scholarly journals Molecular Tools for Gene Analysis in Fission Yeast

2020 ◽  
Author(s):  
Irma Pilar Herrera-Camacho ◽  
Lourdes Millán-Pérez-Peña ◽  
Francisca Sosa-Jurado ◽  
Nancy Martínez-Montiel ◽  
Rebeca Débora Martínez-Contreras ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Gene Analysis ◽  
Molecular Tools

scholarly journals Anniversary of the discovery/isolation of the yeast centromere by Clarke and Carbon

2015 ◽  
Vol 26 (9) ◽  
pp. 1575-1577 ◽  
Author(s):  
Kerry Bloom
Keyword(s):  
Fission Yeast ◽  
Dna Sequence ◽  
Budding Yeast ◽  
Sequence Specificity ◽  
Molecular Tools ◽  
Dna Sequence Specificity ◽  
Artificial Chromosomes

The first centromere was isolated 35 years ago by Louise Clarke and John Carbon from budding yeast. They embarked on their journey with rudimentary molecular tools (by today's standards) and little knowledge of the structure of a chromosome, much less the nature of a centromere. Their discovery opened up a new field, as centromeres have now been isolated from fungi and numerous plants and animals, including mammals. Budding yeast and several other fungi have small centromeres with short, well-defined sequences, known as point centromeres, whereas regional centromeres span several kilobases up to megabases and do not seem to have DNA sequence specificity. Centromeres are at the heart of artificial chromosomes, and we have seen the birth of synthetic centromeres in budding and fission yeast and mammals. The diversity in centromeres throughout phylogeny belie conserved functions that are only beginning to be understood.

scholarly journals Breeding of the Gull-billed Tern in the Sahara and an update on its distribution in Algeria

2020 ◽  
pp. 106-112
Author(s):  
Oussama Bouarakia ◽  
Christiane Denys ◽  
Violaine Nicolas ◽  
Touria Benazzou ◽  
Abdelaziz Benhoussa
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
Genetic Characterization ◽  
Taxonomic Status ◽  
The Body ◽  
Gene Analysis ◽  
Molecular Tools ◽  
Skull Measurements ◽  
Species Being

The taxonomic status of the gerbil Gerbillus amoenus in relation to Gerbillus nanus and the distribution range of these two species in Africa and/or Asia have long been debated and are not yet fully clarified. In our study, we identify two specimens of small gerbils that we captured in two localities of the south of Morocco, using morphometric and/or molecular tools. The body and skull measurements were not able to unambiguously discriminate between three closely related small gerbils (Gerbillus amoenus, Gerbillus nanus and Gerbillus henleyi). However, the cytochrome b gene analysis showed that our two specimens cluster unambiguously with haplotypes of G. amoenus. This represents the first genetic characterization of G. amoenus in Morocco. It confirms, based on mitochondrial DNA, that the previously described species living in Africa is indeed G. amoenus and not G. nanus, the latter species being present strictly in Asia.

Rapid and reliable preparation of total cell lysates from fission yeast

2006 ◽  
Author(s):  
Atsuko Shirai ◽  
Akihisa Matsuyama ◽  
Yoko Yashiroda ◽  
Ritsuko Arai ◽  
Minoru Yoshida
Keyword(s):  
Fission Yeast ◽  
Total Cell ◽  
Cell Lysates

Calcium sensing receptor gene: Analysis of polymorphism frequency

1997 ◽  
Vol 57 ◽  
pp. 122-125 ◽  
Author(s):  
L. A. Rubin ◽  
V. Peltekova ◽  
N. Janicic ◽  
C. C. Liew ◽  
D. Hwang ◽  
...  
Keyword(s):  
Receptor Gene ◽  
Gene Analysis ◽  
Calcium Sensing Receptor ◽  
Calcium Sensing ◽  
Calcium Sensing Receptor Gene
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