Primary Plasmacytoma Arising in an Endocervical Polyp With Detection of Neoplastic Cells on Papanicolaou Test

2003 ◽  
Vol 127 (1) ◽  
pp. e28-e31
Author(s):  
Edgar G. Fischer ◽  
Thèrése J. Bocklage ◽  
Ian Rabinowitz ◽  
Harriet O. Smith ◽  
David S. Viswanatha
Keyword(s):  
Light Chain ◽  
Uterine Cervix ◽  
Plasma Cells ◽  
Female Genital Tract ◽  
Molecular Techniques ◽  
Chain Gene ◽  
Papanicolaou Test ◽  
Diagnostic Challenge ◽  
Chain Gene Rearrangement ◽  
Histopathologic Findings

Abstract Primary plasmacytomas of the female genital tract are extremely rare and present a substantial diagnostic challenge. Five cases morphologically representing plasmacytomas and localized to the uterine cervix have been reported previously; however, only 1 was shown to be monotypic for immunoglobulin light-chain expression. We report the case of a 37-year-old woman who had highly atypical plasma cells on her Papanicolaou test. A clinically detected endocervical polyp was removed and revealed a plasmacytoma, the diagnosis of which was substantiated by demonstrating monotypic λ-light-chain restriction and a clonal immunoglobulin heavy-chain gene rearrangement. The cytologic and histopathologic findings of plasmacytomas of the uterine cervix are discussed, including the utility of immunophenotypic and molecular techniques to confirm the neoplastic diagnosis.

scholarly journals Apolipoprotein C-II Deposition Amyloidosis: A Potential Misdiagnosis as Light Chain Amyloidosis

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Sadichhya Lohani ◽  
Emily Schuiteman ◽  
Lohit Garg ◽  
Dhiraj Yadav ◽  
Sami Zarouk
Keyword(s):  
Mass Spectrometry ◽  
Light Chain ◽  
Amyloid Fibrils ◽  
Plasma Cells ◽  
Laser Microdissection ◽  
Density Lipoprotein ◽  
Definitive Diagnosis ◽  
Diagnostic Challenge ◽  
Light Chain Amyloidosis ◽  
Apolipoprotein C

Hereditary amyloidoses are rare and pose a diagnostic challenge. We report a case of hereditary amyloidosis associated with apolipoprotein C-II deposition in a 61-year-old female presenting with renal failure and nephrotic syndrome misdiagnosed as light chain amyloidosis. Renal biopsy was consistent with amyloidosis on microscopy; however, immunofluorescence was inconclusive for the type of amyloid protein. Monoclonal gammopathy evaluation revealed kappa light chain. Bone marrow biopsy revealed minimal involvement with amyloidosis with kappa monotypic plasma cells on flow cytometry. She was started on chemotherapy for light chain amyloidosis. She was referred to the Mayo clinic where laser microdissection and liquid chromatography mass spectrometry detected high levels of apolipoprotein C-II, making a definitive diagnosis. Apolipoprotein C-II is a component of very low-density lipoprotein and aggregates in lipid-free conditions to form amyloid fibrils. The identification of apolipoprotein C-II as the cause of amyloidosis cannot be solely made with routine microscopy or immunofluorescence. Further evaluation of biopsy specimens with laser microdissection and mass spectrometry and DNA sequencing of exons should be done routinely in patients with amyloidoses for definitive diagnosis. Our case highlights the importance of determining the subtype of amyloidosis that is critical for avoiding unnecessary therapy such as chemotherapy.

scholarly journals Assessing the pathogenic potential of the V(D)J recombinase by interlocus immunoglobulin light-chain gene rearrangement.

1997 ◽  
Vol 17 (2) ◽  
pp. 887-894 ◽  
Author(s):  
S N Bailey ◽  
N Rosenberg
Keyword(s):  
Light Chain ◽  
Leukemia Virus ◽  
Chain Gene ◽  
Temperature Sensitive ◽  
Immunoglobulin Light Chain ◽  
Pathogenic Potential ◽  
Light Chain Gene ◽  
Large Numbers ◽  
Translocation Breakpoints ◽  
Chain Gene Rearrangement

Chromosomal translocations involving antigen receptor genes and oncogenes have been observed in several forms of lymphoid malignancy. Observations of their lymphocyte-restricted occurrence and a molecular analysis of some translocation breakpoints have suggested that some of these rearrangements are generated by V(D)J recombinase activity. However, a direct correlation between this activity and the generation of such rearrangements has never been established. In addition, because these aberrant rearrangements are usually detected only after a tumor has been formed, the frequency with which the recombinase machinery generates translocations has never been assessed directly. To approach these issues, immunoglobulin light-chain gene rearrangements were induced in pre-B cells transformed by temperature-sensitive mutants of Abelson murine leukemia virus and PCR was used to identify interlocus recombinants. Vlambda Jkappa and Vkappa Jlambda rearrangements as well as signal joints resulting from the recombination of Vlambda and Jkappa coding elements were recovered and were found to be similar in structure to conventional intrachromosomal joints. Because these products were detected only when the cells were undergoing active intralocus rearrangement, they provide direct evidence that translocations can be generated by the V(D)J recombinase machinery. Dilution analyses revealed that interlocus rearrangements occur about 1,000 times less frequently than conventional intralocus rearrangements. Considering the large numbers of lymphocytes generated throughout life, aberrant rearrangements generated by the V(D)J recombinase may be relatively common.

scholarly journals Immunoglobulin κ Light Chain Gene Rearrangement Is Impaired in Mice Deficient for DNA Polymerase Mu

Immunity ◽  
2003 ◽  
Vol 19 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Barbara Bertocci ◽  
Annie De Smet ◽  
Claudia Berek ◽  
Jean-Claude Weill ◽  
Claude-Agnès Reynaud
Keyword(s):  
Dna Polymerase ◽  
Light Chain ◽  
Gene Rearrangement ◽  
Chain Gene ◽  
Light Chain Gene ◽  
Chain Gene Rearrangement

Kappa Light Chain Gene Rearrangement in T-Cell Acute Lymphoblastic Leukemia

1990 ◽  
Vol 93 (4) ◽  
pp. 563-568 ◽  
Author(s):  
Curtis A. Hanson ◽  
Maran Thamilarasan ◽  
Charles W. Ross ◽  
Lloyd M. Stoolman ◽  
Bertram Schnitzer
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Light Chain ◽  
Gene Rearrangement ◽  
Lymphoblastic Leukemia ◽  
Chain Gene ◽  
Kappa Light Chain ◽  
Light Chain Gene ◽  
Chain Gene Rearrangement ◽  
Cell Acute Lymphoblastic Leukemia

scholarly journals Molecular requirements for the mu-induced light chain gene rearrangement in pre-B cells.

1991 ◽  
Vol 10 (8) ◽  
pp. 2147-2155 ◽  
Author(s):  
A. Iglesias ◽  
M. Kopf ◽  
G.S. Williams ◽  
B. Bühler ◽  
G. Köhler
Keyword(s):  
B Cells ◽  
Light Chain ◽  
Gene Rearrangement ◽  
Chain Gene ◽  
Light Chain Gene ◽  
Chain Gene Rearrangement
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