scholarly journals Attempts at active protection of Inonotus obliquus by inoculating birches with its mycelium

2013 ◽  
Vol 41 (2) ◽  
pp. 305-312 ◽  
Author(s):  
Jacek Piętka ◽  
Andrzej Grzywacz

Practical application of active protection methods of <em>Inonotus obliquus</em> (Fr.) Pilát. was examined. Thirty live birches and 15 birch stem sections were artificially inoculated with the fungal mycelium in the Mińsk Forest District (E Poland). The mycelium of <em>I. obliquus</em> was not recorded in the felled test trees and birch stem sections upon the completion of the experiment. Artificial introduction of <em>I. obliquus</em> in the natural environment faces significant problems caused by strong competition from other birch wood-decay fungi. As <em>in vitro</em> studies show (individual biotic effect determination), the fungi examined, occurring on birch trees in nature, are dominant species in relation to <em>I. obliquus</em>.

1985 ◽  
Vol 63 (2) ◽  
pp. 337-339 ◽  
Author(s):  
Elmer L. Schmidt

Influences of eight saturated aliphatic acids (C5–C10, C12, and C16) on basidiospores of four isolates of wood-decay fungi (Poria tenuis and Trametes hispida, white rot fungi, and two isolates of the brown rot fungus Gloeophyllum trabeum) were observed in vitro. Spore responses after 24 h on malt extract agar containing 10, 102 or 103 ppm of each acid included normal germination, delay of germ tube emergence, vacuolation and degeneration of spore cytoplasm, and prevention of germ tube development without spore destruction. Acids of chain length C5–C10 prevented spore germination and killed spores of all fungi at concentrations of 20–50 ppm in media, whereas other acids tested were less active. Spore germination assay of decay fungi may prove useful as a screening tool to compare potency of wood preservatives.


2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Chen-Lung Ho

In this study, anti-mildew and anti-wood-decay fungal activities of the leaf and fruits essential oil and its constituents from Juniperus formosana were evaluated in vitro against seven mildew fungi and four wood decay fungi, respectively. The main compounds responsible for the anti-mildew and anti-wood-decay fungal activities were also identified. The essential oil from the fresh leaves and fruits of J. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The leaf oil mainly consisted of α-pinene (41.0%), limonene (11.5%), α-cadinol (11.0%), elemol (6.3%), and β-myrcene (5.8%); the fruit oil was mostly α-pinene (40.9%), β-myrcene (32.4%), α-thujene (5.9%) and limonene (5.9%). Comparing the anti-mildew and anti-wood-decay fungal activities of the oils suggested that the leaf oil was the most effective. For the anti-mildew and anti-wood-decay fungal activities of the leaf oil, the active source compounds were determined to be α-cadinol and elemol.


HortScience ◽  
2010 ◽  
Vol 45 (3) ◽  
pp. 401-408 ◽  
Author(s):  
Manuela Baietto ◽  
A. Dan Wilson

The development of wood decay caused by 12 major root-rot and trunk-rot fungi was investigated in vitro with sapwood extracted from nine ornamental and landscape hardwood and conifer species native to southern temperate regions of North America, Europe, and the lower Mississippi Delta. Wood decay rates based on dry weight loss for 108 host tree–wood decay fungi combinations were compared at 21 °C over 1-year and 2-year incubation periods in the absence of tree-resistance mechanisms. Strains of Armillaria mellea, Ganoderma lucidum, and Heterobasidion annosum exhibited the highest decay potential in most tree species tested. The order of fungi causing the greatest decay varied over time as a result of temporal changes in decay-rate curves. Relative wood durability or resistance to decay generally was greater in gymnosperm than in angiosperm wood types. Quercus nuttallii, Fraxinus pennsylvanica, and Quercus lyrata sustained the highest levels of decay by all fungi. Northern white cedar (Thuja occidentalis) sapwood was most resistant to decay by all rot-fungi tested, sustaining only limited weight loss after 1 and 2 years of decay, although sapwood of Pinus taeda, Liquidambar styraciflua, and Platanus occidentalis had relatively low levels of decay after 2 years. These results in combination with data from portable decay-detection devices provide useful information for the management of tree breakages or failures resulting from wood decay fungi in hazardous landscape trees. Some potential landscaping applications for tree evaluations, risk assessments, and selections for tree-replacement plantings are discussed.


2018 ◽  
Vol 94 (02) ◽  
pp. 109-116 ◽  
Author(s):  
Marta Aleksandrowicz-Trzcińska ◽  
Adam Szaniawski ◽  
Jacek Olchowik ◽  
Stanisław Drozdowski

Holzforschung ◽  
2005 ◽  
Vol 59 (6) ◽  
pp. 620-627 ◽  
Author(s):  
Chi-Lin Wu ◽  
Shih-Chang Chien ◽  
Sheng-Yang Wang ◽  
Yueh-Hsiung Kuo ◽  
Shang-Tzen Chang

Abstract Cadinane-type sesquiterpenes have a wide spectrum of biological activity, but their use as wood preservatives and the structure-activity relationships of their derivatives have not yet been reported. A total of 13 compounds were synthesized from T-cadinol, T-muurolol, and α-cadinol and their chemical structures were confirmed by IR, MS, and 1H and 13C NMR. The antifungal properties of 16 compounds against three wood-decay fungi were evaluated in vitro. α-Cadinol showed strong antifungal activity against Lenzites betulina, Trametes versicolor, and Laetiporus sulphureus (total mean IC50 0.10 mM). Among the derivatives synthesized, 3β-ethoxy-T-muurolol (0.24 mM), 4ξH-cadinan-10β-ol (0.25 mM), 4ξH-muurolan-10β-ol (0.29 mM), and 4ξH-cadinan-10α-ol (0.25 mM) showed good antifungal activity against all fungi tested. Correlation was observed between the antifungal activity of the compounds tested and log P. Furthermore, the presence of an unsaturated double bond and oxygen-containing functional groups in the compounds plays a key role in their antifungal activity. The stereo configuration of cadinane-type sesquiterpenes also influences their antifungal activity. Understanding how the structure of natural compounds relates to their antifungal function is important and may facilitate their application as novel wood preservatives.


2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Yu-Chang Su ◽  
Kuang-Ping Hsu ◽  
Chen-Lung Ho

In this study, cytotoxicity, anti-mildew and anti-wood-decay fungal activities of the bark essential oil and its constituents from Cunninghamia lanceolata var. konishii were evaluated in vitro against three human cancer cell lines, seven mildew fungi, and four wood decay fungi, respectively. The main compounds responsible for the cytotoxicity, anti-mildew, and anti-wood decay fungal activities were also identified. The essential oil from the barks of C. lanceolata var. konishii was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The bark oil mainly consisted of ferruginol (15.1%), β-caryophyllene (11.2%), α-cadinol (10.9%) and cedrol (10.1%). The essential oil exhibited cytotoxic activity against human oral, liver, and lung cancer cells. The active source compounds were α-cadinol, cedrol, β-caryophyllene, and ferruginol. The bark oil was shown to have excellent anti-mildew and anti-wood-decay fungal activities, the active compounds being evaluated as α-cadinol, cedrol, and ferruginol.


Author(s):  
M Bhadra ◽  
A Khair ◽  
MA Hossain ◽  
MM Sikder

An experiment was conducted to isolate a number of biocontrol agent- Trichoderma spp. from infected spawn packets of oyster mushroom at National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh. These bio-control agents were used as antagonist against four wild wood decay fungi of Ganoderma, viz., G. lucidum-1, G. lucidum-2, G. lucidum-3, G. applanatum and two cultivated G. lucidum-4, G.lucidum-6 under in vitro condition. An in vitro trial of Trichoderma spp. against Ganoderma were performed by dual culture, by treating with volatile, non-volatile and naturally untreated metabolites of bio-control agents. In dual culture, all the Trichoderma species showed 70- 100% mycelia inhibition of G. lucidum-1 and G. lucidum-2, 55.6-100% inhibition of G. lucidum-3, 20-66.7% of G. applanatum, 100% of G. lucidum-5, 75-100% of G. lucidum-6. Effects of heat killed extracts of Trichoderma spp. on growth of G. lucidum-2 (wild) and G. lucidum-6 (cultivated) were also evaluated. Fungicides Bavistin and Dithane M-45 were also used to investigate the mycelial growth inhibition of Ganoderma spp.Int. J. Agril. Res. Innov. & Tech. 6 (2): 31-35, December, 2016


2011 ◽  
Vol 280 ◽  
pp. 124-127 ◽  
Author(s):  
Tao Liang ◽  
Shuang Yue Li ◽  
Shu Jun Li ◽  
Li Jun Zhang

Rosin was used as a raw material to prepare N-(3-rosin acyloxy-2-hydroxyl) propyl-N,N diethanolamine. First, rosin was modified with epoxy chloropropane. Then the modified rosin reacted with diethanolamine and N-(3-rosin acyloxy-2-hydroxyl) propyl-N, N diethanolamine was produced under the following conditions: modified rosin and diethanolamine mole ratio of 1:2, reaction temperature of 78°C, and reaction time of 2.5 h. The chemical structure of the product as a rosin amide derivative was identified by Fourier transform infrared spectroscopy (FTIR) and liquid chromatography-mass spectrometry (LC-MS) method. The anti-fungal activity of this rosinyl tertiary amine was determined in vitro against wood decay fungi, Trametes versicolor, Gloeophyllum trabeum and mould fungi, Aspergillus niger and Paecilomyces variot Bainier. The anti-fungal experiment results signified that N-(3-rosin acyloxy-2-hydroxyl) propyl-N,N diethanolamine was active to these fungi at a certain concentration.


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