scholarly journals Autochthonous Gut Bacteria in Two Indian Air-breathing Fish, Climbing Perch (Anabas testudineus) and Walking Catfish (Clarias batrachus): Mode of Association, Identification and Enzyme Producing Ability

2015 ◽  
Vol 64 (4) ◽  
pp. 361-368 ◽  
Author(s):  
Goutam Banerjee ◽  
Suhas K. Dan ◽  
Ankita Nandi ◽  
Pinki Ghosh ◽  
Arun K. Ray
Keyword(s):  
Cellulase Activity ◽  
Clarias Batrachus ◽  
Cellulolytic Activity ◽  
Bacterial Cells ◽  
Gi Tract ◽  
Bacterial Strains ◽  
Anabas Testudineus ◽  
Activity Maximum ◽  
Air Breathing ◽  
Climbing Perch

Scanning electron microscopy (SEM) was used to define the location of epithelium-associated bacteria in the gastrointestinal (GI) tract of two Indian air-breathing fish, the climbing perch (Anabas testudineus) and walking catfish (Clarias batrachus). The SEM examination revealed substantial numbers of rod shaped bacterial cells associated with the microvillus brush borders of enterocytes in proximal (PI) and distal regions (DI) of the GI tract of both the fish species. Ten (two each from the PI and DI of climbing perch and three each from the PI and DI of walking catfish) isolated bacterial strains were evaluated for extracellular protease, amylase and cellulase production quantitatively. All the bacterial strains exhibited high cellulolytic activity compared to amylolytic and proteolytic activites. Only two strains, CBH6 and CBH7, isolated from the DI of walking catfish exhibited high proteolytic activity. Maximum cellulase activity was exhibited by the strain, CBF2, isolated from the PI of climbing perch. Six most promising enzyme-producing adherent bacterial strains were identified by 16S rDNA gene sequence analysis. The strain ATH1 (isolated from climbing perch) showed high similarity to Bacillus amyloliquefaciens whereas, the remaining five strains (isolated from walking catfish) were most closely related to Bacillus licheniformis.

scholarly journals Is Boldness in Climbing Perch (Anabas testudineus) Sensitive to the Apparatus Used for Measurement?

2020 ◽  
Author(s):  
Prantik Das ◽  
V V Binoy
Keyword(s):  
Freshwater Fish ◽  
Open Field ◽  
Water Surface ◽  
Repeated Exposure ◽  
Anabas Testudineus ◽  
Air Breathing ◽  
Climbing Perch ◽  
Water Plant ◽  
The Subject ◽  
The Impact

AbstractSwimway and open field are the two popular apparatus used for measuring boldness - the propensity to take risky decisions - in various piscine species. The present study compared boldness exhibited by an air breathing freshwater fish climbing perch in a swimway, rectangular open field, intermediate stages between these two apparatus and circular open field. Impact of the modification of the start chamber by providing substratum made up of cobbles and covering the water surface using water plant hydrilla, alone and in combinations on the boldness was also tested. Our results revealed that the apparatus has a significant impact on the boldness in climbing perch. The presence of a shelter in the experimental arena (swimway) and cobble substratum in the start chamber of the apparatus were found to be reducing boldness in this fish, while hydrilla cover on the water surface neutralised the impact of cobble substratum. Repeated exposure and resultant familiarity with the arena increased boldness of climbing perch but the pattern of modification of this behavioural trait exhibited during the course of experiment was divergent across the instruments. These results point towards the need for critically analyzing the influence of instruments used for measuring various behavioural traits and considering biological needs of the subject species while designing the apparatus.

scholarly journals Identification of multi-antibiotic resistant bacteria isolated from Vietnamese climbing perch (Anabas testudineus) on fish farms in Ho Chi Minh City, Vietnam

2021 ◽  
Vol 947 (1) ◽  
pp. 012036
Author(s):  
Thanh Luan Nguyen ◽  
Kien Cuong Tran ◽  
Thu Nha Nguyen Thi ◽  
Lan Phan-Hoang Nguyen ◽  
Nga Tran Thi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Ho Chi Minh City ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Fish Farms ◽  
Antibiotic Resistant Bacteria ◽  
Anabas Testudineus ◽  
Antibiotic Resistant ◽  
Climbing Perch ◽  
Or Genes

Abstract Climbing perch (Anabas testudineus) is considered a cultured species with high economic value and is widely distributed in several Asian countries, including Vietnam. In climbing perch aquaculture, antibiotic and antibacterial agents have been overused, leading to the emergence of antibiotic-resistant bacteria or genes in the aquatic environment as well as in the seafood products. These antibiotic-resistant bacteria and/or genes can then be spread to human through the food chain, generating detrimental consequences for public health and environment. In this study, 14 bacterial strains were isolated from Vietnamese climbing perch collected from various fish farms in Ho Chi Minh City which possess abnormal symptoms on the outside and in their organs. These bacterial strains were then subjected to several biochemical tests to characterize their colony morphologies. Besides, gene sequencing was performed and as compared to the 16S rRNA, 4 bacterial strains were identified with a high percentage in similarity, including Pseudomonas aeruginosa (99.7%), Aeromonas caviae (99.93%), Kosakonia sacchari (100%), and Edwardsiella ictaluri (99.44%). Additionally, these bacteria showed high levels of antibiotic resistance against beta-lactam, tetracycline, and others. Taken together, these results showed that the four identified bacterial strains isolated from Vietnamese climbing perch circulated in fish farms in Ho Chi Minh City. Their current status of multi-antibiotic resistance indicates further alternative sources of antimicrobial agents, for example, natural compounds, to overcome antibiotic resistance. It is then crucial to identify fish diseases, the associated pathogens, and the new alternatives for antibiotics to protect aquatic ecosystems and water resources.

scholarly journals Endoglucanase gene of M42 aminopeptidase/endoglucanase family from thermophilic Bacillus sp. PW1 and PW2 isolated from Tattapani hot spring, Himachal Pradesh, India

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Divyanshi Sharma ◽  
Parul Sharma ◽  
Kamal Dev ◽  
Anuradha Sourirajan
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Cellulase Activity ◽  
Cellulolytic Activity ◽  
Amino Acid Residues ◽  
Bacterial Strains ◽  
Endoglucanase Gene ◽  
Recombinant Production ◽  
Genes Encoding ◽  
Genetic Level

Abstract Background Thermostable cellulases are in constant demand for several biotechnological applications. Two thermophilic bacterial strains PW1 and PW2 isolated from Tattapani hot spring were found to have cellulolytic activity. Subsequently, PW1 and PW2 were identified and mined for genes encoding cellulase activity. Results Sequencing of the 16S rDNA of PW1 and PW2 identified them as Bacillus sp. PW1 (Acc no. KU711837) and Bacillus sp. PW2 (Acc no. KU711838), respectively, which clustered in the clades containing thermophilic members of Bacillus sp. and Geobacillus species. Phylogenetic analysis revealed that despite the morphological and sequence identities, Bacillus sp. PW1 and Bacillus sp. PW2 are different at the genetic level. The cellulase genes (~ 1.1 kb) of the two bacterial strains were amplified using primers designed against related thermophilic cellulases. Sequencing of the cellulase gene amplicons of PW1 and PW2 revealed that they encode proteins of 280 and 206 amino acid residues, respectively. Sequence and domain analysis of the protein products of PW1 and PW2 revealed that they belong to M42 family of aminopeptidase/endoglucanase. The PW2 endoglucanase coding sequence was submitted to Genbank under accession no. MH049504. The structures of putative endoglucanases of PW1 and PW2 were generated using 1VHE.A as template, which showed the presence of vast proportion of random coils. Molecular docking of the modeled endoglucanase proteins with various substrates and products of cellulases showed that carboxymethyl cellulose and maltose exhibit the highest binding affinity, while xylan and glucose the least. Conclusions The two thermophilic bacteria PW1 and PW2 and their endoglucanase gene can be further utilized for recombinant production of thermostable cellulases for their application in industries.

scholarly journals Taxonomical Accounts of Protozoan Parasites of Climbing Perch, Anabas Testudineus in Bangladesh

2021 ◽  
Vol 49 (2) ◽  
pp. 331-345
Author(s):  
Md Aminul Islam Bhuiyan ◽  
Rita Parveen ◽  
Zannatun Nahar Jhinu ◽  
Abdul Jabber Hawlader
Keyword(s):  
Protozoan Parasites ◽  
Dominant Group ◽  
Host Fish ◽  
Anabas Testudineus ◽  
Air Breathing ◽  
Climbing Perch ◽  
Freshwater Bodies

The study was conducted to identify the protozoan parasites in a freshwater indigenous air breathing fish, Anabas testudineus. The host fish was collected during mid of the April 2018 to end of the March 2019 from freshwater bodies of Mymensingh, Kishoregonj, Faridpur, Jashore, Manikganj and Bogura districts of Bangladesh. Five species of phylum myxozoa namely Henneguya gigas, Henneguya mystusia, Henneguyaa cerinae, Henneguya periophthalmusi and Myxidium sp., three species of phylum ciliophora namely Amphileptus disciformis, Epistylisl woffi and Trichodina anabasi and one species of phylum mastigophora namely Trypanosoma anabasi were identified in A. testudineus. Myxozoans (97.55%) were clearly dominant group than chiliophorans (2.45%). Bangladesh J. Zool. 49 (2): 331-345, 2021

scholarly journals Studies on trematode parasites of air breathing fishes of Awangsoi Lake, Manipur

2010 ◽  
Vol 2 (2) ◽  
pp. 242-244 ◽  
Author(s):  
H. Puinyabati ◽  
M. Shomorendra ◽  
Devashish Kar
Keyword(s):  
Channa Punctatus ◽  
Clarias Batrachus ◽  
Heteropneustes Fossilis ◽  
Anabas Testudineus ◽  
Channa Striatus ◽  
Air Breathing ◽  
Systematic Survey ◽  
Clinostomum Complanatum ◽  
Trematode Parasites

The present investigation deals with a systematic survey of trematode parasites of air breathing fishes from Awangsoi Lake collected during 2008-2009. The air breathing fishes found in Awangsoi Lake are Channa punctatus, Clarias batrachus, Channa striatus, Channa orientalis, Anabas testudineus and Heteropneustes fossilis. During the study period the following 5 species of trematodes were collected : Clinostomum complanatum, Allocreadium handia, Allocreadium fasciatusi, Astiotrema reniferum and Genarcopsis goppo. The percentage of abundance was found to be maximum in Astiotrema reniferum and Anabas testudineus harbours the maximum number of parasites.

scholarly journals Can Living with an Alien Invasive Fish, Tilapia, Influence the Shoaling Decision-Making and Exploratory Behaviour of an Air-Breathing Freshwater Fish, the Climbing Perch?

2019 ◽  
Author(s):  
V V Binoy ◽  
Bhagyasree J Ingle ◽  
Aniket Bhattacharya ◽  
Anindya Sinha
Keyword(s):  
Decision Making ◽  
Freshwater Fish ◽  
Freshwater Ecosystems ◽  
Exploratory Behaviour ◽  
Anabas Testudineus ◽  
Air Breathing ◽  
Climbing Perch ◽  
Or Groups ◽  
Social Decision Making ◽  
The Impact

AbstractThe biodiversity of freshwater aquatic ecosystems is threatened by invasive alien species across the world. We studied the impact of the presence of an invasive piscine species, the tilapia Oreochromis mossambicus and acquisition of familiarity with it on the social decision-making and exploratory behaviour of a native, air-breathing, freshwater fish, the climbing perch Anabas testudineus. Our results reveal that the climbing perch did not show any significant preference or aversion to any of the stimulus shoals when unfamiliar monospecific shoals of tilapia, mixed-species shoals of tilapia and climbing perch that were divergent in the composition, or groups comprising only tilapia familiar to the subject fish for a duration of 30, 60, 90 or 120 days, were presented in opposition to a shoal with an equal number of unfamiliar conspecific individuals. No preference for isolated familiar individual tilapia was also observed against its unfamiliar counterpart or a conspecific individual. It is also noteworthy that the propensity of subject climbing perch to initiate exploration of a novel area (a measure of boldness) or exploratory activity and its sociability remained unchanged under different social conditions, including presence of unfamiliar conspecific, familiar conspecific, unfamiliar heterospecific or familiar heterospecific individuals. These results are discussed in the light of ever-increasing levels of invasion by alien fish species and the struggle for survival that currently confront native piscine species in most tropical freshwater ecosystems globally.

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