scholarly journals Fibroin and Spidroin Thin Film to Support the Attachment and Spread of Human Dermal Fibroblast: The Potency of Skin Tissue Engineering

2021 ◽  
Vol 53 (2) ◽  
pp. 323-340
Author(s):  
Safira Meidina Nursatya ◽  
Anggraini Barlian ◽  
Hermawan Judawisastra ◽  
Indra Wibowo ◽  
Hutomo Tanoto
Keyword(s):  
Thin Film ◽  
Tissue Engineering ◽  
Contact Angle ◽  
Dermal Fibroblast ◽  
Angle Measurement ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Significant Difference ◽  
Hdf Cells

This study aimed to determine the characteristics of scaffolds made of fibroin from Bombyx mori and spidroin from Argiope appensa in supporting the attachment and proliferation of HDF cells on the scaffolds. Thin-film scaffolds were made using the solvent casting technique, where the scaffold is an amalgamation of fibroin, spidroin, PVA, and glycerol. HDF cells were grown on DMEM medium with 10% FBS and 1% antibiotic-antimicotic. Characterization of the scaffolds was performed by using ATR-FTIR, swelling test, contact angle measurement, tensile test, biodegradation, MTT and SEM. The results of the ATR-FTIR analysis showed that the scaffolds contained fibroin, spidroin, PVA, and glycerol. Swelling and contact angle tests showed that all scaffold combinations were hydrophilic. Mechanical properties and in vitro biodegradation tests showed no significant difference among the scaffold combinations. MTT testing showed that all scaffolds could facilitate the attachment of fibroblasts and showed increased viability from day 1, 3, and 5. Scanning electron microscopy showed that the cells in the 70% fibroin and 10% spidroin scaffold had the best cell morphology and the best combination for potential application in skin tissue engineering.

Fabrication and characterization of electrospun polyurethane blended with dietary grapes for skin tissue engineering

2019 ◽  
Vol 50 (5) ◽  
pp. 655-674 ◽  
Author(s):  
Mohan Prasath Mani ◽  
Saravana Kumar Jaganathan
Keyword(s):  
Tissue Engineering ◽  
Wound Dressing ◽  
Blood Compatibility ◽  
Dermal Fibroblast ◽  
Skin Tissue ◽  
Gravimetric Analysis ◽  
Fibrous Scaffold ◽  
Skin Tissue Engineering ◽  
Polyurethane Matrix

The tissue-engineered skin has emerged as a plausible alternative approach to the traditional wound dressing owing to its inherent advantages. Further, in tissue engineering applications, fibrous scaffold obtained through textile technologies is widely attractive. The present study focused on the fabrication of electrospun textile polyurethane wound dressing scaffold incorporated with grape extract. The fabricated composites showed smooth as well as reduced fiber (730 ± 127 nm) and pore (873 ± 51 nm) diameter than the control polyurethane (fiber diameter –890 ± 117 and pore diameter –1064 ± 74 nm) as revealed in the scanning electron microscopy. The formation of hydrogen bond in Fourier transform infrared spectroscopy revealed the interaction between the polyurethane and grape. The addition of grape enhanced the wettability behavior (86° ± 2) and the surface roughness (469 nm) of the polyurethane membrane. Thermal gravimetric analysis and mechanical testing revealed the enhancement of thermal and tensile strength with the incorporation of the grape into the polyurethane matrix. The in vitro blood compatibility and cytocompatibility studies revealed enhanced antithrombogenicity behavior and the non-toxic nature to human dermal fibroblast cells for the fabricated composites than the pristine polyurethane. Hence, the addition of grape into the polyurethane matrix had enhanced the physicochemical characteristics and biocompatibility parameters which could promote this candidate as a valid alternative for skin tissue engineering regeneration.

Hierarchical Decoration of Eggshell Membrane with Polycaprolactone Nanofibers to fabricate a Bilayered Scaffold for Skin Tissue Engineering

MRS Advances ◽  
2019 ◽  
Vol 4 (21) ◽  
pp. 1215-1221
Author(s):  
Preetam Guha Ray ◽  
Pallabi Pal ◽  
Santanu Dhara
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Dermal Fibroblast ◽  
Extraction Process ◽  
Human Dermal Fibroblast ◽  
Eggshell Membrane ◽  
Skin Tissue ◽  
Topographic Analysis ◽  
Skin Tissue Engineering ◽  
Hdf Cells

ABSTRACTEggshell Membrane (ESM) is a naturally occurring proteinaceous microfibrous scaffold capable of mimicking the extracellular matrix (ECM). The extraction methodology deployed for its extraction process impedes its extensive application as a biomaterial in regenerative medicine. Herein, a unique route was deployed to decorate the surface of ESM with electrospun polycaprolactone (PCL) nanofiber in order to ameliorate the above problems and also fabricate a novel ECM mimicking bilayered scaffold for skin tissue engineering applications. Microstructural and surface topographic analysis confirms the formation of bilayered structure with smooth electrospun PCL nanofibers decorated on ESM. Carbodiimide chemistry was utilized to crosslink the two layers. Cytocompatibility evaluation of scaffolds was carried out with Human dermal fibroblast (HDF) cells. The biomimetic architecture and protein rich composition of as fabricated bilayered construct facilitated extensive cell adhesion, proliferation and migration in contrast the bare natural tissue led to impeded cell adhesion.

Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

2018 ◽  
Author(s):  
Antonios Keirouz ◽  
Giuseppino Fortunato ◽  
Anthony Callanan ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Tensile Modulus ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Skin Substitute ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Needleless Electrospinning ◽  
High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

scholarly journals 075 Generation and quantification of oxidized squalene to develop an acne testing model in vitro based on skin tissue engineering

2016 ◽  
Vol 136 (9) ◽  
pp. S173 ◽  
Author(s):  
N. Esselin ◽  
C. Capallere ◽  
C. Meyrignac ◽  
C. Plaza ◽  
C. Coquet ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Testing Model ◽  
Skin Tissue Engineering

WITHDRAWN: Skin Tissue Engineering—In Vivo and In Vitro Applications

2012 ◽  
Vol 39 (1) ◽  
pp. 33-58 ◽  
Author(s):  
Florian Groeber ◽  
Monika Holeiter ◽  
Martina Hampel ◽  
Svenja Hinderer ◽  
Katja Schenke-Layland
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering

scholarly journals Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering

2014 ◽  
Vol 19 (2) ◽  
Author(s):  
Aleksandra Zuber ◽  
Julia Borowczyk ◽  
Eliza Zimolag ◽  
Malgorzata Krok ◽  
Zbigniew Madeja ◽  
...  
Keyword(s):  
Thin Films ◽  
Tissue Engineering ◽  
Human Skin ◽  
Skin Tissue ◽  
Aliphatic Polyesters ◽  
Skin Tissue Engineering ◽  
Cytoskeleton Organization ◽  
Skin Keratinocytes ◽  
Cell Carriers

AbstractDegradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.

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