The effect of drought stress and exogenous abscisic acid on growth, protein content and antioxidative enzyme activity in saffron (Crocus sativus L.)

Abstract Background: The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses has been studied in plants, however, limited is known about the 4CL genes in cotton (G. hirsutum L.) and their roles in response to drought stress.Results: We performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis-elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 (including Gh4CL7) were up-regulated under the polyethylene glycol (PEG) simulated drought stress conditions. Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7-silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2). Arabidopsis lines overexpressing Gh4CL7, however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H2O2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7-silencing cotton plants and the Gh4CL7-overexpressing Arabidopsis lines had a ~20% reduction and a ~10% increase in lignin content, respectively. The expression levels of genes related to lignin biosynthesis, including PAL, CCoAOMT, COMT, CCR and CAD, were lower in Gh4CL7-silencing plants than in controls. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton.Conclusion: We characterized the 4CL gene family in upland cotton and revealed a role of Gh4CL7 in lignin biosynthesis and drought tolerance.

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Plant Growth, Antioxidative Enzymes and Lipid Peroxidation in Sunflower Seedlings Supplemented With Eichhornia crassipes Organic Fertilizer Under Drought Stress Conditions

Journal of Agricultural Science ◽

10.5539/jas.v11n1p504 ◽

2018 ◽

Vol 11 (1) ◽

pp. 504

Author(s):

Rifandreo Monteiro Barbosa ◽

Paulo Ovídio Batista de Brito ◽

Francisca Raíssa da Silva Costa ◽

Roberto Albuquerque Pontes Filho ◽

Francisco Holanda Nunes Júnior ◽

...

Keyword(s):

Lipid Peroxidation ◽

Enzyme Activity ◽

Drought Stress ◽

Plant Growth ◽

Eichhornia Crassipes ◽

Organic Fertilizer ◽

Antioxidative Enzyme ◽

Stress Conditions ◽

Semiarid Region ◽

Antioxidative Enzyme Activity

In the semiarid region of the Brazilian Northeast, there is still the occurrence of soils with low concentrations of organic mass and nutrients. Eichhornia crassipes (water hyacinth) is recognized as one of the top ten endemic herbs in the world. However, its accumulation capacity means it can be an alternative source of nutrients. The objective of this study was to analyse the effects of macrophyte organic residue (ROM) on plant growth, antioxidative enzyme activity and membrane lipid peroxidation in leaves and roots of sunflower seedlings submitted to drought stress conditions. The experiment was conducted under greenhouse conditions at the Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Maracanaú Campus, Brazil. Samples of E. crassipes were collected in the Parangaba Lagoon, Fortaleza, Ceará, Brazil. The treatments were: 1) 100% (by volume) sand; 2) sand + fertilizer (following the recommendation of 80 kg of N/ha), and 3) sand + 100% of the recommended nitrogen in macrophytes (RN). In general, the use of ROM caused better seedling growth in relation to the other treatments in all conditions studied. Increases in antioxidative enzyme activity and reductions in the deleterious effects of drought stress on plant growth were observed.

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Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

10.21203/rs.2.17480/v2 ◽

2020 ◽

Author(s):

Shichao Sun ◽

Xianpeng Xiong ◽

Xiaoli Zhang ◽

Hongjie Feng ◽

Qianhao Zhu ◽

...

Keyword(s):

Enzyme Activity ◽

Drought Stress ◽

Drought Tolerance ◽

Upland Cotton ◽

Expression Profiles ◽

Evolutionary Relationship ◽

Antioxidative Enzyme ◽

Stress Conditions ◽

Cotton Plants ◽

Antioxidative Enzyme Activity

Abstract Background The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses have been studied in plants, however, limite is known about the upland cotton 4CL genes and their roles in response to drought stress.Results In this study, we performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis -elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 were up-regulated under the PEG simulated drought stress conditions, including Gh4CL7 . Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7 -silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ). Arabidopsis lines overexpressing Gh4CL7 , however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H 2 O 2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7 -silencing cotton plants and the Gh4CL7- overexpressing Arabidopsis lines had a lower and higher lignin content, respectively. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton.Conclusion We comprehensively identified 4CL family genes in upland cotton and provide valuable information on the evolutionary relationship. Gh4CL7 gene is involved in lignin biosynthesis and play a positively role in reponsing drought stress.

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Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

10.21203/rs.2.17480/v1 ◽

2019 ◽

Author(s):

Shichao Sun ◽

Xianpeng Xiong ◽

Xiaoli Zhang ◽

Hongjie Feng ◽

Qianhao Zhu ◽

...

Keyword(s):

Enzyme Activity ◽

Drought Stress ◽

Drought Tolerance ◽

Upland Cotton ◽

Expression Profiles ◽

Evolutionary Relationship ◽

Antioxidative Enzyme ◽

Stress Conditions ◽

Cotton Plants ◽

Antioxidative Enzyme Activity

Abstract Background The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses have been studied in plants, however, limite is known about the upland cotton 4CL genes and their roles in response to drought stress.Results In this study, we performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis -elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 were up-regulated under the PEG simulated drought stress conditions, including Gh4CL7 . Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7 -silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ). Arabidopsis lines overexpressing Gh4CL7 , however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H 2 O 2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7 -silencing cotton plants and the Gh4CL7- overexpressing Arabidopsis lines had a lower and higher lignin content, respectively. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton.Conclusion We comprehensively identified 4CL family genes in upland cotton and provide valuable information on the evolutionary relationship. Gh4CL7 gene is involved in lignin biosynthesis and play a positively role in reponsing drought stress.

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