Isolation of a New Cytokinin from Immature Yellow Lupin Seeds

Adenosine 5ʹ-tetraphosphate phosphohydrolase (EC 3.6.1.14) has been purified to homogeneity from the meal of yellow lupin (Lupinus luteus) seeds. The enzyme is a single polypeptide chain of 25±1 kDa. It catalyses the hydrolysis of a nucleoside 5ʹ-tetraphosphate to a nucleoside triphosphate and orthophosphate, and hydrolysis of tripolyphosphate but neither pyrophosphate nor tetraphosphate. A divalent cation, Mg2+, Co2+, Ni2+ or Mn2+, is required for these reactions. The pH optimum for hydrolysis of adenosine 5ʹ-tetraphosphate (p4A) is 8.2, Vmax is 21±1.7 μmol/min per mg of protein and the Km for p4A is 3±0.6 μM. At saturating p4A concentrations, the rate constant for the reaction is 8.5±0.7 s-1 [at 30 °C, in 50 mM Hepes/KOH (pH 8.2)/5 mM MgCl2/0.1 mM dithiothreitol]. p4A and guanosine 5ʹ-tetraphosphate are hydrolysed at the same rate. Adenosine 5ʹ-pentaphosphate (p5A) is degraded 1/200 as fast and is converted into ATP and two molecules of orthophosphate, which are liberated sequentially. This contrasts with the cleavage of p5A by the lupin diadenosine tetraphosphate hydrolase (EC 3.6.1.17), which gives ATP and pyrophosphate. Zn2+, F- and Ca2+ ions inhibit the hydrolysis of p4A with I50 values of 0.1, 0.12 and 0.2 mM respectively.

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The Plant Aminoacyl-tRNA Synthetases. Purification and Characterization of Valyl-tRNA, Tryptophanyl-tRNA and Seryl-tRNA Synthetases from Yellow-Lupin Seeds

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1975.tb03998.x ◽

1975 ◽

Vol 52 (2) ◽

pp. 301-310 ◽

Cited By ~ 28

Author(s):

Hieronim JAKUBOWSKI ◽

Jerzy PAWELKIEWICZ

Keyword(s):

Purification And Characterization ◽

Yellow Lupin ◽

Trna Synthetases ◽

Aminoacyl Trna Synthetases ◽

Lupin Seeds

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Protein bodies formation in yellow lupin seeds

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1984.001 ◽

2014 ◽

Vol 53 (1) ◽

pp. 3-9 ◽

Cited By ~ 4

Author(s):

Adam Woźny ◽

Fortunat Młodzianowski ◽

Barbara Stefaniak

Keyword(s):

Protein Synthesis ◽

Seed Development ◽

Storage Protein ◽

Cell Walls ◽

Protein Bodies ◽

Central Vacuole ◽

Yellow Lupin ◽

Storage Lipids ◽

Lupin Seeds ◽

Cotyledon Cells

The ultrastructure of cotyledon cells is described at five stages of lupin seed development, distinguished on the basis of their morphological features. It was found that the endoplasmic reticulum nad dictyosomes participate in the synthesis and transport of storage protein, and that protein is deposited in the central vacuole or in newly forming ones. In the investigated zone of lupin cotyledon cells two forms of protein bodies were observed differing in the contrast and compactness. Both were the simple protein, bodies. Parallelly to storage protein synthesis, thickening of the cell walls was observed which may indicate synthesis and deposition of hemicellulose in them. Storage lipids surrounding the protein bodies form in the end stages of seed development.

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Shaping of the fungal communities isolated from yellow lupin seeds (Lupinus luteus L.) throughout storage time

Acta Agrobotanica ◽

10.5586/aa.2005.066 ◽

2012 ◽

Vol 58 (2) ◽

pp. 407-416

Author(s):

Bożena Cwalina-Ambroziak ◽

Tomasz P. Kurowski

Keyword(s):

Crop Rotation ◽

Storage Time ◽

Colletotrichum Gloeosporioides ◽

Lupinus Luteus ◽

Yellow Lupin ◽

Storage Duration ◽

Rhizopus Nigricans ◽

Lupin Seeds ◽

Traditional Cultivars ◽

Yellow Lupine

The object of the experiment were seeds of two traditional cultivars of yellow lupin (Juno and Amulet) cultivated in 1999 in two crop-rotation with 20% and 33% yellow lupine contribution. The quantitative and qualitative composition of the fungal community colonizing the seeds were determined in the laboratory conditions after 0.5-, 1.5- and 2.5-year of storage time. In total 1077 fungal colonies were isolated from the lupin seeds. Fungi representing the species of Penicillium - 29.3%, Alternaria alternata - 26.7% and Rhizopus nigricans - 12.7% were isolated most widely. Among the fungi pathogenic to lupin, the species of Colletotrichum gloeosporioides (16.3% isolates) was dominant. The crop rotation with 20% lupin reduced the number of fungal colonies colonizing the seeds including the pathogens from the species of C. gloeosporioides. Seed disinfection decreased the total number of fungal colonies isolated from both cultivars. Higher number of C. gloeosporioides isolates was found in the combination with disinfected seeds. More fungal colonies were obtained from seeds of cv. Amulet than from those of cv. Juno. The storage duration had an effect on the population and the composition of species of fungi isolated from seeds of yellow lupine. With longer storage population of Penicillium spp. and Rhizopus spp. increased, whereas the population of C. gloeosporioides decreased.

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The Loss of Vigour and Sowing Value of Yellow Lupin Seeds (Lupinus luteus L.) as a Result of Mechanical Harvesting

Plant Breeding and Seed Science ◽

10.1515/plass-2016-0005 ◽

2016 ◽

Vol 73 (1) ◽

pp. 53-62

Author(s):

Agnieszka Faligowska ◽

Grażyna Szymańska ◽

Katarzyna Panasiewicz

Keyword(s):

Electrical Conductivity ◽

Seed Quality ◽

Pearson Correlation ◽

Mean Value ◽

Lupinus Luteus ◽

Pearson Correlation Coefficient ◽

Yellow Lupin ◽

Lupin Seeds ◽

The Mean

AbstractThe aim of the study was to determine the effect of mechanical harvest on the seed quality of yellow lupin. Two effects were studied: the cultivar of yellow lupin (the indeterminate cultivar - Mister and the determinate cultivar - Perkoz) and harvest methods: hand-picked plants with manual shelling of seeds as a control and mechanical shelling with a plot harvester. In comparison with manual shelling of seeds, the mechanical harvest reduced the seed germination and increased the number of abnormal seeds both cultivars. Determinate cultivar was more sensitive, because the loss of its quality was higher (germination of 10%) than indeterminate cultivar (6%). Perkoz had also higher electrical conductivity, with the mean value of 34.3 μS × cm−1× g−1. Manual shelled seeds were characterized by a significantly lower leakage of exudates (24.9 μS × cm−1× g−1), and hence, it exhibited greater vigour than mechanical harvested seeds. Most relations of Pearson correlation coefficient between vigour tests and germination were strong or practically functional.

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