scholarly journals Evaluation of Factors Affecting European Plum (Prunus domestica L.) Genetic Transformation

10.5772/47071 ◽  
2011 ◽  
Author(s):  
Yuan Song ◽  
Fatih Ali ◽  
Farida Meerja ◽  
Xinhua Wang ◽  
Hugh A. L. Henry ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Prunus Domestica ◽  
Factors Affecting

FACTORS AFFECTING STORABILITY AND SHELF LIFE IN PLUMS (PRUNUS DOMESTICA L.)

2012 ◽  
pp. 197-203
Author(s):  
E. Vangdal ◽  
S. Flatland ◽  
I. Lunde Knutsen ◽  
H. Larsen
Keyword(s):  
Shelf Life ◽  
Prunus Domestica ◽  
Factors Affecting

scholarly journals A review on factors affecting the Agrobacterium -mediated genetic transformation in ornamental monocotyledonous geophytes

2015 ◽  
Vol 98 ◽  
pp. 37-44 ◽  
Author(s):  
M.J. Koetle ◽  
J.F. Finnie ◽  
E. Balázs ◽  
J. Van Staden
Keyword(s):  
Genetic Transformation ◽  
Factors Affecting

scholarly journals Advances in Agrobacterium-mediated plant transformation with enphasys on soybean

2008 ◽  
Vol 65 (1) ◽  
pp. 95-106 ◽  
Author(s):  
Paulo Celso de Mello-Farias ◽  
Ana Lúcia Soares Chaves
Keyword(s):  
Genetic Transformation ◽  
Plant Transformation ◽  
Animal Feed ◽  
Reporter Genes ◽  
Raw Material ◽  
Selectable Markers ◽  
Soybean Transformation ◽  
Factors Affecting ◽  
Comprehensive Discussion ◽  
Regeneration Systems

Soybean is one of humanity's major sources of plant protein. It is also very important for animal feed and as industrial raw material. Great advances have recently been achieved in its genetic transformation. This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes.

Factors affecting genetic transformation and shoot organogenesis of Bacopa monnieri (L.) Wettst

2012 ◽  
Vol 22 (4) ◽  
pp. 382-391 ◽  
Author(s):  
Diwakar Aggarwal ◽  
Neha Jaiswal ◽  
Anil Kumar ◽  
M. Sudhakara Reddy
Keyword(s):  
Genetic Transformation ◽  
Shoot Organogenesis ◽  
Bacopa Monnieri ◽  
Factors Affecting

The preparation of transforming DNA from Mycoplasma hominis strain Sprott tetr and quantitative studies of the factors affecting the genetic transformation of Mycoplasma salivarium strain S9 tets to tetracycline resistance

1980 ◽  
Vol 26 (9) ◽  
pp. 1147-1152 ◽  
Author(s):  
Anna M. Cerone-McLernon ◽  
Geoffrey Furness
Keyword(s):  
Genetic Transformation ◽  
Tetracycline Resistance ◽  
Mycoplasma Hominis ◽  
Dna Uptake ◽  
Optimum Conditions ◽  
Factors Affecting ◽  
Quantitative Studies ◽  
Viable Cells ◽  
Calf Thymus

DNA extracted by a standard method from Mycoplasma hominis Sprott, resistant to 100 μg tetracycline, permitted the quantitative genetic transformation of tetracycline-sensitive Mycoplasma salivarium to resistance. The yield was 1 μg DNA/109 cells. This DNA enabled determination of the optimum conditions for making M. salivarium competent with CaCl2 and for studying some factors affecting transformation. Mycoplasma salivarium was transformed to resistance to 10, 20, and 30 μg tetracycline but not to 40 μg. The optimum DNA concentration for transforming resistance to 10, 20, and30 μg tetracycline was the same, i.e., 50 μg DNA/108 viable cells. Treatment with DNase indicated that DNA uptake took 30 min. Competition between transforming DNA and DNA from calf thymus and M. salivarium tets inhibited transformation.

Genetic transformation of Prunus domestica L. using the hpt gene coding for hygromycin resistance as the selectable marker

2009 ◽  
Vol 119 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Lining Tian ◽  
Fatih Ali Canli ◽  
Xinhua Wang ◽  
Susan Sibbald
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Hygromycin Resistance ◽  
Prunus Domestica ◽  
Gene Coding

scholarly journals Olive (Olea europaea L.) Genetic Transformation: Current Status and Future Prospects

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 386
Author(s):  
Elena Palomo-Ríos ◽  
Isabel Narváez ◽  
Fernando Pliego-Alfaro ◽  
José A. Mercado
Keyword(s):  
Genetic Transformation ◽  
Olea Europaea ◽  
Fungal Resistance ◽  
Current Status ◽  
Biotic And Abiotic Stress ◽  
Juvenile Period ◽  
Factors Affecting ◽  
Olea Europaea L ◽  
Olea Europaéa

Olive (Olea europaea L.) is the most characteristic and important oil crop of the Mediterranean region. Traditional olive cultivation is based on few tens cultivars of ancient origin. To improve this crop, novel selections with higher tolerance to biotic and abiotic stress, adaptable to high-density planting systems and resilient to climate change are needed; however, breeding programs are hindered by the long juvenile period of this species and few improved genotypes have been released so far. Genetic transformation could be of great value, in the near future, to develop new varieties or rootstocks in a shorter time; in addition, it has currently become an essential tool for functional genomic studies. The recalcitrance of olive tissues to their in vitro manipulation has been the main bottleneck in the development of genetic transformation procedures in this species; however, some important traits such as fungal resistance, flowering or lipid composition have successfully been manipulated through the genetic transformation of somatic embryos of juvenile or adult origin, providing a proof of the potential role that this technology could have in olive improvement. However, the optimization of these protocols for explants of adult origin is a prerequisite to obtain useful materials for the olive industry. In this review, initially, factors affecting plant regeneration via somatic embryogenesis are discussed. Subsequently, the different transformation approaches explored in olive are reviewed. Finally, transgenic experiments with genes of interest undertaken to manipulate selected traits are discussed.

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