Ficus hispida Bark Extract Prevents Nociception, Inflammation, and CNS Stimulation in Experimental Animal Model

Background. Ficus hispida is traditionally used in the ailment of pain, inflammation, and neurological disorders. The present study set out to evaluate the in vivo antinociceptive, anti-inflammatory, and sedative activity of the ethanol extract of Ficus hispida bark (EFHB). Methods. The antinociceptive activity of EFHB was evaluated by using acetic acid induced writhing, formalin, hot plate, and tail immersion methods in Swiss albino mice. Its anti-inflammatory activity was assessed by using carrageenan and histamine induced rat paw oedema test in Wister rats. The central stimulating activity was studied by using pentobarbital induced hypnosis, hole cross, and open field tests in Swiss albino mice. Results. EFHB demonstrated antinociceptive activity both centrally and peripherally. It showed 62.24% of writhing inhibition. It significantly inhibited licking responses in early (59.29%) and late phase (71.61%). It increased the reaction time to the thermal stimulus in both hot plate and tail immersion. It inhibited the inflammation to the extent of 59.49%. A substantial increase in duration of sleep up to 60.80 min and decrease of locomotion up to 21.70 at 400 mg/kg were also observed. Conclusion. We found significant dose dependent antinociceptive, anti-inflammatory, and sedative properties of EFHB in experimental animal models.

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Pharmacological Evaluation of Antipyretic, Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Cassia fistula

Indian Journal of Animal Research ◽

10.18805/ijar.b-4531 ◽

2021 ◽

Author(s):

D.K. Sharma ◽

S.K. Sharma ◽

M.K. Lonare ◽

Rajdeep Kaur ◽

V.K. Dumka

Keyword(s):

Analgesic Activity ◽

Ethanolic Extract ◽

Inflammatory Activity ◽

Bark Extract ◽

Control Group ◽

Albino Rats ◽

Hot Plate ◽

Antipyretic Activity ◽

Tail Immersion ◽

Anti Inflammatory

Background: The antipyretic, analgesic and anti-inflammatory activities of two concentrations (100 and 200 mg/kg) of ethanolic extract of leaf, bark, flower and fruit pulp of C. fistula were determined in male wistar albino rats. Methods: Antipyretic activity was assessed by E. coli endotoxin induced pyrexia. Analgesic activity was assessed by hot plate, tail immersion and acetic acid induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced rat paw edema assay. Result: Significant (p less than 0.05) antipyretic activity was exhibited from 2h onwards by bark extract @ 200 mg/kg and from 3h onwards by bark extract @100 mg/kg and leaves extract @ 200mg/kg as compared to control group. Significant (p less than 0.05) analgesic activity was shown by extract of bark @ 200 mg/kg as it is evident by increase in reflex time in hot plate (90,120,180 min), tail immersion test (120,180 min) and inhibition of writhing (32.12%). Significant (p less than 0.05) anti-inflammatory activity was exhibited from 3h post administration by bark @ 200 and leaves @ 100 and 200 mg/kg.

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Atividade antinociceptiva e anti-inflamatória do extrato hexânico de "Echinodorus macrophyllus" (Kunth) Micheli em camundongos

Brazilian Journal of Health and Biomedical Sciences ◽

10.12957/bjhbs.2019.53056 ◽

2019 ◽

Vol 18 (1) ◽

pp. 25-32

Author(s):

Daniele C. Fernandes ◽

Bruna P. Martins P. Martins ◽

David L. F. Medeiros ◽

Shirley V. M. Santos ◽

Carlos R. M. Gayer ◽

...

Keyword(s):

Acetic Acid ◽

Fatty Acid ◽

Neurogenic Inflammation ◽

Ethics Committee ◽

Antinociceptive Activity ◽

Male Mice ◽

Hot Plate ◽

Fatty Acid Derivatives ◽

Tail Immersion ◽

Anti Inflammatory

Introduction: Echinodorus macrophyllus (Kunth) Micheli,Alismataceae, commonly known as “chapéu de couro”, isused in the treatment of various inflammatory conditions.The aim of this study was to evaluate the antinociceptiveand anti-inflammatory neurogenic potential and perform thephytochemical analysis of its hexanic extract (HEEm). Materialand methods: The HEEm was obtained by maceration ofdried leaves with hexane (100 g d.w./2 L). Its composition wasdetermined by GC-MS (DB1 column) by comparison of retentionindices in the database and literature. The antinociceptivepotential was evaluated in SW or DBA/1 male mice usingchemical (acetic acid and formalin), thermal (tail immersionand hot plate tests) and topical (xylene) nociception models,all approved by the Ethics Committee (CEA-IBRAG). Results:HEEM presented antinociceptive activity in the model of:acetic acid-induced writhing (52%; 25mg/kg); tail immersion(60 and 90 minutes; 50 mg/kg); hot-plate in 60 minutes (25and 100mg/kg) and 120 minutes (25mg/kg); formalin tests, atthe neurogenic (63.4%, 100mg/kg), and inflammatory (50%;50 and 100mg/kg) phases; and in neurogenic inflammationinduced by xylene (88.3%; 100mg/kg). These activities seem tobe related to the terpene and fatty acid derivatives evidencedby GC-MS. Discussion: HEEm presented antinociceptive, aswell as anti-inflammatory, activity by central and peripheralmechanisms, It consists of terpenic and fatty acid derivatives,described in the literature as antioxidants, anti-inflammatory,and antinociceptives. Conclusions: HEEm showed antinociceptiveactivity in all models, which can be related to thepresence of terpenic and fatty acid derivatives.Keywords: Echinodorus macrophyllus; Nociception;Neurogenic inflammation; Phytochemistry.

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Analgesic and Anti-inflammatory Activity of Ficus glomerata in Experimental Animal Models

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.3.10 ◽

2011 ◽

Vol 4 (3) ◽

pp. 1501-1505

Author(s):

Cylma Menezes ◽

Kunal G ◽

Reema N ◽

Satyanarayana D ◽

Jagadish K

Keyword(s):

Analgesic Activity ◽

Inflammatory Activity ◽

Significant Activity ◽

Paw Edema ◽

Hot Plate ◽

Plate Method ◽

Swiss Albino Mice ◽

Albino Mice ◽

Anti Inflammatory ◽

Ficus Glomerata

The anti-inflammatory and analgesic activity of various extracts of Ficus glomerata Roxb. were evaluated in experimental animals. We have determined the anti-inflammatory and analgesic activity of various extracts of the dried fruits of Ficus glomerata by oral administration at doses of 200 and 400 mg/kg of body weight to healthy animals. The extracts were studied for their anti-inflammatory activity in carageenan induced hind paw edema in rats and the paw volume was measured plethysmometrically after 3 hours of injection. The extracts were also evaluated for analgesic activity using Eddy’s hot plate method in Swiss albino mice. The extracts of Ficus glomerata significantly reduced carageenan induced hind paw edema in rats and analgesic activity evidenced by increase in the reaction time by Eddy’s hot plate method in Swiss albino mice. The extracts showed a similar anti-inflammatory and analgesic effect comparative to the standard drugs indomethacin and diclofenac sodium respectively. The present results indicated the ethanolic extract of Ficus glomerata exhibited more significant activity than other extracts in the treatment of pain and inflammation.

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Analgesic and anti-inflammatory activity of Artemisia extracts on animal models of nociception

Balneo and PRM Research Journal ◽

10.12680/balneo.2021.416 ◽

2021 ◽

Vol 12 (Vol.12, no.1) ◽

pp. 34-39

Author(s):

Bianca IVANESCU ◽

Andreia CORCIOVA ◽

Laurian VLASE ◽

Ana-Maria GHELDIU ◽

Anca MIRON ◽

...

Keyword(s):

Plant Extracts ◽

Inflammatory Activity ◽

Moderate Intensity ◽

Indigenous Species ◽

Thermal Stimulus ◽

Hot Plate ◽

Antinociceptive Action ◽

Tail Immersion ◽

Inflammatory Edema ◽

Anti Inflammatory

Introduction. The study aims to assess the antinociceptive and anti-inflammatory activity of extracts obtained from indigenous species of wormwood: Artemisia absinthium, A. annua, A. vulgaris and A. pontica. Materials and methods. For these experiments, we employed nociception models using thermal stimulus (hot plate and tail immersion tests, t = 52.5 0C; 30, 60 and 90 minutes testing), chemical stimulus (Zymosan-induced abdominal constriction response test, using distinct lots and testing at 60, 90 and 120 minutes after administration of samples) and pressure stimulus (Randall Selitto test) and an inflammation model for the evaluation of inflammatory edema by Plethysmometer test. Groups of 6 Swiss mice / lot were used, receiving by oral administration the plant extracts suspended in 0.1% CMC-Na. The doses were administered in geometric progression. Chemical analyses were performed by HPLC-MS in order to identify bioactive substances present in extracts: methoxylated flavonoids, sesquiterpene lactones, phytosterols and hydroxycinnamic acids. Results and discussion. All plant extracts showed antinociceptive action on the models with thermal stimulus, as demonstrated by the ED50 values obtained at different test times. In the models of nociception with chemical and mechanical stimulus, models based on inflammatory mediation, the studied fractions have partially proved their antinociceptive action. Regarding the degree of inhibition of inflammatory edema, the highest potency was exhibited by Artemisia pontica extract (86.5% inhibition for the dose of 100 mg/kg). Hispidulin and eupatorin, known anti-inflammatory compounds, were identified in all extracts, along with caffeic and chlorogenic acids, stigmasterol, campesterol and β-sitosterol. Conclusions. The obtained results support the use of these plant extracts in moderate intensity pain, triggered by both central and peripheral mechanisms. Keywords: hot-plate test, tail immersion test, abdominal constrictive response, Randall-Selitto test, inflammatory edema,

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In vivo Assessment of the Antinociceptive Activity of Ethanol Bark Extract of Thevetia peruviana (Family: Apocynaceae) in Swiss Albino Mice

International Journal of Neuroscience and Behavioral Science ◽

10.13189/ijnbs.2019.070201 ◽

2019 ◽

Vol 7 (2) ◽

pp. 13-21 ◽

Cited By ~ 1

Author(s):

Moumita Dey ◽

Md. Lokman Hossain ◽

Most. Nazma Parvin

Keyword(s):

Antinociceptive Activity ◽

Bark Extract ◽

Swiss Albino Mice ◽

Thevetia Peruviana ◽

Albino Mice ◽

In Vivo Assessment

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Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava

Clinical Phytoscience ◽

10.1186/s40816-019-0130-2 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Akinyinka O. Alabi ◽

Abayomi M. Ajayi ◽

Osarume Omorogbe ◽

Solomon Umukoro

Keyword(s):

Acetic Acid ◽

Aqueous Extract ◽

Psidium Guajava ◽

Antinociceptive Activity ◽

Hot Plate ◽

Hot Plate Test ◽

Promising Alternative ◽

Plate Test ◽

Ocimum Gratissimum ◽

Anti Inflammatory

Abstract Background To investigate the antinociceptive and anti-inflammatory activities of aqueous extract of a blended mixture of dried leaves of Ocimum gratissimum and Psidium guajava, a traditional analgesic drug polyherbal (TADP) used as a remedy for pain-related conditions. Methods Antinociceptive activity of TADP (100, 200 and 400 mg/kg) was evaluated in the hot plate test and acetic acid-induced nociception in mice while the anti-inflammatory was evaluated in carrageenan-induced paw oedema in rats. Levels of nitrite, myeloperoxidase, glutathione and malondialdehyde were assayed in carrageenan-induced paw tissue. Results TADP (200 and 400 mg/kg) significantly prolong the latency time in the hot-plate test. TADP (100–400 mg/kg) produced a dose-dependent significant inhibition of the acetic-acid induced abdominal constriction. The antinociceptive activity of TADP in the presence of naloxone and atropine was not reversed whereas yohimbine and glibenclamide significantly reversed it. TADP (100, 200 and 400 mg/kg) significantly reduced the swelling in the carrageenan-induced oedema model and also produced a reduction in the nitrite and myeloperoxidase level. TADP (400 mg/kg) significantly reduced malondialdehyde concentration and increase glutathione level in the carrageenan-induced rat paw. TADP significantly decrease the number of cellular infiltrates in the histopathological assessment. Conclusion These results indicate that polyherbal product containing blended leaves of Ocimum gratissimum and Psidium guajava possess antinociceptive and anti-inflammatory properties, hence represents a promising alternative remedy in inflammation-induced pain.

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Bioactivities of the ethanol extract from Ageratum fastigiatum branches: antioxidant, antinociceptive and anti-inflammatory

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201620150246 ◽

2016 ◽

Vol 88 (3) ◽

pp. 1471-1484

Author(s):

GLAUCIEMAR DEL-VECHIO-VIEIRA ◽

BRUNA C.S. SANTOS ◽

MARIA SILVANA ALVES ◽

AÍLSON L.A. ARAÚJO ◽

CÉLIA H. YAMAMOTO ◽

...

Keyword(s):

Antinociceptive Effect ◽

Folk Medicine ◽

Reducing Power ◽

Ethanol Extract ◽

Immersion Test ◽

Significant Inhibition ◽

Paw Edema ◽

Hot Plate ◽

Tail Immersion ◽

Anti Inflammatory

ABSTRACT The present study was designed to investigate the antioxidant, antinociceptive and anti-inflammatory activities of the ethanol extract from Ageratum fastigiatum branches. Phytochemical screening and total phenol and flavonoid contents were determined. The antioxidant activity was assessed by 2,2-diphenyl-1-pycrilhydrazin (DPPH) and iron reducing power methods. The antinociceptive effect was evaluated using the acetic acid-induced writhing, formalin, hot plate and tail immersion assays; while the carrageenan-induced paw edema and pleurisy tests were performed to examine the anti-inflammatory activity against acute inflammation. The extract revealed the presence of flavonoids, tannins, coumarins, terpenes, sterols and saponins. Expressive levels of total phenols and flavonoids and a promising antioxidant effect were quantified. At the doses of 50, 100 and 200 mg/kg, the extract inhibited the writhing, reduced both phases of paw licking time and increased the reaction time on the hot plate. In the tail immersion test, the extract (50, 100 and 200 mg/kg) caused a significant inhibition of pain. In these doses, the paw edema, exudate volume and leucocyte mobilization were significantly reduced. These results suggest that A. fastigiatum can be an active source of substances with antioxidant, antinociceptive and anti-inflammatory activities, adding scientific support to the appropriate use in the Brazilian folk medicine.

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