Genetic diversity in green gram accessions as revealed by STMS markers

2011 ◽  
Vol 10 (75) ◽  
Author(s):  
Avinash Pandey
Sugar Tech ◽  
2005 ◽  
Vol 7 (2-3) ◽  
pp. 9-14 ◽  
Author(s):  
G. Hemaprabha ◽  
P. Govindaraj ◽  
N. Balasundaram ◽  
N. K. Singh

2006 ◽  
Vol 107 (4) ◽  
pp. 352-357 ◽  
Author(s):  
T.K. Behera ◽  
Pankaj Sharma ◽  
B.K. Singh ◽  
Gunjeet Kumar ◽  
Ravinder Kumar ◽  
...  

Author(s):  
Reecha. T. Das ◽  
Purna K. Barua

Twenty three advanced breeding lines of green gram, developed at different centres under the All-India Coordinated Research Project on MULLARP, were evaluated by growing them during summer to characterize the genotypes and to assess the magnitude of genetic diversity in the genotypes. Genetic dissimilarities as revealed by Euclidean distances ranged from 2.891 to 10.494. Maximum distance was observed between ML 1354 and MH 721. Based on Euclidean distances four clusters were formed. Cluster II was the largest, and was further divided into two sub-clusters, IIA and IIB, comprising of four and nine genotypes, respectively. Genotypes MH 709 and KM 2272 belonging to cluster I were closely placed as they shared most of the characters. But they could be distinguished with the help of characters like days to 50% flowering, position of pods in the plant and growth habit. Similarly, GM 04-02 and NM 1 in sub-cluster IIA were closely grouped, and they could be distinguished based on the characters leaf size and pod length. Similarly other closely grouped genotypes were also distinguishable on the basis of some characters. It was observed from the average Euclidean distances that ML 1354, SG 1, MH 721, SG 63-14 and MH 709 were divergent genotypes. These genotypes were potential parents in green gram breeding programme.


Author(s):  
Syeda Asma Koinain ◽  
V S Hegde ◽  
C . Bharadwaj

Genetic diversity among 30 chickpea genotypes was evaluated using simple sequence repeat (SSR) molecular markers. The studies using Sequence Tagged Microsatellite Site (STMS) markers markers revealed that among the primers used across the genotypes produced a total of 35 alleles representing 21 SSR loci with frequencies ranging from one to two (mean 1.66) alleles per locus. Polymorphic Information Content (PIC) ranged from 0.098 to 0.500 (CAM0443, CAM0446). These primers might be an effective and useful tool to determine the genetic differences among chickpea genotypes and to study the phylogenetic relationships. Polymorphic percentage was 96.42. Hierarchical neighbour-joining UPGMA cluster analysis based on simple matching similarity matrix resolved the 30 genotypes into seven clusters. Based on STMS markers highest similarity index 0.850 was observed between BGD 72 and Annigeri-1whereas BGD 9920 and ICC 92944 showed the lowest similarity index 0.214 between them. The STMS clustering pattern indicated the presence of wide genetic diversity between the genotypes. Overall, the study ascertained that SSRs provide powerful marker tools in revealing genetic diversity and relationships in chickpeas, thereby proving useful for selection of parents in breeding programs and also for DNA fingerprinting for identification of cultivars.


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