scholarly journals The Unusual Linear Plasmid Generating Systems of Prokaryotes

2020 ◽  
Author(s):  
Sophie E. Knott ◽  
Sarah A. Milsom ◽  
Paul J. Rothwell
Keyword(s):  
Linear Plasmid

scholarly journals Formation of linear plasmid multimers promoted by the phage lambda Red-system in lon mutants of Escherichia coli

1993 ◽  
Vol 139 (10) ◽  
pp. 2387-2397 ◽  
Author(s):  
y Mythili ◽  
K. Muniyappa
Keyword(s):  
Escherichia Coli ◽  
Linear Plasmid ◽  
Phage Lambda

Creation of a functional promoter by rearrangement in a Kluyveromyces lactis linear plasmid

Gene ◽  
1994 ◽  
Vol 147 (1) ◽  
pp. 125-129 ◽  
Author(s):  
Yu-Sheng Cong ◽  
Micheline Wésolowski-Louvel ◽  
Hiroshi Fukuhara
Keyword(s):  
Kluyveromyces Lactis ◽  
Linear Plasmid

scholarly journals CORRIGENDUM

Genetics ◽  
1987 ◽  
Vol 115 (3) ◽  
pp. 579-579
Keyword(s):  
Sister Chromatid ◽  
Recombination Event ◽  
Linear Plasmid ◽  
Wild Type ◽  
Spore Viability ◽  
Yeast Plasmid ◽  
Type Locus ◽  
Sister Chromatids ◽  
Chromosome Ii ◽  
Meiosis I

ABSTRACT In the paper by Jules O'Rear and Jasper Rine (Genetics  113: 517-529; July, 1986) entitled "Precocious meiotic centromere separation of a novel yeast chromosome," the authors described a gene conversion event between a linear yeast plasmid carrying a LYS2 gene and a mutant lys2 gene at the wild-type locus on chromosome II. When these yeasts were mated to wild-type yeast and the resulting diploids sporulated, linked markers on the linear plasmid showed unusual segregation and poor spore viability was observed. On the basis of these observations, we proposed that the recombination event between the linear plasmid and chromosome II had split chromosome II into two fragments, one of which carried the normal centromere of chromosome II (fragment IIa) and the other, a telocentric fragment (fragment IIb), carried the centromere present on the linear plasmid. Separation of the chromosomes from these cells on OFAGE gels verified that chromosome II had been split into two fragments. Furthermore, we proposed that the sister chromatids of the telocentric fragment (fragment IIb) separated precociously in meiosis I when complete chromosome II and fragment IIa were present. In discussions with colleagues, an alternative explanation arose in which a recombination event between a sister chromatid of fragment IIa and a sister chromatid of chromosome II would result in each chromosome II chromatid being joined to a fragment IIa chromatid at CEN2. The two daughter cells of meiosis I would therefore each receive one chromatid of fragment IIa and one chromatid of chromosome II. Segregation of the two sister chromatids of fragment IIb to one pole in meiosis I without precocious centromere separation would result in the observed tetrad classes. To distinguish between these two mechanisms, a centromere-linked marker was introduced into the cross between the strain containing the two fragments of chromosome II and a wild-type strain. Tetrad analysis of the resulting diploid is consistent with the recombination model for the poor spore viability and inconsistent with precocious centromere separation. We thank Drs. Eric Lambie, Michael Lichten and Tom Petes for helpful discussions.

Linear plasmids in Klebsiella and other Enterobacteriaceae

2021 ◽  
Author(s):  
Jane Hawkey ◽  
Hugh Cottingham ◽  
Alex Tokolyi ◽  
Ryan R Wick ◽  
Louise M Judd ◽  
...  
Keyword(s):  
Plasmid Stability ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Multidrug Resistant ◽  
Linear Plasmid ◽  
Extrachromosomal Dna ◽  
Linear Plasmids ◽  
Future Studies ◽  
And Function

Linear plasmids are extrachromosomal DNA that have been found in a small number of bacterial species. To date, the only linear plasmids described in the Enterobacteriaceae family belong to Salmonella, first found in Salmonella Typhi. Here, we describe a collection of 12 isolates of the Klebsiella pneumoniae species complex in which we identified linear plasmids. We used this collection to search public sequence databases and discovered an additional 74 linear plasmid sequences in a variety of Enterobacteriaceae species. Gene content analysis divided these plasmids into five distinct phylogroups, with very few genes shared across more than two phylogroups. The majority of linear plasmid-encoded genes are of unknown function, however each phylogroup carried its own unique toxin-antitoxin system and genes with homology to those encoding the ParAB plasmid stability system. Passage in vitro of the 12 linear plasmid-carrying Klebsiella isolates in our collection (which include representatives of all five phylogroups) indicated that these linear plasmids can be stably maintained, and our data suggest they can transmit between K. pneumoniae strains (including members of globally disseminated multidrug resistant clones) and also between diverse Enterobacteriaceae species. The linear plasmid sequences, and representative isolates harbouring them, are made available as a resource to facilitate future studies on the evolution and function of these novel plasmids.

Both open reading frames of the linear plasmid pMC3-2 from the ascomycete Morchella conica are transcribed in vivo

1992 ◽  
Vol 22 (6) ◽  
pp. 507-509 ◽  
Author(s):  
M. Rohe ◽  
F. Meinhardt
Keyword(s):  
Open Reading Frames ◽  
Linear Plasmid ◽  
Morchella Conica ◽  
Reading Frames

scholarly journals Contextual analysis of linear plasmid-like DNA of maize mitochondria: homology with virus sequences

1991 ◽  
Vol 7 (1) ◽  
pp. 30-37
Author(s):  
Yu. M. Konstantinov ◽  
M. V. Derenko ◽  
I. B. Rogozin
Keyword(s):  
Contextual Analysis ◽  
Linear Plasmid

scholarly journals Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres

1997 ◽  
Vol 26 (3) ◽  
pp. 581-596 ◽  
Author(s):  
Sherwood Casjens ◽  
Maria Murphy ◽  
Michael DeLange ◽  
Laura Sampson ◽  
René Van Vugt ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Lyme Disease ◽  
Linear Plasmid ◽  
Linear Chromosomes

scholarly journals The giant linear plasmid pHG207 from Rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini

1993 ◽  
Vol 139 (9) ◽  
pp. 2055-2065 ◽  
Author(s):  
J. Kalkus ◽  
C. Dorrie ◽  
D. Fischer ◽  
M. Reh ◽  
H. G. Schlegel
Keyword(s):  
Linear Plasmid ◽  
Rhodococcus Sp
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