Influence of an increased content of pea and yellow lupin protein in the diet of pigs on meat quality

Background The aim of the study was to test the effect of replacing soya beans with pea and yellow lupin seeds in the diet of pigs on meat quality. The meat for the tests was obtained from 60 fattening crossbred pigs F1 (Polish Large White × Polish Landrace) × F1 (Pietrain × Duroc). The animals belonged to three feeding groups depending on the feed used with the total share of soybeans and its reduction. Water holding capacity, colour, and tenderness were measured and visual and tactile evaluation (colour, marbling and firmness) was performed for meat samples collected from the longissimus lumborum muscle. The chemical composition of the meat and the content of minerals were determined. The content of amino acids, fatty acids and cholesterol was determined. Results There was no significant differences among the assessed physicochemical characteristics of the meat. The obtained meat was of good quality, regardless of the proportion of proteins from legumes in the diet of pigs. The results of the subjective evaluation of meat, its colour, and the content of muscle pigments were uniform in all food groups. Similarly, a uniform, high protein content was found in all tested groups (C—24.98%; E1—24.82%; E2—25.09%) and the content of macro- and micronutrients in the tested meat was equivalent. The profile of fatty acids was not significantly affected by dietary treatment. Palmitoleic acid content was significantly higher (P < 0.05) in the E2 group compared to the E1 group (3.279% compared to 2.844%). The content of amino acids in meat samples was influenced by dietary treatment. Almost all the monitored essential amino acids (threonine, valine, leucine, phenylalanine and lysine) and some of nonessential amino acids’ proportion was increased in the experimental groups (E1, E2). Conclusion Replacing soya bean protein in the pigs’ diet with legume protein (peas and yellow lupin) did not adversely affect meat quality. This applies to both the physicochemical characteristics, the basic composition and the fatty acid profile. The meat of fattening pigs fed with the highest proportion of pea and lupin in the ration was characterized by more favourable proportion of essential amino acids.

Assessment of the differential gene expression in anthracnose treated seedlings of yellow lupin

Seedlings of yellow lupine treated with Colletotrichum lupini isolate were studied by the method of SRAP-analysis with the purpose to assess the differential expression of genes. As a result, the PCR fragment corresponding to tolerant seedlings was found. The genetic determinants found are likely involved in the control of the resistance (tolerance) of lupine plants to anthracnose.

Potash fertilizers in the productivity of fodder crops in conditions of radioactive contamination of the territory

Relevance. In the situation of radioactive contamination, the main factor in reducing the transition of radionuclides from soil to green feed is the use of potash fertilizer, the effect of which on the yield of fodder crops in light soils with low local fertility is not sufficiently studied, therefore, the goal of the studies is to establish the role of potash fertilizer in increasing the yield of fodder crops.Methods. Studies were carried out on lupine yellow, alfalfa variable, sudanese grass, millet, stockade beast, meadow timothy, annual raigras, oats, reed double-tine, meadow oatmeal, national team hedge in the south-west of the Bryansk region on floodplain sod cucumber, sod-podzolista.Results. The highest productivity of 23.1–26.2 t/ha of green mass was found when cultivating yellow lupin. It was found that the best efficiency of fertilizer systems in the cultivation of fodder lands was revealed on floodplain sod stubble sandy soil, for each added kg received from 59.0 to 72.5 kg of crop increase.

Phenotypic characterisation and linkage mapping of domestication syndrome traits in yellow lupin (Lupinus luteus L.)

The transformation of wild plants into domesticated crops usually modifies a common set of characters referred to as ‘domestication syndrome’ traits such as the loss of pod shattering/seed dehiscence, loss of seed dormancy, reduced anti-nutritional compounds and changes in growth habit, phenology, flower and seed colour. Understanding the genetic control of domestication syndrome traits facilitates the efficient transfer of useful traits from wild progenitors into crops through crossing and selection. Domesticated forms of yellow lupin (Lupinus luteus L.) possess many domestication syndrome traits, while their genetic control remains a mystery. This study aimed to reveal the genetic control of yellow lupin domestication traits. This involved phenotypic characterisation of those traits, defining the genomic regions controlling domestication traits on a linkage map and performing a comparative genomic analysis of yellow lupin with its better-understood relatives, narrow-leafed lupin (L. angustifolius L.) and white lupin (L. albus L.). We phenotyped an F9 recombinant inbred line (RIL) population of a wide cross between Wodjil (domesticated) × P28213 (wild). Vernalisation responsiveness, alkaloid content, flower and seed colour in yellow lupin were each found to be controlled by single loci on linkage groups YL-21, YL-06, YL-03 and YL-38, respectively. Aligning the genomes of yellow with narrow-leafed lupin and white lupin revealed well-conserved synteny between these sister species (76% and 71%, respectively). This genomic comparison revealed that one of the key domestication traits, vernalisation-responsive flowering, mapped to a region of conserved synteny with the vernalisation-responsive flowering time Ku locus of narrow-leafed lupin, which has previously been shown to be controlled by an FT homologue. In contrast, the loci controlling alkaloid content were each found at non-syntenic regions among the three species. This provides a first glimpse into the molecular control of flowering time in yellow lupin and demonstrates both the power and the limitation of synteny as a tool for gene discovery in lupins.

Conciliating Traditional Green Manure Technique and Modern Precision Agriculture

Currently the productivity of some European cropping systems is maintained artificially by increasing production factors like mineral fertilizers or pesticides in order to mask the loss of productivity resulting from soil quality degradation. Green manures are known as a good alternative to the use of mineral fertilizers and pesticides. They are an important source of nitrogen and reduce significantly weed invasion. Nevertheless, the literature providing a precise quantification of total nutrients available for plants after incorporation of leguminous species cultivated in Portugal is scarce. This lake of knowledge’s makes farmers worried about hypothetic productivity loss, making them to use excessive complementary amounts of mineral fertilizer. Providing farmers with tools to calculated accurately the reduction of mineral fertilizer will increase their gain and avoid environmental pollution by nutrients lixiviation. Under the scope the international H2020 SoilCare project, a study was conducted during the winter and spring of 2018–2019 at Baixo Mondego valley in Central Portugal, where the main land use is the monoculture of irrigated corn. The nutrient uptake was determined for 5 species of legumes: pre-inoculated Pea (Pisum sativum L.); Yellow Lupin (Lupinus luteus), Red Clover (Trifolium pratense); Balansa Clover (Trifolium michelianum); Arrowleaf Clover (Trifolium vesiculosum) and a control (natural vegetation). For each treatment, we determined total dry matter yield for leguminous and weeds, macronutrients uptake (N and P Total, K, Na, Ca, Mg, S) and micronutrients uptake (Cu, Zn, Fe, Mn). Combining soil analyses, theoretical main crop needs in nutrients (short cycle grain maize) and mineralization rates, we calculated the precise amendment needed to obtain the expected yield of maize in what concerns the macronutrient. The production of total dry matter (leguminous and weeds) was very similar for the 5 treatments e.g., about 7 ton/ha. Nevertheless, considering leguminous production, the higher dry matter yields was obtain for the Arrowleaf Clover and the lower for the Red Clover respectively 5.5 and 3.5 ton/ha. The Macronutrient content (N,P,K) of the leguminous ranged between 22.9 and 28.0 g/kg for N, 2.4 and 3.1 g/kg for P and 12.1 and 31.5 g/kg for K. The Yellow Lupin presented the higher values of N, the clovers the higher values of P and K. The total quantity of macronutrients incorporated in the soil was in average 152 kg/ha for N, 20 kg/ha for P and 170 kg/ha for K with the higher quantities for Arrowleaf Clover. We considered a mineralization coefficient of 0.5 for N and 0.6 for P during the first year and a nutrient extraction of 280 kg/ha of N, 50 kg/ha of P and 245 kg/ha of K, for a production yield of 12 t/ha of corn grain. After correction of plant needs following the soil analyses results, we determinate an optimized fertilization rate of 180-40-0, were the green manure supplies about 35%, 25% and 100% of the NPK extraction of the grain maize.

Analysis of Phospholipids, Lysophospholipids, and Their Linked Fatty Acyl Chains in Yellow Lupin Seeds (Lupinus luteus L.) by Liquid Chromatography and Tandem Mass Spectrometry

Hydrophilic interaction liquid chromatography (HILIC) and electrospray ionization (ESI) coupled to either Fourier-transform (FT) orbital-trap or linear ion-trap tandem mass spectrometry (LIT-MS/MS) was used to characterize the phospholipidome of yellow lupin (Lupinus luteus) seeds. Phosphatidylcholines (PC) were the most abundant species (41 ± 6%), which were followed by lyso-forms LPC (30 ± 11%), phosphatidylethanolamines (PE, 13 ± 4%), phosphatidylglycerols (PG, 5.1 ± 1.7%), phosphatidic acids (PA, 4.9 ± 1.8%), phosphatidylinositols (PI, 4.7 ± 1.1%), and LPE (1.2 ± 0.5%). The occurrence of both isomeric forms of several LPC and LPE was inferred by a well-defined fragmentation pattern observed in negative ion mode. An unprecedented characterization of more than 200 polar lipids including 52 PC, 42 PE, 42 PA, 35 PG, 16 LPC, 13 LPE, and 10 PI, is reported. The most abundant fatty acids (FA) as esterified acyl chains in PL were 18:1 (oleic), 18:2 (linoleic), 16:0 (palmitic), and 18:3 (linolenic) with relatively high contents of long fatty acyl chains such as 22:0 (behenic), 24:0 (lignoceric), 20:1 (gondoic), and 22:1 (erucic). Their occurrence was confirmed by reversed-phase (RP) LC-ESI-FTMS analysis of a chemically hydrolyzed sample extract in acid conditions at 100 °C for 45 min.

The Quality of Carcass and Meat from Geese Fed Diets with or without Soybean Meal

The aim of the study was to compare the carcass and meat quality of geese fed with soybean meal or yellow lupin. In total, 210 White Kołuda® geese were divided into 2 groups (1, soybean meal (SBM); 2, yellow lupin (YL), potato protein, and brewer’s yeast) of 5 replications (21 birds per each). After 16 weeks, 10 geese (5 females, 5 males) from each group were slaughtered. Carcass dissection was done, and major physicochemical traits were analysed (pH, water holding capacity, drip loss, color, and chemical composition of muscles). Weight of leg muscles and their proportion in the carcass were higher (p < 0.05) in SBM. Breast muscles from SBM were characterized by increased (p < 0.05) drip loss, enhanced (p < 0.05) content of protein, water, collagen and connective tissue, and lower (p < 0.05) fat content. Leg muscles from SBM were characterized by higher (p < 0.05) protein and water content but decreased (p < 0.05) salt and fat content compared to YL. The addition of YL (approx. 28%), potato protein, and brewer’s yeast had no negative effect on most meat traits and could partly replace SBM as a protein source in geese feeding. Hence, yellow lupin, potato protein and brewer’s yeast can be used in geese rearing followed by fattening with oats. Some producers do not have the option of using soybean meal. Small-scale farms use their own crop resources, so lupins can be an alternative source of protein for soybean meal.