Evaluation of specimens in which the urine sediment analysis was conducted by full-automatic systems and a manual method together with urine culture results

2011 ◽  
Vol 5 (15) ◽  
Author(s):  
Okhan Kadir AKIN
Keyword(s):  
Urine Culture ◽  
Urine Sediment ◽  
Manual Method ◽  
Sediment Analysis ◽  
Automatic Systems

A New Screening Approach For Fast And Accurate Prediction Of Positive And Negative Urine Cultures By SediMAX Compared With The Standard Urine Culture

2021 ◽  
Vol 1 (1) ◽  
pp. 46-55
Author(s):  
Massimo Pieri ◽  
Flaminia Tomassetti ◽  
Paola Cerini ◽  
Roberta Felicetti ◽  
Lucia Ceccaroni ◽  
...  
Keyword(s):  
Blood Cell ◽  
Bacterial Infections ◽  
Urine Culture ◽  
Screening Method ◽  
Significant Proportion ◽  
Bacterial Count ◽  
Urine Samples ◽  
Screening Methods ◽  
Urine Sediment ◽  
Tract Infections

Urinary tract infections (UTI) are the most frequent bacterial infections, and the detection of infection in urine samples is expensive and time-consuming. Also, in laboratories a significant proportion of samples processed yield negative results. For this, screening methods represent an important improvement towards the final UTI diagnosis. SediMAX is an automated microscopy, easier to use in laboratories due to its basic procedure and it is widely used for urine sediment analysis. In our study, we evaluated the performance of SediMAX, applying some screening parameters, compared with the gold standard methods, urine culture, to identify all the positive cases for UTI. We analysed 1185 urine samples from our daily laboratory routine. The basis of our screening model was to establish a cut-off for bacterial count (BACT), as 300 bacteria/µL in order to avoid missing positive cases. However, the sensitivity and the specificity achieved were not enough to identify all UTI infection in urine samples. So, in addition to BACT we have considered other parameters, such as White Blood Cell (WBC), Red Blood Cell (RBC), Yeasts (YEST), Age and Nitrates (NIT). The second screening method reached a sensitivity of 100%, that could be reliably employed in detect of UTIs.

An approach to standardization of urine sediment analysis via suggestion of a common manual protocol

2016 ◽  
Vol 76 (3) ◽  
pp. 256-263 ◽  
Author(s):  
Dae-Hyun Ko ◽  
Misuk JI ◽  
Sollip Kim ◽  
Eun-Jung Cho ◽  
Woochang Lee ◽  
...  
Keyword(s):  
Urine Sediment ◽  
Sediment Analysis

scholarly journals Asymptomatic bacteriuria in pregnancy: what is the validity of urine sediment microscopy as a screening tool in a low resource setting?

2020 ◽  
Vol 9 (6) ◽  
pp. 2442
Author(s):  
Rashmi Polnaya ◽  
Amritha Bhandary
Keyword(s):  
Predictive Value ◽  
Urine Culture ◽  
Asymptomatic Bacteriuria ◽  
Urine Sediment ◽  
Predictive Values ◽  
Low Resource ◽  
Resource Setting ◽  
Low Resource Setting ◽  
Urine Microscopy ◽  
In Pregnancy

Background: Asymptomatic bacteriuria in pregnancy should be screened and treated to reduce the risk of morbidity and mortality. Urine culture is the recommended test. In low resource setting with large patient load urine culture in all pregnant patients is not feasible. In this study authors have assessed the validity of urine microscopy in the screening of asymptomatic bacteriuria in pregnancy.Methods: Midstream clean catch urine specimen collected from 675 pregnant women was subjected to urine sediment microscopy and culture. It was considered screen positive if pus cells were >5/HPF. Asymptomatic bacteriuria was diagnosed if there were >/=100000 CFU of a single uropathogen per ml. Results obtained were statistically analysed for the prevalence of asymptomatic bacteriuria, sociodemographic and medical risk factors, causative organisms, antibiotic sensitivity and validity of urine sediment microscopy in detecting asymptomatic bacteriuria.Results: The incidence of asymptomatic bacteriuria in our study was 10.2%. The incidence was higher in the age group between 20-30 years, in gravida 3 and above, in upper lower and lower socioeconomic status, in women with hyperglycemia in pregnancy and anaemia. E. coli was the commonest bacterial isolate in culture positive cases. Bacterial isolates had poor sensitivity for Ampicillin, amoxicillin and nitrofurantoin and good sensitivity for cephalosporins and aminoglycosides. The sensitivity, specificity, positive and negative predictive values for urine microscopy was 43%, 85%, 25% and 93% respectively.Conclusions: This study shows poor sensitivity and positive predictive value and good specificity and negative predictive value.

Review of Enterococci Isolated from Canine and Feline Urine Specimens from 2006 to 2011

2015 ◽  
Vol 51 (3) ◽  
pp. 148-154 ◽  
Author(s):  
Kate S. KuKanich ◽  
Brian V. Lubbers
Keyword(s):  
Medical Records ◽  
Urine Culture ◽  
Bacterial Species ◽  
Clinical Signs ◽  
Multidrug Resistant ◽  
Urine Sediment ◽  
Colony Forming Units ◽  
Enterococcus Spp ◽  
Tract Infections ◽  
Urine Specimens

Canine and feline urine culture reports and medical records were reviewed at a veterinary teaching hospital from 2006 to 2011 for enterococcal growth, coinfections, antimicrobial resistance, urine sediment findings, clinical signs, and concurrent conditions. Of all of the urine specimens with significantly defined colony-forming units/mL, Enterococcus (E.) faecalis was the only enterococci isolated from cats and predominated (77.4%) in dogs followed by E. faecium (12.9%), E. durans (3.2%), and other Enterococcus spp. (6.5%). The majority of specimens with significant enterococcal growth resulted in complicated urinary tract infections in 83.9% of dogs and 81.8% of cats. Specimens with only enterococcal growth were more common than those mixed with other bacterial species. Cocci were observed in urine sediments of 8 out of 8 cats and 21 out of 25 dogs with available concurrent urinalyses. Pyuria was noted in 5 out of 8 feline and 15 out of 25 canine urine sediments, and pyuria in dogs was associated with growth of only enterococci on aerobic urine culture. Multidrug resistance was identified in 6 out of 11 cats and 7 out of 31 dogs, and E. faecium isolates from dogs were 4.5× more likely to be multidrug resistant than E. faecalis.

Urine sediment analysis: Analytical and diagnostic performance of sediMAX® — A new automated microscopy image-based urine sediment analyser

2010 ◽  
Vol 411 (3-4) ◽  
pp. 147-154 ◽  
Author(s):  
Zahur Zaman ◽  
Giovanni Battista Fogazzi ◽  
Giuseppe Garigali ◽  
Maria Daniela Croci ◽  
Gabor Bayer ◽  
...  
Keyword(s):  
Diagnostic Performance ◽  
Urine Sediment ◽  
Sediment Analysis ◽  
Microscopy Image ◽  
Automated Microscopy

scholarly journals The role of organic urine sediment analysis in the monitoring of renal diseases

2019 ◽  
Vol 4 (1) ◽  
pp. 40-50
Author(s):  
Muhamed Katica ◽  
Nasreldin Hassan Ahmed ◽  
Alen Salkić ◽  
Adis Mukača ◽  
Ajdin Bašić ◽  
...  
Keyword(s):  
Renal Diseases ◽  
Urine Sediment ◽  
Sediment Analysis

scholarly journals Urate-Lowering Agents in Asymptomatic Hyperuricemia: Role of Urine Sediment Analysis and Musculoskeletal Ultrasound

2018 ◽  
Vol 43 (2) ◽  
pp. 606-615 ◽  
Author(s):  
Davide Viggiano ◽  
Giuseppe Gigliotti ◽  
Gianfranco Vallone ◽  
Anna Giammarino ◽  
Michelangelo Nigro ◽  
...  
Keyword(s):  
Musculoskeletal Ultrasound ◽  
Urine Sediment ◽  
Sediment Analysis

scholarly journals The prevalence of positive urine cultures in 100 dogs with an inactive urine sediment

2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Rachel Liebelt ◽  
Armi Pigott
Keyword(s):  
Urinary Tract ◽  
Private Practice ◽  
Lower Urinary Tract ◽  
Urine Culture ◽  
Retrospective Chart Review ◽  
Urine Samples ◽  
Current Guideline ◽  
Urine Sediment ◽  
Referral Hospitals ◽  
Lower Urinary

Objective: The purpose of this study was to determine the prevalence of positive quantitative urine cultures in dogs with an inactive urine sediment. Background: A urinalysis is a useful screening tool for the evaluation of evidence of bacterial cystitis and a quantitative urine culture is used for definitive diagnosis. Evidentiary value: A retrospective chart review from June 2012 to December 2017 at three private practice emergency and specialty referral hospitals examined urine samples obtained from 100 client-owned dogs. Methods: The signalment and clinicopathologic data was recorded for all canine patients that had urine samples obtained by cystocentesis that had an inactive sediment exam on urinalysis and subsequent quantitative urine cultures were performed. Results: The prevalence of positive quantitative urine cultures in all dogs with an inactive urine sediment at the aforementioned institutions was 6% (6/100). Escherichia coli was the most commonly isolated bacteria. Conclusion: Given the low prevalence of positive quantitative urine cultures in dogs with an inactive urine sediment and current guideline recommendations for management of subclinical bacteriuria, we do not recommend urine cultures for dogs without lower urinary tract signs. Further prospective study of patient subgroups, as well as controlled studies evaluating urine sample handling techniques using methods available to private practice practitioners are sorely needed. Application: Emergency and general practicing veterinarians should consider a quantitative urine culture for dogs with lower urinary tract signs, even with an inactive sediment examination, and on a case-by-case basis for dogs with pertinent systemic diseases or known risk factors for bacterial cystitis.  

scholarly journals Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats

2021 ◽  
pp. 104063872110389
Author(s):  
Elisabeth Neubert ◽  
Karin Weber
Keyword(s):  
Bacterial Growth ◽  
High Power ◽  
Leukocyte Count ◽  
Urine Samples ◽  
High Power Field ◽  
Urine Sediment ◽  
Sediment Analysis ◽  
Accurate Detection ◽  
Culture Positive ◽  
Culture Negative

We analyzed urine samples from 191 cats for bacteriuria with an automated urine sediment analyzer (Idexx SediVue Dx), combined with image review by an observer, and compared to bacteriologic culture results. Sixty-nine samples were unambiguously assigned to be free of bacteria by the instrument and the observer, and no bacterial growth was detected. Twenty-seven samples were unambiguously assigned to have bacteriuria; 24 of these 27 samples were culture-positive. For these samples, bacteriuria was predicted with a sensitivity of 100% and a specificity of 96%. A clear assignment was not possible for 95 samples, 81 of which were culture-negative. Specificity dropped to 45% when all samples were considered. Using the automated leukocyte count to predict bacteriuria, sensitivity was 82% and specificity was 75%. Automated sediment analysis is faster and less observer-dependent than sediment analysis under a microscope, but accurate detection of bacteriuria remains difficult in a large proportion of samples. Bacteriuria was significantly associated with leukocyte count; the leukocyte count was >5/high power field in 82% of culture-positive samples.

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