Polymerase chain reaction based detection of banana bunchy top virus using coat protein based primers

2014 ◽  
Vol 7 (4) ◽  
pp. 765 ◽  
Author(s):  
R. K. Ponsubrya ◽  
Swapna Alex ◽  
K. Rajmohan ◽  
K. B. Soni
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Chain Reaction ◽  
Banana Bunchy Top Virus ◽  
Polymerase Chain

scholarly journals Reverse-Transcription Polymerase Chain Reaction Detection of Citrus Tristeza Virus in Aphids

Plant Disease ◽  
1997 ◽  
Vol 81 (9) ◽  
pp. 1066-1069 ◽  
Author(s):  
Prem Mehta ◽  
R. H. Brlansky ◽  
S. Gowda ◽  
R. K. Yokomi
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Citrus Tristeza Virus ◽  
Protein Gene ◽  
Aphid Species ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tristeza Virus

A rapid and simple reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection of citrus tristeza virus (CTV) in three aphid species. Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These included the most efficient CTV vector, the brown citrus aphid, Toxoptera citricida; the melon aphid, Aphis gossypii; and the green peach aphid, Myzus persicae, a non-vector for CTV. A short procedure for nucleic acid extraction from single or groups of aphids was developed. Nucleic acid extracts from 1, 3, 5, and 10 aphids with acquisition-access periods of 24 and 48 h were reverse transcribed and amplified using primers for the coat protein gene of the Florida B3 (T-36) isolate of CTV. PCR-amplified fragments of approximately 670 bp were obtained from all the isolates tested and the amplified product from the aphids fed on citrus infected with isolate B3 was confirmed as the CTV coat protein gene by digesting with various restriction enzymes. This technique will be useful in investigations of CTV-vector-plant interactions and CTV epidemiology.

scholarly journals PENGGUNAAN PELACAK NONRADIOAKTIF (Digoxigenin-DNA Probe) UNTUK MENDETEKSI PEANUT STRIPE VIRUS

2001 ◽  
Vol 1 (2) ◽  
pp. 75-79
Author(s):  
Hasriadi Mat Akin
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Untranslated Region ◽  
Protein Gene ◽  
Dna Probe ◽  
Nuclear Inclusion ◽  
Chain Reaction ◽  
Peanut Stripe Virus ◽  
Polymerase Chain ◽  
Labeled Probe

The use of nonradioactive probe (Digoxigenin-DNA)  for  detection of peanut stripe virus.  The objective of this experiment was to develop the nonradioactive-labeled probe to detect peanut stripe virus (PStV) in peanut leaves and seeds. Digoxigenin labeled cDNA (dig-DNA probe) was synthesized from recombinant plasmid (pHS1.23) using polymerase chain reaction (PCR).  The probe containing 1.195 bp (base pair) corresponding to 3' termini, included part of NIb (nuclear inclusion body) gene, coat protein gene, and 3' untranslated region of PStV genome was used to detect the existence of PStV in peanut leaves and seeds of infected peanut plants.  

scholarly journals Resistance of Transgenic Prunus domestica to Plum Pox Virus Infection

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1231-1235 ◽  
Author(s):  
M. Ravelonandro ◽  
R. Scorza ◽  
J. C. Bachelier ◽  
G. Labonne ◽  
L. Levy ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Coat Protein ◽  
Plum Pox Virus ◽  
Prunus Domestica ◽  
Chain Reaction ◽  
Cp Gene ◽  
Aphid Feeding ◽  
Polymerase Chain ◽  
Dormancy Cycles ◽  
Das Elisa

Transgenic plum trees (Prunus domestica) containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV by aphid feeding or chip budding. Infection was monitored by evaluation of virus symptoms, DAS-ELISA, and immunoblot assays. Based on observations and analyses over 3 years including two dormancy cycles, one out of five transgenic clones (C-5), was found to be resistant to infection whether inoculated by aphids or by chip budding. PPV could not be detected in any inoculated plants of the C-5 clone by immunoblot or immunocap-ture-reverse transcriptase-polymerase chain reaction assays. To our knowledge, this is the first P. domestica clone resistant to PPV infection produced by genetic engineering.

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