scholarly journals Evaluation of growth and production of the threatened giant river catfish, Sperata seenghala (Sykes) in polyculture with indigenous major carps

2011 ◽  
Vol 10 (15) ◽  
pp. 2999-3008 ◽  
Author(s):  
Aminur Rahman M ◽  
Arshad A ◽  
Nurul Amin S M
Keyword(s):  
Sperata Seenghala ◽  
Major Carps

Nutrients budget and effluents characteristics in polyculture of scampi (Macrobrachium rosenbergii) and Indian major carps ponds using organic inputs

2012 ◽  
Vol 66 (7) ◽  
pp. 1540-1548 ◽  
Author(s):  
Subhendu Adhikari ◽  
Bharat Chandra Sahu ◽  
Lambodar Dey
Keyword(s):  
Macrobrachium Rosenbergii ◽  
Nutrient Budget ◽  
Catla Catla ◽  
Average Weight ◽  
Cow Dung ◽  
Nutrient Loads ◽  
Feed Conversion ◽  
Indian Major Carps ◽  
Main Input ◽  
Major Carps

Experiments were conducted for the study of nutrient budget in farmers' ponds (0.4–0.6 ha) in Orissa, India, at stocking densities of 0.30–0.38/m2 for scampi (Macrobrachium rosenbergii) and 0.60–0.70/m2 for Indian major carps (Catla catla, Labeo rohita and Cirrhinus mrigala). The average initial body weights of scampi and the major carps were 0.02 and 8–10 g, respectively. The culture period was 272–292 days. Feed was the main input. The FCR (feed conversion ratio) varied from 1.78 to 1.83. Feed and cow dung were applied to these ponds as organic inputs. At harvest time, the average weight of scampi and carps varied from 73 to 92 g and from 718 to 820 g, respectively. Among all the inputs, feed alone accounted for 97.60% total nitrogen (N), 97.90% total phosphorus (P) and 94.72% total organic carbon (OC), respectively. The harvest of scampi and carps accounted for recovery of 52.45% N, 19.43% P and 18.12% OC, respectively. N, P and OC accumulated in sediment were 38.31, 71.40 and 69.50%, respectively. The median nutrient loads in the fish pond effluents were equivalent to 0.58 kg of inorganic N, 0.135 kg of P and 8.83 kg of total OC per ton of Indian major carps and scampi production.

scholarly journals Genetic characterization and phylogenetic study of Indonesian indigenous catfish based on mitochondrial cytochrome B gene

2020 ◽  
Vol 13 (1) ◽  
pp. 96-103
Author(s):  
Dorothea Vera Megarani ◽  
Herjuno Ari Nugroho ◽  
Zahrah Prawita Andarini ◽  
Yura Dwi Risa B. R. Surbakti ◽  
Rini Widayanti
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Cytochrome B ◽  
Genetic Characterization ◽  
Phylogenetic Study ◽  
Cyt B ◽  
Phylogenetic Structure ◽  
Mitochondrial Cytochrome B ◽  
Sperata Seenghala ◽  
Cyt B Gene

Aim: This study aimed to determine the genetic characterization and phylogenetic structure of Indonesian indigenous catfish using cytochrome B (Cyt B) sequences. Materials and Methods: The genomes of 26 catfishes caught from nine rivers from nine different geographical locations around Indonesia were analyzed. The tissue isolation method was used to isolate the total genome of the fishes. Furthermore, polymerase chain reaction was done to amplify the mtDNA Cyt B using the CytBF and CytBR primers. Following sequencing, the analysis of genetic variation and the phylogenetic relationship was performed using MEGA version X software. Results: Cyt B gene sequencing attained a total of 1139 nucleotides encrypting 379 amino acids for all samples. The ClustalW alignment program using MEGA X software revealed 395 substituted nucleotides, which then translated into 63 amino acid variation sites among all 26 samples. No amino acids in catfish BB were different compared to catfish PM, MP, and KR2,3. Catfish MS had one modified amino acid; KR1 and KS had two different amino acids; BF had 38 different amino acids; EM had 31 different amino acids; and BSBJ had 26 different amino acids compared to catfish BB. The most significant alteration of amino acids was between catfish EM and BF (49 amino acids). Conclusion: Indonesian catfish were divided into five clades based on the Cyt B gene. Samples KR and MP (Sumatra); MS and BB (Kalimantan); and PM (Java) were clustered with Hemibagrus nemurus and Hemibagrus wyckioides (Bagridae family). Samples from Kalimantan (KS) and one sample of KR (KR1) from Sumatra were clustered with Sperata seenghala and Hemibagrus spilopterus (Bagridae family). Samples from Java (BSBJ) were clustered with Pseudolais pleurotaenia (Pangasiidae family). Samples EM (Java) were together with Mystus cavasius (Bagridae family). Samples from West Papua were clustered with Potamosilurus latirostris (Ariidae family).

scholarly journals Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822)

2020 ◽  
Vol 67 (2) ◽  
Author(s):  
Snatashree Mohanty ◽  
M. Makesh ◽  
K. V. Rajendran ◽  
P. P. Suresh Babu ◽  
Deepika Anand ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cross Reactivity ◽  
Catla Catla ◽  
Cirrhinus Mrigala ◽  
Indian Major Carps ◽  
Sds Page ◽  
Igg1 Isotype ◽  
Major Carps

Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purified by affinity chromatography using BSA-CL agarose column. The purified mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purified m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purified m-Ig indicated that, all the three MAbs were specific to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps.

scholarly journals Comparison of the Growth Potential of Macrobrachium rosenbergii in Mono and Polyculture Conditions in Earthen Culture Ponds of Jammu, India

2019 ◽  
Vol 19 (3&4) ◽  
pp. 112
Author(s):  
Sanjeev Kumar Karlopia ◽  
Yahya Bakhtiyar ◽  
Seema Langer
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Macrobrachium Rosenbergii ◽  
Growth Potential ◽  
Growth And Survival ◽  
Indian Major Carps ◽  
Freshwater Ponds ◽  
Mean Size ◽  
Average Size ◽  
Major Carps

The study was conducted to assess the growth and survival of <em>Macrobrachium rosenbergii</em> in earthen culture ponds in order to know about the growth potential of <em>M. rosenbergii</em> in mono as well as polyculture conditions in Jammu. Polyculture experiments of prawn with some selected carps Indian Major Carps (<em>Cirrhinus mrigala</em> and <em>Labeo rohita</em>) and Exotic Carps (<em>Cyprinus carpio</em>) were undertaken in 3 freshwater ponds for duration of 4 months. Under the monoculture conditions, prawns attained an average size of 10.40 ± 0.17 cm weighing 23.84 ± 0.32 g in 4 months and the survival rate was found to be more than 75%. The growth rate of prawns was found to increase steadily during the first half (upto mid-October) after which a decline in the growth rate was evident as the mean temperature reached below 18°C. In the polyculture ponds the prawns attained an average size of 10.10 ± 0.03 cm weighing 18.39 ± 0.89g (without<em> C. carpio</em>) and 8.4 ± 0.36 cm weighing 15.23 ± 0.36 g (with <em>C. carpio</em>) in 4 months and their survival rate was less than 70%. Prawns being benthic feeders utilized the leftover food (of the fishes) that settled at the bottom, therefore, saving the feed cost. It was observed that mean size of the fish attained in the polyculture practice was almost similar to that obtained in the monoculture. The ideal prawn polyculture should be practiced with fast growing compatible carps such as Rohu and grass carp. Bottom feeder carps such as Mrigal (<em>C. mrigala</em>) should be avoided in prawn polyculture practice as they compete with prawns in feeding.

scholarly journals The Potential for Homestead Pond Polyculture of Tilapia and Carps in Coastal Bangladesh

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Md. Abdul Wahab ◽  
Md. Jimi Reza ◽  
Mir Mohammad Ali ◽  
Md. Nahiduzzaman
Keyword(s):  
Quality Parameters ◽  
Catla Catla ◽  
Cost Ratio ◽  
Participatory Action ◽  
Coastal Bangladesh ◽  
Benefit Cost Ratio ◽  
Fishing Villages ◽  
Income Opportunity ◽  
Benefit Cost ◽  
Major Carps

A fishers’ women-led Participatory Action Research (PAR) was conducted in 30 homestead ponds to assess the potential for polyculture of Tilapia (Oreochromis niloticus) and major carps Rohu (Labeo rohita) and Catla (Catla catla) in two coastal fishing villages of Bangladesh. Three treatments, namely T1 (Tilapia 200 fish per decimal; 1 decimal=40 m2), T2 (Tilapia 200+ Rohu 32+ Catla 8 fish per decimal) and T3 (Tilapia 200+ Rohu 8+ Catla 32 fish per decimal), each with 5 replicates, were tried in Hossainpur and Anipara villages. Formulated commercial Mega-feed was applied to the ponds twice daily at an initial rate of 10% body weight (bw)/day of Tilapia down to 4% bw/day throughout the culture period. The water quality parameters of ponds including temperature, transparency, pH, salinity and dissolved oxygen significantly varied among treatments except temperature and pH and remained within optimum range for carp polyculture except salinity. The survival rate, harvesting weight and yield of Tilapia were significantly highest in T1 (85.63±05%, 258.59±18.76 g & 11073±805 kg/ha, respectively) in Anipara and lowest in T3 (75.63±0.37%, 136.97±10.63 g & 5180±406 kg/ha, respectively) in Hossainpur. The gross fish production was the significantly highest in T1 (11354±806 kg/ha) of Anipara and lowest in T1 (6325±227 kg/ha) of Hossainpur. Statistically, the highest net return (866,627±84874 BDT/ha) was found in T1 of Anipara and lowest in T3 (279,389±46104 BDT/ha) of Hossainpur with a significantly higher benefit-cost ratio (BCR) obtained in T1 (3.26±0.20) for Tilapia and lower in T3 (1.58±0.10) for polyculture Tilapia and carp of Hossainpur. Therefore, it may be concluded that Tilapia production in small homestead coastal ponds has a higher potential than its mix with carps in polyculture for generating food and supplemental income opportunity for coastal fishers’ women in Bangladesh.

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