Enzyme profile in samples of bacterial cellulose film treated with Trametes versicolor and Coniophora puteana

2021 ◽  
Vol 116 ◽  
pp. 141-147
Author(s):  
Izabela Betlej
Keyword(s):  
Bacterial Cellulose ◽  
Trametes Versicolor ◽  
Culture Medium ◽  
Hydrolytic Enzymes ◽  
Cellulose Film ◽  
Enzyme Profile ◽  
Coniophora Puteana

Profile of enzymes in samples of bacterial cellulose film treated with Trametes versicolor and Coniophora puteana. The paper presents the results of research showing the profile of hydrolytic enzymes synthesized by fungi that cause decomposition of wood during growth on film samples made of bacterial cellulose. The performed analyzes allowed for the conclusion that the presence of cellulose in the culture medium stimulates the fungal cells to synthesize full groups of enzymes that are not produced on the agarmaltose medium. Among the synthesized enzymes, both species of fungi produced two enzymes: β - glucosidase and N-acetyl-β - glucuronidase with the highest observed activity.

Incorporation of micro/nanoparticles of PCL with essential oil of Cymbopogon nardus in bacterial cellulose

2018 ◽  
Vol 1 (2) ◽  
pp. 37
Author(s):  
Aline Krindges ◽  
Vanusca Dalosto Jahno ◽  
Fernando Morisso
Keyword(s):  
Particle Size ◽  
Particulate Matter ◽  
Essential Oil ◽  
Zeta Potential ◽  
Bacterial Cellulose ◽  
Culture Medium ◽  
Static Culture ◽  
Cymbopogon Nardus ◽  
Cellulose Membranes

Incorporation studies of particles in different substrates with herbal assets growing. The objective of this work was the preparation and characterization of micro/nanoparticles containing cymbopogon nardus essential oil; and the incorporation of them on bacterial cellulose. For the development of the membranes was used the static culture medium and for the preparation of micro/nanoparticles was used the nanoprecipitation methodology. The incorporation of micro/nanoparticles was performed on samples of bacterial cellulose in wet and dry form. For the characterization of micro/nanoparticles were carried out analysis of SEM, zeta potential and particle size. For the verification of the incorporation of particulate matter in cellulose, analyses were conducted of SEM and FTIR. The results showed that it is possible the production and incorporation of micro/nanoparticles containing essential oil in bacterial cellulose membranes in wet form with ethanol.

Biosynthesis of Carboxymethylated Bacterial Cellulose Composite for Wound Dressing

2011 ◽  
Vol 685 ◽  
pp. 322-326 ◽  
Author(s):  
Jun Wei Yu ◽  
Xiao Li Liu ◽  
Chang Sheng Liu ◽  
Dong Ping Sun
Keyword(s):  
Bacterial Cellulose ◽  
Wound Dressing ◽  
Culture Medium ◽  
Acetobacter Xylinum ◽  
Water Holding Capacity ◽  
Water Soluble ◽  
Transmission Rates ◽  
Measurement Results ◽  
Water Holding ◽  
Cellulose Composite

A novel bacterial cellulose (BC) composite (carboxymethylated-bacterial cellulose, CM-BC) was synthesized by Acetobacter xylinum by adding water-soluble carboxymethylated cellulose (CMC) in the culture medium. FTIR results showed that CM-BC is obtained by the incorporation of CMC in the network of BC. Water-holding capacity and water vapor transmission rates (WVTR) of CM-BC and BC are determined. The WVTR of CM-BC is comparable to that of BC, but the water-holding capacity of CM-BC is improved compared with BC. Tensile strengths measurement results showed that the fracture stress of CM-BC is higher than that of BC, indicating that CM-BC have more potential wound dressing applications than BC.

Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film

2006 ◽  
Vol 22 (4) ◽  
pp. 1194-1199 ◽  
Author(s):  
N. Sanchavanakit ◽  
W. Sangrungraungroj ◽  
R. Kaomongkolgit ◽  
T. Banaprasert ◽  
P. Pavasant ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Human Keratinocytes ◽  
Cellulose Film

Effect of the essential oils addition on the rate of bacterial cellulose surface overgrowth by mold fungi

2021 ◽  
Vol 114 ◽  
pp. 43-51
Author(s):  
Marcin Jałoweicki ◽  
Izabela Betlej
Keyword(s):  
Aspergillus Niger ◽  
Essential Oils ◽  
Bacterial Cellulose ◽  
Trichoderma Viride ◽  
Chaetomium Globosum ◽  
Cellulose Film ◽  
Film Sample ◽  
Cinnamon Oil ◽  
Cellulose Pulp ◽  
Cellulose Surface

Effect of the essential oils addition on the rate of bacterial cellulose surface overgrowth by mold fungi. The aim of this study was to determine the effectiveness of protecting films made of bacterial cellulose with essential oils against overgrowth by mold fungi. The cellulose film produced by microorganisms forming a pellicle called SCOBY was modified by introducing into the cellulose pulp essential oils: cinnamon and manuka. Samples of the protected film were treated with mold fungi: Chaetomium globosum, Aspergillus niger and Trichoderma viride. On the basis of the tests conducted, the rate of film overgrowth by mold fungi and the effectiveness criteria of cellulose film protection with essential oils were determined. The addition of cinnamon oil protected the film against the growth of Aspergillus niger and Chaetomium globosum fungi. Manuka oil slowed down the growth of Chaetomium globosum microorganisms on the surface of the bacterial cellulose film sample, but did not protect the samples from overgrowth. The essential oils tested were ineffective against the fungus Trichoderma viride.

scholarly journals Directed Culturing of Microorganisms Using Metatranscriptomics

mBio ◽  
2011 ◽  
Vol 2 (2) ◽  
Author(s):  
Lindsey Bomar ◽  
Michele Maltz ◽  
Sophie Colston ◽  
Joerg Graf
Keyword(s):  
High Throughput ◽  
Culture Medium ◽  
High Throughput Sequencing ◽  
Bacterial Species ◽  
Hydrolytic Enzymes ◽  
Medicinal Leech ◽  
Expression Data ◽  
Rna Seq ◽  
Rna Transcripts ◽  
Uncultured Microorganisms

ABSTRACTThe vast majority of bacterial species remain uncultured, and this severely limits the investigation of their physiology, metabolic capabilities, and role in the environment. High-throughput sequencing of RNA transcripts (RNA-seq) allows the investigation of the diverse physiologies from uncultured microorganisms in their natural habitat. Here, we report the use of RNA-seq for characterizing the metatranscriptome of the simple gut microbiome from the medicinal leechHirudo verbanaand for utilizing this information to design a medium for cultivating members of the microbiome. Expression data suggested that aRikenella-like bacterium, the most abundant but uncultured symbiont, forages on sulfated- and sialated-mucin glycans that are fermented, leading to the secretion of acetate. Histological stains were consistent with the presence of sulfated and sialated mucins along the crop epithelium. The second dominant symbiont,Aeromonas veronii, grows in two different microenvironments and is predicted to utilize either acetate or carbohydrates. Based on the metatranscriptome, a medium containing mucin was designed, which enabled the cultivation of theRikenella-like bacterium. Metatranscriptomes shed light on microbial metabolismin situand provide critical clues for directing the culturing of uncultured microorganisms. By choosing a condition under which the desired organism is rapidly proliferating and focusing on highly expressed genes encoding hydrolytic enzymes, binding proteins, and transporters, one can identify an organism’s nutritional preferences and design a culture medium.IMPORTANCEThe number of prokaryotes on the planet has been estimated to exceed 1030cells, and the overwhelming majority of them have evaded cultivation, making it difficult to investigate their ecological, medical, and industrial relevance. The application of transcriptomics based on high-throughput sequencing of RNA transcripts (RNA-seq) to microorganisms in their natural environment can provide investigators with insight into their physiologies under optimal growth conditions. We utilized RNA-seq to learn more about the uncultured and cultured symbionts that comprise the relatively simple digestive-tract microbiome of the medicinal leech. The expression data revealed highly expressed hydrolytic enzymes and transporters that provided critical clues for the design of a culture medium enabling the isolation of the previously unculturedRikenella-like symbiont. This directed culturing method will greatly aid efforts aimed at understanding uncultured microorganisms, including beneficial symbionts, pathogens, and ecologically relevant microorganisms, by facilitating genome sequencing, physiological characterization, and genetic manipulation of the previously uncultured microbes.

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