Effect of Debarking Water from Norway Spruce (Picea abies) on the Growth of Five Species ofWood-Decaying Fungi

Abstract Norway spruce (Picea abies) debarking water is an aqueous extract obtained as waste from the debarking of logs at paper mills. The debarking water contains a mixture of natural compounds that can exhibit diverse biological activities, potentially including fungicidal activity on some species of wood-decaying fungi. Thus, we investigated the growth rates of such fungi on agar plates to which debarking water extracts had been added. The experiment included five wood-decaying fungi, viz. Gloeophyllum sepiarium, Oligoporus lateritius, Ischnoderma benzoinum, Junghuhnia luteoalba, and Phlebia sp. Growth reduction was observed for all species at the highest tested concentrations of freeze-dried and ethanol-extracted debarking water, the ethyl acetate-soluble fraction and the diethyl ether-soluble fraction. However, the magnitude of the effect varied between different species and strains of individual species. The brown-rot fungi G. sepiarium and O. lateritius were generally the most sensitive species, with the growth of all tested strains being completely inhibited by the ethyl acetate-soluble fraction. These results indicate that development of antifungal wood-protecting agents from debarking water could potentially be a way to make use of a low-value industrial waste.

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Rapid Identification of Aldose Reductase Inhibitory Compounds fromPerilla frutescens

BioMed Research International ◽

10.1155/2013/679463 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 13

Author(s):

Ji Hun Paek ◽

Kuk Hyun Shin ◽

Young-Hee Kang ◽

Jae-Yong Lee ◽

Soon Sung Lim

Keyword(s):

Ethyl Acetate ◽

Chlorogenic Acid ◽

Aldose Reductase ◽

Rosmarinic Acid ◽

High Speed ◽

Soluble Fraction ◽

Biological Activities ◽

Methanol Extracts ◽

Chemical Structures ◽

Inhibitory Compounds

The ethyl acetate (EtOAc) soluble fraction of methanol extracts ofPerilla frutescens(P. frutescens) inhibits aldose reductase (AR), the key enzyme in the polyol pathway. Our investigation of inhibitory compounds from the EtOAc soluble fraction ofP. frutescenswas followed by identification of the inhibitory compounds by a combination of HPLC microfractionation and a 96-well enzyme assay. This allowed the biological activities to be efficiently matched with selected HPLC peaks. Structural analyses of the active compounds were performed by LC-MSn. The main AR inhibiting compounds were tentatively identified as chlorogenic acid and rosmarinic acid by LC-MSn. A two-step high speed counter current chromatography (HSCCC) isolation method was developed with a solvent system of n-hexane-ethyl acetate-methanol-water at 1.5 : 5 : 1 : 5, v/v and 3 : 7 : 5 : 5, v/v. The chemical structures of the isolated compounds were determined by1H- and13C-nuclear magnetic resonance spectrometry (NMR). The main compounds inhibiting AR in the EtOAc fraction of methanol extracts ofP. frutescenswere identified as chlorogenic acid (2) (IC50= 3.16 μM), rosmarinic acid (4) (IC50= 2.77 μM), luteolin (5) (IC50= 6.34 μM), and methyl rosmarinic acid (6) (IC50= 4.03 μM).

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Phytochemical and Biological Studies on Bark Extract of Aporosa wallichii Hook.f.

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v19i2.50629 ◽

2020 ◽

Vol 19 (2) ◽

pp. 139-143

Author(s):

Kazi Ruhullah Shahriar ◽

Sirajis Salekin ◽

Quazi Sufia Islam ◽

Monira Ahsan ◽

Choudhury M Hasan

Keyword(s):

Ethyl Acetate ◽

Petroleum Ether ◽

Soluble Fraction ◽

Ethyl Acetate Extract ◽

Biological Activities ◽

Radical Scavenging ◽

Chemical Characterization ◽

Bark Extract ◽

Separation And Purification ◽

Biological Studies

The ethyl acetate extract of bark of Aporosa wallichii Hook.f. was extensively investigated for the chemical characterization of secondary metabolites and for biological activities. After repeated chromatographic separation and purification, two compounds were elucidated as a phenolic compound ferulic acid (1) and an uncommon triterpene glut-5(6)-en-3β-ol (2) from the ethyl acetate extract of barks of A. wallichii by using high field NMR analyses. This is the first report of isolation of both compounds from this plant species. No significant antimicrobial activity was observed for any fraction after Kupchan partitioning of the extract. During the antioxidant activity assay, the ethyl acetate soluble fraction of A. wallichii demonstrated significant DPPH radical scavenging capacity with an IC50 value of 1.25 μg/mL. The other fractions viz. petroleum ether, aqueous and chloroform soluble fractions exhibited moderate, mild, and weak activity respectively. The petroleum ether soluble fraction demonstrated maximum thrombolytic property (51.33%) which was similar to that of standard streptokinase (66.81%). Dhaka Univ. J. Pharm. Sci. 19(2): 139-143, 2020 (December)

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Premature failure of utility poles in Switzerland and Germany related to wood decay basidiomycetes

Holzforschung ◽

10.1515/hf-2016-0134 ◽

2017 ◽

Vol 71 (3) ◽

pp. 241-247 ◽

Cited By ~ 7

Author(s):

Javier Ribera ◽

Mark Schubert ◽

Siegfried Fink ◽

Marco Cartabia ◽

Francis W.M.R. Schwarze

Keyword(s):

Picea Abies ◽

Norway Spruce ◽

Treated Wood ◽

Wood Decay ◽

Brown Rot ◽

Wood Preservatives ◽

Premature Failure ◽

Utility Pole ◽

Utility Poles ◽

Cu Tolerance

Abstract In contact with soil, copper (Cu) formulations as preservatives are expected to inhibit wood decay by fungi and other soil-borne microorganisms. However, Cu-resistant brown-rot (BR) fungi lead to premature failures of utility poles at some sites. In this study, the service lives of 111 utility poles of Norway spruce (Picea abies (L.) H. Karst) (73 from Switzerland and 38 from Germany) impregnated with Cu-based wood preservatives were investigated. Three segments of each utility pole were analyzed. The severity of decay was dependent on the preservative formulation. BR fungi and in particular Antrodia species were predominantly isolated from utility poles that were not treated with a co-biocide, e.g. boron (B). Cu-sensitivity of several isolated BR fungi was confirmed in studies on Cu-amended medium and in Cu-treated wood. Isolates of Fibroporia vaillantii and Serpula himantioides showed a higher Cu-tolerance than the highly Cu-tolerant Empa isolate Rhodonia placenta (Empa 45) or Antrodia serialis.

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