Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.

Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.

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The effect of mutations in the alleles Gli-B1b and Gli-B1l on grain quality indices of common wheat

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v27.1309 ◽

2020 ◽

Vol 27 ◽

pp. 94-99

Author(s):

N. A. Kozub ◽

I. O. Sozinov ◽

H. Ya. Bidnyk ◽

N. O. Demianova ◽

O. I. Sozinova ◽

...

Keyword(s):

Protein Content ◽

Common Wheat ◽

Grain Quality ◽

Storage Proteins ◽

Polyacrylamide Gel Electrophoresis ◽

Grain Protein Content ◽

Grain Hardness ◽

Quality Indices ◽

Sedimentation Volume ◽

Mutant Lines

Aim. The aim of this study was to analyze quality indices in mutants at gliadin loci developed on the basis of the common wheat cultivar Bezostaya 1. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to check mutations. Grain quality indices (SDS sedimentation volume, grain hardness, seed plumpness, and grain protein content) were analyzed: Results. Both mutants at Gli-R1 with the intensified secalin component and with the changed mobility of the secalin component showed lower grain hardness in comparison with the respective non-mutant lines. The mutation of the allele Gli-B1b causing the absence of synthesis of the g-gliadin and the minor w-gliadin resulted in the decrease in grain hardness and in the increase in the SDS-sedimentation volume. Seeds of the Gli-B1b mutant with the absence of synthesis of the major w-gliadin were less plump and showed higher protein content than in the non-mutant line. Conclusions. The effects of mutations of the allele Gli-B1b associated with the absence of certain gliadin components and Gli-R1 mutations on grain quality indices were revealed. Keywords: Triticum aestivum, gliadins, secalins, SDS-sedimentation, grain hardness.

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Development and molecular characterization of wheat – Aegilops kotschyi addition and substitution lines with high grain protein, iron, and zinc

Genome ◽

10.1139/g11-059 ◽

2011 ◽

Vol 54 (11) ◽

pp. 943-953 ◽

Cited By ~ 33

Author(s):

Nidhi Rawat ◽

Kumari Neelam ◽

Vijay K. Tiwari ◽

Gursharn S. Randhawa ◽

Bernd Friebe ◽

...

Keyword(s):

Molecular Weight ◽

Protein Content ◽

High Molecular Weight ◽

Interspecific Cross ◽

Grain Protein Content ◽

Grain Protein ◽

Substitution Lines ◽

Iron And Zinc ◽

Zinc Concentrations ◽

Recurrent Backcrossing

Over two billion people, depending largely on staple foods, suffer from deficiencies in protein and some micronutrients such as iron and zinc. Among various approaches to overcome protein and micronutrient deficiencies, biofortification through a combination of conventional and molecular breeding methods is the most feasible, cheapest, and sustainable approach. An interspecific cross was made between the wheat cultivar ‘Chinese Spring’ and Aegilops kotschyi Boiss. accession 396, which has a threefold higher grain iron and zinc concentrations and about 33% higher protein concentration than wheat cultivars. Recurrent backcrossing and selection for the micronutrient content was performed at each generation. Thirteen derivatives with high grain iron and zinc concentrations and contents, ash and ash micronutrients, and protein were analyzed for alien introgression. Morphological markers, high molecular weight glutenin subunit profiles, anchored wheat microsatellite markers, and GISH showed that addition and substitution of homoeologous groups 1, 2, and 7 chromosomes of Ae. kotschyi possess gene(s) for high grain micronutrients. The addition of 1U/1S had high molecular weight glutenin subunits with higher molecular weight than those of wheat, and the addition of 2S in most of the derivatives also enhanced grain protein content by over 20%. Low grain protein content in a derivative with a 2S-wheat translocation, waxy leaves, and absence of the gdm148 marker strongly suggests that the gene for higher grain protein content on chromosome 2S is orthologous to the grain protein QTL on the short arm of group 2 chromosomes.

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Pyramiding of Genes for Grain Protein Content, Grain Quality and Rust Resistance in Eleven Indian Bread Wheat Cultivars: A Multi-Institutional Effort

10.21203/rs.3.rs-637558/v1 ◽

2021 ◽

Author(s):

Pushpendra K. Gupta ◽

Harindra S. Balyan ◽

Parveen Chhuneja ◽

Jai P. Jaiswal ◽

Shubhada Tamhankar ◽

...

Keyword(s):

Protein Content ◽

Rust Resistance ◽

Grain Quality ◽

Grain Protein Content ◽

Grain Protein ◽

Loaf Volume ◽

Wheat Cultivars ◽

Glutenin Subunits ◽

Hmw Glutenin Subunits ◽

Hmw Glutenin

Abstract Improvement of grain protein content (GPC), loaf volume and resistance to rusts was achieved in 11 Indian wheat cultivars that are widely grown in four different agro-climatic zones of India. This involved use of marker-assisted backcrossing (MABC) for introgression and pyramiding of the following genes: (i) the high GPC gene Gpc-B1; (ii) HMW glutenin subunits 5 + 10 at Glu-D1 loci, and (iii) rust resistance genes, Yr36, Yr15, Lr24 and Sr24. GPC was improved by 0.8–3.3%, although high GPC was generally associated with yield penalty. Further selection among high GPC lines, allowed development of progenies with higher GPC associated with improvement in 1000-grain weight and grain yield in the following four cultivars: NI5439, UP2338, UP2382 and HUW468. The high GPC progenies (derived from NI5439) were also improved for grain quality using HMW glutenin subunits 5 + 10 at Glu-D1 loci. Similarly, progenies combining high GPC and rust resistance were developed in the backgrounds of following five cultivars: Lok1, HD2967, PBW550, PBW621 and DBW1. The improved pre-bred lines developed during the present study should prove useful for development of cultivars with improved nutritional quality associated with rust resistance in future wheat breeding programmes.

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Grain quality of common wheat lines with T. kiharae genetic material

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v23.998 ◽

2018 ◽

Vol 23 ◽

pp. 108-113

Author(s):

O. A. Orlovskaya ◽

S. I. Vakula ◽

L. V. Khotyleva ◽

A. V. Kilchevsky

Keyword(s):

Molecular Weight ◽

Common Wheat ◽

Grain Quality ◽

Genetic Material ◽

Glutenin Subunits ◽

Gluten Quality ◽

Gluten Content ◽

Sds Page ◽

Wheat Lines

Aim. T. kiharae (AtAtGGDD, 2n=42) is a source of high protein and gluten content, resistance to many diseases. Сommon wheat lines with the introgression of T. kiharae genetic material were obtained in order to enrich T. aestivum L. gene pool. The aim of this study was to assess the impact of T. kiharae genetic material on the grain quality of T. aestivum/T. kiharae introgression lines. Methods. The composition of the high molecular weight glutenin subunits was analyzed by SDS-PAGE. Evaluation of the most important traits of grain quality (hardness, protein and gluten content, gluten quality) was carried out according to GOST. Results. Сomparative analysis of the composition of high molecular weight glutenin subunits of introgressive lines and their parental forms allowed us to identify lines with novel alleles of Glu-1 loci, specific for T. kiharae. For most of the introgression lines T. aestivum/T. kiharae hardness, protein and gluten content were higher than for parent wheat varieties. Conclusions. Introgression of T. kiharaegenetic material in the genome of common wheat had a positive effect on all studied parameters of grain quality except the gluten quality. Keywords: common wheat, T. kiharae, glutenin, SDS-PAGE, quality of grain.

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Grain Protein Content in Wheat: Genetics of the Character and Some Predictions for Its Improvement in Common Wheat

Russian Journal of Genetics ◽

10.1007/s11177-005-0167-9 ◽

2005 ◽

Vol 41 (8) ◽

pp. 823-834 ◽

Cited By ~ 4

Author(s):

A. V. Zlatska

Keyword(s):

Protein Content ◽

Common Wheat ◽

Grain Protein Content ◽

Grain Protein

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Molecular genetic analysis of grain protein content and flour whiteness degree using RILs in common wheat

Journal of Genetics ◽

10.1007/s12041-016-0639-9 ◽

2016 ◽

Vol 95 (2) ◽

pp. 317-324 ◽

Cited By ~ 4

Author(s):

XIANYIN SUN ◽

KE WU ◽

YAN ZHAO ◽

ZHAOGUO QIAN ◽

FANMEI KONG ◽

...

Keyword(s):

Genetic Analysis ◽

Protein Content ◽

Common Wheat ◽

Molecular Genetic ◽

Grain Protein Content ◽

Molecular Genetic Analysis ◽

Grain Protein

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EFFECT OF TILLAGE METHODS AND LEVELS OF MINERAL NUTRITION ON YIELD AND GRAIN QUALITY OF THE “VLADIMIR” SPRING BARLEY VARIETY IN THE CENTRAL REGION OF THE NON-CHERNOZEM ZONE

Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii ◽

10.26897/0021-342x-2021-1-18-28 ◽

2021 ◽

pp. 18-28

Author(s):

А.N. FILATOV ◽

◽

V.N. MAZUROV ◽

V.K. KHRAMOY ◽

E.R. ARLANTSEVA

Keyword(s):

Protein Content ◽

Mineral Nutrition ◽

Grain Quality ◽

Spring Barley ◽

Mineral Fertilizer ◽

Grain Protein Content ◽

Barley Grain ◽

Mineral Fertilizers ◽

Grain Protein

Providing the brewing industry with locally produced raw materials is important both from an economic point of view and in terms of import substitution. Breeders create brewing barley varieties with a reduced protein content, however, in production they often use universal varieties that combine high yields with an average protein content of 11–12%. For such varieties, it is necessary to develop technological methods for obtaining a crop with specified properties, depending on the purposes of using the products. The quality indicators of barley grain and the efficiency of its production are significantly influenced by mineral fertilizers and the minimization of soil cultivation. In this regard, the authors studied the effect of minimal tillage on the yield and grain quality of spring barley of the Vladimir variety at two levels of mineral nutrition (without fertilizers 26 and with full supply oif mineral fertilizer – N35P40K135) in a two-factor field experiment carried out in 2018–2019 on the medium-loamy light gray forest soil. The experiment helped determine the yield and structure of the crop, the content of protein, starch, and grain hoodness (hull content). It was found that the lack of precipitation during the barley tillering – heading period leads to a decrease in grain yield by 40.0–54.8% as compared with the conditions of increased moisturizing. At the same time, there was an increase in the grain protein content by 1.4–1.7% and the grain filminess by 0.5–0.9%. The use of moderate rates of complete mineral fertilizer (N35P40K85) led to an increase in the barley yield by an average of 13.2% and the grain protein content by an average of 0.2%. On the other hand, it led to a decrease in the grain filminess by 0.2% and starch content by 0.4–0.6%. The positive effect of nitrogen fertilizers on the increase in protein content in barley grain was higher under minimal tillage. Under minimal tillage, regardless of the level of moisture supply and mineral nutrition, there was a tendency towards a decrease in the barley yield by an average of 3.9%, mainly due to a decrease in the density of the productive stalk. There was also a tendency towards a decrease in the protein content in the barley grain – by 0.3% – when no complete mineral fertilizer was used.

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