THE ANTI-INFLAMMATORY EFFECT OF GARDI PATCH IN CARRAGEENAN-INDUCED PAW EDEMA OF RATS

Gardi -5 has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The drug has been studied in chemical-pharmacological research. We have a new natural patch from the prepared Gardi-5. The present work was undertaken to evaluate the traditional drug Gardi patch for its anti-inflammatory activity.The method of Miho Sekiquichi was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Gardi patch and Ketoprofen patch one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the sub plantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 30, 60, 120, 180, 240, 300 mins using Plethysmometer (UGO Basile, Italy). The right hind paw was injected with 0.1 ml of vehicle.The Gardi patch significantly (p=0.000) inhibited carrageenan induced rat paw edema as compared to control group. In assay data, the TNF-α, PGE2 secretion in serum were highly elevated by carrageenan induction but administration of Gardi patch significantly reduced serum secretion of inflammatory mediators as compared to vehicle group. Gardi patch has an anti-inflammatory properties.

Download Full-text

Ànalgesic and anti-inflammatory activities of traditional mongolian drug Garidi-5

Current Issues in Pharmacy and Medical Sciences ◽

10.12923/j.2084-980x/26.1/a.25 ◽

2015 ◽

Vol 26 (1) ◽

pp. 114-117

Keyword(s):

Vehicle Group ◽

Control Group ◽

Acorus Calamus ◽

Paw Edema ◽

Rat Paw Edema ◽

Tnf Α ◽

Traditional Drug ◽

Anti Inflammatory ◽

The Right ◽

Rat Paw

Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz., Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The present work was undertaken to evaluate the traditional drug Garidi-5 for its anti-inflammatory activity. Methods. The method of Winter et al. was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Garidi-5 (20, 80 and 200 mg/kg, p.o.) and Indometacin (10 mg/kg) one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the subplantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 0.5, 2, 4 h using Plethysmometer (UGO Basile, Italy)). The right hind paw was injected with 0.1 ml of vehicle. Results. The Garidi-5 (20, 80 and 200 mg/kg) significantly (P<0.01) inhibited carrageenan induced rat paw edema as compared to control group. Maximum inhibition of paw edema was observed with Garidi-5 (80 and 200 mg/kg) at 4 h when compared to the control group. In assay data, the TNF-α secretion in serum were highly elevated by carrageenan induction but administration of Garidi-5 significantly reduced serum secretion of inflammatory mediators as compared to vehicle group. Conclusion. In conclusion, these results suggested that Mongolian traditional drug Garidi-5 analgesic and anti-inflammatory effects.

Download Full-text

Anti-inflammatory Activity of Pistacia lentiscus Essential Oil: Involvement of IL-6 and TNF-α

Natural Product Communications ◽

10.1177/1934578x1100601033 ◽

2011 ◽

Vol 6 (10) ◽

pp. 1934578X1100601 ◽

Cited By ~ 2

Author(s):

Andrea Maxia ◽

Cinzia Sanna ◽

Maria Assunta Frau ◽

Alessandra Piras ◽

Manvendra Singh Karchuli ◽

...

Keyword(s):

Essential Oil ◽

Inflammatory Activity ◽

Pistacia Lentiscus ◽

Paw Edema ◽

Rat Paw Edema ◽

Tnf Α ◽

Cotton Pellet ◽

Anti Inflammatory ◽

Factor Α ◽

Rat Paw

The topical anti-inflammatory activity of essential oil of Pistacia lentiscus L. was studied using carrageenan induced rat paw edema and cotton pellet induced granuloma. The effect on serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in rats inserted with cotton pellet was also investigated. On topical application, the oil exhibited a significant decrease in paw edema. The oil also inhibited cotton pellet-induced granuloma, and reduced serum TNF-α and IL-6. It can be concluded that the essential oil of Pistacia lentiscus reduces leukocyte migration to the damaged tissue and exhibits anti-inflammatory activity.

Download Full-text

Evaluation of in vivo anti-inflammatory activity of Ariflex tablet in comparison with diclofenac and aceclofenac tablet in carrageenan induced rat paw edema model

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20213916 ◽

2021 ◽

Vol 9 (10) ◽

pp. 2966

Author(s):

Sanjay Nipanikar ◽

Sohan S. Chitlange

Keyword(s):

Lateral Malleolus ◽

Inflammatory Activity ◽

Control Group ◽

Paw Edema ◽

Surgical Interventions ◽

Locomotor Disability ◽

Rat Paw Edema ◽

Anti Inflammatory ◽

Rat Paw

Background: Osteoarthritis is a major cause of pain and locomotor disability worldwide. Though various pharmacological, mechanical and surgical interventions are used, there is no known cure for OA. The present study was conducted to evaluate anti-inflammatory activity of Ariflex tablet (conceptualized and developed by Ari Healthcare Pvt. Ltd.) in comparison with diclofenac and aceclofenac tablet in carrageenan induced rat paw edema model.Methods: Wistar rats of either sex weighing 150-180 g were taken and divided into 4 groups with 6 animals in each group i.e. group 1 (control group), group 2 (diclofenac tablet), group 3 (aceclofenac tablet) and group 4 (ariflex tablet). The study drugs were orally administered with feeding needle, 30 minutes prior to carrageenan injection. After 30 min 1% w/v of 0.05 ml carrageenan was injected subcutaneously in the rat paw. The paw was marked with ink at the level of lateral malleolus and immersed in mercury up to lateral malleolus mark. The paw volume was measured plethysmographically after injection at 30 minutes, 1 hour, 2 hour, 3 hour, 4 hour and eventually at 5 hour.Results: All the test formulations possess statistically significant (p<0.05) anti-inflammatory activity as compared to control group. The maximum percentage inhibition for Ariflex tablet was 96.97% at the end of 5 hours. When compared to control group, statistically significant reduction of paw edema was observed. The anti-inflammatory activity of Ariflex tablet from 2 hours onwards is comparable to that of diclofenac tablet and aceclofenac tablet.Conclusions: Ariflex tablet possesses significant anti-inflammatory activity.

Download Full-text

EVALUATION OF ANTI-INFLAMMATORY (IN VIVO) ACTIVITY OF ARIFLEX LINIMENT IN COMPARISON WITH DICLOFENAC GEL IN CARRAGEENAN INDUCED RAT PAW EDEMA MODEL

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2021v13i4.42747 ◽

2021 ◽

pp. 67-70

Author(s):

SANJAY NIPANIKAR ◽

S. S. CHITLANGE

Keyword(s):

Significant Inhibition ◽

Lateral Malleolus ◽

Inflammatory Activity ◽

Control Group ◽

Paw Edema ◽

Rat Paw Edema ◽

Edema Model ◽

Anti Inflammatory ◽

Rat Paw

Objective: The present study was conducted to evaluate anti-inflammatory activity of Ariflex liniment (conceptualized and developed by Ari Healthcare Pvt. Ltd) in comparison with Diclofenac gel in carrageenan induced rat paw edema model. Methods: Wistar rats of either sex weighing 150-180 g were taken and divided into 3 groups with 6 animals in each group i.e. Group 1 (Controlled Group), Group 2 (Diclofenac gel) and Group 3 (Ariflex liniment). The study drugs were topically applied 30 min prior to carrageenan injection. After 30 min 1% w/v of 0.05 ml carrageenan was injected subcutaneously in the paw. The paw was marked with ink at the level of lateral malleolus and immersed in mercury up to the lateral malleolus mark. The paw volume was measured plethysmographically, immediately after injection i.e. on 0 min, and then on 30 min,1h, 2h,3h, 4h and 5hr after injection. Results: Diclofenac gel sodium treated group showed significant inhibition (p<0.01) of paw edema at 30 min, 1, 2, 3, 4 and 5th hrs as compared to control group. Ariflex Liniment showed significant inhibition (p<0.05) of paw edema at 30 min, 1, 2, 3, and 4th hrs as compared to the control group. Group treated with Ariflex Liniment did not show any significant decrease in paw edema volume at 5th hrs when compared to the control group. Conclusion: Ariflex Liniment possesses anti-inflammatory activity.

Download Full-text

Anti-inflammatory effect of leaves of Vernonia zeylanica in lipopolysaccharide-stimulated RAW 264.7 macrophages and carrageenan-induced rat paw-edema model

Journal of Ethnopharmacology ◽

10.1016/j.jep.2021.114030 ◽

2021 ◽

pp. 114030

Author(s):

Dilani Rukshala ◽

E. Dilip de Silva ◽

B.V.Loshini R. Ranaweera ◽

Narmada Fernando ◽

Shiroma M. Handunnetti

Keyword(s):

Raw 264.7 ◽

Paw Edema ◽

Raw 264.7 Macrophages ◽

Rat Paw Edema ◽

Edema Model ◽

Anti Inflammatory ◽

Rat Paw ◽

Inflammatory Effect

Download Full-text

TRPV1 Blocker HCRG21 Suppresses TNF-α Production and Prevents the Development of Edema and Hypersensitivity in Carrageenan-Induced Acute Local Inflammation

Biomedicines ◽

10.3390/biomedicines9070716 ◽

2021 ◽

Vol 9 (7) ◽

pp. 716

Author(s):

Oksana Sintsova ◽

Irina Gladkikh ◽

Anna Klimovich ◽

Yulia Palikova ◽

Viktor Palikov ◽

...

Keyword(s):

Transient Receptor Potential ◽

Thermal Hyperalgesia ◽

Control Group ◽

Transient Receptor Potential Vanilloid ◽

Paw Edema ◽

Mice Model ◽

Edema Formation ◽

Sensitivity Experiment ◽

Tnf Α ◽

Anti Inflammatory

Currently the TRPV1 (transient receptor potential vanilloid type 1) channel is considered to be one of the main targets for pro-inflammatory mediators including TNF-α. Similarly, the inhibition of TRPV1 activity in the peripheral nervous system affects pro-inflammatory mediator production and enhances analgesia in total. In this study, the analgesic and anti-inflammatory effects of HCRG21, the first peptide blocker of TRPV1, were demonstrated in a mice model of carrageenan-induced paw edema. HCRG21 in doses of 0.1 and 1 mg/kg inhibited edema formation compared to the control, demonstrated complete edema disappearance in 24 h in a dose of 1 mg/kg, and effectively reduced the productionof TNF-α in both doses examined. ELISA analysis of blood taken 24 h after carrageenan administration showed a dramatic cytokine value decrease to 25 pg/mL by HCRG21 versus 100 pg/mL in the negative control group, which was less than the TNF-α level in the intact group (40 pg/mL). The HCRG21 demonstrated potent analgesic effects on the models of mechanical and thermal hyperalgesia in carrageenan-induced paw edema. The HCRG21 relief effect was comparable to that of indomethacin taken orally in a dose of 5 mg/kg, but was superior to this nonsteroidal anti-inflammatory drug (NSAID) in duration (which lasted 24 h) in the mechanical sensitivity experiment. The results confirm the existence of a close relationship between TRPV1 activity and TNF-α production once again, and prove the superior pharmacological potential of TRPV1 blockers and the HCRG21 peptide in particular.

Download Full-text

Retraction Note: Release-Active Dilutions of Diclofenac Enhance Anti-inflammatory Effect of Diclofenac in Carrageenan-Induced Rat Paw Edema Model

Inflammation ◽

10.1007/s10753-020-01359-x ◽

2020 ◽

Author(s):

Sachin S. Sakat ◽

Kamaraj Mani ◽

Yulia O. Demidchenko ◽

Evgeniy A. Gorbunov ◽

Sergey A. Tarasov ◽

...

Keyword(s):

Paw Edema ◽

Rat Paw Edema ◽

Edema Model ◽

Anti Inflammatory ◽

Rat Paw ◽

Inflammatory Effect

Download Full-text

Anti-Inflammatory Activity of Chrozophora rottleri Extracts on Carrageenan-Induced Rat Paw Edema

International Journal of Pharmacology Phytochemistry and Ethnomedicine ◽

10.18052/www.scipress.com/ijppe.3.20 ◽

2016 ◽

Vol 3 ◽

pp. 20-26

Author(s):

Mallikarjuna Rao Talluri ◽

Battu Ganga Rao ◽

Y. Venkateswaea Rao

Keyword(s):

Ethyl Acetate ◽

Methanol Extract ◽

Bioactive Molecules ◽

Inflammatory Activity ◽

Paw Edema ◽

Standard Drug ◽

Isolation And Characterization ◽

Rat Paw Edema ◽

Anti Inflammatory ◽

Rat Paw

The present study was intended to evaluate Anti-inflammatory activity ofC. rottleriextracts (Hydroalcoholic, Methanol, Ethyl acetate and Hexane). The Anti-inflammatory activity ofC.rottleriextracts at doses of 125mg/kg, 250mg/kg and 500mg/kg using carrageenan induced rat paw edema model compared with standard drug (Indomethacin). The selected plant extracts significantly inhibited paw edema along with the standard drug Indomethacin. Of all extracts, methanol extract produced significant effect on reduction of increased paw thickness, hydro alcoholic and ethyl acetate extracts produced moderate percentage inhibition and hexane extract produced low level of percentage inhibition in reducing paw edema on carrageenan induced rats. In all extracts, methanol extract at a dose of 500mg/kg showed more percentage inhibition i.e . 53.47±2.19. From the results obtained during the study it is concluded thatC. rottlerihaving the bioactive molecule responsible for Anti-inflammatory activity by individually or by combination of different bio-active compounds present in it. Further is necessary for isolation and characterization of bioactive molecules which are responsible for the selected plant biological activities.

Download Full-text