Molecular evidence for the interspecific hybrid origin of Ilex × wandoensis

Malus toringoides (Rehd.) Hughes was suggested to have originated from hybridization between Malus transitoria Schneid. and Malus kansuensis Rehd., followed by repeated backcrossing to one of the putative parents. In the present study, the sequence information of the internal transcribed spacer (ITS) of nuclear ribosomal DNA (nrDNA) was used to re-examine the origin of this species. A total of 69 accessions from three natural populations (Maerkang, Xiaba and Kehe, Aba Autonomous Region, Sichuan, China) of M. toringoides and 10 accessions of its putative parents were analyzed. Using Malus angustifolia (Ait.) Michx., Malus ioensis (Wood) Britt. and Malus doumeri Chev. as outgroups, our phylogenetic analysis of the ITS sequences of M. toringoides and its putative parents showed that M. toringoides was not monophyletic, and two different types of ITS sequences which were obtained from each of the six accessions of M. toringoides were found to have clustered separately with those of the two putative parent species on the gene tree. A comparison of the sequence variation between M. toringoides and its putative parents revealed an additive variation pattern of ITS sequences in the putative hybrid species. These results are consistent with the previous morphological and amplified fragment length polymorphism (AFLP) data which suggested that M. toringoides was of hybrid origin. Our ITS data provide new molecular evidence for the hybrid origin hypothesis of M. toringoides and these results are of great importance for future study on hybridization, polyploid speciation and evolution of the genus Malus Miller.

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Molecular evidence for the hybrid origin of Rosa lichiangensis (Rosaceae)

Phytotaxa ◽

10.11646/phytotaxa.222.3.5 ◽

2015 ◽

Vol 222 (3) ◽

pp. 221 ◽

Cited By ~ 1

Author(s):

Zhangming Zhu ◽

Xinfen Gao

Keyword(s):

Significant Role ◽

Single Copy ◽

Morphological Characters ◽

Molecular Data ◽

Natural Hybridization ◽

Hybrid Origin ◽

Molecular Evidence ◽

Nuclear Marker

Natural hybridization was assumed to play a significant role in the diversification of Rosa. Rosa lichiangensis was suspected to be of hybrid origin based on its intermediate morphological characters between R. soulieana and R. multiflora var. cathayensis. In this study, four chloroplast regions (ndhC-trnV, ndhF-rpl32, ndhJ-trnF, and psbJ-petA) and a single copy nuclear marker (GAPDH) were used to test the hybrid origin of R. lichiangensis. The results from molecular data supported the hybrid origin of R. lichiangensis and further identified R. soulieana as its maternal progenitor and R. multiflora var. cathayensis as the paternal progenitor.

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Molecular evidence for hybrid origin of Melastoma intermedium

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2011.12.010 ◽

2012 ◽

Vol 41 ◽

pp. 136-141 ◽

Cited By ~ 11

Author(s):

Seping Dai ◽

Wei Wu ◽

Rongshu Zhang ◽

Ting Liu ◽

Yunyun Chen ◽

...

Keyword(s):

Hybrid Origin ◽

Molecular Evidence

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Molecular Evidence for Hybrid Origin and Phenotypic Variation of Rosa Section Chinenses

Genes ◽

10.3390/genes11090996 ◽

2020 ◽

Vol 11 (9) ◽

pp. 996

Author(s):

Chenyang Yang ◽

Yujie Ma ◽

Bixuan Cheng ◽

Lijun Zhou ◽

Chao Yu ◽

...

Keyword(s):

Genetic Markers ◽

Genetic Background ◽

Morphological Traits ◽

Taxonomic Status ◽

Single Copy ◽

Hybrid Origin ◽

Biological Research ◽

Molecular Evidence ◽

Germplasm Resources ◽

Wild Type

Rosa sect. Chinenses (Rosaceae) is an important parent of modern rose that is widely distributed throughout China and plays an important role in breeding and molecular biological research. R. sect. Chinenses has variable morphological traits and mixed germplasm. However, the taxonomic status and genetic background of sect. Chinenses varieties remain unclear. In this study, we collected germplasm resources from sect. Chinenses varieties with different morphological traits. Simple sequence repeat (SSR) markers, chloroplast markers, and single copy nuclear markers were used to explore the genetic background of these germplasm resources. We described the origin of hybridization of rose germplasm resources by combining different molecular markers. The results showed that the flower and hip traits of different species in R. sect. Chinenses were significantly different. The SSR analysis showed that the two wild type varieties have different genetic backgrounds. The double petal varieties of R. sect. Chinenses could be hybrids of two wild type varieties. A phylogenetic analysis showed that the maternal inheritance of sect. Chinenses varieties had two different origins. To some extent, variation in the morphological traits of double petal species of R. sect. Chinenses reflects the influence of cultivation process. This study emphasizes that different genetic markers vary in their characteristics. Therefore, analyzing different genetic markers in could provide an insight into highly heterozygous species.

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Molecular Evidence for the Hybrid Origin ofBauhinia blakeana(Caesalpinioideae)

Journal of Integrative Plant Biology ◽

10.1111/j.1744-7909.2007.00591.x ◽

2008 ◽

Vol 50 (1) ◽

pp. 111-118 ◽

Cited By ~ 7

Author(s):

Chun Yin Mak ◽

Ka Shing Cheung ◽

Pui Ying Yip ◽

Hoi Shan Kwan

Keyword(s):

Hybrid Origin ◽

Molecular Evidence

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Cytoplasmic male sterility in Desmodium

Australian Journal of Agricultural Research ◽

10.1071/ar9690227 ◽

1969 ◽

Vol 20 (2) ◽

pp. 227 ◽

Cited By ~ 5

Author(s):

KS McWhirter

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Interspecific Hybrid ◽

Pollen Fertility ◽

Pollen Sterility ◽

Hybrid Origin ◽

F1 Hybrids ◽

Male Sterile ◽

Genetic Stocks

A type of male sterility found in two Desmodium plants of probably interspecific hybrid origin was cytoplasmically inherited. The cytoplasmic male-sterile character was incorporated in the tropical legume Desmodium sandwicense by backcrossing. In this genetic background pollen sterility was complete. The male-sterile character was not graft-transmissible, and it produced no detectable pleiotropic effects on growth and development. Desmodium intortum gave restoration of pollen fertility in Fl hybrids with male-sterile lines of D. sandwicense. Restored F1 hybrids produced apparently normal pollen, but tests of functional ability of the pollen disclosed that pollen fertility was less than that of Fl hybrids with normal cytoplasm. Incomplete restoration of fertility was not due to heterozygosity of fertility-restoring genes with gametophytic expression, since fertility-restoring genes were shown to act sporophytically. The results established the occurrence in the legume Desmodium of a system of determination of the male-sterile, fertility-restored phenotypes that is similar to the cytoplasmic male sterility systems described in many other angiosperm plants. A scheme utilizing the genetic stocks produced in this study for commercial production of the interspecific hybrid D. sandwicense x D. intortum as a cultivar is presented.

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Molecular evidence for the natural hybrid origin ofPhalaenopsis×intermediaLindl.

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.2006.11512125 ◽

2006 ◽

Vol 81 (4) ◽

pp. 691-699 ◽

Cited By ~ 8

Author(s):

C. C. Tsai ◽

S. C. Huang ◽

P. L. Huang ◽

F. Y. Chen ◽

Y. T. Su ◽

...

Keyword(s):

Hybrid Origin ◽

Molecular Evidence ◽

Natural Hybrid

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Molecular evidence for the hybrid origin of Potamogeton ×subrufus Hagstr. (Potamogetonaceae)

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2014.024 ◽

2014 ◽

Vol 83 (3) ◽

pp. 243-246

Author(s):

Joanna Zalewska-Gałosz ◽

Dagmara Kwolek

Keyword(s):

Herbarium Specimen ◽

Hybrid Origin ◽

Molecular Evidence ◽

Its1 Region ◽

Successful Amplification ◽

Polymorphism Pattern ◽

Locus Classicus

Potamogeton ×subrufus Hagstr. was described as a hybrid between P. lucens L. and P. nodosus Poir.; however, the taxon had not been widely accepted and regarded as conspecific with P. ×fluitans Roth, the hybrid between P. lucens and P. natans L. The origin of P. ×subrufus had been obscured till 2010, when, based on morpho-anatomical treatment, it was shown that P. ×subrufus displays several characters consistently different from those of P. ×fluitans. Here we report a successful amplification and sequencing of nuclear ribosomal ITS1 region from a 115 year-old herbarium specimen of P. ×subrufus, collected in locus classicus by J. Baagöe and preserved in the Herbarium of the Institute of Botany, Jagiellonian University (KRA). Based on the additive polymorphism pattern expressed in the ITS1 sequences of P. ×subrufus, we demonstrate that one of the parents of this hybrid was P. nodosus, as was claimed by Hagström.

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THE TAXONOMY AND NOMENCLATURE OF THE WHEATS, BARLEYS, AND RYES AND THEIR WILD RELATIVES

Canadian Journal of Botany ◽

10.1139/b59-053 ◽

1959 ◽

Vol 37 (4) ◽

pp. 657-684 ◽

Cited By ~ 95

Author(s):

Wray M. Bowden

Keyword(s):

Interspecific Hybrid ◽

Interspecific Hybrids ◽

Diploid Species ◽

Wild Relatives ◽

Hybrid Origin ◽

Wheat Species ◽

Hybrid Complex ◽

Allohexaploid Wheat ◽

Botanical Varieties

The correct name for the tribe that contains the wheats, barleys, and ryes and their wild relatives is TRITICEAE Dumort. The genus Triticum L. emend. is treated to include both the diploid, allotetraploid, and allohexaploid wheats (Triticum L.), and the diploid species and the allotetraploid and allohexaploid species of interspecific hybrid origin that were formerly placed in the genus Aegilops L. In the geuus Triticum L. emend., there are 10 diploid species; one allotetraploid wheat species of hybrid origin (T. turgidum L. emend.) which includes many cultivars, three botanical varieties, and one auto-allohexaploid form; one allohexaploid wheat (T. × aestivum L. emend.) which is a hybrid complex that includes many cultivars; 10 other allotetraploid or allohexaploid species of interspecific hybrid origin; and numerous other artificial and natural interspecific hybrids. Section HORDEUM of the genus Hordeum L. contains the cultivated barleys and their wild relatives which are all classified under one species, H. vulgare L. emend. The taxonomy of the ryes and the genus Secale L. is considered briefly.

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Molecular evidence and ecological niche modeling reveal an extensive hybrid zone among three Bursera species (section Bullockia)

PLoS ONE ◽

10.1371/journal.pone.0260382 ◽

2021 ◽

Vol 16 (11) ◽

pp. e0260382

Author(s):

Eduardo Quintero Melecio ◽

Yessica Rico ◽

Andrés Lira Noriega ◽

Antonio González Rodríguez

Keyword(s):

Interspecific Hybridization ◽

Hybrid Zone ◽

Dna Sequences ◽

Ecological Niche ◽

Ecological Niche Modeling ◽

Molecular Data ◽

Niche Modeling ◽

Hybrid Origin ◽

Putative Hybrid ◽

Molecular Evidence

The genus Bursera, includes ~100 shrub and trees species in tropical dry forests with its center of diversification and endemism in Mexico. Morphologically intermediate individuals have commonly been observed in Mexican Bursera in areas where closely related species coexist. These individuals are assumed to result from interspecific hybridization, but no molecular evidence has supported their hybrid origins. This study aimed to investigate the existence of interspecific hybridization among three Mexican Bursera species (Bullockia section: B. cuneata, B. palmeri and B. bipinnata) from nine populations based on DNA sequences (three nuclear and four chloroplast regions) and ecological niche modeling for three past and two future scenario projections. Results from the only two polymorphic nuclear regions (PEPC, ETS) supported the hybrid origin of morphologically intermediate individuals and revealed that B. cuneata and B. bipinnata are the parental species that are genetically closer to the putative hybrids. Ecological niche modeling accurately predicted the occurrence of putative hybrid populations and showed a potential hybrid zone extending in a larger area (74,000 km2) than previously thought. Paleo-reconstructions showed a potential hybrid zone existing from the Last Glacial Maximum (~ 21 kya) that has increased since the late Holocene to the present. Future ecological niche projections show an increment of suitability of the potential hybrid zone for 2050 and 2070 relative to the present. Hybrid zone changes responded mostly to an increase in elevational ranges. Our study provides the first insight of an extensive hybrid zone among three Mexican Bursera species based on molecular data and ecological niche modeling.

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