Methanolic Extract of Peel of Citrus maxima Fruits Exhibit Analgesic, CNS Depressant and Anti-inflammatory Activities in Swiss Albino Mice

Background. Ficus hispida is traditionally used in the ailment of pain, inflammation, and neurological disorders. The present study set out to evaluate the in vivo antinociceptive, anti-inflammatory, and sedative activity of the ethanol extract of Ficus hispida bark (EFHB). Methods. The antinociceptive activity of EFHB was evaluated by using acetic acid induced writhing, formalin, hot plate, and tail immersion methods in Swiss albino mice. Its anti-inflammatory activity was assessed by using carrageenan and histamine induced rat paw oedema test in Wister rats. The central stimulating activity was studied by using pentobarbital induced hypnosis, hole cross, and open field tests in Swiss albino mice. Results. EFHB demonstrated antinociceptive activity both centrally and peripherally. It showed 62.24% of writhing inhibition. It significantly inhibited licking responses in early (59.29%) and late phase (71.61%). It increased the reaction time to the thermal stimulus in both hot plate and tail immersion. It inhibited the inflammation to the extent of 59.49%. A substantial increase in duration of sleep up to 60.80 min and decrease of locomotion up to 21.70 at 400 mg/kg were also observed. Conclusion. We found significant dose dependent antinociceptive, anti-inflammatory, and sedative properties of EFHB in experimental animal models.

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Anti inflammatory activity of ethanolic extract of Nymphaea Alba flower in Swiss albino mice

International Journal of Medical Research & Health Sciences ◽

10.5958/j.2319-5886.2.3.082 ◽

2013 ◽

Vol 2 (3) ◽

pp. 474 ◽

Cited By ~ 3

Author(s):

Jacob Jesurun RS ◽

Senthilkumaran Jagadeesh ◽

Somasundaram Ganesan ◽

Venugopala Rao K ◽

Madhavi Eerike

Keyword(s):

Ethanolic Extract ◽

Inflammatory Activity ◽

Swiss Albino Mice ◽

Nymphaea Alba ◽

Albino Mice ◽

Anti Inflammatory

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Hypoglycemic Effect of Aqueous and Methanolic Extract ofArtemisia afraon Alloxan Induced Diabetic Swiss Albino Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/752486 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

Idris Ahmed Issa ◽

Mohammed Hussen Bule

Keyword(s):

Blood Glucose ◽

Aqueous Extract ◽

Methanolic Extract ◽

Addis Ababa ◽

Natural Habitat ◽

Lethal Dose ◽

Pharmaceutical Drugs ◽

Swiss Albino Mice ◽

Antidiabetic Effect ◽

Albino Mice

Diabetes mellitus is metabolic syndrome that causes disability, early death, and many other complications. Currently insulin and many synthetic drugs are used in diabetes treatment. However, these pharmaceutical drugs are too expensive particularly for sub-Saharan population in addition to their undesirable side effects. The present study was aimed to evaluate antidiabetic effect and toxicity level ofArtemisia afrawhich was collected from its natural habitat in Bale Zone, around Goba town, 455 km southeast of Addis Ababa. Air dried aerial parts ofArtemisia afrawere separately extracted with both distilled water and 95% methanol. Oral acute toxicity test was conducted on healthy Swiss albino mice. Antidiabetic effect of the aqueous and methanolic extracts ofArtemisia afrawas separately evaluated on alloxan induced diabetic mice at doses of 500, 750, and 1000 mg/Kg body weight orally. The results indicate that mean lethal dose (LD50) for aqueous extract ofArtemisia afrawas 9833.4 mg/Kg. Blood glucose level was significantly decreased by 24% (p<0.005) and 56.9% (p<0.0004) in groups that received aqueous extract ofArtemisia afraat dose of 500 mg/Kg and 750 mg/Kg, respectively. The methanolic extract ofArtemisia afraalso significantly lowered blood glucose by 49.8% (p<0.0001) at doses of 1000 mg/kg on the 5th hr. Aqueous extract ofArtemisia afrawas regarded as nontoxic and safe since its LD50was found above 5000 mg/Kg. Aqueous extract showed higher effect at relatively lower dose as compared to methanolic extract. The aqueous extract was screened positive for phytochemicals like flavonoids, polyphenols, and tannins that were reported to have antioxidant activity.

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Evaluation of Anti-Inflammatory and Analgesic Activities of Methanolic Extract of Aitchisonia Rosea in Albino Mice

RADS Journal of Pharmacy and Pharmaceutical Sciences ◽

10.37962/jpps.v8i1.371 ◽

2020 ◽

Vol 8 (1) ◽

pp. 31-36

Author(s):

Shahid Rasool ◽

Ambreen Malik Uttra ◽

Mehreen Malik Uttra ◽

Faheem Ahmed ◽

Khizar Abbas

Keyword(s):

Acetic Acid ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Paw Edema ◽

Egg Albumin ◽

Inflammatory Conditions ◽

Albino Mice ◽

Anti Inflammatory

Objective: Current analysis aims at in-vivo investigation of analgesic and anti-inflammatory activities of Aitchisonia rosea methanolic extract. Methods: Albino mice (20-30 g) were used in the study. Extract at 200, 400 and 800 mg/kg doses was tested against egg albumin and carrageenan induced mice paw edema along with formalin persuaded paw licking and acetic acid prompted writhing for analgesic activity. Results: Results depicted dose reliant and significant (p<0.001) inhibition of carrageenan as well as egg albumin brought paw edema, abdominal constrictions/ hind limb stretching tempted by acetic acid and formalin instigated paw licking. Capability of crude extract to halt inflammatory and analgesic response was akin to that of ibuprofen in all models. Conclusion: The current findings support the use of Aitchisonia rosea for inflammatory conditions, which might be attributed to its previously proven high alkaloid, flavonoids, phenol, tannins content and free radical scavenging activity.

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Antinociceptive effect of methanolic extract of Murraya koenigii leaves in swiss albino mice

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20200715 ◽

2020 ◽

Vol 9 (3) ◽

pp. 426

Author(s):

Arunkumar J. ◽

Vijayalakshmi M. ◽

Yesodha S. ◽

YousufAli A. S. ◽

Parthiban R.

Keyword(s):

Reaction Time ◽

Analgesic Activity ◽

Methanolic Extract ◽

Control Group ◽

Murraya Koenigii ◽

Hot Plate ◽

Standard Drug ◽

Swiss Albino Mice ◽

Albino Mice ◽

Tail Clip

Background: The objective of the study was to evaluate anti-nociceptive effect of methanolic extract of Murraya koenigii leaves on thermal and mechanical pain in swiss albino mice.Methods: Thirty adult male swiss albino mice weighing 25-30 grams were selected and allocated in to five groups. Each group consists of six animals. The control group received vehicle (10 ml/kg), standard group received morphine (10 mg/kg) and test groups received dried methanolic extract of Murraya koenigii leaves (100 mg/kg, 200 mg/kg, 400 mg/kg per oral respectively) 1 hour before placing the animal over the hot plate at temperature of 55⁰C . A cut off period of 10 sec was observed to avoid damage of the paw. The response in the form of withdrawal of paws or licking of the paws. The delay in the reaction time denotes analgesic activity. The latency was recorded before and after 15, 30, 60, 120 minutes administration of drug. After washout period of 1 month the same group of animals were utilized to evaluate the analgesic effect by tail clip method for better comparison.Results: All the doses of Murraya koenigii leaves significantly delayed reaction time in hot plate method and tail clip method. The results were comparable to that produced by standard drug morphine.Conclusions: Murraya koenigii leaves has analgesic activity which was comparable to morphine.

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Anxiolytic, antidepressant and anti-inflammatory activities of water-soluble extract of beehive in Swiss albino mice

10.3390/ecmc2020-07254 ◽

2020 ◽

Author(s):

A.S.M. Ali Reza ◽

Talha Bin Emran ◽

Abu Montakim Tareq

Keyword(s):

Water Soluble ◽

Soluble Extract ◽

Swiss Albino Mice ◽

Albino Mice ◽

Anti Inflammatory ◽

Water Soluble Extract

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Anti-Depressant Activity of the Leaves of Zanthoxylum Armatum on Swiss Albino Mice

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v8i02.34389 ◽

2020 ◽

Vol 8 (02) ◽

pp. 46-50

Author(s):

Chandrajeet Kumar Yadav ◽

Kamal Poudel ◽

Roshan Mehta ◽

Amit Kumar Shrivastava

Keyword(s):

Methanolic Extract ◽

Forced Swim Test ◽

Antidepressant Drugs ◽

Antidepressant Activity ◽

Tail Suspension Test ◽

Medical Problem ◽

Mild Stress ◽

Swiss Albino Mice ◽

Zanthoxylum Armatum ◽

Albino Mice

INTRODUCTION Depression is a global mental disorder that has high incidence, high recurrence, and high self-mutilation and suicide rates. Although the antidepressant drugs are available for the treatment, depression still continues to be a major medical problem. The present study was designed to study the anti-depressant activity of the leaves extract of Zanthoxylum armatum using forced swim test and tail suspension test (TST) on Swiss albino mice. MATERIAL AND METHODS The anti-depressant activity of the leaves of Zanthoxylum armatum was assessed using chronic unpredictable mild-stress (CUMS) induced depression in mice. The animals were treated with the methanolic extract of leaves of Zanthoxylum armatum orally at two doses of 100, 200 mg/kg body weight for eight days after (CUMS) induced depression in mice. RESULTS The data were analyzed by one-way ANOVA followed by tukey multiple comparison test. The leaves extract presented significant antidepressant activity in mice (p<0.05), CONCLUSION The results demonstrate that methanolic extract of leaves of Zanthoxylum armatum has got significant antidepressant activity.

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