7.5; 5.0; 2.5; 1.25 mM) without/with melatonin or 17ß-estradiol. Five experiments were performed with different concentrations of melatonin (5.0; 2.5; 1.25; 1.0; 0.625 mM) and one with 17ß-estradiol (1.0 mM). Malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration (LPO index) was measured spectrophotometrically. KIO3 increased LPO with the strongest damaging effect (MDA + 4-HDA level: ≈1.28 nmol/mg protein, p < 0.05) revealed at concentrations of around 15 mM, thus corresponding to physiological iodine concentrations in the thyroid. Melatonin reduced LPO (MDA + 4-HDA levels: from ≈0.97 to ≈0,76 and from ≈0,64 to ≈0,49 nmol/mg protein, p < 0.05) induced by KIO3 at concentrations of 10 mM or 7.5 mM. Conclusion: Melatonin can reduce very strong oxidative damage to membrane lipids caused by KIO3 used in doses resulting in physiological iodine concentrations in the thyroid.

OPTIMASI KONDISI EKSTRAKSI SENYAWA AKTIF ANTIOKSIDAN DARI BIJI ANGGUR

2019 ◽  
Vol 3 (2) ◽  
pp. 35
Author(s):  
Kartini Kartini ◽  
Azminah Azminah
Keyword(s):  
Free Radical ◽  
Optimum Condition ◽  
Active Compound ◽  
Grape Seed ◽  
Free Radical Scavenger ◽  
Water Bath ◽  
Radical Scavenger ◽  
Optimum Conditions ◽  
Ethanolic Solution ◽  
Reduced Pressure

In order to prepare standardized extract, optimization of extraction conditions of grape seed has been done. These conditions are type of menstrum (50, 70 and 96% of ethanolic solution), length of extraction (1, 2 and 4 hours) also method of evaporation (reduced pressure and opened air). Activity on free radical scavenger used as parameters to determine optimum conditions. Based on EC50 (concentration which scavenge 50% amount of free radical) can be concluded that optimum condition for extracting antioxidant active compound from grape seed are 70% ethanolic solution as menstrum, length of extraction 1 hour and evaporation on opened air use water bath.

OBTAINING CHITIN FROM THE BLACK SOLDIER FLY HERMETIA ILLUCENS BY DIRECT EXTRACTION

2018 ◽  
Vol 2 (3) ◽  
pp. 84-87 ◽  
Author(s):  
A.S. Khayrova ◽  
◽  
S.A. Lopatin ◽  
O.A. Sinitsyna ◽  
A.P. Sinitsyn ◽  
...  
Keyword(s):  
Direct Extraction ◽  
Black Soldier Fly ◽  
Hermetia Illucens

scholarly journals Impact of black soldier fly larvae meal on the chemical and nutritional characteristics of rainbow trout fillets

animal ◽  
2018 ◽  
Vol 12 (8) ◽  
pp. 1672-1681 ◽  
Author(s):  
S. Mancini ◽  
I. Medina ◽  
V. Iaconisi ◽  
F. Gai ◽  
A. Basto ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Black Soldier Fly ◽  
Nutritional Characteristics ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Metal free decarboxylative aminoxylation of carboxylic acids using a biphasic solvent system

2021 ◽  
Vol 19 (1) ◽  
pp. 273-278
Author(s):  
Göran Schulz ◽  
Andreas Kirschning
Keyword(s):  
Carboxylic Acids ◽  
Solvent System ◽  
Radical Scavenger ◽  
Synthetic Approach ◽  
Metal Free ◽  
System P

The oxidative radical decarboxylation of carboxylic acids with TEMPO as radical scavenger in a biphasic solvent system is reported which is successfully used in a new synthetic approach for the antidepressants indatraline.

Oxygen radicals trigger activation of NF-κB and AP-1 and upregulation of ICAM-1 in reperfused canine heart

2002 ◽  
Vol 282 (5) ◽  
pp. H1778-H1786 ◽  
Author(s):  
Haiying Fan ◽  
Baogui Sun ◽  
Qiuping Gu ◽  
Anne Lafond-Walker ◽  
Suyi Cao ◽  
...  
Keyword(s):  
Dna Binding ◽  
Protein Expression ◽  
Vascular Endothelium ◽  
Oxygen Radicals ◽  
Nuclear Dna ◽  
Ischemia Reperfusion ◽  
Intercellular Adhesion Molecule ◽  
Radical Scavenger ◽  
Myeloperoxidase Activity ◽  
Canine Heart

We investigated whether oxygen radicals generated during ischemia-reperfusion trigger postischemic inflammation in the heart. Closed-chest dogs underwent 90-min coronary artery occlusion, followed by 1- or 3-h reperfusion: 10 dogs received the cell-permeant oxygen radical scavenger N-(2-mercaptopropionyl)-glycine (MPG; 8 mg · kg−1 · h−1intracoronary) beginning 5 min before reperfusion, and 9 dogs received vehicle. Blood flow (microspheres), intercellular adhesion molecule (ICAM)-1 protein expression (immunohistochemistry), ICAM-1 gene activation (Northern blotting), nuclear DNA binding activity of nuclear factor (NF)-κb and AP-1 (electrophoretic mobility shift assays), and neutrophil (PMN) accumulation (myeloperoxidase activity) were assessed in myocardial tissue samples. ICAM-1 protein expression was high in vascular endothelium after ischemia-reperfusion but was markedly reduced by MPG. MPG treatment also markedly decreased expression of ICAM-1 mRNA and tissue PMN accumulation. Nuclear DNA binding activities of NF-κB and AP-1, increased by ischemia-reperfusion, were both markedly decreased by MPG at 1 h of reperfusion. However, by 3 h, AP-1 activity was only modestly reduced by MPG and NF-κB activity was not significantly different from ischemic-reperfused controls. These results suggest that oxygen radicals generated in vivo during reperfusion trigger early activation of NF-κb and AP-1, resulting in upregulation of the ICAM-1 gene in vascular endothelium and subsequent tissue accumulation of activated PMNs.

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