Sustainable Eco-Friendly Ultra-High-Performance Liquid Chromatographic Method for Simultaneous Determination of Caffeine and Theobromine in Commercial Teas: Evaluation of Greenness Profile Using NEMI and Eco-Scale Assessment Tools

2018 ◽  
Vol 101 (6) ◽  
pp. 1781-1787 ◽  
Author(s):  
Heba Shaaban ◽  
Ahmed Mostafa
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Method Development ◽  
High Performance Liquid Chromatographic ◽  
Assessment Tools ◽  
Chromatography Method ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

Abstract Background: Green analytical chemistry (GAC) aims to eliminate or minimize the amount of hazardous solvents consumed and generated daily worldwide. Considering the environmental impact of all analytical procedures and replacing the polluting methodologies with clean ones is of a paramount interest. Objective: This work aims to develop and validate a sustainable, fast, and economic ultrahigh-performance liquid chromatography method for simultaneous determination of methylxanthines in commercial tea samples as well as to evaluate the greenness profile of the proposed method using two greenness assessment tools: National Environmental Methods Index (NEMI) and analytical Eco-scale. Methods: The method was designed based on applying GAC principles in method development. The green chromatography approach was applied by using benign mobile phases. The chromatographic separation was optimized to minimize sample preparation, achieve short analysis time with low solvent consumption, and minimize waste generation. Results: All the studied analytes were separated in only 1.7 min. The detection limits of the studied analytes ranged from 0.015 to 0.03 mg/L, while LOQs were in the range of 0.05 to 0.1 μg/L with UV detection. The proposed method neither uses nor generates harmful chemicals, it passes the four quadrants of the NEMI greenness profile, and it has a high Eco-scale score. Conclusions: Compared with the reported methods, the proposed method is greener, more economical, and faster; therefore, it can be used as a green alternative to the existing conventional methods for routine analysis of the studied analytes without harming the environment.

scholarly journals Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma

2013 ◽  
Vol 9 (2) ◽  
pp. 26-29 ◽  
Author(s):  
AK Hemanth Kumar ◽  
V Sudha ◽  
Geetha Ramachandran
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Pharmacokinetic Studies ◽  
Chromatography Method ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

A high performance liquid chromatographic method for determination of rifabutin in human plasma was  developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the  supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm.  The assay was specific for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation  of intra- and inter-day assays was lower than 10%. The method was able to remove interfering materials  in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay  can be used for pharmacokinetic studies of rifabutin. SAARC Journal of Tuberculosis, Lung Diseases & HIV/AIDS; 2012; IX(2) 26-29 DOI: http://dx.doi.org/10.3126/saarctb.v9i2.7975

RAPID HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF RALTEGRAVIR IN HUMAN SALIVA

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (09) ◽  
pp. 68-73
Author(s):  
K Vijaya Sri ◽  
M. Shiva Kumar ◽  
M. A. Madhuri ◽  
Suresha K. ◽  
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
High Performance Liquid Chromatographic ◽  
Human Saliva ◽  
Hplc Method ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

In this study, a high-performance liquid chromatographic method (HPLC) was developed, validated and applied for the determination of raltegravir in biological sample like saliva. Liquid- liquid extraction was performed for isolation of the drug and elimination of saliva interferences. Samples of saliva was extracted with 50µL of ortho phosphoric acid and 3ml of methanol was added and spiked with raltegravir. The chromatographic separation was performed on Agilent Eclipse C18 (100 mm × 4.6 mm, 3.5µm) column, by using 80:20 v/v acetonitrile: water as a mobile phase under isocratic conditions at a flow rate of 1.0 mL/min for UV detection at 240 nm. Retention time of raltegravir was found to be 1.030 min. Linearity was found to be in the range of 25-1000 ng/mL with regression equation y = 13864x + 40495 and correlation coefficient 0.999. The low % RSD value indicates the method is accurate and precise. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.76 and 2.28 ng/mL, respectively. It can be concluded that this validated HPLC method is easy, precise, accurate, sensitive and selective for determination of raltegravir in saliva.

Method Development and Validation of Propofol by Reverse Phase HPLC and its Estimation in Commercial Formulation

2021 ◽  
pp. 3139-3142
Author(s):  
Kuntal Mukherjee ◽  
S. T. Narenderan ◽  
B. Babu ◽  
Survi Mishra ◽  
S. N. Meyyanathan
Keyword(s):  
High Performance ◽  
Method Development ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reverse Phase Hplc ◽  
Liquid Chromatographic Method ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Liquid Chromatographic

A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Propofol. The main focus of the method was to determine Propofol in solution form as well as in marketed formulation. Chromatographic separation was achieved on Inertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase consisting of methanol: water (85:15), with a flow rate of 1.0ml/min (UV detection at 270nm). Linearity was observed over the concentration range of 10-110µg/ml with a regression equation y=88048x + 44524 and having a regression value (R2) of 0.999. The LOD and LOQ values found to be 10ng and 100ng, respectively. No changes found in ruggedness and robustness studies. The percentage of recovery was found to be 95.25% to 101.81%. Validation studies revealed that the method was specific, accurate, precise, reliable, robust, reproducible and suitable for the quantitative analysis in its pharmaceutical formulations.

scholarly journals Isocratic High-Performance Liquid Chromatographic Method with Ultraviolet Detection for Simultaneous Determination of Levels of Voriconazole and Itraconazole and Its Hydroxy Metabolite in Human Serum

2005 ◽  
Vol 49 (8) ◽  
pp. 3569-3571 ◽  
Author(s):  
GholamAli Khoschsorur ◽  
Franz Fruehwirth ◽  
Sieglinde Zelzer
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Specific Method ◽  
Minimum Detectable Concentration ◽  
Detectable Concentration ◽  
One Step ◽  
Liquid Chromatographic

ABSTRACT A simple, specific method is presented for simultaneous determination of voriconazole and itraconazole and its metabolite, hydroxyitraconazole, in human serum using one-step liquid-liquid extraction and high-performance liquid chromatography. Linearity tests ranged from 0.1 to 8.0 μg/ml; the minimum detectable concentration was 0.03 μg/ml.

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