Activity of Enzymatic Antioxidants and Total Antioxidant Capacity in Seminal Plasma of Murrah Bulls during Cryopreservation

2016 ◽  
Vol 6 (3) ◽  
pp. 405 ◽  
Author(s):  
S.A. Lone ◽  
J.K. Prasad ◽  
S.K. Ghosh ◽  
G.K. Das ◽  
B. Balamurugan ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Enzymatic Antioxidants ◽  
Total Antioxidant

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

scholarly journals High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Isabel Barranco ◽  
Asta Tvarijonaviciute ◽  
Cristina Perez-Patiño ◽  
Inmaculada Parrilla ◽  
Jose J. Ceron ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant ◽  
Sperm Survival

1063 Automated measurement of total antioxidant capacity of seminal plasma

2005 ◽  
Vol 4 (3) ◽  
pp. 268
Author(s):  
D. Unal ◽  
H. Ciftci ◽  
M. Savas ◽  
M. Gulum ◽  
E. Yeni ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Automated Measurement ◽  
Total Antioxidant

scholarly journals Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

Apidologie ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 227-236 ◽  
Author(s):  
Mariola Słowińska ◽  
Joanna Nynca ◽  
Jerzy Wilde ◽  
Beata Bąk ◽  
Maciej Siuda ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant

scholarly journals Hormonal Regulation of Total Antioxidant Capacity in Seminal Plasma

2009 ◽  
Vol 30 (5) ◽  
pp. 534-540 ◽  
Author(s):  
A. Mancini ◽  
R. Festa ◽  
A. Silvestrini ◽  
N. Nicolotti ◽  
V. Di Donna ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Hormonal Regulation ◽  
Total Antioxidant

scholarly journals 266 ANTIOXIDANT CAPACITY OF BOAR SEMINAL PLASMA

2005 ◽  
Vol 17 (2) ◽  
pp. 283 ◽  
Author(s):  
M. Hernandez ◽  
A. Cano ◽  
M.B. Arnao ◽  
X. Lucas ◽  
J.M. Vazquez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Room Temperature ◽  
Color Characteristics ◽  
Nylon Mesh ◽  
Total Antioxidant ◽  
Lipophilic Antioxidant

It has been established that antioxidants in seminal plasma play an important role in protecting the spermatozoa against oxidative stress-induced damage. This study was conducted to measure the total antioxidant capacity (TAC) of boar seminal plasma. Fifty-four ejaculates were collected from 17 mature boars of proven fertility by the gloved-hand technique. Ejaculates were collected separately in different fractions (pre-sperm, sperm-rich, and post-sperm) according to their macroscopic (color) characteristics. After centrifugation (2400g for 3 min), the sperm pellet was discarded; the supernatant was recentrifuged and filtered through a 10-μm nylon mesh filter to remove debris or clumped spermatozoa. The seminal plasma was frozen at -20°C until further use. After thawing at room temperature, seminal plasma aliquots of 5 μL were immediately assessed for total antioxidant capacity. TAC was measured using the ABTS/H2O2/HRP decoloration method (Cano A et al. 2000 Redox Report 5, 365–370) which allows differentiation between hydrophilic and lipophilic antioxidant activity capacity. TAC units were expressed as micromolar (μM) Trolox equivalents. Data were analyzed using ANOVA. Only the hydrophilic activity was measurable, with the lipophilic activity being undetected. The overall TAC of seminal samples (mean ± SEM) was 1623.7 ± 56.28 μM, ranging from 674 to 2428 μM. Different TACs were observed among males (P < 0.05) and between ejaculates of the same male (P < 0.05). Ejaculate fraction had a significant effect (P < 0.001) on the TAC levels. The post-sperm fraction had a significantly lower TAC level (1104.09 ± 57.66 μM) than the pre-sperm and sperm-rich fractions (1611.95 ± 153.68 μM and 1356.136 ± 72.47 μM, respectively, P < 0.001). In conclusion, hydrophilic antioxidant activity represented the main contribution to the TAC in boar seminal plasma, showing differences among males, between ejaculates of the same male, and also between the different ejaculate fractions. This work was supported by CICYT (AGF98-0533; AGL01-0471) and INIA (RZ01-019).

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