Effect of Gpc-B1 gene on grain protein content and productivity traits in a set of high yielding wheat lines

A set of 94 BC2F7 lines (BC RILs), selected for presence of Gpc-B1 gene from the cross Glupro/3*PBW568 were evaluated for morpho-physiological characters including yield and its components, grain protein content, grain zinc and iron concentration for two seasons. Gpc-B1 mediated grain protein enhancement as influenced by combination of other agronomic traits was studied through inter-trait correlations. Grain protein content was positively correlated with plant height (r=0.552**, 0.676**) and 1000-grain weight (r=0.257**, 0.287**) in both years. Lines with high yield conferring combinations of yield components showed lower grain protein content. The results showed that there were no constraints for combining grain weight with Gpc- B1 mediated proteins enhancement provided yield moderation can be exercised. Grain protein contents showed significant and positive correlation with grain zinc (0.557**, 0.764**) and iron concentration (0.542**, 0.595**) indicating that Gpc-B1 mediated enhancement in protein content and not just presence of Gpc-B1 was necessary. Zinc and iron concentration were also positively associated with each other (0.567**, 0.648**).

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Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat

Frontiers in Plant Science ◽

10.3389/fpls.2019.00464 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 5

Author(s):

Jia Liu ◽

Lin Huang ◽

Changquan Wang ◽

Yaxi Liu ◽

Zehong Yan ◽

...

Keyword(s):

Association Study ◽

Protein Content ◽

Genome Wide Association Study ◽

Grain Protein Content ◽

Wild Emmer ◽

Genome Wide Association ◽

Grain Protein ◽

Genome Wide ◽

Genomic Regions ◽

Wheat Lines

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High-yielding lines of wheat carrying Gpc-B1 adapted to Mediterranean-type environments of the south and west of Australia

Crop and Pasture Science ◽

10.1071/cp14106 ◽

2014 ◽

Vol 65 (9) ◽

pp. 854 ◽

Cited By ~ 20

Author(s):

H. A. Eagles ◽

Robyn McLean ◽

R. F. Eastwood ◽

M.-J. Appelbee ◽

Karen Cane ◽

...

Keyword(s):

Grain Yield ◽

Protein Content ◽

Heading Date ◽

Grain Protein Content ◽

Grain Weight ◽

Wild Emmer ◽

Grain Protein ◽

The South ◽

B1 Gene ◽

Elite Cultivar

The Gpc-B1 gene from wild emmer has been proposed as a potential mechanism for improving grain protein in bread wheat without reducing grain yield. Near-isolines with and without the Gpc-B1 gene in three Australian-adapted genetic backgrounds, Gladius, Wyalkatchem and VR1128, were compared in 14 experiments across the south and west of Australia for grain yield, grain protein content and grain weight. The donor parents of Gpc-B1 were the Canadian cultivars Burnside and Somerset. One of the 14 experiments was discarded because of inadequate rust control and confounding effects of Yr36, a gene closely linked to Gpc-B1. Heading date and test weight were measured in five experiments. Across all comparisons, Gpc-B1 increased grain protein content and reduced grain weight, with a negligible effect on grain yield. Selected lines containing Gpc-B1 in a Wyalkatchem background had comparable grain yields to the elite cultivar Mace, but with significantly higher grain protein contents, slightly higher grain weights, similar heading dates and acceptable test weights. The development of agronomically acceptable lines containing Gpc-B1 was partially attributed to the removal of undesirable genes from wild emmer during the breeding of the Canadian donor parents and the use of Australian recurrent parents with high test weights.

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Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v29.1412 ◽

2021 ◽

Vol 29 ◽

pp. 87-91

Author(s):

N. A. Kozub ◽

I.O. Sozinov ◽

H.Ya. Bidnyk ◽

N.O. Demianova ◽

O.I. Sozinova ◽

...

Keyword(s):

Molecular Weight ◽

Protein Content ◽

High Molecular Weight ◽

Common Wheat ◽

Grain Quality ◽

Grain Protein Content ◽

Grain Protein ◽

Quality Indices ◽

Sedimentation Volume ◽

Wheat Lines

Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.

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Estimation of NAM-A1 haplotypes effect on the level of quantitative traits and grain protein content in wheat

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v26.1252 ◽

2020 ◽

Vol 26 ◽

pp. 114-119

Author(s):

O. A. Orlovskaya ◽

S. I. Vakula ◽

L. V. Khotyleva ◽

A. V. Kilchevsky

Keyword(s):

Protein Content ◽

Bread Wheat ◽

Quantitative Traits ◽

Grain Protein Content ◽

Kernel Weight ◽

Grain Protein ◽

Wheat Varieties ◽

Vegetative Organs ◽

Thousand Kernel Weight ◽

Wheat Lines

Aim. NAM-A1 gene plays an important role in the regulation of metabolic processes, the remobilization of nutrients from the wheat vegetative organs to grain. The aim of this work is the genotyping of SNP polymorphisms of the NAM-A1 gene in introgression lines derived from the cross of bread wheat varieties with species of genus Triticum, as well as estimation of NAM-A1 haplotypes effect on quantitative traits and grain protein content of the studied genotypes. Methods. NAM-A1 gene polymorphisms were identified using the TaqMan - Real-time PCR. Statistical data analysis was carried out using MS Excel 2007, Statistica 10.0. Results. NAM-A1c haplotype is mainly found in the studied genus Triticum species, NAM-A1d - in bread wheat varieties, and mixed haplotypes - in the introgression wheat lines. Statistically significant associations among NAM-A1 gene haplotypes and productive tillering of plants, the length of the main spike, the number of spikelets in the main spike and the thousand kernel weight were revealed. Conclusions. A significant effect of the NAM-A1 haplotypes was established for the traits «productive tillering» and «thousand kernel weight». The highest thousand kernel weight was reached in plants carrying the haplotype NAM-A1d. Keywords: T. aestivum L., species of genus Triticum, NAM-A1, SNP, quantitative characters.

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Relation of grain protein content and some agronomic traits in European cultivars of winter wheat

Cereal Research Communications ◽

10.1556/crc.2012.0004 ◽

2012 ◽

Vol 40 (4) ◽

pp. 532-541 ◽

Cited By ~ 1

Author(s):

V. Mladenov ◽

B. Banjac ◽

A. Krishna ◽

M. Milošević

Keyword(s):

Winter Wheat ◽

Protein Content ◽

Agronomic Traits ◽

Grain Protein Content ◽

Grain Protein

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Mutant Lines of Spring Wheat with Increased Iron, Zinc, and Micronutrients in Grains and Enhanced Bioavailability for Human Health

BioMed Research International ◽

10.1155/2019/9692053 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Saule Kenzhebayeva ◽

Alfia Abekova ◽

Saule Atabayeva ◽

Gulzira Yernazarova ◽

Nargul Omirbekova ◽

...

Keyword(s):

Genetic Variation ◽

Protein Content ◽

Spring Wheat ◽

Grain Protein Content ◽

Grain Protein ◽

Cereal Products ◽

Molar Ratios ◽

Stable Mutant ◽

Micronutrient Malnutrition ◽

Mutant Lines

Deficiency of metals, primarily Fe and Zn, affects over half of the world’s population. Human diets dominated by cereal products cause micronutrient malnutrition, which is common in many developing countries where populations depend heavily on staple grain crops such as wheat, maize, and rice. Biofortification is one of the most effective approaches to alleviate malnutrition. Genetically stable mutant spring wheat lines (M7 generation) produced via 100 or 200 Gy gamma treatments to broaden genetic variation for grain nutrients were analyzed for nutritionally important minerals (Ca, Fe, and Zn), their bioavailability, and grain protein content (GPC). Variation was 172.3–883.0 mg/kg for Ca, 40.9–89.0 mg/kg for Fe, and 22.2–89.6 mg/kg for Zn. In mutant lines, among the investigated minerals, the highest increases in concentrations were observed in Fe, Zn, and Ca when compared to the parental cultivar Zhenis. Some mutant lines, mostly in the 100 Gy-derived germplasm, had more than two-fold higher Fe, Zn, and Ca concentrations, lower phytic acid concentration (1.4–2.1-fold), and 6.5–7% higher grain protein content compared to the parent. Variation was detected for the molar ratios of Ca:Phy, Phy:Fe, and Phy:Zn (1.27–10.41, 1.40–5.32, and 1.78–11.78, respectively). The results of this study show how genetic variation generated through radiation can be useful to achieve nutrient biofortification of crops to overcome human malnutrition.

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