scholarly journals A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method

2016 ◽  
Vol 65 (9) ◽  
pp. 775-784 ◽  
Author(s):  
Ahmad Mustafa ◽  
Amin Karmali ◽  
Wael Abdelmoez
Keyword(s):  
Olive Oil ◽  
Lipase Activity ◽  
Colorimetric Method ◽  
Activity Measurement ◽  
Microplate Assay ◽  
Oil Emulsion ◽  
Substrate Modification ◽  
Copper Soap

Simplified Turbidimetric Assay for Lipase Activity

1971 ◽  
Vol 17 (12) ◽  
pp. 1150-1153 ◽  
Author(s):  
Zakariya K Shihabi ◽  
Charles Bishop
Keyword(s):  
Olive Oil ◽  
Lipase Activity ◽  
Convenient Method ◽  
Serum Amylase ◽  
Amylase Activity ◽  
Serum Lipase ◽  
Oil Emulsion ◽  
Turbidimetric Assay ◽  
Serum Amylase Activity

Abstract Serum lipase is determined by following turbidity changes during two 1-min intervals after adding serum to an olive oil emulsion containing desoxycholate. The olive oil emulsion is simply prepared and is stable for a month under refrigeration. Our observations confirm the findings of other investigators that increases in serum lipase activity are more accentuated than increases in serum amylase activity during pancreatitis. With the present fast and convenient method, serum lipase appears to be a better test for pancreatitis than is serum amylase.

scholarly journals Automation of the Turbidimetric Determination of Serum Lipase

1978 ◽  
Vol 15 (1-6) ◽  
pp. 326-330 ◽  
Author(s):  
M. Puukka ◽  
Raija Puukka
Keyword(s):  
Olive Oil ◽  
Correlation Coefficient ◽  
Lipase Activity ◽  
Enzyme Activities ◽  
Standard Sample ◽  
Buffer Solution ◽  
Serum Lipase ◽  
Oil Emulsion ◽  
A Value

A turbidimetric procedure for the determination of serum lipase activity has been adapted for use on a System Olli 3000 analyser. In this automated procedure 50 μl of serum and standard sample, and a buffer solution, are pipetted into disposable cuvettes in the measurement block. The block is then preincubated for 15 minutes at 37°C, and after simultaneous addition of the olive oil emulsion, the absorbances of the cuvettes are recorded simultaneously for two minutes at 405 nm. Using the program ‘block standards’ for the calibration of the method the results are ready in graphic form and expressed as U/l within about five minutes of the addition of the olive oil. A total of 60 patient sera can be analysed in half an hour. The method is linear up to at least 1000 U/l, and the reference values are between 11 and 140 U/l. A comparison of the method with the modification of the Cherry-Crandall titrimetric method gives a value of 0·87 for the correlation coefficient and the regression equation y = 0·97x + 12 (n = 191). Within-run precision (CV) for sera with mean enzyme activities of 149 and 354 U/l was found to be 4·2% and 3·4%, respectively (n = 20), and day-to-day precision 5·0% (mean activity = 339 U/l, n = 18).

scholarly journals Low-fat Gouda cheese made from bovine milk-olive oil emulsion: physicochemical and sensory attributes

2015 ◽  
Vol 52 (10) ◽  
pp. 6749-6755 ◽  
Author(s):  
Imène Felfoul ◽  
Salwa Bornaz ◽  
Aroua Baccouche ◽  
Ali Sahli ◽  
Hamadi Attia
Keyword(s):  
Olive Oil ◽  
Bovine Milk ◽  
Sensory Attributes ◽  
Low Fat ◽  
Oil Emulsion ◽  
Gouda Cheese

Intravenous Olive Oil Emulsion

BMJ ◽  
1935 ◽  
Vol 1 (3874) ◽  
pp. 738-738 ◽  
Author(s):  
A. C. Frazer
Keyword(s):  
Olive Oil ◽  
Oil Emulsion

Effects of Intravenous Administration of Fat Emulsion on Formed Blood Elements and Body Temperature in Dogs

1956 ◽  
Vol 187 (1) ◽  
pp. 107-112 ◽  
Author(s):  
H. C. Meng ◽  
Hertha Cress ◽  
John B. Youmans
Keyword(s):  
Body Temperature ◽  
Olive Oil ◽  
Intravenous Administration ◽  
Rectal Temperature ◽  
Fat Emulsion ◽  
Oral Ingestion ◽  
Oil Emulsion ◽  
Heparin Administration ◽  
Healthy Dogs ◽  
Blood Elements

Intravenous administration of a 10% olive oil emulsion or lymph to healthy dogs anesthetized with Nembutal produced marked thrombocytopenia, leucopenia, neutropenia, eosinopenia, lymphopenia, increase in mechanical fragility of erythrocytes, and increase in hematocrit. The increase in mechanical fragility of erythrocytes correlated directly with the degree of lipemia. Heparin administration accelerated the removal of the injected fat from the circulation and hastened the return to normal of the formed blood elements. The changes in the formed blood elements were more marked and persisted longer in the dogs receiving piromen. The changes in the formed blood elements following oral ingestion of olive oil were either mild or insignificant except for the moderate eosinophilia. Elevation of rectal temperature and persistent lymphopenia were observed only in the animals receiving emulsion and piromen. It is concluded that the changes in formed blood elements, including the increase in mechanical fragility of erythrocytes following intravenous administration of fat emulsion, did not seem to correlate with the rise of body temperature.

Fluorometric Method for Measuring Serum Lipase Activity

1975 ◽  
Vol 21 (12) ◽  
pp. 1788-1790 ◽  
Author(s):  
Bernd Rietz ◽  
George G Guilbault
Keyword(s):  
Enzyme Activity ◽  
Olive Oil ◽  
Lipase Activity ◽  
Enzymatic Reaction ◽  
Fluorometric Method ◽  
Reaction Volume ◽  
Serum Lipase ◽  
Fluorescent Indicator ◽  
Substrate Solution ◽  
Emulsified Olive Oil

Abstract We describe a method for determining lipase (triacylglycerol acyl-hydrolase, EC 3.1.1.3) activity in serum. Emulsified olive oil in tris(hydroxymethyl)aminomethane buffer is used as substrate. The course of the enzymatic reaction is followed by measuring the decrease in fluorescence with time of a fluorescent indicator, 4-methylumbelliferone, added to the substrate, caused by the change in the pH of the substrate solution resulting from the reaction. All measurements are performed at 37 °C. The reaction volume is about 2.3 ml, in a Pyrex cuvette. Change of fluorescence and of enzyme activity are linearly related in the range of 77.8 to 389.0 U/liter. An assay can be done in as little as 3 to 5 min, with excellent precision.

Lipoprotein lipase activity in human heart

1963 ◽  
Vol 205 (2) ◽  
pp. 401-404 ◽  
Author(s):  
J. David Schnatz ◽  
John W. Ormsby ◽  
Robert H. Williams
Keyword(s):  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Human Heart ◽  
Ammonium Hydroxide ◽  
Cottonseed Oil ◽  
Heart Tissue ◽  
Lipoprotein Lipase Activity ◽  
Oil Emulsion ◽  
Ventricular Tissue ◽  
Hydrolysis Of

Human ventricular tissue was obtained 11–28 hr post mortem, homogenized in ammonium hydroxide, and tested for its ability to catalyze the hydrolysis of an "activated" cottonseed oil emulsion. Lipolysis, as indicated by a rise in fatty acids, occurred in the presence of a freshly prepared homogenate, but not in the presence of a boiled homogenate. Sodium chloride, 1 m, inhibited the reaction, but sodium fluoride, 0.2 m, did not. An activated cottonseed oil emulsion was more readily hydrolyzed than a "nonactivated" cottonseed oil emulsion. Approximately 50% of the activity of the homogenate sedimented at a gravitational force produced by centrifugation at 800 g for 10 min. The conclusion is that human heart tissue, like animal heart, contains lipoprotein lipase activity and that this activity is associated, in part, with the heavier cellular particles.

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