scholarly journals Protein bodies formation in yellow lupin seeds

2014 ◽  
Vol 53 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Adam Woźny ◽  
Fortunat Młodzianowski ◽  
Barbara Stefaniak
Keyword(s):  
Protein Synthesis ◽  
Seed Development ◽  
Storage Protein ◽  
Cell Walls ◽  
Protein Bodies ◽  
Central Vacuole ◽  
Yellow Lupin ◽  
Storage Lipids ◽  
Lupin Seeds ◽  
Cotyledon Cells

The ultrastructure of cotyledon cells is described at five stages of lupin seed development, distinguished on the basis of their morphological features. It was found that the endoplasmic reticulum nad dictyosomes participate in the synthesis and transport of storage protein, and that protein is deposited in the central vacuole or in newly forming ones. In the investigated zone of lupin cotyledon cells two forms of protein bodies were observed differing in the contrast and compactness. Both were the simple protein, bodies. Parallelly to storage protein synthesis, thickening of the cell walls was observed which may indicate synthesis and deposition of hemicellulose in them. Storage lipids surrounding the protein bodies form in the end stages of seed development.

scholarly journals Rape embryogenesis VI. Formation of protein bodies

2014 ◽  
Vol 56 (4) ◽  
pp. 585-597 ◽  
Author(s):  
Teresa Tykarska
Keyword(s):  
Protein Synthesis ◽  
Brassica Napus ◽  
Seed Coat ◽  
Storage Protein ◽  
Protein Bodies ◽  
Seed Maturation ◽  
Embryo Axis ◽  
Embryo Cells

The storage protein synthesis starts in <em>Brassica napus</em> var. Górczański embryo at the final embryogenesis stage, i.e. in green seeds. Storage protein accumulate in selected zones adjacent to big vacuoles. These vacuoles, as well as surrounding protein zones, are subject to fragmentation. Young aleuron grains originate. They grow occupying sites of declining vacuoles. In mature rape embryo two kinds of protein bodies occur: aleuron grains, well-stainable with protein-specific dyes, and myrosin grains weakly-stainable with them but PAS-positive. Myrosin grains occur earlier than aleuron grains in special cortex and cytoledon cells. Although the first aleuron grains form in outer cells of lateral cap parts and in cortex cells at the hypocotyl-root boundary, they originate most rapidly in endodermis. In the embryo axis aleuron grains form so rapidly that at the beginning of browning of the seed coat most of them are already formed. Aleuron grains developed in all embryo cells accept in those of the youngest columella layers and differentiated procambial strands. The accumulation of storage protein lasts till the end of seed maturation.

Where is the prolamine located in rice endosperm?

1990 ◽  
Vol 48 (3) ◽  
pp. 696-697
Author(s):  
Hsin-Kan Wu ◽  
Mei-Chu Chung
Keyword(s):  
Storage Protein ◽  
Sodium Phosphate ◽  
Recent Experiment ◽  
Protein A ◽  
Uranyl Acetate ◽  
Protein Bodies ◽  
Lead Citrate ◽  
Thin Sections ◽  
Rice Endosperm ◽  
Ethanol Series

In one of our earlier papers (Wu et al. 1978), we suggested that glutelin is the major composition of the round storage protein bodies although they also contain relatively more prolamine than the angular one does. Immunochemical studies of Krishnan et al. (1986) later showed the presence of glutelin in the irregularly-shaped (angular) protein bodies while the prolamines were found in the round ones. Our recent experiment using protein A-gold technique found that prolamine is mainly deposited into the angular protein bodies.Small blocks (1 mm3) of 7 DAF (days after flowering) caryopsis of Orvza perennis were fixed with 3% paraformaldehyde and 3% glutaraldehyde in 0.1M sodium phosphate, pH7.4, dehydrated in a graded ethanol series and infiltrated with Spurr’s resin. Thin sections, after gold labeling, were stained with uranyl acetate and lead citrate. Rabbit antibodies were raised against purified prolamine. Protein A-gold sol complex was prepared based on the technique of Horisberger et al. (1977).

scholarly journals Isolation of a New Cytokinin from Immature Yellow Lupin Seeds

1967 ◽  
Vol 31 (7) ◽  
pp. 795-801 ◽  
Author(s):  
Koichi Koshimizu ◽  
Satoshi Matsubara ◽  
Toshiatsu Kusaki ◽  
Tetsuo Mitsui
Keyword(s):  
Yellow Lupin ◽  
Lupin Seeds

Changes in the activity of phosphoenolpyruvate carboxylating enzymes in germinating yellow lupin seeds

1998 ◽  
Vol 20 (2) ◽  
pp. 119-122 ◽  
Author(s):  
Wiktoria Ratajczak ◽  
Władysław Polcyn ◽  
Teresa Lehmann ◽  
Lech Ratajczak
Keyword(s):  
Yellow Lupin ◽  
Carboxylating Enzymes ◽  
Lupin Seeds

scholarly journals Morphoanatomical and histochemical studies of the seed development of Euterpe oleracea (Arecaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
João Alves Ferreira Pereira ◽  
Ítalo Antônio Cotta Coutinho ◽  
Emanoella Lima Soares ◽  
Arlete Aparecida Soares ◽  
Ana Paula de Souza Caetano ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Seed Development ◽  
Cell Walls ◽  
Palm Tree ◽  
Euterpe Oleracea ◽  
Scientific Interest ◽  
Vast Array ◽  
Future Studies ◽  
Seed Biology ◽  
Fruit Pericarp

Abstract Although the consumption of açaí (Euterpe oleracea) pulp has long been an important component of the diet of the peoples from the Amazon, the açaí palm tree has recently attracted economic and scientific interest because of its vast array of bioactive compounds found in the fruit pericarp. The açaí seeds are the largest byproduct after pulp extraction and have potential for use in ethanol production, but this process is hindered by limited knowledge of seed biology, chemical composition and pattern reserve deposition during seed development. The aim of this work was to describe the morphoanatomical development of the seeds, as well as to identify the main organic compounds stored in the seeds. To achieve this goal, histological and histochemical analyses were performed on developing seeds. Results showed the seed is albuminous, bitegmic and that ingrowths of the seed coat give rise to a ruminate endosperm. Moreover, the nutritive reserves of açaí seeds are found in the endosperm thickened cell walls as reserve polysaccharides. Our findings provide information for future studies dealing with reproductive biology, propagation and the improvement of this profitable crop.

scholarly journals Anatomía de la semilla de Casimiroa edulis (Rutaceae), "zapote blanco", durante su desarrollo

2017 ◽  
pp. 67
Author(s):  
Hilda Araceli Zavaleta-Mancera
Keyword(s):  
Seed Development ◽  
Mature Embryo ◽  
Mature Seed ◽  
Protein Bodies ◽  
Fast Green ◽  
Oil Glands ◽  
Specific Staining ◽  
Starch Grains ◽  
Casimiroa Edulis ◽  
Mexican Species

The seed of the Mexican species, Casimiroa edulis Llave et Lexarza is valued for medicinal purposes. We have studied its anatomy in different stages of development from ovule to mature seed. We applied general staining with safranin and fast green and specific staining for the detection of starch, lipids, lignin, polyphenols, and proteins. Embryo and seed development was also evaluated on the basis of volume. The ovule is sessile, bitegmic, crassinucellate, hemianatropous, with a nucellar curvature of 30-40º and a large hilum. The seed develops a large pachychalaza that covers approximately 70% of the surface. The testa is thin, nonlignified and tanniferous. The mature embryo occupies about 90% of the whole volume of the seed and contains lysigenous oil glands, starch grains and protein bodies. The radicle is not directed exactly at the micropyle. lt is located in the dorsal micropylar third of the seed. Casimiroa edulis had been described as lacking endosperm, but in reality the mature seed has a small amount of endosperm under the pachychalaza.

scholarly journals The influence of jasmonic acid on the amount and the distribution of cysteine proteinase PLCP-2 in healthy and PVYNTN infected potato plants (Solanum tuberosum L.)

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S95-S98
Author(s):  
M. Pompe-Novak ◽  
M. Tušek-Žnidarič ◽  
B. Štrukelj ◽  
M. Ravnikar
Keyword(s):  
Electron Microscopy ◽  
Solanum Tuberosum ◽  
Jasmonic Acid ◽  
Cell Walls ◽  
Cysteine Proteinase ◽  
Solanum Tuberosum L ◽  
Cell Types ◽  
Protein Bodies ◽  
Potato Plants

The localization of cysteine proteinase PLCP-2 was investigated in potato plants (Solanum tuberosum L.) cultivar Désirée by electron microscopy. Healthy and PVY<sup>NTN</sup> infected potato plants were grown in vitro on media with or without a supplement of jasmonic acid. We had already shown that PLCP-2 is present in leaves, stems, tips of shoots and tips of roots of healthy and PVY<sup>NTN</sup> infected plants. It was detected in various cell types in protein bodies in vacuoles, in cytoplasm and in cell walls. There were significantly larger amounts of PLCP-2 in plants grown on medium with a supplement of jasmonic acid in both healthy and virus infected plants. More protein bodies in vacuoles were found in plants grown on medium with addition of jasmonic acid.

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