Authentication of Two Bio-Active Fish Oils by Qualitative Lipid Profiling Using Semi-Targeted Approach: An Exploratory Study

2020 ◽  
Vol 103 (1) ◽  
pp. 78-82
Author(s):  
Niladri S Chatterjee ◽  
Akanksha Singh ◽  
K V Vishnu ◽  
K K Ajeeshkumar ◽  
R Anandan ◽  
...  
Keyword(s):  
Ion Trap ◽  
Neutral Loss ◽  
Market Price ◽  
Fish Oils ◽  
Lipid Profiling ◽  
Shark Liver Oil ◽  
Sardine Oil ◽  
Lipid Species ◽  
Food Market ◽  
Food Commodities

Abstract Background: Fish oils, which are rich in health-promoting polyunsaturated fatty acids (PUFA), have emerged as promising functional foods in the global health and wellness food market. Their source regarding the fish type, season, and location of harvesting might influence the nutritional value of such bioactive oils and determine their market price. The differences in price among such oils often lead to economically motivated mislabeling and adulteration. Objective: In this study, our objective was to demonstrate how a qualitative targeted shotgun lipid profile workflow using an electrospray ionization–quadrupole-linear ion trap MS (QTrap) could differentiate fish oils originating from two different species. Methods: Five samples each of sardine (Sardinella longiceps) oil and shark (Echinorhinus brucus) liver oil were diluted to a concentration of 80 µg/mL in chloroform–methanol (1 + 2, v/v) with 5 mM ammonium acetate. These samples were directly infused into a QTrap MS. The data were acquired for 23 precursor ion and 4 neutral loss scan experiments in the positive ionization mode and compared. Results: We identified the following major lipid classes: cholesteryl ester, diacyl glycerol, triacylglycerol, monoalkyldiacylglycerol, and phophatydyl choline. The relative peak areas of the identified lipid species, when subjected to supervised multivariate analysis, could effectively distinguish the sardine oil and shark liver oil. Conclusions: The approach will be useful in establishing authenticity of fish oil and to support the regulatory agencies in dispute resolution. It can also be extended to establish authenticity in other agricultural and food commodities. Highlights: This paper reports a proof of concept for authenticating PUFA-rich fish supplements. A shotgun targeted lipidomics profile and chemometrics modeling successfully discriminated sardine oil and shark liver oil.

70 INCORPORATING MULTIPLE STAGES OF MASS SPECTROMETRY INTO LIPID PROFILING OF OOCYTES AND PRE-IMPLANTATION EMBRYOS

2015 ◽  
Vol 27 (1) ◽  
pp. 128
Author(s):  
V. Pirro ◽  
A. K. Jarmusch ◽  
C. R. Ferreira ◽  
A. F. González-Serrano ◽  
J. E. Hallett ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Degrees Of Freedom ◽  
Ion Trap ◽  
Neutral Loss ◽  
Chemical Information ◽  
Lipid Profiling ◽  
Rat Embryos ◽  
Sample Pooling ◽  
Product Ions ◽  
Multiple Stages

Lipid profiling by mass spectrometry (MS) is increasingly used for the analysis of oocytes, embryos, and other reproductive cells. This analytical approach has several advantages, such as simple preparation (no need to perform extraction or separation), low detection limits (no need of sample pooling), and detection of structurally intact and diverse lipids. Many degrees of freedom are ensured by MS techniques (e.g. with the adoption of diverse ionization sources, mass analyzers, data acquisition systems), and this broadens the classes of lipids that can be detected and identified. Tandem or high-resolution MS experiments are normally performed for chemical characterisation. However, the use of novel approaches is a constant need to obtain deeper structural insights into lipids of biological interest, resulting in an information-rich dataset. Here we propose the use of multiple stages of MS for lipid profiling, specifically MS/MS data domain (i.e. ion mapping) experiments, so that comprehensive structural and relationship information (i.e. classes) can be extracted from a dataset. Indeed, the data generated have 2 dimensions of mass (i.e. precursor and product ions) and one of ion intensity, resulting in a datacube structure. Cutting through the datacube in different ways allows the extrapolation of (i) chemical composition of specific compounds (i.e. product scans) and (ii) pattern recognition for compounds that share identical neutral or charged fragments loss (i.e. neutral loss and precursor scans, respectively). The global chemical information enclosed into the datacube can be also processed by means of multiway statistical analyses to chemically characterise cells and cellular compartments. Preliminary data have been acquired, and the development of statistical tools for data processing is ongoing. Bovine and rat embryos were used for the experiments and analysed by extraction spray ambient MS. Experiments were performed with a Thermo Finnigan LTQ linear ion trap. Dimethylformamide-acetonitrile (1 : 1 v/v) was used as spray solvent. The ion mapping experiment was configured to scan ions of mass-to-charge (m/z) ratio 700 to 900 and perform MS/MS every m/z 1.5 window with a collision energy of 25 (arbitrary units). Fragments were detected in the m/z range of 150 to 900. Chemical differences are present between bovine and rat embryos, of note are palmitic, oleic, and stearic acids. The application of ion mapping to characterise species-specific and developmental dynamics regarding lipid composition is currently under investigation.

Lipid Profiling of Synechococcus sp. PCC7002 and Two Related Strains by HPLC Coupled to ESI-(Ion Trap)-MS/MS

2011 ◽  
Vol 66 (3-4) ◽  
pp. 149-158 ◽  
Author(s):  
Olimpio Montero
Keyword(s):  
High Performance ◽  
Ion Trap ◽  
Fatty Acyl ◽  
Lipid Profiling ◽  
Lipid Species ◽  
Negative Ionization Mode ◽  
Ionization Mode ◽  
Synechococcus Sp ◽  
Negative Ionization ◽  
Fragmentation Patterns

The lipid profiles of Synechococcus sp. PCC7002 and two related 16S rDNA (99% identity) strains were established by a new method of high-performance liquid chromatography coupled to electrospray-mass spectrometry (HPLC-MS). Lipids were analysed in the positive and negative ionization mode, and fragmentation patterns are reported. No differences in the lipid profi le between the three strains could be observed, but the relative content of some species differed. Major lipid species were found to be 1-octadecatrienoyl- 2-hexadecanoyl-3-(6’-sulfo-α-D-quinovosyl)-sn-glycerol [SQDG (18:3/16:0)] and 1-octadecatrienoyl-2-hexadecenoyl-3-β-D-monogalactosyl-sn-glycerol [MGDG (18:3/16:1)]. Ten species of SQDG, six species of PG (phosphatidyl-glycerol), seven species of MGDG, and two species of DGDG (digalactosyl-diacyl-glycerol) were detected. A PG species (m/z 761) containing hydroxylinolenic acid or oxophytodienoic acid acyl ester (C18H32O3), and SQDG species containing C17:1 and C17:3 fatty acyl esters are reported for the first time in cyanobacteria. The method also allowed the separation of two pairs of closely related isobaric MGDG species (m/z 770 and m/z 772 in positive ionization)

scholarly journals Urinary Lipid Profiling for the Identification of Fabry Hemizygotes and Heterozygotes

2005 ◽  
Vol 51 (4) ◽  
pp. 688-694 ◽  
Author(s):  
Maria Fuller ◽  
Peter C Sharp ◽  
Tina Rozaklis ◽  
Phillip D Whitfield ◽  
David Blacklock ◽  
...  
Keyword(s):  
Lysosomal Storage Disorder ◽  
Control Group ◽  
Tandem Mass ◽  
Lysosomal Storage ◽  
Lysosomal Hydrolase ◽  
Lipid Profiling ◽  
Enzyme Replacement ◽  
Lipid Species ◽  
Patient Groups ◽  
Ceramide Trihexoside

Abstract Background: Fabry disease is an X-linked lysosomal storage disorder resulting from a deficiency of the lysosomal hydrolase, α-galactosidase, for which enzyme replacement therapy is now available. In this study, we aimed to identify Fabry heterozygotes not only for genetic counseling of families but because it is becoming increasingly obvious that many heterozygous (carrier) females are symptomatic and should be considered for treatment. Methods: We measured 29 individual lipid species, including ceramide, glucosylceramide, lactosylceramide, and ceramide trihexoside, in urine samples from Fabry hemizygotes and heterozygotes and from control individuals by electrospray ionization tandem mass spectrometry. Individual analyte species and analyte ratios were analyzed for their ability to differentiate the control and patient groups. Results: The Fabry hemizygotes had increased concentrations of the substrate for the deficient enzyme, ceramide trihexoside, as well as lactosylceramide and ceramide, along with decreased concentrations of both glucosylceramide and sphingomyelin. Ratios of these analytes improved differentiation between the control and Fabry groups, with the Fabry heterozygotes generally falling between the Fabry hemizygotes and the control group. Conclusions: These lipid profiles hold particular promise for the identification of Fabry individuals, may aid in the prediction of phenotype, and are potentially useful for the monitoring of therapy in patients receiving enzyme replacement.

THE EFFECT OF DIETARY MARINE FISH OILS ON THE SERUM CHOLESTEROL LEVELS IN HYPERCHOLESTEROLEMIC CHICKENS

1960 ◽  
Vol 38 (1) ◽  
pp. 19-24 ◽  
Author(s):  
J. D. Wood ◽  
Jacob Biely
Keyword(s):  
Marine Fish ◽  
Serum Cholesterol ◽  
Corn Oil ◽  
Cholesterol Concentration ◽  
Fish Oils ◽  
Shark Liver Oil ◽  
Serum Cholesterol Concentration ◽  
Cholesterol Levels ◽  
Clear Explanation ◽  
Basking Shark

An investigation was carried out into the effect of marine fish oils on the serum cholesterol levels in hypercholesterolemic chickens. The oils were fed at the 10% level in the diet. Lingcod liver oil and halibut liver oil prevented the hypercholesterolemic effect of supplementary cholesterol, whereas crude herring oil increased the hypercholesterolemia to the same extent as did corn oil and tallow. Ratfish liver oil, dogfish liver oil, and basking shark liver oil had less noticeable effects on the cholesterol levels. No clear explanation could be given for the observed behavior but there seemed to be a difference in the effects on serum cholesterol of liver oils from teleostei fish and from selachii fish, the former class of liver oils being much more potent in preventing the increase in serum cholesterol concentration in chickens caused by the addition of cholesterol to the diet.

THE EFFECT OF DIETARY MARINE FISH OILS ON THE SERUM CHOLESTEROL LEVELS IN HYPERCHOLESTEROLEMIC CHICKENS

1960 ◽  
Vol 38 (1) ◽  
pp. 19-24 ◽  
Author(s):  
J. D. Wood ◽  
Jacob Biely
Keyword(s):  
Marine Fish ◽  
Serum Cholesterol ◽  
Corn Oil ◽  
Cholesterol Concentration ◽  
Fish Oils ◽  
Shark Liver Oil ◽  
Serum Cholesterol Concentration ◽  
Cholesterol Levels ◽  
Clear Explanation ◽  
Basking Shark

An investigation was carried out into the effect of marine fish oils on the serum cholesterol levels in hypercholesterolemic chickens. The oils were fed at the 10% level in the diet. Lingcod liver oil and halibut liver oil prevented the hypercholesterolemic effect of supplementary cholesterol, whereas crude herring oil increased the hypercholesterolemia to the same extent as did corn oil and tallow. Ratfish liver oil, dogfish liver oil, and basking shark liver oil had less noticeable effects on the cholesterol levels. No clear explanation could be given for the observed behavior but there seemed to be a difference in the effects on serum cholesterol of liver oils from teleostei fish and from selachii fish, the former class of liver oils being much more potent in preventing the increase in serum cholesterol concentration in chickens caused by the addition of cholesterol to the diet.

A Neutral Loss Activation Method for Improved Phosphopeptide Sequence Analysis by Quadrupole Ion Trap Mass Spectrometry

2004 ◽  
Vol 76 (13) ◽  
pp. 3590-3598 ◽  
Author(s):  
Melanie J. Schroeder ◽  
Jeffrey Shabanowitz ◽  
Jae C. Schwartz ◽  
Donald F. Hunt ◽  
Joshua J. Coon
Keyword(s):  
Mass Spectrometry ◽  
Sequence Analysis ◽  
Ion Trap ◽  
Neutral Loss ◽  
Quadrupole Ion Trap ◽  
Activation Method ◽  
Ion Trap Mass Spectrometry

The lipid metabolome of kidney cancer.

2012 ◽  
Vol 30 (30_suppl) ◽  
pp. 16-16
Author(s):  
Saby George ◽  
Kyoung-Soo Choi ◽  
Jeff C. Miecznikowski ◽  
Roberto Pili ◽  
A. Latif Kazim
Keyword(s):  
Kidney Cancer ◽  
Treatment Strategies ◽  
Charge Ratio ◽  
P Value ◽  
Detailed Knowledge ◽  
Lipid Profiling ◽  
Butyl Ether ◽  
Bonferroni Adjustment ◽  
Lipid Species ◽  
And Control

16 Background: The commonest type of kidney cancer is CCRCC. Kidney cancer is known to accumulate lipids and a detailed knowledge of the lipid species present in these tumors could lead to a better understanding of the underlying aberrant metabolic pathways and suggest possible treatment strategies. We attempt to identify the lipidomic profile of CCRCC using a liquid chromatography MS-based approach (LC-MS). Methods: We utilized 6 fresh frozen representative samples of CCRCC and matching non-tumor areas of kidney from nephrectomy samples. Lipids and other non-polar cellular constituents were extracted from both CCRCC and control tissues by methyl-t-butyl ether /methanol. LC-MS based lipid profiling was performed on a Waters Q-ToF Premier MS coupled with Ultra Performance LC. The peak detection and alignment across all chromatograms were performed using the XCMS software (v 1.14.1, Scripps Center for Metabolomics). Statistical comparisons of the intensities of aligned peaks were performed using the XCMS-built-in Welch's t-test. The XCMS data was converted to log2 ratios (normal/tumor) in order to utilize the paired aspect of this data: each patient’s disease tissue was analyzed in conjunction with corresponding normal tissue. Significance was determined by controlling the family wise error rate (FWER) at 0.05 using a Bonferroni adjustment. All computations were performed using the R statistical software and the “limma” package. Results: The outcome of XCMS was converted to a table that contains fold change, p value and mass to charge ratio (m/z) for each peak, its corresponding retention time, and the integrated peak intensities from all samples. Controlling FWER at 0.05 using a Bonferroni scheme, we found eight statistically significant lipids. Preliminary attempts to identify the analytes included use of METLIN (Scripps Center for Metabolomics) and HMDB (Human metabolome database, Genome Alberta and Genome Canada) databases. The identified metabolites included phosphatidylcholines, cholesterol esters and triglycerides, as well as other lipid species. Conclusions: The lipid metabolomic profile varied significantly between CCRCC and control. Further studies are underway to confirm the identities and significance of the lipid species in detail.

Sign in / Sign up

Export Citation Format

Share Document