scholarly journals Phytochemical analysis and investigation of sedative-hypnotic activity of methanolic stem bark extract of Ficus abutilifolia in mice

2019 ◽  
Vol 13 (13) ◽  
pp. 156-162
Author(s):  
H. G. Mikail ◽  
D. D. Akumka ◽  
M. Adamu ◽  
A. U. Zaifada
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Stem Bark Extract ◽  
Hypnotic Activity

scholarly journals The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Leslie B. Essel ◽  
David D. Obiri ◽  
Newman Osafo ◽  
Aaron O. Antwi ◽  
Babatunde M. Duduyemi
Keyword(s):  
Acute Toxicity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Tissue Level ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Murine Models ◽  
Paw Oedema ◽  
Stem Bark Extract ◽  
Anti Inflammatory

We investigated the antioxidant and anti-inflammatory effects of a 70% v/v ethanol extract of the stem bark of Antrocaryon micraster on murine models of carrageenan-induced pleurisy and paw oedema. Rat pleural fluid was analysed for volume, protein content, and leucocytes, while lung histology was assessed for damage. Lung tissue homogenates were assayed for glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO). Phytochemical analysis was carried out on the stem bark. Acute toxicity studies were conducted in rats. In the pleurisy model the extract (30–300 mg/kg) significantly reduced the volume and amount of proteins and leucocytes in the exudate and also protected against lung injury. Tissue level of GSH and SOD and CAT expression were increased while MDA level and MPO activity were reduced. The peak and total oedema responses were significantly suppressed when given both preemptively and curatively in the mice paw oedema test. Saponins, alkaloids, triterpenoids, and tannins were present in the stem bark. A. micraster extract exhibited no apparent acute toxicity. We conclude that the ethanolic stem-bark extract of A. micraster has antioxidant action and exhibits significant anti-inflammatory activity through suppression of pleurisy and paw oedema induced with carrageenan.

scholarly journals In Vivo Antiplasmodial Activity of Detarium microcarpum (Fabaceae) Stem Bark Extract

Thrita ◽  
2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Amira Rahana Abdullahi ◽  
Sani Malami ◽  
Lawal Alhassan Bichi
Keyword(s):  
Hematological Parameters ◽  
Lethal Dose ◽  
Parasitic Infection ◽  
Stem Bark ◽  
Antiplasmodial Activity ◽  
Bark Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Detarium Microcarpum ◽  
Stem Bark Extract

Background: Malaria is a parasitic infection that is common in the tropical and sub-tropical regions of the world. It is estimated to kill one million individuals annually. Detarium microcarpum (Fabaceae family) is used traditionally for the treatment of malaria, diabetes, hypertension, convulsions, pneumonia, leprosy, and meningitis. The purpose of this research was to evaluate the antiplasmodial potential and safety profile of the methanol extract of D. microcarpum stem bark. Methods: The extract was subjected to phytochemical screening and oral median lethal dose (LD50) estimation. Using curative, suppressive, and prophylactic experimental animal models, antiplasmodial activity was assessed in mice infected with chloroquine-sensitive Plasmodium berghei berghei. Biochemical and hematological tests were conducted in rats orally administered with the extract for 28 days. One-way analysis of variance (ANOVA) was used to analyze the data, followed by Dunnett's post hoc test. Results: Alkaloids, flavonoids, saponins, tannins, triterpenes, and glycosides were found in the extract through phytochemical analysis. When compared to the negative control group, the extract at the tested doses (200, 400, and 800 mg/kg) showed a significant (P < 0.001) curative, suppressive, and prophylactic effect and significantly (P < 0.001) prolonged the survival time of mice in the treated groups. The extract had an insignificant (P > 0.05) effect on the biochemical and hematological parameters tested. Conclusions: The results suggest that the stem bark extract of D. microcarpum at the doses tested possesses antiplasmodial activity and is relatively safe after short-term use.

scholarly journals Phytochemical Analysis and Antioxidants effects of Methanol Stem Bark Extract of Eucalyptus Camaldulensis in Wister Albino Rats

2021 ◽  
pp. 32-39
Author(s):  
Jude Nwaogu ◽  
Babangida Abubakar ◽  
Mustapha Sahabi Aliyu
Keyword(s):  
Oxidative Stress ◽  
Eucalyptus Camaldulensis ◽  
Stem Bark ◽  
Bark Extract ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Enzymatic Antioxidants ◽  
Significant Difference ◽  
Stem Bark Extract

Aim: The aim is to evaluate the antioxidant potentials of Eucalyptus camaldulensis methanol stem bark extract (ECMSBE) on Wister albino rats. Methodology: The phytochemical screening of ECMSBE was conducted using standard methods. A total of 36 albino rats were used for the antioxidant studies. The rats were divided into six (6) groups of six rats. Control group received distilled water orally at 2ml/kg. Oxidative stress was induced in groups 2 to group 6 CCl4 (1ml/kg, s.c) at every 72hrs for 10 days. Group 2 was untreated while groups 3 – 6 received doses of 50, 100, 150 and 200mg/kg of ECMSBE respectively. On the 11th day, the rats were sacrificed and the liver was removed and homogenised and oxidative stress parameters were determined. Results: Phytochemical analysis of ECMSBE revealed the presence of saponins, flavonoids, tannins, phenols, glycosides, steroids, terpenoids and resins. There was no significant difference (P<.05) between the CCl4 induced group and the group treated with ECMSBE (50mg/kg). However, their concentrations were significantly different from the group treated with ECMSBE (100mg/kg – 200mg/kg) when compared to the group treated with CCl4 Only. The CCl4-induced group had its vitamin A, vitamin C and vitamin E concentrations significant different (P<.05) from the groups treated with ECMSBE (100, 150 and 200mg/kg body weight). There was no significant difference (P>.05) in the levels of SOD, CAT and GPx between the group induced with CCl4 only and the group treated with ECMSBE (50mg/kg), however, it these concentrations were significantly higher (P<.05). The enzymatic antioxidants concentration in the normal control group was not significantly different (P>.05) when compared the group that was treated with ECMSBE (200mg/kg) Conclusion: The result suggest that the extract of E. camaldulensis possessed antioxidant properties which can be used as effective protecting agents against oxidative stress and other diseases.

scholarly journals Effects of Aqueous Stem Bark Extract of Stereospermum kunthianum on Carbon Tetrachloride - Induced Hepatotoxicity in Rats

2021 ◽  
pp. 44-52
Author(s):  
Mahmoud Suleiman Jada ◽  
Mohammed Aliyu Sulaiman ◽  
Muktar Abdulmalik
Keyword(s):  
Antioxidant Activity ◽  
Intraperitoneal Administration ◽  
Stem Bark ◽  
Bark Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Antioxidant Power ◽  
Stem Bark Extract ◽  
Control Group Group

Aim: This study is aimed at evaluating the effects of the aqueous stem bark extract of Stereospermum kunthianum on CCl4-induced hepatotoxicity in rats. Methodology: Analysis of qualitative phytochemical components and antioxidant activity were carried out. Experimental rats were randomly divided into six groups of five rats each. Group 1: served as the normal control group. Group 2: was administered with CCl4 only at a dose of 3 ml/kg b.wt by single intraperitoneal administration. Group 3: served as the standard control group.   Group 4: was administered with 200 mg/kg b.wt of the aqueous stem bark extract + CCl4. Group 5: was administered with 400 mg/kg b.wt of the aqueous stem bark extract + CCl4. Group 6: was administered with 600 mg/kg b.wt of the aqueous stem bark extract+ CCl4. Results: The phytochemical analysis showed the presence of flavonoid, phenols, saponins, and terpenoid while tannins and alkaloids were absent. The antioxidant activity showed that the extract significantly (P<0.05) inhibits Ferric Reducing Antioxidant Power (FRAP) and Thiobarbituric Acid Reactive Substances (TBARS), giving high activity as the concentration of the extract increases. The elevated levels of ALT and AST coupled with Conjugated bilirubin, Total bilirubin, and total protein caused by CCl4 administration were all reduced significantly (P<0.05) by the extract in dose dependent manner. Conclusion: These findings demonstrated that stem bark extract of Stereospernmum kunthianum could be an alternative medication for liver injury.

scholarly journals Antiinflammatory, analgesic and antipyretic effects of dichloromethane stem bark extract of Acacia mellifera

2017 ◽  
Vol 6 (4) ◽  
pp. 239-246
Author(s):  
Sindani Akumu Veronica ◽  
◽  
Koech Samson Cheruiyot ◽  
Maoga Jane Bosibori ◽  
Ireri Moses Munene ◽  
...  
Keyword(s):  
Diclofenac Sodium ◽  
Stem Bark ◽  
Herbal Extract ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Paw Edema ◽  
Stem Bark Extract ◽  
Acacia Mellifera ◽  
Inflammatory Effect

The present study was designed to assess the in vivo antiinflammatory, analgesic and antipyretic effect of the dichloromethane stem bark extract of Acacia mellifera on experimental animal model at three different dose levels of 50, 100 and 150mg/kg body weight respectively. In addition, phytochemical screening of the extract was done. The inflammatory effect was evaluated by use of carragenaan-induced paw edema in mice, pain was determined using formalin-induced writhing in mice and pyrexia was determined by turpentine-induced pyrexia in rats. The activity of the herbal extract was compared to diclofenac sodium and aspirin. The phytochemical analysis showed the presence of alkaloids, cardiac glycosides, flavonoid, saponins, steroids, terpenoids, tannins and phenolics. The plant extract produced marked anti-inflammatory effect in carrageenaninduced mice paw edema assay, suppressed licking response of animals in both phases of the formalin- induced test and exhibited appreciable antipyretic effects in turpentine-induced pyrexia in rats. The study thus supports the folklore use of the plant in the treatment and management of inflammation, pain and pyrexia.

scholarly journals Studies on the Antiulcer Activity of the Chloroform Fraction of Balanites aegyptiaca Stem Bark Extract

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Chinenye J. Ugwah-Oguejiofor ◽  
Oguejiofor M. Ugwah ◽  
Emmanuel U. Etuk ◽  
Shuaibu O. Bello ◽  
Adamu A. Aliero
Keyword(s):  
Stem Bark ◽  
Tween 20 ◽  
Bark Extract ◽  
Antiulcer Activity ◽  
Phytochemical Analysis ◽  
Chloroform Fraction ◽  
Balanites Aegyptiaca ◽  
Standard Drug ◽  
Stem Bark Extract ◽  
Group 2

Background: Balanites aegyptiaca L. Delile (Zygophyllaceae) is a perennial tree found mostly in the desert environments. Decoctions of its stem barks are used traditionally in northern Nigeria to treat wounds, ulcers and stomach ailments. Other ethnomedicinal uses include: treatment of malaria, pain and fever. The aim of this study was to evaluate the antiulcer activity of the chloroform stem bark extract of Balanites aegyptiaca in Wistar rats. Materials and methods: Acute toxicity study on the chloroform fraction of the stem bark extract of Balanites aegyptiaca was carried out using the OECD 425 guidelines at a limit dose of 3000 mg/kg. Phytochemical analysis of the fraction was carried out to detect the presence of alkaloids, tannins, flavonoids, steroids, carbohydrates, saponins and glycosides using standard procedures. The antiulcer activity of the fraction was evaluated in ethanol and indomethacin-induced ulcer models in rats. Rats were allotted into five groups (n=5). Group 1 received 10% Tween 20 (1 ml/kg), group 2 received standard drug, omeprazole (20 mg/kg), and groups 3-5 were the extract groups and received 125, 250 and 500 mg/kg, p.o. respectively of the chloroform fraction of Balanites aegyptiaca. The mean ulcer indices were assessed and the percentage ulcer inhibition calculated. Results: At the limit dose of 3000 mg/kg the fraction of Balanites aegyptiaca did not produce any mortality or observable signs of toxicity in the rats. The phytochemical analysis showed the presence of flavonoids, saponins and steroids. The fraction at the doses of 250 and 500 mg/kg caused a significant (p<0.05) reduction in mean ulcer indices in the ethanol and indomethacin-induced ulcer models when compared to the control. Conclusion: Our study showed that the chloroform fraction of the stem bark extract of Balanites aegyptiaca possesses antiulcer activities. Further studies are needed to isolate the active constituents.

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