The application of micro grafting technique in micro propagation of cherry root stock cultivar

2021 ◽  
Vol 11 (5) ◽  
pp. 145-150
Author(s):  
Saimnazarov Yuldash Bekmirzayevich ◽  
Abduramanova Salomat Khudaybergenovna
Keyword(s):  
Grafting Technique ◽  
Root Stock ◽  
Micro Propagation

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

BIOASSAY TO DETERMINE CHERRY ROOTSTOCK TOLERANCE TO `BACTERIAL CANKER' CAUSED BY PSEUDOMONAS SYRINGAE PV SYRINGAE

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1354F-1355
Author(s):  
Elzbieta Krzesinska ◽  
Anita Nina Miller
Keyword(s):  
Relative Humidity ◽  
Pseudomonas Syringae ◽  
Bacterial Suspension ◽  
Avirulent Strain ◽  
Bacterial Canker ◽  
Symptom Development ◽  
Virulent Strains ◽  
Callus Production ◽  
Root Stock ◽  
One Year

An excised twig assay was developed to evaluate cherry geno-types for their tolerance to Pseudomonas syringae pv. syringae. One-year-old wood was collected at monthly intervals from October until January of `Royal Ann', `Corum', and a number of cherry rootstock. The rootstock included; F/12–1 and Giessen (GI) and M × M selections. A 2-cm incision (“^”-shaped flap) was made on each twig. A 20-μl droplet of inoculum or water was placed onto each incision. The inoculum was prepared with one avirulent (K4) and three virulent strains (W4N54, AP2, B15) concentrations (105, 106, or 107 cfu). Inoculated twigs were placed in test tubes and incubated at 15C in high relative humidity for 3 weeks. After incubation, twigs were evaluated for gummosis production (0–3, 0 = no gummosis), incision browning (1–4, 1 = yellow pith), and callus production (0–1, 0 = no callus). The concentration of bacterial suspension had no effect on symptom development. No gummosis or browning was observed on twigs inoculated with water or the avirulent strain. Based on the gummosis and browning ratings, rootstock M × M 2, M × M 39, M × M 60, GI 148-1, GI 154-2, and GI 154-4 were found to be resistant to these three strains of P. syringae in this assay. Root-stock F 12-1, GI 169–15, GI 172–9, and GI 173-9 were found to be tolerant.

Follow-up examinations of patients after endovascular aortic grafting – Technique, requirements, and complications

2001 ◽  
Vol 6 (4) ◽  
pp. 185-193 ◽  
Author(s):  
T. Umscheid ◽  
M. Skupin ◽  
B. Unkel ◽  
G. Rouhani ◽  
C. Wiedeck ◽  
...  
Keyword(s):  
Grafting Technique

Lightbulb technique for the treatment of osteonecrosis of the femoral head

2017 ◽  
Vol 28 (3) ◽  
pp. 272-277 ◽  
Author(s):  
Cemil Yildiz ◽  
Yusuf Erdem ◽  
Kenan Koca
Keyword(s):  
Femoral Head ◽  
Disease Progression ◽  
Bone Grafting ◽  
Treatment Effectiveness ◽  
Treatment Success ◽  
Total Hip ◽  
Kaplan Meier ◽  
Grafting Technique ◽  
Stage 4 ◽  
Stage 1

Introduction: The aim of this study was to report the clinical and radiological outcomes for 21 patients (28 hips) treated for osteonecrosis of the femoral head using the lightbulb technique, a nonvascularised bone grafting technique. Methods: The study group included 14 men and 7 women, with a mean age of 33.2 (range 22-50) years, presenting with avascular necrosis of the femoral head of stage 4a or earlier, according to the Steinberg classification. Patients were treated using the nonvascularised lightbulb bone grafting technique. The primary clinical outcome was the Harris Hip Scores (HHS), while primary outcomes of treatment effectiveness and disease progression were based on radiographic evidence of disease progression and the need for total hip replacement. The rate of treatment success and failure was evaluated using the Kaplan-Meier survival analysis. Results: The mean HHS increased from 52.66 to 74.33 after treatment, with excellent-to-good outcomes obtained in 21 (75%) of the cases. Fair-to-poor results were obtained in 7 (25%) of the cases, with total hip arthroplasty subsequently required in 5 of these cases. The radiological failure rate was 50% among cases treated in Steinberg stage 1 (1/2), 42% in stage 3 (5/12), and 100% in stage 4 (2/2). Conclusions: The lightbulb technique can provide a clinically acceptable rate of successful treatment of osteonecrosis of the femoral head when used in the early stages of the disease, prior to collapse of the femoral head.

scholarly journals PRODUCTION OF GUAVA MINI-GRAFTED ON INTRA OR INTERSPECIFIC ROOTSTOCK

2017 ◽  
Vol 39 (1) ◽  
Author(s):  
GRAZIELLA SIQUEIRA CAMPOS ◽  
CLÁUDIA SALES MARINHO ◽  
CAMILLA RANGEL PORTELLA ◽  
BRUNO DIAS AMARAL ◽  
WALESKA SOARES GOMES DE CARVALHO
Keyword(s):  
Experimental Design ◽  
Psidium Guajava ◽  
Production Time ◽  
Fruit Species ◽  
Grafting Technique ◽  
Average Production ◽  
Increase Efficiency

ABSTRACT Mini-grafting is a type of grafting that has been indicated to increase efficiency of forest and fruit species propagation. The aim of this study was to evaluate the mini-grafting technique as a method for propagation of guava grafted on intra or interspecific rootstock. The experimental design was randomized blocks, with four treatments, five replications and plots consisting of ten plants, in a 22 factorial arrangement, and factors consisted of rootstocks and canopy cultivars. Rootstocks used were Psidium guajava and Psidium guineense. The canopy used was Paluma and Cortibel 1 cultivars. Only thecanopy used had an effect on the percentage of grafting success of 52, 54, 82 and 84%, respectively, for ‘Cortibel 1’/P. guineense; ‘Cortibel 1’/P. guajava; ‘Paluma’/P. guineense; ‘Paluma’/P. guajava combinations. Mini-grafting was effective for guava propagation on intra or interspecific rootstocks. The average production time for ‘Paluma’ and ‘Cortibel 1’ saplings grafted on P. guajava or P. guineense, for mini-grafting, was 351 days, so that ‘Paluma’ seedlings were more vigorous than those of ‘Cortibel 1’.

Treating Anisomastia and Tuberous Breast with Fat Grafting: Technique and Evaluation of Outcomes Using BREAST-Q Surveys

2021 ◽  
Author(s):  
Sarantos Papadopoulos ◽  
Steven D. M. Colpaert ◽  
Dimitrios G. Goulis ◽  
Meletios P. Nigdelis ◽  
Grigorios F. Grimbizis ◽  
...  
Keyword(s):  
Fat Grafting ◽  
Tuberous Breast ◽  
Grafting Technique

Comparing the Missouri Gravel Bed and a Wood Chip Production Method for Tree Growth

2019 ◽  
Vol 45 (6) ◽  
Author(s):  
Kelby Fite ◽  
Liza Holmes ◽  
Elden LeBrun
Keyword(s):  
Wood Chip ◽  
Production Method ◽  
White Oak ◽  
Gravel Bed ◽  
System A ◽  
Nursery Production ◽  
Quercus Bicolor ◽  
Root Stock ◽  
Swamp White Oak ◽  
Bare Root

Tree root defects from current nursery production practices influence short- and long-term tree performance and survivability. The Missouri Gravel Bed (MGB) system, a production method using gravel as a substrate, has been used to prevent many of these defects from occurring. MGB production involves planting bare root stock into a bed of gravel with frequent drip irrigation in order to produce a root system with relatively few defects. MGB production methods have also been purported to allow for summer transplanting of many species, as opposed to traditional dormant transplanting.Because gravel has low water- and nutrient-holding capacity, biochar (5% by volume) was incorporated into one plot as a possible means of improving both water- and nutrient-holding capacity over gravel alone. Wood chip mulch was also investigated as a growing substrate in place of the gravel in a growing system. In 2015, three species, Quercus bicolor (swamp white oak), Taxodium distichum (baldcypress), and Tilia cordata (littleleaf linden), were studied in pea gravel (PG), biochar-amended pea gravel (BC), and wood chip mulch bed (MB) growing environments. Very few differences occurred over the growing season with above- or belowground parameters indicating that the minimal-to-no-cost, more readily available substrate of wood chip mulch should be considered in these growing systems.

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