Evaluation of TA10 Broth for Recovery of Listeria monocytogenes from Ground Beef

2017 ◽  
Vol 100 (2) ◽  
pp. 470-473
Author(s):  
Naoko Kamisaki-Horikoshi ◽  
Yukio Okada ◽  
Kazuko Takeshita ◽  
Makoto Takada ◽  
Shinichi Kawamoto ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Ground Beef ◽  
Culture Method ◽  
Most Probable Number ◽  
Salmonella Spp ◽  
Probable Number ◽  
Enrichment Broth ◽  
Conventional Culture ◽  
Significant Difference ◽  
Peptone Water

Abstract In 2009, the enrichment broth TA10 was released for simultaneous recovery of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7. This medium was compared with other Salmonella enrichment broths [lactose (LAC) broth, buffered peptone water (BPW), and universal pre-enrichment (UP) broth] for the recovery of heat- and freeze-injured Salmonella spp. in beef by the conventional culture method. There was a significant difference between TA10 and LAC enrichment broths for detecting injured Salmonella spp. In this study, the International Organization for Standardization Listeria pre-enrichment broth (Half-Fraser/Fraser) was compared with TA10 broth for the recovery of L. monocytogenes from ground beef. Ground beef samples were contaminated with single Listeria serovars at levels of 0.096 to 0.001 most probable number/g. Twenty 25 g test portions of the contaminated ground beef were pre-enriched in each broth, and the ISO-11290-1 Listeria official isolation protocol was used thereafter. There was a significant difference between TA10 broth (48 h) and Half-Fraser/Fraser broth (72 h) in the recovery of L. monocytogenes. In addition, the incubation time for TA10 broth was shorter than for Half-Fraser/Fraser broth. The results indicate that TA10 broth should be used instead of Half-Fraser/Fraser broth for analysis of beef that may be contaminated with very low levels of L. monocytogenes.

scholarly journals Evaluation of TA10 Broth for Recovery of Heat- and Freeze-Injured Salmonella from Beef

2011 ◽  
Vol 94 (3) ◽  
pp. 857-862 ◽  
Author(s):  
Naoko Kamisaki -Horikoshi ◽  
Yukio Okada ◽  
Kazuko Takeshita ◽  
Takashi Sameshima ◽  
Susumu Kawasaki ◽  
...  
Keyword(s):  
Culture Method ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Probable Number ◽  
Enrichment Broth ◽  
Significant Difference ◽  
Peptone Water ◽  
Salmonella Serovars ◽  
Contamination Levels

Abstract The Bacteriological Analytical Manual (BAM) Salmonella pre-enrichment broth [lactose (LAC) broth], buffered peptone water, and universal pre-enrichment (UP) broth were compared with TA10 broth, developed in our laboratory, for recovery of heat- and freeze-injured Salmonella (55°C for 2–20 min and –20°C for 2 months, respectively) from beef. Beef samples were contaminated with single Salmonella serovars, and contamination levels of 0.44 to <0.001 most probable number (MPN)/g and 0.74 to 0.14 MPN/g were used for heat- and freezing-induced injury studies, respectively. Twenty test portions (25 g) of the contaminated beef were pre-enriched in each broth, and the BAM Salmonella culture method was used thereafter. There was a significant difference (χ2 = 7.73) in recovery of heat-injured Salmonella between TA10 broth and LAC broth, 189 (67.5%) versus 156 (55.7%) positive samples, respectively, determined by plating onto selective agars and identification by biochemical tests. For the recovery of freeze-injured Salmonella, there was a significant difference (χ2 = 24.7) between TA10 and LAC broth, 189 (72.7%) versus 133 (51.2%) positive samples, respectively. TA10 broth was more effective than LAC broth and UP broth for recovery of freeze-injured Salmonella. The results indicate that TA10 broth should be used instead of LAC broth for testing of beef that may be contaminated with heat- and freeze-injured Salmonella spp.

Relative Effectiveness of Selenite Cystine Broth, Tetrathionate Broth, and Rappaport-Vassiliadis Medium for the Recovery of Salmonella from Raw Flesh and Other Highly Contaminated Foods: Precollaborative Study

1995 ◽  
Vol 78 (2) ◽  
pp. 375-380 ◽  
Author(s):  
Geraldine A June ◽  
Patricia S Sherrod ◽  
Thomas S Hammack ◽  
R Miguel Amaguana ◽  
Wallace H Andrews
Keyword(s):  
Relative Effectiveness ◽  
Ground Beef ◽  
Most Probable Number ◽  
Salmonella Spp ◽  
Probable Number ◽  
Pork Sausage ◽  
Selective Enrichment

Abstract The effectiveness of selenite cystine (SC) broth, tetrathionate (TT) broth, and Rappaport-Vassiliadis (RV) medium for recovery of Salmonella spp. from 8 highly contaminated foods was determined. RV medium prepared from individual ingredients and incubated at 42° and 43°C was compared with 2 commercial (Difco and Oxoid) media incubated at 42°C. Naturally and artificially contaminated foods were tested under 2 protocols. For Protocol 1, each food was preenriched in the appropriate medium. After incubation, serial 10 fold dilutions of the preenriched foods were inoculated into selective enrichment media and incubated at 35°, 42°, or 43°C. Effectiveness of these conditions was evaluated by most probable number determination of Salmonella spp. recovered. Productivity of selective enrichments did not differ significantly with this protocol, except that with Oxoid RV medium the number of Salmonella cells recovered from most of the foods was significantly reduced. For Protocol 2, twenty 25 g test portions from artificially inoculated foods were examined qualitatively for Salmonella spp. The effectiveness of the broth/temperature combinations was determined by the number of positive tests under each condition. RV medium prepared from individual ingredients and TT broth incubated at 43°C yielded significantly more Salmonella-positive tests from frog legs and lettuce than did SC and TT broths incubated at 35°C or commercial RV medium incubated at 42°C. With pork sausage and ground beef, significantly fewer Salmonella-positive tests were found with Oxoid RV medium incubated at 42°C and SC incubated at 35°C than from other selective enrichments. With chicken, fewer Salmonella-positive tests were found from SC and TT broths incubated at 35°C and Oxoid RV medium incubated at 42°C than from other selective enrichments. There were no significant differences among selective enrichments in the recovery of Salmonella spp. from the remaining foods. Overall, RV prepared from individual ingredients and incubated at 42°C yielded the highest number of Salmonella-posWive tests.

Miniaturized Most Probable Number and Enrichment Serology Technique for the Enumeration of Salmonella spp. on Poultry Carcasses

1997 ◽  
Vol 60 (11) ◽  
pp. 1306-1311 ◽  
Author(s):  
FLORENCE S. HUMBERT ◽  
GILLES SALVAT ◽  
FRANÇOISE LALANDE ◽  
PIERRE COLIN
Keyword(s):  
Analytical Approach ◽  
Analytical Procedure ◽  
Cost Effective ◽  
Test Material ◽  
Most Probable Number ◽  
Salmonella Spp ◽  
Probable Number ◽  
Chicken Skin ◽  
Peptone Water ◽  
Skin Samples

The ability of two 8-tube most probable number (MPN) techniques to quantitatively recover Salmonella spp. from 26 fresh, naturally contaminated chicken skin samples was compared. Individual macerated skin samples were tested in parallel using a traditional (tMPN) and a miniaturized (mMPN) analytical procedure. In the tMPN assay, replicate aqueous portions from each macerated sample were preenriched individually in buffered peptone water, selectively enriched in Müller-Kauffmann tetrathionate brilliant green broth (MKTBG), and plated on Rambach agar. Each MKTBG was also postenriched in M Broth, and the resulting postenrichment culture screened for the presence of Salmonella cells by enrichment serology (ES). Although a similar analytical approach was used in the mMPN assay, it differed from the tMPN in the use of smaller test volumes dispensed in microplates, and on a sedimented portion of skin macerate as test material. Of the 26 Salmonella-contaminated samples examined in this study, the tMPN coupled to Rambach agar or ES identified 23 and 24 positive samples, respectively. Under homologous conditions, the mMPN detected all 26 positive Salmonella contaminated samples. The most probable numbers in 100-g skin samples analyzed by the tMPN ranged from 18/100 g to 9,530,000/100 g with a median value of 570/100 g. Levels of contamination by the mMPN procedure ranged from 90/100 g to 556,000/100 g with a median value of 1,200/100 g. Statistical analysis of experimental data underlined the equivalence of the tMPN and the mMPN procedures and nonequivalence of the Rambach plating and ES conditions. It is suggested that the microplate mMPN coupled to ES offers a reliable and more cost-effective analytical approach for the quantitative recovery of Salmonella on broiler carcasses.

Effect of Trisodium Phosphate on Campylobacter Attached to Post-Chill Chicken Carcasses

1994 ◽  
Vol 57 (4) ◽  
pp. 324-326 ◽  
Author(s):  
MICHAEL F. SLAVIK ◽  
JEONG-WEON KIM ◽  
MICHAEL D. PHARR ◽  
DENNIS P. RABEN ◽  
SONIA TSAI ◽  
...  
Keyword(s):  
Culture Method ◽  
Trisodium Phosphate ◽  
Most Probable Number ◽  
Log P ◽  
Probable Number ◽  
Conventional Culture ◽  
And Control ◽  
Conventional Culture Method

Trisodium phosphate (TSP) was evaluated as a means to reduce Campylobacter on chicken carcasses. Post-chill chicken carcasses were dipped into a 10% TSP solution at 50°C for 15 s. After storing the TSP-treated carcasses for 0, 1 or 6 days at 4°C, the carcasses were subjected to the recovery of Campylobacter. The incidence and reduction of Campylobacter attached to the carcasses were measured using a nitrocellulose (NC) membrane lift, conventional culture method, and a most probable number (MPN) technique. In trials 1 and 2, the incidence of Campylobacter was measured. For 1 day-stored groups, Campylobacter was present on 96 and 100% of control carcasses and present on 24 and 28% of TSP-treated carcasses as measured by NC membrane lift method. The reduction was less (4 to 36%) when measured by culture method. For carcasses immediately subjected for the recovery of cells after treatment, there was no difference between TSP-treated and control carcasses by either NC membrane or culture method. In trial 3, the reduction levels of Campylobacter were quantified by using a MPN method. The levels of Campylobacter on carcasses were decreased by 1.5 and 1.2 logs in 1- and 6-day stored, TSP-treated carcasses, respectively (p < 0.05). However, TSP treatment at 10°C reduced the level of Campylobacter only by 0.16 log (p > 0.10).

Detection of Listeria monocytogenes in Commercially Broken Unpasteurized Liquid Egg in Japan

2009 ◽  
Vol 72 (1) ◽  
pp. 178-181 ◽  
Author(s):  
MIHO OHKOCHI ◽  
MIYUKI NAKAZAWA ◽  
NOBUHIRO SASHIHARA
Keyword(s):  
Listeria Monocytogenes ◽  
Most Probable Number ◽  
Contamination Rate ◽  
Detection Rates ◽  
Probable Number ◽  
Significant Difference ◽  
Liquid Whole Egg ◽  
Contamination Levels ◽  
Livestock Products ◽  
Good Agreement

Unpasteurized liquid whole-egg samples were collected from six and seven commercial establishments across Japan in 1993 and 1994 and in 2005, respectively. The samples were tested for the presence of Listeria spp. and Listeria monocytogenes. Detection rates of the Listeria spp. were greatly different among the egg-breaking facilities, with a range of 8 to 55%. There was no significant difference in the contamination rate between the samples from 1993 and 1994 and from 2005. L. monocytogenes was isolated from 2 of 487 (0.4%) samples in 1993 and 1994, and 2 of 316 (0.6%) samples in 2005. These detection rates are lower than known detection rates of other livestock products (5.1 to 42%). In 2005, the L. monocytogenes–positive samples were quantified by a most-probable-number counting experiment, and the contamination levels were below 7.5 organisms per 25 g of sample. D55°C-values of 0.59 to 4.08/min were determined for L. monocytogenes isolated in this study. This heat tolerance is in a good agreement with past reports, and slightly higher than that of Salmonella. This study suggests that the legal pasteurization condition of liquid egg is sufficient to ensure microbial safety against L. monocytogenes because the contamination rate and its level are considerably low.

scholarly journals Rapid Detection and Identification Systems for the Microbiological Assessment of Processed Soy Foods: A Review

2020 ◽  
Vol 9 (5) ◽  
pp. 22
Author(s):  
Mitsuru Katase ◽  
Kazunobu Tsumura
Keyword(s):  
High Throughput ◽  
Culture Method ◽  
Detection Methods ◽  
Most Probable Number ◽  
Filter Method ◽  
Probable Number ◽  
Soy Foods ◽  
Flight Mass Spectrometry ◽  
Conventional Culture ◽  
Detection And Identification

Plant-based diets are gaining interest in promoting physical and environmental health worldwide. The widely growing consumption of processed soy foods results in an increased demand for safe and high quality soy foods. Many of the rapid bacterial detection methods currently available are inhibited by components in the food matrixes. In recent years, high-throughput devices have been developed, which aid in the enumeration and evaluation of microorganisms in processed soy foods (automated fluorescent filter method, high-throughput identification using matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and automated most probable number method). These methods are more rapid and convenient compared to the conventional culture method. This review discusses alternate reliable methods for the microbiological assessment of processed soy foods, which guarantees the safety of the food delivered for consumption.

Comparison of Automated BAX PCR and Standard Culture Methods for Detection of Listeria monocytogenes in Blue Crabmeat (Callinectus sapidus) and Blue Crab Processing Plants

2011 ◽  
Vol 74 (11) ◽  
pp. 1930-1933 ◽  
Author(s):  
SIVARANJANI PAGADALA ◽  
SALINA PARVEEN ◽  
JURGEN G. SCHWARZ ◽  
THOMAS RIPPEN ◽  
JOHN B. LUCHANSKY
Keyword(s):  
Listeria Monocytogenes ◽  
Blue Crab ◽  
Environmental Samples ◽  
Culture Method ◽  
Culture Methods ◽  
Enrichment Broth ◽  
Significant Difference ◽  
Processing Plants ◽  
Standard Culture

This study compared the automated BAX PCR with the standard culture method (SCM) to detect Listeria monocytogenes in blue crab processing plants. Raw crabs, crabmeat, and environmental sponge samples were collected monthly from seven processing plants during the plant operating season, May through November 2006. For detection of L. monocytogenes in raw crabs and crabmeat, enrichment was performed in Listeria enrichment broth, whereas for environmental samples, demi-Fraser broth was used, and then plating on both Oxford agar and L. monocytogenes plating medium was done. Enriched samples were also analyzed by BAX PCR. A total of 960 samples were examined; 59 were positive by BAX PCR and 43 by SCM. Overall, there was no significant difference (P ≤0.05) between the methods for detecting the presence of L. monocytogenes in samples collected from crab processing plants. Twenty-two and 18 raw crab samples were positive for L. monocytogenes by SCM and BAX PCR, respectively. Twenty and 32 environmental samples were positive for L. monocytogenes by SCM and BAX PCR, respectively, whereas only one and nine finished products were positive. The sensitivities of BAX PCR for detecting L. monocytogenes in raw crabs, crabmeat, and environmental samples were 59.1, 100, and 60%, respectively. The results of this study indicate that BAX PCR is as sensitive as SCM for detecting L. monocytogenes in crabmeat, but more sensitive than SCM for detecting this bacterium in raw crabs and environmental samples.

Evaluation of the Listertest™ Method for Quantitation of Listeria monocytogenes in Seafoods

1997 ◽  
Vol 60 (4) ◽  
pp. 424-425 ◽  
Author(s):  
SUSAN A. McCARTHY
Keyword(s):  
Listeria Monocytogenes ◽  
Salmo Salar ◽  
Drug Administration ◽  
Most Probable Number ◽  
Probable Number ◽  
Immunomagnetic Capture ◽  
Smoked Salmon ◽  
Significant Difference ◽  
Seafood Products ◽  
The U.S

The U.S. Food and Drug Administration most probable number (MPN) method and the Listertest™ (immunomagnetic capture) were used to enumerate Listeria monocytogenes on laboratory-inoculated cooked crabmeat (Callinectus sapidus) and cold-smoked salmon (Salmo salar). The products were inoculated with <1.0 to 4.0 log CFU of L. monocytogenes per g and analyzed immediately. In the analyses of crabmeat, the results obtained by the MPN method were significantly lower (P < 0.05) than counts produced by the Listertest™. There was no significant difference (P < 0.05) between results produced by MPN and the Listertest™ in analyses of smoked salmon. The methods had the same variance in the quantitation of L. monocytogenes in these seafood products.

Use of the Chromogenic Substrate 5-Bromo-4-Chloro-3-Indolyl-B-D-Glucuronide (X-GLUC) for Enumerating Escherichia coli in 24 H from Ground Beef

1990 ◽  
Vol 53 (6) ◽  
pp. 508-510 ◽  
Author(s):  
LAWRENCE RESTAINO ◽  
ELON W. FRAMPTON ◽  
RICHARD H. LYON
Keyword(s):  
Escherichia Coli ◽  
Ground Beef ◽  
Most Probable Number ◽  
Chromogenic Substrate ◽  
Probable Number ◽  
E Coli ◽  
Glucuronidase Activity ◽  
Significant Difference ◽  
Plating Method ◽  
Positive Linear Correlation

A 24-h direct plating method for Escherichia coli using the chromogenic substrate 5-bromo-4-chloro-3-indolyl-B-D-glucuronide (X-GLUC) incorporated into a Peptone-tergitol agar base (PTX) was compared with the standard 3-tube Most Probable Number (MPN) method on 50 naturally contaminated ground beef samples. A paired-comparisons t-test showed no significant difference between the two methods. A positive linear correlation between the two methods was observed over the entire range of values. Ninety-seven percent of the positive colonies (blue colonies) on PTX agar were indentified as E. coli, whereas no atypical colonies (nonblue) were characterized as such. Thus, a simple and reliable enumeration of E. coli can be made within 24 h using the X-GLUC substrate in a selective agar as an indicator of B-glucuronidase activity.

Comparison of β-Glucuronidase and Indole-Based Direct Plating Methods for Enumerating Escherichia coli in Artificially Inoculated Ground Meats

1990 ◽  
Vol 53 (11) ◽  
pp. 933-935 ◽  
Author(s):  
ELON W. FRAMPTON ◽  
LAWRENCE RESTAINO ◽  
NANCY BLASZKO
Keyword(s):  
Escherichia Coli ◽  
Ground Beef ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Glucuronidase Activity ◽  
Plate Count Agar ◽  
Significant Difference ◽  
Indole Production

Peptone tergitol glucuronide (PTG) agar containing 4-methylumbelliferyl-β-D glucuronide (MUG) (for β-glucuronidase activity), the Holbrook, Anderson, Baird-Parker (HABP) method (for detecting indole production), and the standard 3-tube most probable number (MPN) method were compared with plate count agar (PCA) for enumerating three strains of unstressed Escherichia coli artificially inoculated into ground beef and chicken at 1–6 × 106 cells/g. No significant difference (P>0.05) was determined between PTG agar and PCA in the recovery of E. coli. The MPN method enumerated a significantly greater (P<0.05) number of E. coli cells than PCA. Compared with PCA, the HABP method recovered a significantly lower (P<0.05) number of E. coli cells from chicken, whereas no significant difference (P>0.05) was obtained with ground beef. When combining all data from chicken and beef, the recovery of E. coli cells by the HABP method was also significantly lower (P<0.05). Overall, based on the enumeration of E. coli on PCA, the HABP method, PTG agar, and MPN method recovered 57, 102, and 144%, respectively.

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