Microsatellite locus Ame17 polymorphism for round goby (Neogobius melanostomus) from Northwest Black Sea region

Aim. To identify the Ame17 polymorphic variants for round goby Neogobius melanostomus from Northwest Black Sea region. Methods. We determined polymorphism for 102 individuals of round goby. Genotyping was performed by PCR-analysis of Ame17 as described by Brown et al., 2008. The PCR-amplified DNA products were subjected to electrophoresis in 8 % polyacrylamide gel. Results. There have been identified 23 alleles. The range of repeat sizes was from 140 to 300 bp. The average heterozygosity was 0.76. Conclusions. There have been analyzed polymorphisms of Ame17 microsatellite locus for round goby from Northwest Black Sea region. The highest genetic diversity at Ame17 locus was observed for fish from the Gulf of Odessa. In terms of heterozygosity subpopulation of round goby from Bay Dzharylgach was significantly different from other localities. Keywords: round goby, polymorphism, microsatellite loci, Ame17.

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Genetic Differentiation of the Round Goby (Neogobius melanostomus) from Certain Localities of the Black and Azov Sea Basin Using Microsatellite Loci

Cytology and Genetics ◽

10.3103/s0095452721030105 ◽

2021 ◽

Vol 55 (3) ◽

pp. 236-247

Author(s):

S. I. Tarasjuk ◽

V. V. Zamorov ◽

O. V. Zaloilo ◽

O. Iu. Bielikova ◽

D. B. Radionov

Keyword(s):

Genetic Differentiation ◽

Microsatellite Loci ◽

Round Goby ◽

Neogobius Melanostomus ◽

Azov Sea

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Genetic diversity of old bread wheat germplasm from the Black Sea region evaluated by microsatellites and agronomic traits

Plant Genetic Resources ◽

10.1017/s1479262114000781 ◽

2014 ◽

Vol 13 (2) ◽

pp. 119-130 ◽

Cited By ~ 5

Author(s):

Svetlana Landjeva ◽

Ganka Ganeva ◽

Viktor Korzun ◽

Dean Palejev ◽

Sabina Chebotar ◽

...

Keyword(s):

Genetic Diversity ◽

Bread Wheat ◽

Black Sea ◽

Agronomic Traits ◽

Polymorphic Information Content ◽

Group Differences ◽

The Black Sea ◽

Black Sea Region ◽

Western Group ◽

New Alleles

Old germplasm is an important genetic resource for enhancing modern crops with new alleles. In the present study, the genetic diversity of 52 historic varieties and landraces of bread wheat originated from the Western (Bulgaria) and Northeastern (Ukraine, Russia and Georgia) regions of the Black Sea basin was assessed based on microsatellite markers and agronomic characteristics. A set of 24 markers detected a total of 263 alleles at 25 microsatellite loci, with an average number of 10.5 alleles per locus and an average polymorphic information content (PIC) of 0.74. A total of 63 alleles at 22 loci were unique, being specific to a particular accession. Half of the alleles (132) were regionally specific, and the rest were common between the Western and Northeastern accessions. The latter group was characterized with greater total and private allelic richness, a higher number of unique alleles and a higher average PIC. The population was found to be very heterogeneous (average heterogeneity 41%), with the Northeastern pool (52.8%) being more diverse than the Western pool (30.9%). Most of the accessions of the Western group clustered together, and the rest were distributed among the subclusters of the Northeastern germplasm. Large inter-group differences in the frequencies of alleles ranging from 3.1 at Xgwm294-2A to 16.7 at Xgwm333-7B were observed. This variation might partly account for the differences in certain yield-related traits. The Northeastern accessions had significantly longer spikes with more number of spikelets. Some issues related to germplasm preservation in seed genebanks are discussed herein. The large molecular variation observed could be utilized by breeders for the selection of diverse parents, or by researchers for the production of mapping populations.

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Shape Analysis of Otoliths of the Round goby, Neogobius melanostomus (Gobiiformes, Gobiidae), from the Black Sea Basin

Zoodiversity ◽

10.15407/zoo2021.04.317 ◽

2021 ◽

Vol 55 (4) ◽

pp. 317-330

Author(s):

V. Zamorov ◽

M. Zamorova ◽

D. Krupko ◽

N. Matvienko ◽

Y. Leonchyk ◽

...

Keyword(s):

Discriminant Analysis ◽

Black Sea ◽

Sagittal Plane ◽

Round Goby ◽

Neogobius Melanostomus ◽

The Black Sea ◽

Strong Response ◽

Linear Discriminant ◽

The North ◽

Round Gobies

The aim of this study was to assess the discriminability of the stocks of the round goby Neogobius melanostomus based on the shape of its otoliths. Recent otolith-shape-based species and stock discrimination studies were using otolith contours in sagittal plane and we are following this approach. We hypothesized the possibility of existence of several geographically separated populations of the round goby. Round gobies have been sampled from different locations of the North-Western Black Sea, otoliths were removed in course of the full biological analysis and photographed in sagittal plane. Principal components of the otolith contour were processed by linear discriminant analysis aiming to cross-validate the discriminability of round gobies placed at different geographical locations. This would allow demonstration of different stocks or populations. This research allows to conclude the limited applicability of otolith contours for discrimination of stocks or populations of round goby based on multiple annual samples. However, neither classification matrices of discriminant analysis nor cluster analysis dendrograms showed a single pattern except for the high year to year otoliths variability. This allows to hypothesise a strong response of contour formation to habitat and feeding conditions. However, this assumption needs to be verified by further studies.

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The Review of Ecological and Genetic Research of Ponto-Caspian Gobies (Pisces, Gobiidae) in Europe

Croatian Journal of Fisheries ◽

10.1515/cjf-2016-0015 ◽

2016 ◽

Vol 74 (3) ◽

pp. 110-123 ◽

Cited By ~ 1

Author(s):

Goran Jakšić ◽

Margita Jadan ◽

Marina Piria

Keyword(s):

Black Sea ◽

Dietary Habits ◽

Gene Diversity ◽

Genetic Research ◽

Round Goby ◽

Morphological Characteristics ◽

Neogobius Melanostomus ◽

Age And Growth ◽

The Black Sea ◽

Fish Assemblage Structure

Abstract Invasive Ponto-Caspian gobies (monkey goby Neogobius fluviatilis, round goby Neogobius melanostomus and bighead goby Ponticola kessleri) have recently caused dramatic changes in fish assemblage structure throughout European river systems. This review provides summary of recent research on their dietary habits, age and growth, phylogenetic lineages and gene diversity. The principal food of all three species is invertebrates, and more rarely fish, which depends on the type of habitat, part of the year, as well as the morphological characteristics of species. According to the von Bertalanffy growth model, size at age is specific for the region, but due to its disadvantages it is necessary to test other growth models. Phylogenetic analysis of monkey goby and round goby indicates separation between the Black Sea and the Caspian Sea haplotypes. The greatest genetic diversity is found among populations of the Black Sea, and the lowest among European invaders. The lack of molecular research on bighead goby requires further studies.

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