scholarly journals Molecular evidence for the hybrid origin of Potamogeton ×subrufus Hagstr. (Potamogetonaceae)

2014 ◽  
Vol 83 (3) ◽  
pp. 243-246
Author(s):  
Joanna Zalewska-Gałosz ◽  
Dagmara Kwolek
Keyword(s):  
Herbarium Specimen ◽  
Hybrid Origin ◽  
Molecular Evidence ◽  
Its1 Region ◽  
Successful Amplification ◽  
Polymorphism Pattern ◽  
Locus Classicus

<em>Potamogeton</em> ×<em>subrufus</em> Hagstr. was described as a hybrid between <em>P. lucens</em> L. and <em>P. nodosus</em> Poir.; however, the taxon had not been widely accepted and regarded as conspecific with <em>P.</em> ×<em>fluitans</em> Roth, the hybrid between <em>P. lucens</em> and P. <em>natans</em> L. The origin of <em>P.</em> ×<em>subrufus</em> had been obscured till 2010, when, based on morpho-anatomical treatment, it was shown that <em>P.</em> ×<em>subrufus</em> displays several characters consistently different from those of <em>P.</em> ×<em>fluitans</em>. Here we report a successful amplification and sequencing of nuclear ribosomal ITS1 region from a 115 year-old herbarium specimen of <em>P.</em> ×<em>subrufus</em>, collected in locus classicus by J. Baagöe and preserved in the Herbarium of the Institute of Botany, Jagiellonian University (KRA). Based on the additive polymorphism pattern expressed in the ITS1 sequences of <em>P.</em> ×<em>subrufus</em>, we demonstrate that one of the parents of this hybrid was <em>P. nodosus</em>, as was claimed by Hagström.

ITS sequence variation supports the hybrid origin of Malus toringoides Hughes

2007 ◽  
Vol 85 (7) ◽  
pp. 659-666 ◽  
Author(s):  
Ting-Ting Feng ◽  
Zhi-Qin Zhou ◽  
Jian-Min Tang ◽  
Ming-Hao Cheng ◽  
Shi-Liang Zhou
Keyword(s):  
Sequence Variation ◽  
Gene Tree ◽  
Natural Populations ◽  
Its Sequences ◽  
Nuclear Ribosomal Dna ◽  
Hybrid Origin ◽  
Putative Hybrid ◽  
Molecular Evidence ◽  
Sequence Information ◽  
Aflp Data

Malus toringoides (Rehd.) Hughes was suggested to have originated from hybridization between Malus transitoria Schneid. and Malus kansuensis Rehd., followed by repeated backcrossing to one of the putative parents. In the present study, the sequence information of the internal transcribed spacer (ITS) of nuclear ribosomal DNA (nrDNA) was used to re-examine the origin of this species. A total of 69 accessions from three natural populations (Maerkang, Xiaba and Kehe, Aba Autonomous Region, Sichuan, China) of M. toringoides and 10 accessions of its putative parents were analyzed. Using Malus angustifolia (Ait.) Michx., Malus ioensis (Wood) Britt. and Malus doumeri Chev. as outgroups, our phylogenetic analysis of the ITS sequences of M. toringoides and its putative parents showed that M. toringoides was not monophyletic, and two different types of ITS sequences which were obtained from each of the six accessions of M. toringoides were found to have clustered separately with those of the two putative parent species on the gene tree. A comparison of the sequence variation between M. toringoides and its putative parents revealed an additive variation pattern of ITS sequences in the putative hybrid species. These results are consistent with the previous morphological and amplified fragment length polymorphism (AFLP) data which suggested that M. toringoides was of hybrid origin. Our ITS data provide new molecular evidence for the hybrid origin hypothesis of M. toringoides and these results are of great importance for future study on hybridization, polyploid speciation and evolution of the genus Malus Miller.

Molecular evidence for the hybrid origin of Rosa lichiangensis (Rosaceae)

Phytotaxa ◽  
2015 ◽  
Vol 222 (3) ◽  
pp. 221 ◽  
Author(s):  
Zhangming Zhu ◽  
Xinfen Gao
Keyword(s):  
Significant Role ◽  
Single Copy ◽  
Morphological Characters ◽  
Molecular Data ◽  
Natural Hybridization ◽  
Hybrid Origin ◽  
Molecular Evidence ◽  
Nuclear Marker

Natural hybridization was assumed to play a significant role in the diversification of Rosa. Rosa lichiangensis was suspected to be of hybrid origin based on its intermediate morphological characters between R. soulieana and R. multiflora var. cathayensis. In this study, four chloroplast regions (ndhC-trnV, ndhF-rpl32, ndhJ-trnF, and psbJ-petA) and a single copy nuclear marker (GAPDH) were used to test the hybrid origin of R. lichiangensis. The results from molecular data supported the hybrid origin of R. lichiangensis and further identified R. soulieana as its maternal progenitor and R. multiflora var. cathayensis as the paternal progenitor.

Molecular evidence for hybrid origin of Melastoma intermedium

2012 ◽  
Vol 41 ◽  
pp. 136-141 ◽  
Author(s):  
Seping Dai ◽  
Wei Wu ◽  
Rongshu Zhang ◽  
Ting Liu ◽  
Yunyun Chen ◽  
...  
Keyword(s):  
Hybrid Origin ◽  
Molecular Evidence

scholarly journals Molecular Evidence for Hybrid Origin and Phenotypic Variation of Rosa Section Chinenses

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 996
Author(s):  
Chenyang Yang ◽  
Yujie Ma ◽  
Bixuan Cheng ◽  
Lijun Zhou ◽  
Chao Yu ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Genetic Background ◽  
Morphological Traits ◽  
Taxonomic Status ◽  
Single Copy ◽  
Hybrid Origin ◽  
Biological Research ◽  
Molecular Evidence ◽  
Germplasm Resources ◽  
Wild Type

Rosa sect. Chinenses (Rosaceae) is an important parent of modern rose that is widely distributed throughout China and plays an important role in breeding and molecular biological research. R. sect. Chinenses has variable morphological traits and mixed germplasm. However, the taxonomic status and genetic background of sect. Chinenses varieties remain unclear. In this study, we collected germplasm resources from sect. Chinenses varieties with different morphological traits. Simple sequence repeat (SSR) markers, chloroplast markers, and single copy nuclear markers were used to explore the genetic background of these germplasm resources. We described the origin of hybridization of rose germplasm resources by combining different molecular markers. The results showed that the flower and hip traits of different species in R. sect. Chinenses were significantly different. The SSR analysis showed that the two wild type varieties have different genetic backgrounds. The double petal varieties of R. sect. Chinenses could be hybrids of two wild type varieties. A phylogenetic analysis showed that the maternal inheritance of sect. Chinenses varieties had two different origins. To some extent, variation in the morphological traits of double petal species of R. sect. Chinenses reflects the influence of cultivation process. This study emphasizes that different genetic markers vary in their characteristics. Therefore, analyzing different genetic markers in could provide an insight into highly heterozygous species.

Molecular Evidence for the Hybrid Origin ofBauhinia blakeana(Caesalpinioideae)

2008 ◽  
Vol 50 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Chun Yin Mak ◽  
Ka Shing Cheung ◽  
Pui Ying Yip ◽  
Hoi Shan Kwan
Keyword(s):  
Hybrid Origin ◽  
Molecular Evidence

Molecular evidence for the natural hybrid origin ofPhalaenopsis×intermediaLindl.

2006 ◽  
Vol 81 (4) ◽  
pp. 691-699 ◽  
Author(s):  
C. C. Tsai ◽  
S. C. Huang ◽  
P. L. Huang ◽  
F. Y. Chen ◽  
Y. T. Su ◽  
...  
Keyword(s):  
Hybrid Origin ◽  
Molecular Evidence ◽  
Natural Hybrid

scholarly journals Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Nayana Gunathilaka ◽  
Nilmini Chandrasena ◽  
Tharaka Wijerathna ◽  
Yoshito Fuji ◽  
Deepa Gunasekara ◽  
...  
Keyword(s):  
Gastrointestinal Symptoms ◽  
Strongyloides Stercoralis ◽  
Molecular Evidence ◽  
Pcr Assay ◽  
Its1 Region ◽  
Promising Tool ◽  
Hyperinfection Syndrome ◽  
Polymerase Chain ◽  
Stool Cultures

Strongyloidiasis is caused by the nematode Strongyloides stercoralis which has the unique ability to reproduce and complete its entire life cycle within the human host through its autoinfection cycle. Diagnosis of this infection is important because of its potential to cause fatal hyperinfection syndrome or disseminated infections in those with defective cellular immunity. Parasitological methods based on faecal microscopy and culture often fail to detect low-intensity infections. A multiplex polymerase chain reaction (PCR) assay was developed for the detection of S. stercoralis, Ascaris lumbricoides, and Enterobius vermicularis by designing primers specific for the ITS1 region of ribosomal DNA of S. stercoralis and A. lumbricoides and 18S region of rRNA of E. vermicularis. A 61-year-old patient presented with chronic gastrointestinal and respiratory symptoms and weight loss with a stool microscopy positive for helminth larvae. Stool cultures with the Harada–Mori technique yielded L3 larvae which were identified as S. stercoralis based on morphology. The multiplex PCR performed on DNA extracted from stool elicited the expected band at 129 bp on gel electrophoresis of the PCR yield providing molecular evidence of intestinal strongyloidiasis. The patient’s gastrointestinal symptoms improved with a six-day course of albendazole (400 mg twice daily). Negative posttreatment stool microscopy, culture, and PCR confirmed successful clearance of infection. Molecular-based PCR assay is a promising tool to diagnose and assess the therapeutic efficacy of anthelmintics in intestinal helminthiases such as strongyloidiasis.

Molecular evidence for the interspecific hybrid origin of Ilex × wandoensis

2011 ◽  
Vol 52 (5) ◽  
pp. 516-523 ◽  
Author(s):  
Young Hee Joung ◽  
Deric Picton ◽  
Jae Ok Park ◽  
Mark S. Roh
Keyword(s):  
Interspecific Hybrid ◽  
Hybrid Origin ◽  
Molecular Evidence

scholarly journals Molecular evidence and ecological niche modeling reveal an extensive hybrid zone among three Bursera species (section Bullockia)

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260382
Author(s):  
Eduardo Quintero Melecio ◽  
Yessica Rico ◽  
Andrés Lira Noriega ◽  
Antonio González Rodríguez
Keyword(s):  
Interspecific Hybridization ◽  
Hybrid Zone ◽  
Dna Sequences ◽  
Ecological Niche ◽  
Ecological Niche Modeling ◽  
Molecular Data ◽  
Niche Modeling ◽  
Hybrid Origin ◽  
Putative Hybrid ◽  
Molecular Evidence

The genus Bursera, includes ~100 shrub and trees species in tropical dry forests with its center of diversification and endemism in Mexico. Morphologically intermediate individuals have commonly been observed in Mexican Bursera in areas where closely related species coexist. These individuals are assumed to result from interspecific hybridization, but no molecular evidence has supported their hybrid origins. This study aimed to investigate the existence of interspecific hybridization among three Mexican Bursera species (Bullockia section: B. cuneata, B. palmeri and B. bipinnata) from nine populations based on DNA sequences (three nuclear and four chloroplast regions) and ecological niche modeling for three past and two future scenario projections. Results from the only two polymorphic nuclear regions (PEPC, ETS) supported the hybrid origin of morphologically intermediate individuals and revealed that B. cuneata and B. bipinnata are the parental species that are genetically closer to the putative hybrids. Ecological niche modeling accurately predicted the occurrence of putative hybrid populations and showed a potential hybrid zone extending in a larger area (74,000 km2) than previously thought. Paleo-reconstructions showed a potential hybrid zone existing from the Last Glacial Maximum (~ 21 kya) that has increased since the late Holocene to the present. Future ecological niche projections show an increment of suitability of the potential hybrid zone for 2050 and 2070 relative to the present. Hybrid zone changes responded mostly to an increase in elevational ranges. Our study provides the first insight of an extensive hybrid zone among three Mexican Bursera species based on molecular data and ecological niche modeling.

Molecular evidence for hybrid origin of Aster chusanensis, an endemic species of Ulleungdo, Korea

2014 ◽  
Vol 57 (3) ◽  
pp. 174-185 ◽  
Author(s):  
Hyunchur Shin ◽  
Sang-Hun Oh ◽  
Yongseok Lim ◽  
Chang-Woo Hyun ◽  
Seong-Hyun Cho ◽  
...  
Keyword(s):  
Endemic Species ◽  
Hybrid Origin ◽  
Molecular Evidence
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