Abstract
Background: Genetic selection for meat production performance of broilers concomitantly causes excessive abdominal fat deposition, accompanied by several adverse effects, such as the reduction of feed conversion efficiency and reproduction performance. Our previous studies have identified important genes regulating chicken fat deposition, using the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) as an animal model. However, the molecular mechanism underlying fat deposition differences between fat and lean broilers remains largely unknown. Results: Here, we integrated the transcriptome (RNA-Seq) and quantitative proteome (isobaric tags for relative and absolute quantitation, iTRAQ) profiling analyses on abdominal fat tissues from NEAUHLF chicken lines. Differentially expressed genes (2167 DEGs, FDR < 0.01) and proteins (199 DEPs, FDR < 0.05) were identified in lean line compare to fat line, and down-regulated DEGs and DEPs mainly enriched in pathways related to fatty acid metabolism, fatty acid biosynthesis, and PPAR signaling; and numerous up-regulated DEGs and DEPs both enriched in lysosome pathway. Moreover, several key DEGs and DEPs involved in long-chain fatty acid uptake, in situ lipogenesis (fatty acid and cholesterol synthesis), and lipid droplets accumulation were discovered after integrated transcriptome and proteome analysis. Conclusions: Together, our findings provided a novel insight into abdominal fat content discrepancy between the fat and lean chicken lines.
Fermented cottonseed meal improves production performance and reduces fat deposition in broilers chicken