Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR

A multiplex PCR method was developed for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in meat samples. DNA detection sensitivity for this method was 103 CFU/ml for each pathogen. When this protocol was used for the detection of each of the above pathogenic bacteria in spiked pork samples, 1 cell per 25 g of inoculated sample could be detected within 30 h. In the samples of naturally contaminated meat, Salmonella spp., L. monocytogenes, and E. coli O157:H7 were detected over the same time period. Excellent agreement was obtained for the results of multiplex PCR and the conventional culture method, which suggests that the multiplex PCR is a reliable and useful method for rapid screening of meat products for Salmonella spp., L. monocytogenes, and E. coli O157:H7 contamination.

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Simultaneous Detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in Low-fatted Milk by Multiplex PCR

Korean Journal for Food Science of Animal Resources ◽

10.5851/kosfa.2014.34.5.717 ◽

2014 ◽

Vol 34 (5) ◽

pp. 717-723 ◽

Cited By ~ 8

Author(s):

Ji-Hyun Kim ◽

Seong-Ryul Rhim ◽

Kee-Tae Kim ◽

Hyun-Dong Paik ◽

Joo-Yeon Lee

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Listeria Monocytogenes ◽

Bacillus Cereus ◽

Multiplex Pcr ◽

Simultaneous Detection ◽

Escherichia Coli O157 ◽

Salmonella Spp ◽

And Staphylococcus Aureus

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Development of the Multiplex PCR Detection Kit for Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7

Japan Agricultural Research Quarterly JARQ ◽

10.6090/jarq.45.77 ◽

2011 ◽

Vol 45 (1) ◽

pp. 77-81 ◽

Cited By ~ 8

Author(s):

Susumu KAWASAKI ◽

Pina M. FRATAMICO ◽

Naoko KAMISAKI-HORIKOSHI ◽

Yukio OKADA ◽

Kazuko TAKESHITA ◽

...

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Multiplex Pcr ◽

Pcr Detection ◽

Escherichia Coli O157 ◽

Salmonella Spp

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Survival of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp. on Catfish Fillets Exposed to Microwave Heating in a Continuous Mode

Journal of Food Science ◽

10.1111/j.1750-3841.2012.02817.x ◽

2012 ◽

Vol 77 (8) ◽

pp. E209-E214 ◽

Cited By ~ 7

Author(s):

Shiowshuh Sheen ◽

Lihan Huang ◽

Christopher Sommers

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Microwave Heating ◽

Escherichia Coli O157 ◽

Continuous Mode ◽

Salmonella Spp ◽

Catfish Fillets

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Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

Journal of Food Protection ◽

10.4315/0362-028x-62.4.325 ◽

1999 ◽

Vol 62 (4) ◽

pp. 325-328 ◽

Cited By ~ 38

Author(s):

C. LITTLE ◽

D. ROBERTS ◽

E. YOUNGS ◽

J. de LOUVOIS

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Microbiological Quality ◽

Escherichia Coli O157 ◽

Salmonella Spp ◽

Shigella Spp ◽

Natural Microflora ◽

Production Practices ◽

Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

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