scholarly journals Effect of Streptococcus uberis infections on cell population of bovine mammary gland

2012 ◽  
Vol 6 (7) ◽  
pp. 1359-1363
Author(s):  
Slama Petr ◽  
Havlicek Zdenek ◽  
Skladanka Jiri ◽  
Marada Petr
Keyword(s):  
Mammary Gland ◽  
Cell Population ◽  
Bovine Mammary Gland ◽  
Streptococcus Uberis

Histochemical localization and possible antibacterial role of xanthine oxidase in the bovine mammary gland

1988 ◽  
Vol 55 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Robert A. Collins ◽  
Keith R. Parsons ◽  
Terry R. Field ◽  
A. John Bramley
Keyword(s):  
Antibacterial Activity ◽  
Mammary Gland ◽  
Xanthine Oxidase ◽  
Histochemical Localization ◽  
Bovine Mammary Gland ◽  
Streptococcus Uberis ◽  
Antibacterial Assay ◽  
Secretory Tissue ◽  
Teat Canal

SummaryXanthine oxidase (XO) was demonstrated to be present in the teat canal and secretory tissue of the bovine mammary gland by histochemical techniques. Homogenates of these tissues were able to replace XO in an antibacterial assay with Streptococcus uberis. The action of XO on its substrate hypoxanthine was shown to provide an essential component for anti-streptococcal activity mediated by lactoperoxidase. A mechanism is proposed whereby the interaction of XO, lactoperoxidase and thiocyanate may provide antibacterial activity in the teat canal.

scholarly journals MtuA, a Lipoprotein Receptor Antigen from Streptococcus uberis, Is Responsible for Acquisition of Manganese during Growth in Milk and Is Essential for Infection of the Lactating Bovine Mammary Gland

2003 ◽  
Vol 71 (9) ◽  
pp. 4842-4849 ◽  
Author(s):  
Amanda J. Smith ◽  
Philip N. Ward ◽  
Terence R. Field ◽  
Catherine L. Jones ◽  
Ruth A. Lincoln ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Inflammatory Response ◽  
Metal Binding ◽  
Parental Strain ◽  
Insertional Mutagenesis ◽  
Bovine Milk ◽  
Bovine Mammary Gland ◽  
Streptococcus Uberis ◽  
Efficient Uptake ◽  
Single Insertion

ABSTRACT A mutant strain of Streptococcus uberis (AJS001) that was unable to grow in bovine milk was isolated following random insertional mutagenesis. The level of growth in milk was restored to that of the parental strain (strain 0140J) following addition of MnSO4 but not following addition of other metal ions. The mutant contained a single insertion within mtuA, a homologue of mtsA and psaA, which encode metal-binding proteins in Streptococcus pyogenes and Streptococcus pneumoniae, respectively. Strain AJS001 was unable to infect any of eight quarters on four dairy cows following intramammary challenge with 105 CFU. Bacteria were never recovered directly from milk of these animals but were detected following enrichment in Todd-Hewitt broth in three of eight milk samples obtained within 24 h of challenge. The animals showed no inflammatory response and no signs of mastitis. Three mammary quarters on two different animals simultaneously challenged with 600 CFU of the parental strain, strain 0140J, became colonized, shed high numbers of S. uberis organisms in milk, displayed a marked inflammatory response to infection, and showed overt signs of mastitis. These data indicate that mtuA was required for efficient uptake of Mn2+ during growth in bovine milk and infection of the lactating bovine mammary gland.

scholarly journals Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland neutrophils in vitro

2012 ◽  
Vol 50 (No. 1) ◽  
pp. 11-23 ◽  
Author(s):  
Z. Sladek ◽  
D. Rysanek ◽  
M. Faldyna
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Death ◽  
Mammary Gland ◽  
Programmed Cell Death ◽  
Propidium Iodide ◽  
Annexin V ◽  
Dna Fragments ◽  
Bovine Mammary Gland ◽  
Streptococcus Uberis

Neutrophils play an important role in the defence of the bovine mammary gland against bacterial infections. In the course of the resolution of mammary gland inflammation, neutrophils undergo programmed cell death – apoptosis. The aim of this study was to confirm whether the co-cultivation of neutrophils of the bovine mammary gland with either Staphylococcus aureus or Streptococcus uberis leads to signs of apoptosis. In the study, 16 mammary glands of four virgin heifers aged 16 to 18 months were examined. Neutrophils were obtained by lavage after an induced influx. After a three-hour incubation of the neutrophils with bacteria in vitro, neutrophil apoptosis was detected by morphological features, by determination of histone-associated DNA fragments (ELISA), and by Annexin -V and propidium iodide positivity (flow cytometry). S. aureus and S. uberis reduced the incidence of karyopycnotic and zeiotic neutrophils (P < 0.01), and insignificantly reduced the concentration of histone -associated DNA fragments (P > 0.05). The incubation of neutrophils with bacteria, however, increased the proportion of Annexin –V-positive cells (P < 0.01) and Annexin -V and propidium iodide-positive cells (P < 0.05). Co-cultivation of neutrophils with either S. aureus or S. uberis led to the induction of phosphatidylserine translocation characteristic of the early stage of apoptosis. The late signs of apoptosis were delayed by co-cultivation of neutrophils with both pathogens. Therefore it is obvious that although the programmed cell death of apoptosis is initiated by these pathogens, the completion of the program is delayed.

scholarly journals Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3594
Author(s):  
Petr Slama ◽  
Terezie Zavadilova ◽  
Ales Pavlik ◽  
Pavel Horky ◽  
Sylvie Skalickova ◽  
...  
Keyword(s):  
T Cells ◽  
Mammary Gland ◽  
Gland Cell ◽  
Γδ T Cells ◽  
Mammary Glands ◽  
Cell Phenotype ◽  
Bovine Mammary Gland ◽  
Gamma Delta ◽  
Streptococcus Uberis ◽  
Gamma Delta T Cell

In this study, we focused analyzing γδ T cells during bovine mammary gland inflammation induced by Streptococcus uberis. A mammary gland cell suspension was obtained using lavage 24, 48, 72, and 168 h after intramammary-induced infection. The proportion of lymphocytes increased during the entire week in which inflammation was present. The γδ T cells were also elevated during inflammation, reaching their peak at 72 h following induced inflammation. The percentage of apoptotic lymphocytes continually increased, with the highest proportion occurring 168 h after S. uberis infection. The results show that γδ T cells may be involved in the resolution of inflammation in bovine mammary glands, with the apoptosis of those cells potentially playing an important role.

scholarly journals The Activation of Bovine Plasminogen by PauA Is Not Required for Virulence of Streptococcus uberis

2003 ◽  
Vol 71 (12) ◽  
pp. 7193-7196 ◽  
Author(s):  
Philip N. Ward ◽  
Terence R. Field ◽  
Christopher D. Rapier ◽  
James A. Leigh
Keyword(s):  
Mammary Gland ◽  
Single Copy ◽  
Bovine Mammary Gland ◽  
Streptococcus Uberis

ABSTRACT A mutant of Streptococcus uberis carrying a single copy of ISS1 within pauA was unable to activate bovine plasminogen. Contrary to a hypothesis postulated previously, this mutation did not alter the ability of the bacterium to grow in milk or to infect the lactating bovine mammary gland.

Ultrastructural Changes in the Mammary Epithelial Cell Population During Neoplastic Development Induced by A Chemical Carcinogen.

1976 ◽  
Vol 34 ◽  
pp. 250-251 ◽  
Author(s):  
J. Russo ◽  
W. Isenberg ◽  
M. Ireland ◽  
I.H. Russo
Keyword(s):  
Mammary Gland ◽  
Cell Population ◽  
Virgin Female ◽  
Histological Change ◽  
Mammary Epithelial ◽  
Female Rats ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Chemical Carcinogen ◽  
Sprague Dawley

The induction of rat mammary carcinoma by the chemical carcinogen DMBA is used as a model for the study of the human disease (1). We previously described the histochemical changes that occur in the mammary gland of DMBA treated animals before the earliest manifested histological change, the intraductal proliferation (IDP), was observed (2). In the present work, we demonstrate that a change in the stable cell population found in the resting mammary gland occurs after carcinogen administration.Fifty-five day old Sprague-Dawley virgin female rats were inoculated intragastrically with 20mg of 7,12-dimethylbenz(a)anthracene (DMBA) in 1ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from control and inoculated rats were removed weekly from the time of inoculation until 60 days post-inoculation. For electron microscopy, the glands were immersed in Karnovsky's fixative, post-fixed in 1% OsO4, dehydrated, and embedded in an Epon-Araldite mixture. Thick (lμ) sections were stained with 1% toluidine blue and were used for selecting areas for ultrastructural study.

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