Molecular evidence for the natural hybrid origin ofPhalaenopsis×intermediaLindl.

Malus toringoides (Rehd.) Hughes was suggested to have originated from hybridization between Malus transitoria Schneid. and Malus kansuensis Rehd., followed by repeated backcrossing to one of the putative parents. In the present study, the sequence information of the internal transcribed spacer (ITS) of nuclear ribosomal DNA (nrDNA) was used to re-examine the origin of this species. A total of 69 accessions from three natural populations (Maerkang, Xiaba and Kehe, Aba Autonomous Region, Sichuan, China) of M. toringoides and 10 accessions of its putative parents were analyzed. Using Malus angustifolia (Ait.) Michx., Malus ioensis (Wood) Britt. and Malus doumeri Chev. as outgroups, our phylogenetic analysis of the ITS sequences of M. toringoides and its putative parents showed that M. toringoides was not monophyletic, and two different types of ITS sequences which were obtained from each of the six accessions of M. toringoides were found to have clustered separately with those of the two putative parent species on the gene tree. A comparison of the sequence variation between M. toringoides and its putative parents revealed an additive variation pattern of ITS sequences in the putative hybrid species. These results are consistent with the previous morphological and amplified fragment length polymorphism (AFLP) data which suggested that M. toringoides was of hybrid origin. Our ITS data provide new molecular evidence for the hybrid origin hypothesis of M. toringoides and these results are of great importance for future study on hybridization, polyploid speciation and evolution of the genus Malus Miller.

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Molecular evidence for the hybrid origin of Rosa lichiangensis (Rosaceae)

Phytotaxa ◽

10.11646/phytotaxa.222.3.5 ◽

2015 ◽

Vol 222 (3) ◽

pp. 221 ◽

Cited By ~ 1

Author(s):

Zhangming Zhu ◽

Xinfen Gao

Keyword(s):

Significant Role ◽

Single Copy ◽

Morphological Characters ◽

Molecular Data ◽

Natural Hybridization ◽

Hybrid Origin ◽

Molecular Evidence ◽

Nuclear Marker

Natural hybridization was assumed to play a significant role in the diversification of Rosa. Rosa lichiangensis was suspected to be of hybrid origin based on its intermediate morphological characters between R. soulieana and R. multiflora var. cathayensis. In this study, four chloroplast regions (ndhC-trnV, ndhF-rpl32, ndhJ-trnF, and psbJ-petA) and a single copy nuclear marker (GAPDH) were used to test the hybrid origin of R. lichiangensis. The results from molecular data supported the hybrid origin of R. lichiangensis and further identified R. soulieana as its maternal progenitor and R. multiflora var. cathayensis as the paternal progenitor.

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Iris revolutaColas., natural hybrid origin species: characterization and preservation problems

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology ◽

10.1080/11263500701872986 ◽

2008 ◽

Vol 142 (1) ◽

pp. 162-165

Author(s):

R. Accogli ◽

E. Nutricati ◽

L. Famà ◽

P. Medagli ◽

D. Manno ◽

...

Keyword(s):

Hybrid Origin ◽

Natural Hybrid

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Molecular evidence for hybrid origin of Melastoma intermedium

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2011.12.010 ◽

2012 ◽

Vol 41 ◽

pp. 136-141 ◽

Cited By ~ 11

Author(s):

Seping Dai ◽

Wei Wu ◽

Rongshu Zhang ◽

Ting Liu ◽

Yunyun Chen ◽

...

Keyword(s):

Hybrid Origin ◽

Molecular Evidence

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Molecular Evidence for Hybrid Origin and Phenotypic Variation of Rosa Section Chinenses

Genes ◽

10.3390/genes11090996 ◽

2020 ◽

Vol 11 (9) ◽

pp. 996

Author(s):

Chenyang Yang ◽

Yujie Ma ◽

Bixuan Cheng ◽

Lijun Zhou ◽

Chao Yu ◽

...

Keyword(s):

Genetic Markers ◽

Genetic Background ◽

Morphological Traits ◽

Taxonomic Status ◽

Single Copy ◽

Hybrid Origin ◽

Biological Research ◽

Molecular Evidence ◽

Germplasm Resources ◽

Wild Type

Rosa sect. Chinenses (Rosaceae) is an important parent of modern rose that is widely distributed throughout China and plays an important role in breeding and molecular biological research. R. sect. Chinenses has variable morphological traits and mixed germplasm. However, the taxonomic status and genetic background of sect. Chinenses varieties remain unclear. In this study, we collected germplasm resources from sect. Chinenses varieties with different morphological traits. Simple sequence repeat (SSR) markers, chloroplast markers, and single copy nuclear markers were used to explore the genetic background of these germplasm resources. We described the origin of hybridization of rose germplasm resources by combining different molecular markers. The results showed that the flower and hip traits of different species in R. sect. Chinenses were significantly different. The SSR analysis showed that the two wild type varieties have different genetic backgrounds. The double petal varieties of R. sect. Chinenses could be hybrids of two wild type varieties. A phylogenetic analysis showed that the maternal inheritance of sect. Chinenses varieties had two different origins. To some extent, variation in the morphological traits of double petal species of R. sect. Chinenses reflects the influence of cultivation process. This study emphasizes that different genetic markers vary in their characteristics. Therefore, analyzing different genetic markers in could provide an insight into highly heterozygous species.

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Molecular Evidence for the Hybrid Origin ofBauhinia blakeana(Caesalpinioideae)

Journal of Integrative Plant Biology ◽

10.1111/j.1744-7909.2007.00591.x ◽

2008 ◽

Vol 50 (1) ◽

pp. 111-118 ◽

Cited By ~ 7

Author(s):

Chun Yin Mak ◽

Ka Shing Cheung ◽

Pui Ying Yip ◽

Hoi Shan Kwan

Keyword(s):

Hybrid Origin ◽

Molecular Evidence

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Molecular evidence for a natural hybrid between Isolepis crassiuscula and Isolepis lenticularis (Cyperaceae) in New Zealand

New Zealand Journal of Botany ◽

10.1080/0028825x.2016.1205106 ◽

2016 ◽

Vol 54 (4) ◽

pp. 433-445 ◽

Cited By ~ 2

Author(s):

O Yano ◽

N Tanaka ◽

Y Ito

Keyword(s):

New Zealand ◽

Molecular Evidence ◽

Natural Hybrid

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Molecular evidence for the hybrid origin of Potamogeton ×subrufus Hagstr. (Potamogetonaceae)

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2014.024 ◽

2014 ◽

Vol 83 (3) ◽

pp. 243-246

Author(s):

Joanna Zalewska-Gałosz ◽

Dagmara Kwolek

Keyword(s):

Herbarium Specimen ◽

Hybrid Origin ◽

Molecular Evidence ◽

Its1 Region ◽

Successful Amplification ◽

Polymorphism Pattern ◽

Locus Classicus

Potamogeton ×subrufus Hagstr. was described as a hybrid between P. lucens L. and P. nodosus Poir.; however, the taxon had not been widely accepted and regarded as conspecific with P. ×fluitans Roth, the hybrid between P. lucens and P. natans L. The origin of P. ×subrufus had been obscured till 2010, when, based on morpho-anatomical treatment, it was shown that P. ×subrufus displays several characters consistently different from those of P. ×fluitans. Here we report a successful amplification and sequencing of nuclear ribosomal ITS1 region from a 115 year-old herbarium specimen of P. ×subrufus, collected in locus classicus by J. Baagöe and preserved in the Herbarium of the Institute of Botany, Jagiellonian University (KRA). Based on the additive polymorphism pattern expressed in the ITS1 sequences of P. ×subrufus, we demonstrate that one of the parents of this hybrid was P. nodosus, as was claimed by Hagström.

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The Origin of New Natural hybrid, Goodyera × maximo-velutina (Orchidaceae) from Jeju Island, Korea

Phytotaxa ◽

10.11646/phytotaxa.317.1.6 ◽

2017 ◽

Vol 317 (1) ◽

pp. 61

Author(s):

JI-HYEON SO ◽

NAM-SOOK LEE

Keyword(s):

Nuclear Dna ◽

Natural Populations ◽

Morphological Characteristics ◽

Jeju Island ◽

Hybrid Origin ◽

Leaf Margin ◽

New Taxon ◽

Natural Hybrid ◽

Leaf Venation ◽

Noncoding Regions

We describe a new taxon of Goodyera (Orchidaceae), G. × maximo-velutina from Jeju Island. This taxon is presumed to be a natural hybrid between G. maximowicziana and G. velutina based on morphological characteristics, i.e., leaf margin, leaf venation, hairiness and color of floral bract, pedicel and ovary. We analyzed ITS of nuclear DNA and three noncoding regions of chloroplast DNA (trnH-psbA, trnS-trnG, trnL) for elucidating the hybrid origin of the new species, G. × maximo-velutina, and two putative parental species. The morphological characteristics and illustrations of the species based on the holotype and other specimens from natural populations are provided together with photographs of the habitat.

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