Determination of Organophosphorus and Carbamate Insecticides in Fresh Fruits and Vegetables by High-Performance Thin-Layer Chromatography-Multienzyme Inhibition Assay

2012 ◽  
Vol 95 (5) ◽  
pp. 1371-1377 ◽  
Author(s):  
Rami Akkad ◽  
Wolfgang Schwack
Keyword(s):  
High Performance ◽  
Fruits And Vegetables ◽  
Inhibition Assay ◽  
Hptlc Plate ◽  
Liver Esterase ◽  
Carbamate Insecticides ◽  
Maximum Residue Limits ◽  
Primary Secondary Amine ◽  
Layer Chromatography

Abstract HPTLC-enzyme inhibition assay was applied to different fruit and vegetable samples after individual spiking with organophosphate and carbamate pesticides at their maximum residue limits documented by the European Commission. Samples were extracted according to the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, including cleanup by primary secondary amine sorbent. Additional cleanup was performed on the HPTLC plate by a prechromatographic step to separate most coextracted matrix compounds from 20 different pesticides under study. With both rabbit liver esterase and cutinase from Fusarium solani pisi as enzyme sources, mean recoveries from apples, cucumbers, grapes, nectarines, plums, tomatoes, and lemons were in the ranges 86–109, 95–129, 96–114, and 90–111% for chlorpyrifos, paraoxon, parathion, and pirimicarb, respectively, with a mean RSD of 8.5% for all samples.

scholarly journals Fingerprint Study of Curcuma xanthorrhiza Rhizome by High Performance Thin Layer Chromatography (HPTLC)

2016 ◽  
Vol 1 (2) ◽  
pp. 9-14
Author(s):  
Tina Wikara ◽  
Anny Sulistiowaty ◽  
Sri Murhandini ◽  
Tepy Usia
Keyword(s):  
High Performance ◽  
Phytochemical Analysis ◽  
Hptlc Plate ◽  
Spot Detection ◽  
Ich Guidelines ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography ◽  
Chloroform Methanol

The rhizome of Curcuma xanthorrhiza Roxb is intensively used in Indonesia as traditional medicine. It is widely used for hepatoprotective and anti inflammatory activities. To ensure the quality of its extract, we have studied the fingerprint or phytochemical analysis. This research was aimed to produce a chromatogram profile of the rhizome by HPTLC. The HPTLC fingerprint chromatogram of C. xanthorrhiza rhizome was performed using HPTLC plate of silica gel 60 F254 as the stationary phase and chloroform-methanol (97:3) as the mobile phase. Spot detection was carried out by TLC photo documentary system at 254 and 366 nm and TLC scanner at 427 nm. The developed method was validated according to ICH guidelines by determination of specificity and precision. We found that the specifity and precission of the method were met the acceptance criteria. In conclusion, the developed method is valid and could be used for quality control and standardization of herbal medicine containing C. xanthorrhiza rhizome.

scholarly journals Evaluation of Some Pesticide Residues in Fruits import by High Performance Liquid Chromatography

2017 ◽  
Vol 27 (4) ◽  
Author(s):  
Lamees M. A. Salman ◽  
Iyad Abdul-Muhsan Ahmed
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pesticide Residues ◽  
High Performance ◽  
Fruits And Vegetables ◽  
Current Knowledge ◽  
Maximum Residue Limits ◽  
Exposure Risks ◽  
Core Mixture

Pesticide residues have been found in various fruits and vegetables.  This study collected 24 samples and reported a method based on High Performance Liquid Chromatography (HPLC). For determination of pesticide residues used in Some fruits which were collected from different markets of Baghdad city to make) 24)  samples from (peel , core, mixture) for each type of fruits markets as:  Oranges( Egypt , Africa), Pomegranate ( Egypt), Mango ( Kenya ), Pears (China),  Plum fruits ( Africa ) , Kiwi (Turkey ) .That detective of ( 5 ) different  pesticides (diazinon,  malathion, chlorpyrifos ,parathion  and cypermethrin). The results were detected of multi-residues of  pesticides on the fruit in ( peel , core , mixture ) may be in the limit of Maximum residue limits(MRL) or higher of it   . The pesticides detected that exceeding the limits are: cypermethrin  in Kiwi (peel, core and mixture)at(0.204,0.038, 0.537), in pomegranate detected in (peel and mixture) at (0.509,0.189) mg/kg , Diazinon detccted in Egyptian orange in (peel ,core and mixture) at (0.031,0.207, 0.099) mg/kg . Malathion and Parathion was not detected at any type of fruits and  that results was compared with codex of FAO/WHO(2013). That data is important to monitor residues in food and to fill gaps in current knowledge would be helpful in assessing human exposure risks from ingestion of contaminated Fruits Imported to our country.

scholarly journals Evaluation of Some Pesticide Residues in Fruits import by High Performance Liquid Chromatography

2017 ◽  
Vol 27 (4) ◽  
Author(s):  
Lamees Munef Abdul-Latef ◽  
Iyad Abdul-Muhsan Ahmed
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pesticide Residues ◽  
High Performance ◽  
Fruits And Vegetables ◽  
Current Knowledge ◽  
Maximum Residue Limits ◽  
Exposure Risks ◽  
Core Mixture

Pesticide residues have been found in various fruits and vegetables.  This studycollected 24 samples and reported a method based on High Performance Liquid Chromatography (HPLC). For determination of pesticide residues used in Some fruits which were collected from different markets of Baghdad city to make) 24) samples from (peel , core, mixture) for each type of fruits markets as:  Oranges( Egypt , Africa), Pomegranate ( Egypt), Mango ( Kenya ), Pears (China),  Plum fruits ( Africa ) , Kiwi (Turkey ) .That detective of ( 5 ) different  pesticides (diazinon,  malathion, chlorpyrifos ,parathion  and cypermethrin). The results were detected of multi-residues of  pesticides on the fruit in ( peel , core , mixture ) may be in the limit of Maximum residue limits(MRL) or higher of it   . The pesticides detected that exceeding the limits are: cypermethrin  in Kiwi (peel, core and mixture)at(0.204,0.038, 0.537), in pomegranate detected in (peel and mixture) at (0.509,0.189) mg/kg , Diazinondetccted in Egyptian orange in (peel ,core and mixture) at (0.031,0.207, 0.099) mg/kg . Malathion and Parathion was not detected at any type of fruits and  that results was compared with codex of FAO/WHO(2013). That data is important to monitor residues in food and to fill gaps in current knowledge would be helpful in assessing human exposure risks from ingestion of contaminated Fruits Imported to our country .

scholarly journals Fingerprint Study of Curcuma xanthorrhiza Rhizome by High Performance Thin Layer Chromatography (HPTLC)

2016 ◽  
Vol 1 (2) ◽  
pp. 9-14 ◽  
Author(s):  
Tina Wikara ◽  
Anny Sulistiowaty ◽  
Sri Murhandini ◽  
Tepy Usia
Keyword(s):  
High Performance ◽  
Phytochemical Analysis ◽  
Hptlc Plate ◽  
Spot Detection ◽  
Ich Guidelines ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography ◽  
Chloroform Methanol

The rhizome of Curcuma xanthorrhiza Roxb is intensively used in Indonesia as traditional medicine. It is widely used for hepatoprotective and anti inflammatory activities. To ensure the quality of its extract, we have studied the fingerprint or phytochemical analysis. This research was aimed to produce a chromatogram profile of the rhizome by HPTLC. The HPTLC fingerprint chromatogram of C. xanthorrhiza rhizome was performed using HPTLC plate of silica gel 60 F254 as the stationary phase and chloroform-methanol (97:3) as the mobile phase. Spot detection was carried out by TLC photo documentary system at 254 and 366 nm and TLC scanner at 427 nm. The developed method was validated according to ICH guidelines by determination of specificity and precision. We found that the specifity and precission of the method were met the acceptance criteria. In conclusion, the developed method is valid and could be used for quality control and standardization of herbal medicine containing C. xanthorrhiza rhizome.

Recent Applications of High Performance Thin Layer Chromatography and Derivative Spectrophotometry in Pharmaceutical Analysis

2020 ◽  
Vol 16 (6) ◽  
pp. 671-689
Author(s):  
Marcin Gackowski ◽  
Marcin Koba ◽  
Katarzyna Mądra-Gackowska ◽  
Piotr Kośliński ◽  
Stefan Kruszewski
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pharmaceutical Analysis ◽  
High Performance ◽  
Degradation Products ◽  
Derivative Spectrophotometry ◽  
Accuracy And Precision ◽  
Post Marketing ◽  
Layer Chromatography

At present, no one can imagine drug development, marketing and post-marketing without rigorous quality control at each stage. Only modern, selective, accurate and precise analytical methods for determination of active compounds, their degradation products and stability studies are able to assure the appropriate amount and purity of drugs administered every day to millions of patients all over the world. For routine control of drugs simple, economic, rapid and reliable methods are desirable. The major focus of current scrutiny is placed on high-performance thin layer chromatography and derivative spectrophotometry methods, which fulfill routine drug estimation’s expectations [1-4]. The present paper reveals state-of-the-art and possible applications of those methods in pharmaceutical analysis between 2010 and 2018. The review shows advantages of high-performance thin layer chromatography and derivative spectrophotometry, including accuracy and precision comparable to more expensive and time-consuming methods as well as additional fields of possible applications, which contribute to resolving many analytical problems in everyday laboratory practice.

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