Characterization of Staphylococcal Cassette Chromosome mec (SCCmec) in Methicillin-Resistant Staphylococcus epidermidis Strains Isolated from Biomaterial-Associated Infections and their Antibiotic Resistance Patterns

Introduction: Staphylococcus epidermidis is a pathogen associated with nosocomial infection in neonatal intensive care units (NICU). This study investigates an outbreak of methicillin resistant S. epidermidis in an NICU in a hospital in Saudi Arabia. Methodology: A total of 41 isolates identified as Gram-positive cocci were obtained from blood culture, umbilical wound swabs and endotracheal aspirate specimens of neonates, of which 29 were identified as S. epidermidis. Bacterial identification at the species level and determination of antibiotic resistance were performed by MicroScan (Dade Behring, USA). Genotyping was completed using randomly amplified polymorphic DNA (RAPD) and the mecA gene was detected by PCR. Results: All 29 S. epidermidis isolates were found to be resistant to oxacillin and were positive for the mecA gene. The isolates showed several multidrug-resistance patterns; the resistance rates to gentamicin, erythromycin, clindamycin, and trimethoprim/sulfamethoxazole were 89.7%, 86.2%, 75.9% and 72.4%, respectively. All isolates were susceptible to vancomycin, teicoplanin, rifampin, synercid, and ciprofloxacin. Several genotypic and phenotypic patterns were detected among the S. epidermidis isolates: antibiogram typing showed seven different patterns, one of which was shared by 65% of the isolates, whereas the most prevalent RAPD genotype was shared by only five S. epidermidis isolates, and did not correlate with antibiotic resistance phenotype. Conclusion: The diverse clonal origin of tested isolates indicates the presence of multiple S. epidermidis strains among neonates in the NICU setting

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State-wide surveillance of antibiotic resistance patterns and spa types of methicillin-resistant Staphylococcus aureus from blood cultures in North Rhine-Westphalia, 2011–2013

Clinical Microbiology and Infection ◽

10.1016/j.cmi.2015.02.013 ◽

2015 ◽

Vol 21 (8) ◽

pp. 750-757 ◽

Cited By ~ 22

Author(s):

C. Cuny ◽

F. Layer ◽

G. Werner ◽

D. Harmsen ◽

I. Daniels-Haardt ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Methicillin Resistant Staphylococcus Aureus ◽

Blood Cultures ◽

Methicillin Resistant ◽

Resistance Patterns ◽

Antibiotic Resistance Patterns ◽

Spa Types

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Trends in antibiotic resistance patterns of methicillin resistant and methicillin sensitive Staphylococcus aureus in Khwaja Yunus Ali Medical College Hospital

KYAMC Journal ◽

10.3329/kyamcj.v9i1.36596 ◽

2018 ◽

Vol 9 (1) ◽

pp. 6-10 ◽

Cited By ~ 1

Author(s):

Abdullah Akhtar Ahmed ◽

Md Abdus Salam ◽

Md Shakhaowat Hossain ◽

Md Babul Aktar ◽

Nusrat Akhtar Juyee ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Pathogenic Bacteria ◽

Current Status ◽

Clinical Samples ◽

Sensitivity Testing ◽

Methicillin Resistant ◽

Resistance Patterns ◽

Antibiotic Resistance Patterns

Background: Antimicrobial resistance in pathogens is a growing concern for health. It is a great concern around the globe about the threat of increasing antimicrobial resistance. In response to this concerns, medical experts have proposed initiatives to curtail the spread of antimicrobial resistance in pathogenic bacteria. We have taken this surveillance study in KYAMCH to contain the spread of antimicrobial resistance.Objectives: To determine the prevalence of MRSA from different clinical samples and to record its current status to commonly used anti Staphylococcus antibiotics.Methods: The clinical samples were taken to the laboratory and within four hours cultured for isolates and identification pathogens. Finally, antibiotic sensitivity testing of the isolated Staphylococcus aureus was performed. Results: More than 80% of MRSA isolates were resistant to ampicillin (98%) followed by amoxicillin, cefixime and azithromycin with 94%, 93.5% and 85% respectively and maximum sensitivity toward tobramycin with 86% followed by 85% gentamicin and 79% meropenem.Conclusion: The detected trend in antibiotic resistance patterns of methicillin-resistant and methicillin-sensitive Staphylococcus aureus in this study is alarming. This has created a huge clinical burden in the hospital settings as well as in the community.KYAMC Journal Vol. 9, No.-1, April 2018, Page 6-10

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Phage typing, PCR amplification for mecA gene, and antibiotic resistance patterns as epidemiologic markers in nosocomial outbreaks of methicillin resistant Staphylococcus aureus

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2009.07.006 ◽

2009 ◽

Vol 16 (1) ◽

pp. 37-49 ◽

Cited By ~ 4

Author(s):

M. Al-Khulaifi Manal ◽

M. Amin Aref Nagwa ◽

Ali A. Al Salamah

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Methicillin Resistant Staphylococcus Aureus ◽

Meca Gene ◽

Pcr Amplification ◽

Phage Typing ◽

Methicillin Resistant ◽

Resistance Patterns ◽

Antibiotic Resistance Patterns

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Staphylococcus epidermidis BV: Antibiotic Resistance Patterns, Physiological Characteristics, and Bacteriophage Susceptibility

Applied Microbiology ◽

10.1128/aem.22.4.693-699.1971 ◽

1971 ◽

Vol 22 (4) ◽

pp. 693-699 ◽

Cited By ~ 5

Author(s):

S. Schaefler

Keyword(s):

Antibiotic Resistance ◽

Staphylococcus Epidermidis ◽

Physiological Characteristics ◽

Resistance Patterns ◽

Antibiotic Resistance Patterns

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The mosaic architecture of Aeromonas salmonicida subsp. salmonicida pAsa4 plasmid and its consequences on antibiotic resistance

10.7287/peerj.preprints.2067 ◽

2016 ◽

Author(s):

Katherine H Tanaka ◽

Antony T Vincent ◽

Mélanie V Trudel ◽

Valérie E Paquet ◽

Michel Frenette ◽

...

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Mobile Genetic Elements ◽

Aeromonas Salmonicida ◽

Genomic Plasticity ◽

Genetic Elements ◽

Resistance Patterns ◽

Antibiotic Resistance Patterns

Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in salmonids, is an issue especially because many isolates of this bacterium display antibiotic resistances, which limit treatments against the disease. Recent results suggested the possible existence of alternative forms of pAsa4, a large plasmid found in A. salmonicida subsp. salmonicida and bearing multiple antibiotic resistance genes. The present study reveals the existence of two newly detected pAsa4 variants, pAsa4b and pAsa4c. We present the extensive characterization of the genomic architecture, the mobile genetic elements and the antimicrobial resistances genes of these plasmids in addition to the reference pAsa4 from the strain A449. The analysis showed differences between the three architectures with consequences on the content of resistance genes. The genomic plasticity of the three pAsa4 variants could be partially explained by the action of mobile genetic elements like insertion sequences. Isolates from Canada and Europe that bore similar antibiotic resistance patterns than pAsa4-bearing strains were genotyped and specific pAsa4 variants could be attributed to phenotypic profiles. pAsa4 and pAsa4c were found in Europe, while pAsa4b was found in Canada. The plasticity of pAsa4 variants related to the acquisition of antibiotic resistance indicates that these plasmids may pose a threat in terms of the dissemination of antimicrobial-resistant A.salmonicida subsp. salmonicida bacteria.

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